Mouse Cytokeratin, pan Monoclonal Antibody | anti-KRT77 antibody

Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62765

• Gene Names: KRT77; K1B; KRT1B
• Reactivity: Human, Rat.; Shows broad species reactivity
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Cytokeratin, pan, Antibody; Cytokeratin, pan (Epithelial Marker) Mouse Monoclonal Antibody; anti-KRT77 antibody

• Host: Mouse
• Reactivity: Human, Rat.; Shows broad species reactivity
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: KRTL/1077 + KRTH/1076
• Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 578

• Applicable Applications for anti-KRT77 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic
• Immunogen: Recombinant human KRT77 and KRT76 protein
• Positive Control: Skin, Adeno- or Squamous carcinomas
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded Rat Oviduct with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).)

IHC (Immunohiostchemistry)

(Formalin paraffin Rat Stomach stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin, pan Monoclonal Antibody cocktail (KRTL/1077 + KRTH/1076).)

References

Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8.,2. Tseng SCG et. al. Cell 1982; 30361

NCBI and Uniprot Product Information

• NCBI GI #: 194018511
• NCBI GeneID: 374454
• NCBI Accession #: NP_778253.2
• NCBI GenBank Nucleotide #: NM_175078.2
• UniProt Accession #: Q7Z794; Q7RTS8
• Molecular Weight: 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
• NCBI Official Full Name: keratin, type II cytoskeletal 1b
• NCBI Official Synonym Full Names: keratin 77
• NCBI Official Symbol: KRT77
• NCBI Official Synonym Symbols: K1B; KRT1B
• NCBI Protein Information: keratin, type II cytoskeletal 1b
• UniProt Protein Name: Keratin, type II cytoskeletal 1b
• UniProt Gene Name: KRT77
• UniProt Synonym Gene Names: KRT1B; CK-1B; K77
• UniProt Entry Name: K2C1B_HUMAN