Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using NLK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using NLK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using NLK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using NLK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using NLK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spinal cord using NLK antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NLK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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NLK, Polyclonal Antibody (Cat# AAA10731)

Full Name

NLK Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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UCH-L1, Polyclonal Antibody (Cat# AAA29154)

Full Name

UCH-L1 Polyclonal Antibody

Gene Names

UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat lung tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse lung tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.)

WB (Western Blot) (Figure 1. Western blot analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: human 22RV1 whole cell lysates,Lane 7: human A431 whole cell lysates,Lane 8: human COLO-320 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE2/UBA2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SAE2/UBA2 at approximately 90KD. The expected band size for SAE2/UBA2 is at 71KD.)

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SAE2/UBA2, Polyclonal Antibody (Cat# AAA19171)

Full Name

Anti-SAE2/UBA2 Picoband antibody

Gene Names

UBA2; ARX; SAE2; HRIHFB2115

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat fetal liver using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse fetal liver using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain using Survivin Polyclonal Antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using Survivin Polyclonal Antibody at 1:500 dilution.)

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Survivin, Polyclonal Antibody (Cat# AAA22246)

Full Name

Survivin Polyclonal Antibody

Gene Names

BIRC5; API4; EPR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FCM (Flow Cytometry) (Flow Cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Application Data (IC staining of Hela cells)

IHC (Immunohistochemistry) (Immunohistochemical staining of formalin-fixed and paraffin-embedded human cancer tissue)

WB (Western Blot) (Western Blot analysis of Hela cell line lysate (35ug/lane) using antibody.)

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FGFR2, Polyclonal Antibody (Cat# AAA26854)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (FITC)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

FLISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS),

Purity

Purified by Protein G Affinity Chromatography.

Pricing

$1,055/0.2 mL $2,045/2x0.2 mL $2,980/3x0.2 mL $3,865/4x0.2 mL $4,515/5x0.2 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug TP53 antibody. Western blot was performed from the immunoprecipitate using TP53 antibody at a dilition of 1:500.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using TP53 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TP53 antibody at 1:6000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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TP53, Polyclonal Antibody (Cat# AAA28134)

Full Name

TP53 Polyclonal Antibody

Gene Names

Trp53; bbl; bfy; bhy; p44; p53; Tp53

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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KCNN2, Polyclonal Antibody (Cat# AAA28902)

Full Name

KCNN2 (SK2) Polyclonal Antibody

Gene Names

KCNN2; SK2; hSK2; SKCA2; KCa2.2; SKCa 2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Host: RabbitTarget Name: FAM46CSample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: EGFL8Sample Type: HelaAntibody Dilution: 1.0ug/mlCYP11A1 is supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (CYP11A1 antibody - middle region validated by WB using Hela cell lysate at 1ug/ml.CYP11A1 is supported by BioGPS gene expression data to be expressed in HeLa)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Placenta lysate tissue at an antibody concentration of 5.0ug/ml using anti-CYP11A1 antibody)

IHC (Immunohistochemistry) (Immunohistochemistry with HK2 cell lysate tissue)

IHC (Immunohistochemistry) (Immunohistochemistry with HK2 cell lysate tissue)

IHC (Immunohistochemistry) (Immunohistochemistry with HK2 cell lysate tissue)

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CYP11A1, Polyclonal Antibody (Cat# AAA23543)

Full Name

CYP11A1 antibody - middle region

Gene Names

CYP11A1; CYP11A; CYPXIA1; P450SCC

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in K-562.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in Jurkat.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse testis.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse spleen.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human pancreas.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human liver.)

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FKBP4, Polyclonal Antibody (Cat# AAA26871)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (PE)

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,035/0.1 mL $2,005/2x0.1 mL $2,925/3x0.1 mL $3,790/4x0.1 mL $4,420/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

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Akt, Polyclonal Antibody (Cat# AAA28919)

Full Name

Akt (phospho Ser473) Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CyPA, Polyclonal Antibody (Cat# AAA29175)

Full Name

CyPA Polyclonal Antibody

Gene Names

PPIA; CYPA; CYPH

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/50.)

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S100B, Polyclonal Antibody (Cat# AAA29687)

Full Name

S100B Antibody

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human Mouse Rat

Applications

Immunohistochemistry (IHC)

Purity

Antigen affinity purification.

Pricing

$335/0.1 mL $640/2x0.1 mL $930/3x0.1 mL $1,200/4x0.1 mL $1,285/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using DDX50 at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDX50 at 1:3000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using DDX50 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using DDX50 at dilution of 1:100 (40x lens).)

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DDX50, Polyclonal Antibody (Cat# AAA30711)

Full Name

DDX50 Rabbit Polyclonal Antibody

Gene Names

DDX50; GU2; GUB; mcdrh; RH-II/GuB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

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AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26873)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (APC)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,115/0.1 mL $2,160/2x0.1 mL $3,150/3x0.1 mL $4,085/4x0.1 mL $4,790/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Jurkat, T47D cell line (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

FCM (Flow Cytometry) (Flow cytometric analysis of HeLa cells using EZH2 Antibody (green) compared to an isotype control of rabbit IgG(blue). AAA28665 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

WB (Western Blot) (All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MDA-MB-231 whole cell lysatesLane 3: MDA-MB-468 whole cell lysatesLane 4: T47D whole cell lysatesLane 5: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: mouse testis lysatesLane 2: MDA-MB-231 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-EZH2 Antibodyat 1:500 dilution + MDA-MB-468 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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EZH2, Polyclonal Antibody (Cat# AAA28665)

Full Name

EZH2 Antibody

Gene Names

EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using ASB3 Polyclonal Antibody at 1:1000 dilution.)

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ASB3, Polyclonal Antibody (Cat# AAA22266)

Full Name

ASB3 Polyclonal Antibody

Gene Names

ASB3; ASB-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-PDIA6 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HepG2 cell lysate.PDIA6 is strongly supported by BioGPS gene expression data to be expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: PDIA6Sample Type: MCF7Antibody Dilution: 1.0ug/mlPDIA6 is strongly supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: PDIA6Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PDIA6Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PDIA6Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PDIA6Sample Type: 721_BAntibody Dilution: 1.0ug/mlPDIA6 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

IHC (Immunohistochemistry) (Immunohistochemistry with Small intestine tissue)

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PDIA6, Polyclonal Antibody (Cat# AAA23546)

Full Name

PDIA6 antibody - middle region

Gene Names

PDIA6; P5; ERP5; TXNDC7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Raptor Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Raptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Raptor Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of 293T cells using Raptor Polyclonal Antibody at 1:1000 dilution.)

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Raptor, Polyclonal Antibody (Cat# AAA22260)

Full Name

Raptor Polyclonal Antibody

Gene Names

RPTOR; KOG1; Mip1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in K-562.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in Jurkat.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse testis.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in mouse spleen.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human pancreas.)

WB (Western Blot) (FKBP4 rabbit polyclonal antibody. Western Blot analysis of FKBP4 expression in human liver.)

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FKBP4, Polyclonal Antibody (Cat# AAA26868)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (Biot

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,035/0.1 mL $2,005/2x0.1 mL $2,925/3x0.1 mL $3,790/4x0.1 mL $4,420/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MAP2K5 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAP2K5 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)

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MAP2K5, Polyclonal Antibody (Cat# AAA28148)

Full Name

MAP2K5 Polyclonal Antibody

Gene Names

MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (LALBA Antibody (Center) (AAA28660)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (LALBA Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LALBA antibody detected the LALBA protein (arrow).)

WB (Western Blot) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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LALBA, Polyclonal Antibody (Cat# AAA28660)

Full Name

LALBA Antibody (Center)

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1, 000 dilution.)

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Fibulin 5, Polyclonal Antibody (Cat# AAA29940)

Full Name

Fibulin 5 Antibody

Gene Names

FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using PRDM2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using PRDM2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using PRDM2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using PRDM2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using PRDM2 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of rat heart, using PRDM2 at 1:1000 dilution.)

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PRDM2, Polyclonal Antibody (Cat# AAA30708)

Full Name

PRDM2 Rabbit Polyclonal Antibody

Gene Names

PRDM2; RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1

Reactivity

Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TOLLIP antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using TOLLIP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using TOLLIP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using TOLLIP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TOLLIP antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using TOLLIP antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TOLLIP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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TOLLIP, Polyclonal Antibody (Cat# AAA10741)

Full Name

TOLLIP Polyclonal Antibody

Gene Names

TOLLIP; IL-1RAcPIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

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CEACAM5, Polyclonal Antibody (Cat# AAA19192)

Full Name

Anti-CEACAM5 Antibody

Gene Names

CEACAM5; CEA; CD66e

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescent analysis of SLC39A8 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

WB (Western Blot) (Western blot analysis of SLC39A8 expression in HL60 (A) whole cell lysates. (Predicted band size: 43; 48; 49 kD; Observed band size: 50 kD))

WB (Western Blot) (Western blot analysis of SLC39A8 expression in HeLa (A), mouse liver (B) whole cell lysates. (Predicted band size: 43; 48; 49 kD; Observed band size: 50 kD))

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SLC39A8, Polyclonal Antibody (Cat# AAA27896)

Full Name

Anti-SLC39A8 Antibody

Gene Names

SLC39A8; ZIP8; PP3105; BIGM103; LZT-Hs6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified by Immunogen Affinity Chromatography

Pricing

$235/0.03 mL $255/0.05 mL $315/0.1 mL $405/0.2 mL $780/2x0.2 mL $1,130/3x0.2 mL $1,460/4x0.2 mL $1,575/5x0.2 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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Akt1, Polyclonal Antibody (Cat# AAA28920)

Full Name

Akt1 (phospho Thr450) Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.)

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CACNG4, Polyclonal Antibody (Cat# AAA29944)

Full Name

CACNG4 Antibody

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified.

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of ADAR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of ADAR1 expression in Jurkat (A), Ramos (B), HeLa (C) whole cell lysates.)

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ADAR1, Polyclonal Antibody (Cat# AAA17913)

Full Name

Anti-ADAR1 Antibody

Gene Names

ADAR; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP

Reactivity

Human, Mouse, Rat, Porcine

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$235/0.03 mL $255/0.05 mL $315/0.1 mL $405/0.2 mL $780/2x0.2 mL $1,130/3x0.2 mL $1,460/4x0.2 mL $1,575/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-PPP3CA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)

WB (Western Blot) (Host: RabbitTarget Name: PPP3CASample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PPP3CASample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PPP3CASample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PPP3CASample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: PPP3CASample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-PPP3CA antibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult Brain, cerebellumObserved Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

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PPP3CA, Polyclonal Antibody (Cat# AAA23542)

Full Name

PPP3CA antibody - middle region

Gene Names

PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; ACCIID; CALNA1; IECEE1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining ERGI3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ERGI3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERGI3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of ERGI3 on different lysates using anti-ERGI3 antibody at 1/500 dilution. Positive control�� Lane1: SiHa Lane2: Human placenta Lane3: Rat bone marrow)

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ERGI3, Polyclonal Antibody (Cat# AAA29942)

Full Name

ERGI3 Antibody

Gene Names

ERGIC3; Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified.

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AIFM3 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using AIFM3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AIFM3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using AIFM3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using AIFM3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using AIFM3 at dilution of 1:100 (40x lens).)

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AIFM3, Polyclonal Antibody (Cat# AAA30710)

Full Name

AIFM3 Rabbit Polyclonal Antibody

Gene Names

AIFM3; AIFL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human rectal cancer using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using CDK4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using CDK4 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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CDK4, Polyclonal Antibody (Cat# AAA10743)

Full Name

CDK4 Polyclonal Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (Anti-SLC10A1 antibody, AAA11511, Western blottingAll lanes: Anti SLC10A1 (AAA11511) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 38-56KDObserved bind size: 49KD)

IHC (Immunohistochemistry) (Anti-SLC10A1 antibody, AAA11511, IHC(P)IHC(P): Human Liver Cancer Tissue)

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SLC10A1, Polyclonal Antibody (Cat# AAA11511)

Full Name

Anti-SLC10A1 antibody

Gene Names

SLC10A1; NTCP

Reactivity

Human, Rat
Predict: Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Mouse brain using CDK19 Polyclonal Antibody at 1:500 dilution.)

WB (Western Blot) (Western blot analysis of extracts of DU145 cells using CDK19 Polyclonal Antibody at 1:1000 dilution.)

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CDK19, Polyclonal Antibody (Cat# AAA22259)

Full Name

CDK19 Polyclonal Antibody

Gene Names

CDK19; CDK11; CDC2L6; bA346C16.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-C19orf2 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Muscle)

WB (Western Blot) (Host: RabbitTarget Name: URI1Sample Type: MCF7Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that URI1 is expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: URI1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: URI1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: URI1Sample Type: HepG2Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that URI1 is expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: URI1Sample Type: HelaAntibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that URI1 is expressed in HeLa)

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C19orf2, Polyclonal Antibody (Cat# AAA23539)

Full Name

C19orf2 antibody - middle region

Gene Names

URI1; RMP; URI; NNX3; C19orf2; PPP1R19

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Cdc34, Polyclonal Antibody (Cat# AAA29171)

Full Name

Cdc34 Polyclonal Antibody

Gene Names

CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Hela cells using anti-PITPNB antibody (AAA19957).Overlay histogram showing Hela cells stained with AAA19957 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNB Antibody (AAA19957, 1ug/1x106 cells) for 30 min at 20 degree C. PE conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-PITPNB antibody (AAA19957).Overlay histogram showing Hela cells stained with AAA19957 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNB Antibody (AAA19957, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PITPNB using anti-PITPNB antibody (AAA19957).PITPNB was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PITPNB Antibody (AAA19957) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PITPNB using anti-PITPNB antibody (AAA19957).PITPNB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITPNB Antibody (AAA19957) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITPNB Antibody (AAA19957) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITPNB Antibody (AAA19957) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PITPNB Antibody (AAA19957) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat lung tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse lung tisue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITPNB antigen affinity purified polyclonal antibody (#AAA19957) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (35 kDa.)

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PITPNB, Polyclonal Antibody (Cat# AAA19957)

Full Name

Anti-PITPNB Antibody Picoband

Gene Names

PITPNB; VIB1B; PtdInsTP; PI-TP-beta

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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HoxD3, Polyclonal Antibody (Cat# AAA29173)

Full Name

HoxD3 Polyclonal Antibody

Gene Names

HOXD3; HOX4; HOX1D; HOX4A; Hox-4.1; MGC10470; HOXD3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using NRF2 Rabbit pAb at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using NRF2 Rabbit pAb at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

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NRF2, Polyclonal Antibody (Cat# AAA28410)

Full Name

NRF2 Rabbit pAb

Gene Names

LOC4339539; GPB-LR; RACK1B; OsJ_19467

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using PTPN6 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung using PTPN6 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain using PTPN6 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid cancer using PTPN6 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophageal cancer using PTPN6 antibody at dilution of 1:200 (400x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PTPN6 antibody.)

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PTPN6, Polyclonal Antibody (Cat# AAA29690)

Full Name

PTPN6 Antibody

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using BOK Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells using BOK Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using BOK Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using BOK Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using BOK Rabbit pAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BOK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

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BOK, Polyclonal Antibody (Cat# AAA28329)

Full Name

BOK Rabbit pAb

Gene Names

BOK; BOKL; BCL2L9

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

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PGD, Polyclonal Antibody (Cat# AAA29097)

Full Name

PGD Polyclonal Antibody

Gene Names

PGD; 6PGD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ADK antibody (AAA11690).Overlay histogram showing PC-3 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of U937 cells using anti-ADK antibody (AAA11690).Overlay histogram showing U937 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HL-60 cells using anti-ADK antibody (AAA11690).Overlay histogram showing HL-60 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ADK using anti- ADK antibody (AAA11690). ADK was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- ADK Antibody (AAA11690) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of ADK using anti- ADK antibody (AAA11690). ADK was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- ADK Antibody (AAA11690) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ADK using anti- ADK antibody (AAA11690). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- ADK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADK at approximately 40KD. The expected band size for ADK is at40KD.)

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ADK, Polyclonal Antibody (Cat# AAA11690)

Full Name

Anti-ADK Antibody

Gene Names

ADK; AK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

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PMS1, Polyclonal Antibody (Cat# AAA29101)

Full Name

PMS1 Polyclonal Antibody

Gene Names

PMS1; PMSL1; hPMS1; HNPCC3

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of rat brain, using GM130 at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GM130 at 1:1000 dilution.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GM130 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using GM130 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using GM130 at dilution of 1:200 (40x lens).)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of U-2 OS cells using GM130 Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using GM130 Polyclonal at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using GM130 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

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GM130, Polyclonal Antibody (Cat# AAA30637)

Full Name

GM130 Rabbit Polyclonal Antibody

Gene Names

GOLGA2; GM130

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of IL-17 in mouse thymus tissue with IL-17 Antibody at 20 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of IL-17 in A-20 cells with IL-17 antibody at 5 μg/ml.Green: IL-17 Antibody (4887)Blue: DAPI staining)

IHC (Immunohistochemistry) (Immunohistochemistry of IL-17 in mouse colon tissue with IL-17 antibody at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of IL-17 in human tonsil tissue with IL-17 antibody at 20 μg/ml.Green: IL-17 Antibody (4887)Blue: DAPI staining)

IHC (Immunohistochemistry) (Immunohistochemistry of IL-17 in human tonsil tissue with IL-17 antibody at 5 μg/ml.)

WB (Western Blot) (Western blot analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 μg/ml.)

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IL-17, Polyclonal Antibody (Cat# AAA10926)

Full Name

IL-17 Antibody

Gene Names

IL17A; IL17; CTLA8; IL-17; IL-17A

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

IL-17 Antibody is affinity chromatography purified via peptide column.

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CCDC6 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using CCDC6 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human appendix using CCDC6 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using CCDC6 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using CCDC6 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat lymph node using CCDC6 Polyclonal Antibody at dilution of 1:100 (40x lens).)

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CCDC6, Polyclonal Antibody (Cat# AAA22190)

Full Name

CCDC6 Polyclonal Antibody

Gene Names

CCDC6; H4; PTC; TPC; TST1; D10S170

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-GAPDH antibody Titration: 1 ug/mLSample Type: Human Raji)

WB (Western Blot) (WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain)

WB (Western Blot) (Lanes:1. 50 ug NIH3T3 cytosolic extract 2. 50 ug NIH3T3 cytosolic extract 3. 50 ug NIH3T3 cytosolic extract 4. 50 ug NIH3T3 cytosolic extractPrimary Antibody Dilution:1:1000Secondary Antibody:Anti-Rabbit HRPSecondary Antibody Dilution:1:30000Gene Name:GAPDHSubmitted by:Dr. Arimdam Basu, Dept. of Animal Biology, University of Pennsylvania)

WB (Western Blot) (Host: RabbitTarget Name: GAPDHSample Type: Hela Whole Cell lysatesAntibody Dilution: 1.0ug/mlGAPDH is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

WB (Western Blot) (Host: RabbitTarget Name: GAPDHSample Type: 293TLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)

IHC (Immunohistochemistry) (Rabbit Anti-GAPDH AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Liver TissueObserved Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (Rabbit Anti-GAPDH AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Heart Muscle TissueObserved Staining: CytoplasmPrimary Antibody Concentration: 1:600Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

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GAPDH, Polyclonal Antibody (Cat# AAA23468)

Full Name

GAPDH antibody - N-terminal region

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

IHC (Immunohistchemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

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Histone H3, Polyclonal Antibody (Cat# AAA28844)

Full Name

Histone H3 (Acetyl Lys9) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.