ARID1A/BAF250 recombinant antibody

Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody [BLR279L]

Cat. Num. AAA213681

• Gene Names: ARID1A; ELD; B120; OSA1; P270; hELD; BM029; MRD14; hOSA1; BAF250; C1orf4; BAF250a; SMARCF1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: ARID1A/BAF250, Recombinant Antibody; Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody [BLR279L]; osa homolog 1; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; hOSA1; hELD; MRD14; OSA1; OSA1 nuclear protein; P270; ELD; SWI/SNF complex protein p270; BRG1-associated factor 250; SWI-like protein; SMARCF1; B120; ARID domain-containing protein 1A; C1orf4; AT-rich interactive domain-containing protein 1A; CSS2; BAF250; BAF250a; BM029; brain protein 120; BRG1-associated factor 250a; chromatin remodeling factor p250; AT rich interactive domain 1A (SWI-like); AT-rich interaction domain 1A; ARID1A/BAF250 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR279L
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 50ug/ml (varies by lot)

• Applicable Applications for ARID1A/BAF250 recombinant antibody: FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 750 and 800
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 ug) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (AAA213681 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (AAA213681 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

ICC (Immunocytochemistry)

(Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (AAA213681 lot 1) used for IP at 20 ul/mg lysate. ARID1A/BAF250 was also immunoprecipitated by a second antibody against a different epitope of ARID1A/BAF250 . For blotting immunoprecipitated ARID1A/BAF250, AAA213681 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

FCM/FACS (Flow Cytometry)

(Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (AAA213681) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM/FACS (Flow Cytometry)

(Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (AAA213681) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for ARID1A/BAF250 recombinant antibody

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1A/BAF250 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1A/BAF250 has been shown to play a role in the tumor suppressor activity of the SWI/SNF complexes. ARID1A/BAF250 is required for differentiation-associated cell cycle arrest.

Product Categories/Family for ARID1A/BAF250 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 117968607
• NCBI GeneID: 8289
• NCBI Accession #: NP_006006.3
• NCBI GenBank Nucleotide #: NM_006015
• UniProt Accession #: O14497; Q53FK9; Q5T0W1; Q5T0W2; Q5T0W3; Q8NFD6; Q96T89; Q9BY33; Q9HBJ5; Q9UPZ1; D3DPL1
• Molecular Weight: 205,921 Da
• NCBI Official Full Name: Homo sapiens AT rich interactive domain 1A (SWI-like) (ARID1A), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: AT rich interactive domain 1A (SWI-like)
• NCBI Official Symbol: ARID1A
• NCBI Official Synonym Symbols: ELD; B120; OSA1; P270; hELD; BM029; MRD14; hOSA1; BAF250; C1orf4; BAF250a; SMARCF1
• NCBI Protein Information: AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; BRG1-associated factor 250a; OSA1 nuclear protein; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chroma
• UniProt Protein Name: AT-rich interactive domain-containing protein 1A
• UniProt Gene Name: ARID1A
• UniProt Synonym Gene Names: BAF250; BAF250A; C1orf4; OSA1; SMARCF1; ARID domain-containing protein 1A; BAF250; BAF250A; hOSA1
• UniProt Entry Name: ARI1A_HUMAN