SDS-PAGE
(The purity of PCNA was determined to be >95%by densitometry. Approx. MW 37kDa)
PCNA recombinant protein
PCNA recombinant protein
Applications
Western Blot
Purity
>95%
Synonyms
PCNA; N/A; PCNA recombinant protein; MGC8367
Host
E Coli
Purity/Purification
>95%
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.2 ug/ul (varies by lot)
Sequence Length
1355
Applicable Applications for PCNA recombinant protein
WB (Western Blot)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
E.coli
Source Note
Recombinant full-length human PCNA was expressed by E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on Dry Ice.
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(The purity of PCNA was determined to be >95%by densitometry. Approx. MW 37kDa)
SDS-PAGE
(The purity of PCNA was determined to be >95%by densitometry. Approx. MW 37kDa)
Related Product Information for PCNA recombinant protein
Recombinant full-length human PCNA was expressed by E. coli cells using an N-terminal His tag.
Product Categories/Family for PCNA recombinant protein
References
1.Hasan, S. et al: Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis. Nature 410: 387-391, 2001.
2.Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
Storage, Stability and Shipping: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Western Blot
Molecular Weight: ~37 kDa
Gene Aliases: MGC8367
Scientific Background: PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro (1). PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H (2).
2.Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
Storage, Stability and Shipping: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Western Blot
Molecular Weight: ~37 kDa
Gene Aliases: MGC8367
Scientific Background: PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro (1). PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H (2).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37 kDa
NCBI Official Full Name
Homo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1, mRNA
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Protein Information
proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
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Product Notes
The PCNA pcna (Catalog #AAA72901) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His tag!!Expression System||E.coli!!Source Note||Recombinant full-length human PCNA was expressed by E. coli cells. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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