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IF (Immunofluorescence)
(NT2 cells were pretreated with 2N HCL for 30 minutes, washed 3x in PBS and stained for 2 hours with 1ug/50ul antibody. They were incubated in Alexa goat-rabbit 594 for 1 hour. PCNA: Red; DAPI: Blue)
Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody
PCNA antibody
Gene Names
PCNA; ATLD2
Applications
Western Blot, Immunohistochemistry
Synonyms
PCNA, Antibody; PCNA antibody; Polyclonal PCNA; Anti-PCNA; proliferating cell nuclear antigen; MGC8367; anti-PCNA antibody
Host
Rabbit
Clonality
Polyclonal
Form/Format
Lyophilized powder. Add 100 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration
1 mg/ml (varies by lot)
Sequence Length
261
Applicable Applications for anti-PCNA antibody
WB (Western Blot), IHC (Immunohistochemistry)
Biological Significance
PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Cross-Reactivity
Human, Mouse, Rat, Dog, Bovine, African Clawed Frog, Zebrafish
Immunogen
PCNA antibody was raised in rabbit using the C terminal of PCNA as the immunogen
Preparation and Storage
Store at 4 degree C, following reconstitution, aliquot and store at -20 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(NT2 cells were pretreated with 2N HCL for 30 minutes, washed 3x in PBS and stained for 2 hours with 1ug/50ul antibody. They were incubated in Alexa goat-rabbit 594 for 1 hour. PCNA: Red; DAPI: Blue)
IF (Immunofluorescence)
(NT2 cells were pretreated with 2N HCL for 30 minutes, washed 3x in PBS and stained for 2 hours with 1ug/50ul antibody. They were incubated in Alexa goat-rabbit 594 for 1 hour. PCNA: Red; DAPI: Blue)
WB (Western Blot)
(Sample Type: 1. Human NT-2 cells (60ug) PCNA antibody dilution: 2ug/ml)
WB (Western Blot)
(Sample Type: 1. Human NT-2 cells (60ug) PCNA antibody dilution: 2ug/ml)
IHC (Immunohiostchemistry)
(Paraffin Embedded Tissue: Human Spleen Cellular Data: Spleen cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X)
IHC (Immunohiostchemistry)
(Paraffin Embedded Tissue: Human Spleen Cellular Data: Spleen cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X)
WB (Western Blot)
(Recommended PCNA Antibody Titration: 1.25ug/ml)
WB (Western Blot)
(Recommended PCNA Antibody Titration: 1.25ug/ml)
Related Product Information for anti-PCNA antibody
Rabbit polyclonal PCNA antibody
Product Categories/Family for anti-PCNA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,769 Da
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Official Synonym Symbols
ATLD2
NCBI Protein Information
proliferating cell nuclear antigen
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
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Product Notes
The PCNA pcna (Catalog #AAA107499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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