DB (Dot Blot)
(Dot blot analysis was used to confirm the specificity of RNA Pol II phospho Thr4 antibody 10, 50 and 250 pmoles of RNA Pol II CTD were spotted onto nitrocellulose and probed with the antibody at 1 ug/ml. Lane 1: RNA Pol II CTD phospho-Thr4. Lane 2: RNA Pol II CTD Thr4 (unmodified))
Rat RNA pol II CTD phospho Thr4 Monoclonal Antibody | anti-Thr4 antibody
RNA pol II CTD phospho Thr4 antibody (mAb)(Clone 6D7)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Wide Range Predicted
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
RNA pol II CTD phospho Thr4, Antibody; RNA pol II CTD phospho Thr4 antibody (mAb)(Clone 6D7); RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; anti-Thr4 antibody
Host
Rat
Reactivity
Human, Wide Range Predicted
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6D7
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Thr4 antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, DB (Dot Blot), ChIP (Chromatin immunoprecipitation)
Conjugation
Phosphorylated
Immunogen
This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.
Abs Residue
Thr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(Dot blot analysis was used to confirm the specificity of RNA Pol II phospho Thr4 antibody 10, 50 and 250 pmoles of RNA Pol II CTD were spotted onto nitrocellulose and probed with the antibody at 1 ug/ml. Lane 1: RNA Pol II CTD phospho-Thr4. Lane 2: RNA Pol II CTD Thr4 (unmodified))
DB (Dot Blot)
(Dot blot analysis was used to confirm the specificity of RNA Pol II phospho Thr4 antibody 10, 50 and 250 pmoles of RNA Pol II CTD were spotted onto nitrocellulose and probed with the antibody at 1 ug/ml. Lane 1: RNA Pol II CTD phospho-Thr4. Lane 2: RNA Pol II CTD Thr4 (unmodified))
Application Data
(RNA pol II CTD phospho Thr4 antibody tested by bead-based assay Luminex bead-based specificity analysis was used to confirm the specificity of RNA pol II CTD phospho Thr4 antibody for RNA pol II CTD peptides conjugated to MagPlex Luminex beads and incubated with 50ng of RNA pol II CTD phospho Thr4 antibody. Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex is a registered trademark of Luminex Corporation.))
Application Data
(RNA pol II CTD phospho Thr4 antibody tested by bead-based assay Luminex bead-based specificity analysis was used to confirm the specificity of RNA pol II CTD phospho Thr4 antibody for RNA pol II CTD peptides conjugated to MagPlex Luminex beads and incubated with 50ng of RNA pol II CTD phospho Thr4 antibody. Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex is a registered trademark of Luminex Corporation.))
WB (Western Blot)
(Western blot of RNA pol II CTD phospho Thr4 mAb. 30 ug of HeLa cell nuclear extract was probed with RNA pol II CTD phospho Thr4 antibody at 2 ug/ml in buffer containing 0.05% Tween.)
WB (Western Blot)
(Western blot of RNA pol II CTD phospho Thr4 mAb. 30 ug of HeLa cell nuclear extract was probed with RNA pol II CTD phospho Thr4 antibody at 2 ug/ml in buffer containing 0.05% Tween.)
Related Product Information for anti-Thr4 antibody
Short Description: Our RNA pol II CTD phospho Thr4 antibody (mAb)(Clone 6D7) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.
Background: RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.
Product Categories/Family for anti-Thr4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,641 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The Thr4 polr2a (Catalog #AAA60012) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RNA pol II CTD phospho Thr4 antibody (mAb)(Clone 6D7) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's RNA pol II CTD phospho Thr4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, DB (Dot Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Thr4 polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA pol II CTD phospho Thr4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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