Mouse anti-Human CD329 Antibody | anti-CD329 antibody

Anti-Hu CD329 APC

Cat. Num. AAA129362

• Gene Names: SIGLEC9; CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Synonyms: CD329; N/A; Anti-Hu CD329 APC; sialic acid binding Ig like lectin 9; SIGLEC9; anti-CD329 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Clone Number: K8
• Specificity: The mouse monoclonal antibody K8 recognizes an extracellular epitope of CD239, a type I transmembrane glycoprotein expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells.
• Purity/Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Applicable Applications for anti-CD329 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: recombinant CD329-Hu IgGFc fusion protein
• Conjugation: APC
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human monocytes (red-filled) from CD329 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD329 (K8) APC antibody (10ul reagent/100ul of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD329 (K8) APC antibody (10ul reagent/100ul of peripheral whole blood).)

Related Product Information for anti-CD329 antibody

CD329 is a type I transmembrane glycoprotein of siglec family, which contains intracellular ITIM motif, and a SLAM-like motif, that acts as a docking site for SAP. CD329 can regulate TCR signaling by recruitment of SHP-1, which results in down-regulation of TCR-based gene transcription. It is expressed above all on monocytes, neutrophils, and a minor population of CD16+CD56- cells, weaker expression is detectable in some B cells, NK cells, and T cells.

References

*O'Sullivan JA, Carroll DJ, Cao Y, Salicru AN, Bochner BS: Leveraging Siglec-8 endocytic mechanisms to kill human eosinophils and malignant mast cells. J Allergy Clin Immunol. 2018 May;141(5):1774-1785, URL: https://pubmed.ncbi.nlm.nih.gov/28734845/,*Hudak JE, Canham SM, Bertozzi CR: Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion. Nat Chem Biol. 2014 Jan;10(1):69-75., URL: https://pubmed.ncbi.nlm.nih.gov/24292068/,*Avril T, Floyd H, Lopez F, Vivier E, Crocker PR: The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells. J Immunol. 2004 Dec 1;173(11):6841-9. , URL: https://pubmed.ncbi.nlm.nih.gov/15557178/,

NCBI and Uniprot Product Information

• NCBI GI #: 25009270
• NCBI GeneID: 27180
• UniProt Accession #: Q9Y336; Q6GTU4; Q9BYI9
• Molecular Weight: 52,492 Da
• NCBI Official Full Name: Sialic acid-binding Ig-like lectin 9
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 9
• NCBI Official Symbol: SIGLEC9
• NCBI Official Synonym Symbols: CD329; CDw329; FOAP-9; siglec-9; OBBP-LIKE
• NCBI Protein Information: sialic acid-binding Ig-like lectin 9
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 9
• UniProt Gene Name: SIGLEC9
• UniProt Synonym Gene Names: Siglec-9