FCM/FACS (Flow Cytometry)
(Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody. Dilution: 1 u g /106 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE. Dilution: 1ug /test. Protocol: The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20?. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
Rabbit Pan Cytokeratin Polyclonal Antibody
Pan CytokeRatin
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
Applications
Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity purified by Protein A
Synonyms
Pan Cytokeratin, Antibody; Pan CytokeRatin; pan-cytokeratin; pan-CK; pan CK; P-CK; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 1; 2; 4; 5; 6; 7; 8; 71; 72; 75; 78]; anti-Pan Cytokeratin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-Pan Cytokeratin antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
KLH conjugated synthetic peptide derived from human cytokeratins 1, 2, 4, 5, 6, 7, 8, 71, 72, 75, 78
Conjugation
APC
Positive control
Rat bladder, Bladder (Mouse) Lysate, human cervical carcinoma, Hela, human gastric carcinoma, human liver, Human kidney, Human stomach carcinoma.
Subcellular location
Cytoplasmic.
Preparation and Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody. Dilution: 1 u g /106 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE. Dilution: 1ug /test. Protocol: The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20?. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Pan Cytokeratin antibody. Dilution: 1 u g /106 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE. Dilution: 1ug /test. Protocol: The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20?. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
WB (Western Blot)
(Bladder (Mouse) Lysate at 40 ug. Primary: Anti-Pan Cytokeratin at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42-64 kD. Observed band size: 60 kD.)
WB (Western Blot)
(Bladder (Mouse) Lysate at 40 ug. Primary: Anti-Pan Cytokeratin at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42-64 kD. Observed band size: 60 kD.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat bladder);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 ? for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4 ? ,followed by operating according to SP Kit(Rabbit) instructions and DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (Rat bladder);Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 ? for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4 ? ,followed by operating according to SP Kit(Rabbit) instructions and DAB staining.)
Related Product Information for anti-Pan Cytokeratin antibody
Description: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas.
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Product Notes
The Pan Cytokeratin (Catalog #AAA269011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pan CytokeRatin reacts with Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Pan Cytokeratin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Pan Cytokeratin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pan Cytokeratin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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