Rabbit anti-Human RNA pol II CTD Cit1810 Polyclonal Antibody | anti-Cit1810 antibody

RNA pol II CTD Cit1810 antibody (pAb)

Cat. Num. AAA60352

• Gene Names: POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Dot Blot
• Purity: Affinity Purified
• Synonyms: RNA pol II CTD Cit1810, Antibody; RNA pol II CTD Cit1810 antibody (pAb); RNA pol II CTD Cit1810 pAb; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-Cit1810 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in 70 mM Tris, 32 mM NaOAC, 0.035% sodium azide, 30% glycerol and 0.5 mg/mL BSA. Sodium azide is highly toxic.

• Applicable Applications for anti-Cit1810 antibody: WB (Western Blot), IP (Immunoprecipitation), DB (Dot Blot)
• Abs residue: Arg
• Modification: Citrullinated
• Immunogen: This RNA pol II CTD Cit 1810 antibody was raised against a peptide containing the RNA Pol II CTD sequence containing citrulline and corresponding to human RNA pol ll.
• Preparation and Storage: Store at -20 degree C. Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.

Application Data

Application Data

Application Data

Related Product Information for anti-Cit1810 antibody

RNA pol II CTD Cit1810 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. This antibody is specific for the deamination of arginine and its conversation to citrulline by peptidyl arginine deiminase 2 (PADI2), an enzyme that has been associated with several diseases, including cancer. Among the PADI family members, only PADI2 citrullinates R1810 (Cit1810) at repeat 31 of the CTD. Loss of R1810 result in increased amounts of RNAP2 at transcription start cites, reduce gene expression, and inhibits cell proliferation.

Product Categories/Family for anti-Cit1810 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4505939
• NCBI GeneID: 5430
• NCBI Accession #: NP_000928
• NCBI GenBank Nucleotide #: NM_000937
• UniProt Accession #: P24928; Q6NX41; A6NN93; B9EH88
• Molecular Weight: 63,641 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB1
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• NCBI Official Symbol: POLR2A
• NCBI Official Synonym Symbols: RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB1
• UniProt Gene Name: POLR2A
• UniProt Synonym Gene Names: POLR2; RNA polymerase II subunit B1
• UniProt Entry Name: RPB1_HUMAN