Mouse anti-Human RNA Pol II Antibody | anti-RNAPolII antibody

AbFlex RNA Pol II antibody (rAb)

Cat. Num. AAA60265

• Gene Names: POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Protein A
• Synonyms: RNA Pol II; N/A; AbFlex RNA Pol II antibody (rAb); RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-RNAPolII antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Purity/Purification: Protein A
• Form/Format: Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-RNAPolII antibody: WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
• Antibody Type: Recombinant Antibody
• Immunogen: This RNA Pol II antibody was raised against a synthetic peptide YSPTSpPS corresponding to human RNA pol II.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

IF (Immunofluorescence)

(Immunofluorescence stain of AbFlex RNA Pol II antibody (rAb). RNA Pol II was detected in human HeLa cells in a 2-step staining protocol using AbFlex RNA Pol II (rAb) at 2 ug/mL, followed by Anti-mouse IgG2a Dylight 488.)

WB (Western Blot)

(Western Blot of AbFlex RNA Pol II antibody (rAb). Western Blot: 30 ug HeLa cell nuclear extract* was run on an SDS-PAGE gel and probed with AbFlex RNA Pol II (rAb) at 1 ug/mL. *Nuclear extract prepared with high salt / sonication protocol. Contact Technical Service for additional info. MW: 217 kDa)

ChIP (Chromatin Immunoprecipitation)

(ChIP-Seq of AbFlex RNA Pol II antibody (rAb). ChIP was performed using AbFlex RNA Pol II recombinant antibody with 20 ug chromatin from the HT1080 cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify RNA Pol II binding sites. The image shows binding across a region of chromosome 12.)

Related Product Information for anti-RNAPolII antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex RNA Pol II antibody (rAb) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunofluorescence and Western blot; it has been shown to react with Human samples.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex RNA Pol II antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Product Categories/Family for anti-RNAPolII antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4505939
• NCBI GeneID: 5430
• NCBI Accession #: NP_000928; AB_2793789
• NCBI GenBank Nucleotide #: NM_000937
• UniProt Accession #: P24928; Q6NX41; A6NN93; B9EH88
• Molecular Weight: 63,641 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB1
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• NCBI Official Symbol: POLR2A
• NCBI Official Synonym Symbols: RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB1
• UniProt Gene Name: POLR2A
• UniProt Synonym Gene Names: POLR2; RNA polymerase II subunit B1
• UniProt Entry Name: RPB1_HUMAN