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WB (Western Blot)
(RNA pol II CTD phospho Ser5 mAb tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
Rat anti-Human RNA pol II CTD phospho Ser5 Monoclonal Antibody | anti-Ser5 antibody
RNA pol II CTD phospho Ser5 antibody (mAb)(Clone 3E8)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
RNA pol II CTD phospho Ser5, Antibody; RNA pol II CTD phospho Ser5 antibody (mAb)(Clone 3E8); RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-Ser5 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3E8
Purity/Purification
Protein G Chromatography
Form/Format
Supplied in PBS containing 0.05% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Ser5 antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(RNA pol II CTD phospho Ser5 mAb tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
WB (Western Blot)
(RNA pol II CTD phospho Ser5 mAb tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with antibody at 2 ug/ml.)
IF (Immunofluorescence)
(Detection of RNA Pol II pS5 by immunofluorescence U2OS cells were stained with RNA Pol II pS5 antibody at a dilution of 1:500. Left panel: RNA Pol II pS5 antibody staining. Middle panel:DAPI. Right panel: merge.)
IF (Immunofluorescence)
(Detection of RNA Pol II pS5 by immunofluorescence U2OS cells were stained with RNA Pol II pS5 antibody at a dilution of 1:500. Left panel: RNA Pol II pS5 antibody staining. Middle panel:DAPI. Right panel: merge.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP-Seq.ChIP-Seq was performed in a bladder cancer cell line using RNA pol II CTD phospho Ser5 antibody (Cat. No. AAA59975) and RNA pol II CTD phospho Ser2 antibody (Cat. No. The average ChIP-Seq signal across all genes is shown in the graphic. As expected Pol II phosphoserine 5 is enriched at promoters and phosphoserine 2 is enriched toward the 3’ end of genes.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP-Seq.ChIP-Seq was performed in a bladder cancer cell line using RNA pol II CTD phospho Ser5 antibody (Cat. No. AAA59975) and RNA pol II CTD phospho Ser2 antibody (Cat. No. The average ChIP-Seq signal across all genes is shown in the graphic. As expected Pol II phosphoserine 5 is enriched at promoters and phosphoserine 2 is enriched toward the 3’ end of genes.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP-Seq.ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. please inquire) with chromatin from 2.3 million HL60 cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 23 million sequence tags were mapped to identify RNA pol II phospho Ser5 binding. Data is compared to ChIP-Seq data using a phospho Ser2 antibody . ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser5 antibody detects polymerase more at the 5´ end of the genes and the phospho Ser2 antibody detects Pol II more toward the 3´ end of the genes.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP-Seq.ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. please inquire) with chromatin from 2.3 million HL60 cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 23 million sequence tags were mapped to identify RNA pol II phospho Ser5 binding. Data is compared to ChIP-Seq data using a phospho Ser2 antibody . ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser5 antibody detects polymerase more at the 5´ end of the genes and the phospho Ser2 antibody detects Pol II more toward the 3´ end of the genes.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. please inquire) with 10 ug of chromatin from human myeloma LP1 cells and 10 ug RNA pol II CTD phospho Ser5 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP.Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. please inquire) with 10 ug of chromatin from human myeloma LP1 cells and 10 ug RNA pol II CTD phospho Ser5 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Related Product Information for anti-Ser5 antibody
Background: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
Product Categories/Family for anti-Ser5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
240 kDa
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The Ser5 polr2a (Catalog #AAA59975) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RNA pol II CTD phospho Ser5 antibody (mAb)(Clone 3E8) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNA pol II CTD phospho Ser5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Ser5 polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA pol II CTD phospho Ser5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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