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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 800-850 of 3176 product results
IHC (Immunohistchemistry)
(Immunohistochemistry analysis of paraffin-embedded Rat brain using S100/S100A1 Rabbit pAb (AAA28617) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
S100/S100A1, Polyclonal Antibody (Cat# AAA28617)
Full Name
S100/S100A1 Rabbit pAb
Reactivity
Human
Applications
WB, IHC-P, EIA
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PTPIP51/RMDN3 antibody (AAA19923).Overlay histogram showing HepG2 cells stained with AAA19923 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PTPIP51/RMDN3, Polyclonal Antibody (Cat# AAA19923)
Full Name
Anti-PTPIP51/RMDN3 Antibody Picoband
Gene Names
RMDN3; RMD3; RMD-3; FAM82C; FAM82A2; ptpip51
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
ELISA
(Figure 1. Titration curves of anti-NP rabbit polyclonal antibody to SARS-CoV-2 NP protein. 96-well corning EILSA plate was coated with SARS-CoV-2 NP protein at a concentration of 2 ug/ml)
COVID 19 Nucleocapsid (NP) Coronavirus, Polyclonal Antibody (Cat# AAA13776)
Full Name
Anti-NP Protein (SARS-CoV-2) Polyclonal Antibody
Applications
WB, EIA
Purity
Protein G immunoaffinity chromatography
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of Thymidylate Synthase/TYMS using anti-Thymidylate Synthase/TYMS antibody (AAA19511).Thymidylate Synthase/TYMS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-Thymidylate Synthase/TYMS Antibody (AAA19511) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Thymidylate Synthase/TYMS, Polyclonal Antibody (Cat# AAA19511)
Full Name
Anti-Thymidylate Synthase/TYMS Antibody Picoband
Gene Names
TYMS; TS; TMS; HST422
Reactivity
Human, Monkey, Rat
Applications
EIA, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-ATG5 Antibody Titration: 1 ug/mlPositive Control: HepG2 cell lysate)
ATG5, Polyclonal Antibody (Cat# AAA23551)
Full Name
ATG5 antibody - C-terminal region
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Figure 11. IF analysis of H Cadherin/CDH13 using anti- H Cadherin/CDH13 antibody (AAA19252).H Cadherin/CDH13 was detected in immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- H Cadherin/CDH13 Antibody (AAA19252) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
H Cadherin/CDH13, Polyclonal Antibody (Cat# AAA19252)
Full Name
Anti-H Cadherin/CDH13 Antibody
Gene Names
CDH13; CDHH; P105
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300)
ERK 1/2, Polyclonal Antibody (Cat# AAA28947)
Full Name
ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
(Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). AAA28710 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
MICA, Polyclonal Antibody (Cat# AAA28710)
Full Name
MICA Antibody (Center)
Gene Names
MICA; MIC-A; PERB11.1
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
LIMK-1/2, Polyclonal Antibody (Cat# AAA29062)
Full Name
LIMK-1/2 Polyclonal Antibody
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using BAX antibody.)
BAX, Polyclonal Antibody (Cat# AAA29801)
Full Name
BAX Polyclonal Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p38 MAPK, Polyclonal Antibody (Cat# AAA31333)
Full Name
Phospho-p38 MAPK (Thr180/Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)
E-Selectin, Polyclonal Antibody (Cat# AAA29326)
Full Name
E-Selectin Polyclonal Antibody
Gene Names
SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
Reactivity
Human
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using COX15 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
COX15, Polyclonal Antibody (Cat# AAA30625)
Full Name
COX15 Rabbit Polyclonal Antibody
Gene Names
COX15; CEMCOX2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)
RUNX1, Polyclonal Antibody (Cat# AAA10677)
Full Name
RUNX1 Polyclonal Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, IP
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PKN2 (Ser815) in lysates of HeLa , using Phospho-PAK2 (Ser815) Antibody.)
PKN2, Polyclonal Antibody (Cat# AAA31159)
Full Name
Phospho-PKN2 (Ser815) Antibody
Gene Names
PKN2; PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using LRRK2 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
LRRK2, Polyclonal Antibody (Cat# AAA28347)
Full Name
LRRK2 Rabbit pAb
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
YAP, Polyclonal Antibody (Cat# AAA29139)
Full Name
YAP Polyclonal Antibody
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of C6 cells using anti-Clathrin heavy chain/CLTC antibody (AAA19270).Overlay histogram showing C6 cells stained with AAA19270 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Clathrin heavy chain/CLTC Antibody (AAA19270,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Clathrin heavy chain/CLTC, Polyclonal Antibody (Cat# AAA19270)
Full Name
Anti-Clathrin heavy chain/CLTC Antibody
Gene Names
CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8 Flow Cytometry analysis of RAW264.7 cells using anti-Integrin Linked ILK antibody (AAA19473).Overlay histogram showing RAW264.7 cells stained with AAA19473 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Linked ILK Antibody (AAA19473, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Integrin linked ILK, Polyclonal Antibody (Cat# AAA19473)
Full Name
Anti-Integrin linked ILK Antibody Picoband
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of JK cells using anti-SCP3/SYCP3 antibody (AAA19556).Overlay histogram showing JK cells stained with AAA19556 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCP3/SYCP3 Antibody (AAA19556, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SCP3/SYCP3, Polyclonal Antibody (Cat# AAA19556)
Full Name
Anti-SCP3/SYCP3 Antibody Picoband
Gene Names
SYCP3; COR1; SCP3; SPGF4
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-PVRL1/NECTIN1 antibody (AAA19303).Overlay histogram showing PC-3 cells stained with AAA19303 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PVRL1/NECTIN1 Antibody (AAA19303, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PVRL1/NECTIN1, Polyclonal Antibody (Cat# AAA19303)
Full Name
Anti-PVRL1/NECTIN1 Antibody
Gene Names
PVRL1; ED4; PRR; HIgR; HVEC; OFC7; PRR1; PVRR; CD111; PVRR1; SK-12; CLPED1; nectin-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells.)
POLK/DNA Polymerase Kappa, Polyclonal Antibody (Cat# AAA21279)
Full Name
Anti-POLK/DNA Polymerase Kappa Antibody
Gene Names
POLK; DINP; POLQ; DINB1
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
IF, IHC, IHC, WB
Purity
Affinity Purified
Pricing
Application Data
(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)
LGR5, Polyclonal Antibody (Cat# AAA17985)
Full Name
LGR5 Antibody
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, Mouse
Applications
WB, ICC
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500)
Cdk2/Cdc2, Polyclonal Antibody (Cat# AAA30744)
Full Name
Cdk2/Cdc2 (Phospho-Thr160) Rabbit Polyclonal Antibody
Gene Names
CDK2; p33(CDK2)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF, paraffin section, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse brain tissue,Mouse skeletal muscle tissue,ZebrafishAll lanes: Bmp4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)
Bone morphogenetic protein 4, Polyclonal Antibody (Cat# AAA26924)
Full Name
Rabbit anti-human Bone morphogenetic protein 4 polyclonal Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of K562 cells using anti-ITPA antibody (AAA19773).Overlay histogram showing K562 cells stained with AAA19773 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITPA Antibody (AAA19773, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ITPA, Polyclonal Antibody (Cat# AAA19773)
Full Name
Anti-ITPA Antibody Picoband
Gene Names
ITPA; My049; C20orf37; dJ794I6.3; HLC14-06-P
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
p53, Polyclonal Antibody (Cat# AAA29278)
Full Name
p53 Polyclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB, IHC-P
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
E2F-2, Polyclonal Antibody (Cat# AAA31340)
Full Name
E2F-2 Antibody
Gene Names
E2F2; E2F-2
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
hnRNP A2/B1, Polyclonal Antibody (Cat# AAA29040)
Full Name
hnRNP A2/B1 Polyclonal Antibody
Gene Names
HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; HNRPA2B1
Reactivity
Human, Mouse
Applications
WB, IHC-P, IF
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using HSPB1 antibody.)
HSPB1, Polyclonal Antibody (Cat# AAA29819)
Full Name
HSPB1 Polyclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of TLR3 expression in mouse spleen tissue lysate with TLR3 antibody at (A) 0.5 and (B) 1 μg/ml.)
TLR3, Polyclonal Antibody (Cat# AAA10938)
Full Name
TLR3 Antibody
Gene Names
TLR3; CD283; IIAE2
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF
Purity
TLR3 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human esophagus using GLUD1 at dilution of 1:100 (40x lens).)
GLUD1, Polyclonal Antibody (Cat# AAA30691)
Full Name
GLUD1 Rabbit Polyclonal Antibody
Gene Names
GLUD1; GDH; GDH1; GLUD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
ICC (Immunocytochemistry)
(ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
N-Cadherin, Polyclonal Antibody (Cat# AAA29917)
Full Name
N-Cadherin Antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of JK cells using anti-SH3GL2 antibody (AAA19548).Overlay histogram showing JK cells stained with AAA19548 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3GL2 Antibody (AAA19548, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SH3GL2, Polyclonal Antibody (Cat# AAA19548)
Full Name
Anti-SH3GL2 Antibody Picoband
Gene Names
SH3GL2; CNSA2; SH3P4; EEN-B1; SH3D2A
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-Kindlin 2/FERMT2 antibody (AAA19495).Overlay histogram showing MCF-7 cells stained with AAA19495 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kindlin 2/FERMT2 Antibody (AAA19495, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Kindlin 2/FERMT2, Polyclonal Antibody (Cat# AAA19495)
Full Name
Anti-Kindlin 2/FERMT2 Antibody Picoband
Gene Names
FERMT2; MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot;Sample: Recombinant APOB, Mouse.)
Apolipoprotein B (APOB), Polyclonal Antibody (Cat# AAA20169)
Full Name
Polyclonal Antibody to Apolipoprotein B (APOB)
Gene Names
Apob; apob-48; AI315052; apob-100; Apo B-100
Reactivity
Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] AKT1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
AKT1, Polyclonal Antibody (Cat# AAA22316)
Full Name
(KO Validated) AKT1 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using BMP5 antibody at dilution of 1:100 (40x lens).)
BMP5, Polyclonal Antibody (Cat# AAA28209)
Full Name
BMP5 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunochemical staining CLTC in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
CLTC, Polyclonal Antibody (Cat# AAA27734)
Full Name
Anti-CLTC Antibody, Rabbit Polyclonal
Gene Names
CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of EIF4A1 using anti-EIF4A1 antibody.)
EIF4A1, Polyclonal Antibody (Cat# AAA19205)
Full Name
Anti-EIF4A1 Antibody
Gene Names
EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-MFAP3 antibody (AAA19989).Overlay histogram showing 293T cells stained with AAA19989 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFAP3 Antibody (AAA19989, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MFAP3, Polyclonal Antibody (Cat# AAA19989)
Full Name
Anti-MFAP3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 6. Western blot analysis of ABCE1 using anti-ABCE1 antibody (AAA19808).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human U20S whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: mouse 4T1 whole cell lysates,Lane 5: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (AAA19808) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.)
ABCE1, Polyclonal Antibody (Cat# AAA19808)
Full Name
Anti-ABCE1 Antibody Picoband
Gene Names
ABCE1; RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
IRS-1, Polyclonal Antibody (Cat# AAA29051)
Full Name
IRS-1 Polyclonal Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
Napsin A, Polyclonal Antibody (Cat# AAA29375)
Full Name
Napsin A Polyclonal Antibody
Gene Names
NAPSA; KAP; Kdap; NAP1; NAPA; SNAPA
Reactivity
Human
Applications
IHC-P
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MAP1LC3A at 1:1000 dilution.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA30594)
Full Name
MAP1LC3A Rabbit Polyclonal Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using ATF5 antibody.)
ATF5, Polyclonal Antibody (Cat# AAA30665)
Full Name
ATF5 Rabbit Polyclonal Antibody
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using KRT5 Antibody at dilution of 1:100 (40x lens).)
KRT5, Polyclonal Antibody (Cat# AAA10706)
Full Name
KRT5 Polyclonal Antibody
Gene Names
KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using SFPQ antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
SFPQ, Polyclonal Antibody (Cat# AAA10716)
Full Name
SFPQ Polyclonal Antibody
Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using OPA1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
OPA1, Polyclonal Antibody (Cat# AAA30716)
Full Name
OPA1 Rabbit Polyclonal Antibody
Gene Names
OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HeLa cells using anti-Nucleostemin/GNL3 antibody (AAA19482).Overlay histogram showing HeLa cells stained with AAA19482 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nucleostemin/GNL3 Antibody (AAA19482, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Nucleostemin/GNL3, Polyclonal Antibody (Cat# AAA19482)
Full Name
Anti-Nucleostemin/GNL3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.