WB (Western Blot)
(Detection of human PD-L1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, HeLa, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
PD-L1 recombinant antibody
Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR227K]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
PD-L1, Recombinant Antibody; Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR227K]; PDCD1L1; Programmed death ligand 1; programmed cell death 1 ligand 1; PD-L1; PDL1; PDCD1LG1; PDCD1 ligand 1; CD274 antigen; CD antigen CD274; B7-H1; B7H1; B7 homolog 1; B7-H; Programmed cell death 1 ligand 1; CD274 molecule; PD-L1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR227K
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for PD-L1 recombinant antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 19-238 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PD-L1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, HeLa, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human PD-L1 by western blot. Samples: Whole cell lysate (25 ug) from RKO, HeLa, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human PD-L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649 lot 1) used for IP at 20 ul/mg lysate. PD-L1 was also immunoprecipitated by a second antibody against a different epitope of PD-L1 . For blotting immunoprecipitated PD-L1, AAA213649 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PD-L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649 lot 1) used for IP at 20 ul/mg lysate. PD-L1 was also immunoprecipitated by a second antibody against a different epitope of PD-L1 . For blotting immunoprecipitated PD-L1, AAA213649 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of human PD-L1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human PD-L1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody (AAA213649) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for PD-L1 recombinant antibody
PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].
Product Categories/Family for PD-L1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,246 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform a
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The PD-L1 cd274 (Catalog #AAA213649) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR227K] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PD-L1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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