IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Mouse SIRT1 Antibody | anti-SIRT1 antibody
SIRT1 Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human, Monkey
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
SIRT1; N/A; SIRT1 Antibody; SIR2L1; anti-SIRT1 antibody
Host
Mouse
Reactivity
Human, Monkey
Isotype
IgG1
Clone Number
1F3
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
452
Applicable Applications for anti-SIRT1 antibody
ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human SIRT1 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C.
IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Figure 3: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.)
IHC (Immunohiostchemistry)
(Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.)
WB (Western Blot)
(Figure 1: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.)
WB (Western Blot)
(Figure 1: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.)
Related Product Information for anti-SIRT1 antibody
The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta.
Product Categories/Family for anti-SIRT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120kDa
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform b
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2; SIR2L1; SIR2alpha
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1
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Product Notes
The SIRT1 sirt1 (Catalog #AAA108726) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SIRT1 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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