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ChIP (Chromatin Immunoprecipitation)
(AbFlex RNA Pol II CTD phospho Tyr1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of Raji cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 17.3 million sequence tags were mapped to identify RNA Pol II CTD phosphor Tyr1 binding sites on chromosome 12.)
Mouse anti-Human RNA Pol II CTD phospho Tyr1 Antibody | anti-Tyr1 antibody
AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
RNA Pol II CTD phospho Tyr1; N/A; AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb); RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-Tyr1 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Tyr1 antibody
WB (Western Blot), IF (Immunofluorescence), ELISA, ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Conjugation
Phosphorylated
Immunogen
This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
ChIP (Chromatin Immunoprecipitation)
(AbFlex RNA Pol II CTD phospho Tyr1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of Raji cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 17.3 million sequence tags were mapped to identify RNA Pol II CTD phosphor Tyr1 binding sites on chromosome 12.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex RNA Pol II CTD phospho Tyr1 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of Raji cell chromatin and 10 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 17.3 million sequence tags were mapped to identify RNA Pol II CTD phosphor Tyr1 binding sites on chromosome 12.)
IF (Immunofluorescence)
(AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Immunofluorescence. HeLa cells were stained with 2 ug /mL of AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) followed by anti-mouse IgG-488.)
IF (Immunofluorescence)
(AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) tested by Immunofluorescence. HeLa cells were stained with 2 ug /mL of AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) followed by anti-mouse IgG-488.)
Application Data
(AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) antibody for RNA pol II CTD peptides. RNA pol II peptides were conjugated to MagPlex Luminex beads and incubated with 500 ng of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin (PE) and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
Application Data
(AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb) antibody for RNA pol II CTD peptides. RNA pol II peptides were conjugated to MagPlex Luminex beads and incubated with 500 ng of AbFlex RNA pol II CTD phospho Tyr1 antibody (rAb). Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin (PE) and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.)
SDS-PAGE
(AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) (rAb) tested by Western Blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex RNA Pol II CTD phospho Tyr1 antibody at 2 ug/ml.)
SDS-PAGE
(AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) (rAb) tested by Western Blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex RNA Pol II CTD phospho Tyr1 antibody at 2 ug/ml.)
Related Product Information for anti-Tyr1 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) was raised in a Mouse host. It has been validated for use in ChIP-Seq, ELISA, Immunofluorescence and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Product Categories/Family for anti-Tyr1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,641 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The Tyr1 polr2a (Catalog #AAA60276) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNA Pol II CTD phospho Tyr1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ELISA, ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Tyr1 polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA Pol II CTD phospho Tyr1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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