Mouse anti-Human TARDBP Monoclonal Antibody | anti-TARDBP antibody

TARDBP antibody

Cat. Num. AAA48023

• Gene Names: TARDBP; ALS10; TDP-43
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: By protein-G affinity chromatography
• Synonyms: TARDBP, Antibody; TARDBP antibody; TAR DNA binding protein; TDP-43; ALS10; TAR DNA-binding protein 43; TDP43; anti-TARDBP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: k1B8
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 414

• Applicable Applications for anti-TARDBP antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Antigen Species: Human
• Immunogen: Recombinant human TARDBP (1-260aa) purified from E. coli
• Clone Note: Anti-human TARDBP mAb, clone k1B8, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TARDBP protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

IHC (Immunohiostchemistry)

(Paraffin embedded sections of human prostate cancer and seminoma tissue were incubated with anti-human TARDBP (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

WB (Western Blot)

(Cell lysates of HeLa (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TARDBP (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-TARDBP antibody

TAR DNA binding protein (TARDBP), also known as TDP-43, is a cellular protein. TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS). TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.

Product Categories/Family for anti-TARDBP antibody

Transcription & Translation

References

Nicola JR, et al., (2008) PLoS Genet 4 (9):e1000193; Kabashi E, et al., (2008) Nat Genet 40 (5):572-574 Ou SH, et al., (1995) J Virol 69:3584-3596

NCBI and Uniprot Product Information

• NCBI GI #: 6678271
• NCBI GeneID: 23435
• NCBI Accession #: NP_031401
• NCBI GenBank Nucleotide #: NM_007375.3
• UniProt Accession #: Q13148; Q53H27; Q6FI92; Q96DJ0; A4GUK4; A4GUK5; A4GUK6; B2R629; B4DJ45; E2PU12
• Molecular Weight: 31,808 Da
• NCBI Official Full Name: TAR DNA-binding protein 43
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: TARDBP
• NCBI Official Synonym Symbols: ALS10; TDP-43
• NCBI Protein Information: TAR DNA-binding protein 43
• UniProt Protein Name: TAR DNA-binding protein 43
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43