WB (Western Blot)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Mouse HIF1 alpha Monoclonal Antibody | anti-Hif1a antibody
HIF1 alpha Antibody: FITC
Gene Names
Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha
Reactivity
Human, Mouse, Rat, Cow
Applications
Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Synonyms
HIF1 alpha, Antibody; HIF1 alpha Antibody: FITC; ARNT interacting protein; HIF1A; HIF-1 alpha; Hypoxia inducible factor 1 alpha; MOP1; PASD8; anti-Hif1a antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Cow
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ESEE122
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
836
Applicable Applications for anti-Hif1a antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Conjugate
FITC
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
WB (Western Blot)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
WB (Western Blot)
(Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.)
Related Product Information for anti-Hif1a antibody
Background Info: Specific for HIF1Alpha
Scientific Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
Scientific Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
Product Categories/Family for anti-Hif1a antibody
References
1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5, 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. 3. Jaakkola P., et al. (2001) Science 292, 468-72. 4. Maxwell P.H., et al. (1999) Nature 399, 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277, 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12, 363- 9. 7. Laughner E., et al. (2001) Mol Cell Biol 21, 3995- 4004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,874 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit
NCBI Official Symbol
Hif1a
NCBI Official Synonym Symbols
MOP1; bHLHe78; AA959795; HIF1alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha; HIF1-alpha; HIF-1-alpha; ARNT-interacting protein
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
Hif1a
UniProt Synonym Gene Names
HIF-1-alpha; HIF1-alpha
UniProt Entry Name
HIF1A_MOUSE
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Product Notes
The Hif1a hif1a (Catalog #AAA103477) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF1 alpha Antibody: FITC reacts with Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1 alpha can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Hif1a hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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