Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-IDUA antibody (AAA19772).Overlay histogram showing SH-SY5Y cells stained with AAA19772 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDUA Antibody (AAA19772, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of IDUA using anti-IDUA antibody (AAA19772).IDUA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDUA Antibody (AAA19772) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human U87 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDUA antigen affinity purified polyclonal antibody (#AAA19772) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDUA at approximately 73 kDa. The expected band size for IDUA is at 73 kDa.)

IDUA, Polyclonal Antibody (Cat# AAA19772)

Full Name

Anti-IDUA Antibody Picoband

Gene Names

IDUA; IDA; MPS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3 ug PIK3C2A Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PIK3C2A Polyclonal Antibody at a dilution of 1:1000.)

PIK3C2A, Polyclonal Antibody (Cat# AAA22280)

Full Name

PIK3C2A Polyclonal Antibody

Gene Names

PIK3C2A; CPK; PI3-K-C2A; PI3-K-C2(ALPHA)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (CRFR2 Antibody (D35) flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CRHR2 / CRF2 Receptor, Polyclonal Antibody (Cat# AAA21272)

Full Name

Anti-CRHR2 / CRF2 Receptor Antibody (aa10-39) IHC-plus

Gene Names

CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Protein A purified

Pricing

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WB (Western Blot) (WB Suggested Anti-ERLIN2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate.ERLIN2 is strongly supported by BioGPS gene expression data to be expressed in HEK293T)

ERLIN2, Polyclonal Antibody (Cat# AAA23581)

Full Name

ERLIN2 antibody - middle region

Gene Names

ERLIN2; NET32; SPFH2; SPG18; C8orf2; Erlin-2

Reactivity

Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CEBPG antibody.)

CEBPG, Polyclonal Antibody (Cat# AAA28103)

Full Name

CEBPG Polyclonal Antibody

Gene Names

CEBPG; GPE1BP; IG/EBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

TEAD1, Polyclonal Antibody (Cat# AAA28264)

Full Name

TEAD1 Polyclonal Antibody

Gene Names

TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

MMP9, Polyclonal Antibody (Cat# AAA28681)

Full Name

MMP9 Antibody (C-term)

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

VIM, Polyclonal Antibody (Cat# AAA29137)

Full Name

VIM Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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WB (Western Blot) (Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

PAK1, Polyclonal Antibody (Cat# AAA31096)

Full Name

PAK1 Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody at dilution of 1:100 .)

IGF2R, Polyclonal Antibody (Cat# AAA30559)

Full Name

IGF2R Polyclonal Antibody

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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ICC (Immunocytochemistry) (Anti- SLC2A1 Picoband antibody, AAA11642, ICCICC: A549 Cell)

SLC2A1, Polyclonal Antibody (Cat# AAA11642)

Full Name

Anti-SLC2A1 Antibody

Gene Names

SLC2A1; CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U2OS cells using anti-RBP1 antibody (AAA19800).Overlay histogram showing U2OS cells stained with AAA19800 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBP1 Antibody (AAA19800, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of RBP1 using anti-RBP1 antibody (AAA19800) and anti-Tubulin Alpha antibody (M03989-3).RBP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP1 antigen affinity purified polyclonal antibody (#AAA19800) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBP1 at approximately 16 kDa. The expected band size for RBP1 is at 16 kDa.)

RBP1, Polyclonal Antibody (Cat# AAA19800)

Full Name

Anti-RBP1 Antibody Picoband

Gene Names

RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HEL cells using anti-AHRR antibody (AAA19751).Overlay histogram showing HEL cells stained with AAA19751 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHRR Antibody (AAA19751, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of AHRR using anti-AHRR antibody (AAA19751) and anti-Beta Tubulin antibody (M01857-3).AHRR was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-AHRR Antibody (AAA19751) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human SiHa whole cell lysates,Lane 4: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHRR antigen affinity purified polyclonal antibody (#AAA19751) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AHRR at approximately 100 kDa. The expected band size for AHRR is at 76 kDa.)

AHRR, Polyclonal Antibody (Cat# AAA19751)

Full Name

Anti-AHRR Antibody Picoband

Gene Names

AHRR; AHH; AHHR; bHLHe77

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Host: RabbitTarget Name: GLI2Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 2.5ug/mLPeptide Concentration: 4.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 6%-18%)

GLI2, Polyclonal Antibody (Cat# AAA23396)

Full Name

GLI2 antibody - middle region

Gene Names

GLI2; CJS; HPE9; PHS2; THP1; THP2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

OPG, Polyclonal Antibody (Cat# AAA29085)

Full Name

OPG Polyclonal Antibody

Gene Names

TNFRSF11B; OPG; TR1; OCIF

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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GLUD1, Polyclonal Antibody (Cat# AAA30691)

Full Name

GLUD1 Rabbit Polyclonal Antibody

Gene Names

GLUD1; GDH; GDH1; GLUD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-thyroid, antibody was diluted at 1:200)

Thyroglobulin, Polyclonal Antibody (Cat# AAA30767)

Full Name

Thyroglobulin Rabbit Polyclonal Antibody

Gene Names

TG; TGN; AITD3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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MAP1LC3B, Polyclonal Antibody (Cat# AAA28176)

Full Name

MAP1LC3B Polyclonal Antibody

Gene Names

MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CHRFAM7A antibody at dilution of 1:100 (40x lens).)

CHRFAM7A, Polyclonal Antibody (Cat# AAA28208)

Full Name

CHRFAM7A Polyclonal Antibody

Gene Names

CHRFAM7A; D-10; CHRNA7; CHRNA7-DR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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SNAIL, Polyclonal Antibody (Cat# AAA31310)

Full Name

Phospho-SNAIL (Ser11) Antibody

Gene Names

SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26904)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

Purified by Immunoaffinity chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Bax Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.)

Bax, Polyclonal Antibody (Cat# AAA28318)

Full Name

Bax Rabbit pAb

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

LIR-7, Polyclonal Antibody (Cat# AAA29264)

Full Name

LIR-7 Polyclonal Antibody

Gene Names

LILRA2; ILT1; LIR7; CD85H; LIR-7

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

NF2, Polyclonal Antibody (Cat# AAA29080)

Full Name

NF2 Polyclonal Antibody

Gene Names

NF2; ACN; SCH; BANF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

NY-CO-1, Polyclonal Antibody (Cat# AAA29083)

Full Name

NY-CO-1 Polyclonal Antibody

Gene Names

NEMF; NY-CO-1; SDCCAG1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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WB (Western Blot) (Western blot of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 ug/ml.)

TLR3, Polyclonal Antibody (Cat# AAA12329)

Full Name

Rabbit Polyclonal to Human TLR3

Gene Names

TLR3; CD283; IIAE2

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Ion exchange Chromatography

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CHK2, Polyclonal Antibody (Cat# AAA31398)

Full Name

Phospho-CHK2 (Ser33+Ser35) Antibody

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (82%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

Pyruvate Dehydrogenase Complex Component X, Polyclonal Antibody (Cat# AAA20946)

Full Name

Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)

Gene Names

PDHX; E3BP; OPDX; PDX1; proX; DLDBP; PDHXD

Reactivity

Human, Pig

Applications

WB, ICC, IHC, EIA

Purity

Affinity Chromatography

Pricing

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IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA11682)

Full Name

Anti-Cytokeratin 18 Antibody

Gene Names

KRT18; K18; CK-18; CYK18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody.)

UBR2, Polyclonal Antibody (Cat# AAA19207)

Full Name

Anti-UBR2 Antibody

Gene Names

UBR2; C6orf133; bA49A4.1; dJ242G1.1; dJ392M17.3

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of NRK cells using anti-SHANK3 antibody (AAA19237).Overlay histogram showing NRK cells stained with AAA19237 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHANK3 Antibody (AAA19237,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SHANK3, Polyclonal Antibody (Cat# AAA19237)

Full Name

Anti-SHANK3 Antibody

Gene Names

SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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hnRNP U/p120/HNRNPU, Polyclonal Antibody (Cat# AAA19498)

Full Name

Anti-hnRNP U/p120/HNRNPU Antibody Picoband

Gene Names

HNRNPU; HNRPU; SAF-A; U21.1; hnRNP U

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Cortactin Polyclonal Antibody at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.)

Cortactin, Polyclonal Antibody (Cat# AAA22289)

Full Name

Cortactin Polyclonal Antibody

Gene Names

CTTN; EMS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug FUS antibody. Western blot was performed from the immunoprecipitate using FUS at a dilution of 1:1000.)

FUS, Polyclonal Antibody (Cat# AAA28431)

Full Name

FUS Rabbit pAb

Gene Names

MAFK; P18; NFE2U

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

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ICC (Immunocytochemistry) (ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Calpain 1, Polyclonal Antibody (Cat# AAA29883)

Full Name

Calpain 1 Antibody

Gene Names

CAPN1; CANP; muCL; CANP1; CANPL1; muCANP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

ProA affinity purified

Pricing

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AMH, Polyclonal Antibody (Cat# AAA29664)

Full Name

AMH Antibody

Gene Names

AMH; MIF; MIS

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen affinity purification.

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

EGF, Polyclonal Antibody (Cat# AAA29350)

Full Name

EGF Polyclonal Antibody

Gene Names

EGF; URG; HOMG4

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Tubulin alpha, Polyclonal Antibody (Cat# AAA29228)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

alpha 1 Catenin, Polyclonal Antibody (Cat# AAA31319)

Full Name

Phospho-alpha 1 Catenin (Ser655/Thr658) Antibody

Gene Names

CTNNA1; CAP102

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

HSP A5, Polyclonal Antibody (Cat# AAA29044)

Full Name

HSP A5 Polyclonal Antibody

Gene Names

HSPA5; BIP; MIF2; GRP78

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

p53, Polyclonal Antibody (Cat# AAA28842)

Full Name

p53 (Di Methyl Lys370) Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using IKBKG antibody. Blue: DAPI for nuclear staining.)

IKBKG, Polyclonal Antibody (Cat# AAA10636)

Full Name

IKBKG Polyclonal Antibody

Gene Names

IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MYO1C antibody. Blue: DAPI for nuclear staining.)

MYO1C, Polyclonal Antibody (Cat# AAA10776)

Full Name

MYO1C Polyclonal Antibody

Gene Names

MYO1C; NMI; MMIb; myr2; MMI-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

GPER1 / GPR30, Polyclonal Antibody (Cat# AAA12334)

Full Name

Rabbit Polyclonal to Human GPER1 / GPR30

Gene Names

GPER1; mER; CEPR; GPER; DRY12; FEG-1; GPR30; LERGU; LyGPR; CMKRL2; LERGU2; GPCR-Br

Reactivity

Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

RNF213/C17orf27, Polyclonal Antibody (Cat# AAA13646)

Full Name

Goat anti-RNF213/C17orf27 (C Terminus) Antibody

Gene Names

RNF213; ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Peptide ELISA, Immunohistochemistry

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

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WB (Western Blot) (Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)

H2AFM/HIST1H2AB, Polyclonal Antibody (Cat# AAA27772)

Full Name

Anti-H2AFM/HIST1H2AB Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Protein A & Antigen Affinity

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

Full Name

GAPDH Antibody (N-term)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using VDAC2 antibody.)

VDAC2, Polyclonal Antibody (Cat# AAA30728)

Full Name

VDAC2 Rabbit Polyclonal Antibody

Gene Names

VDAC2; POR

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using GABARAP antibody at dilution of 1:100 (40x lens).)

GABARAP, Polyclonal Antibody (Cat# AAA28252)

Full Name

GABARAP Polyclonal Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using ERLIN1 antibody.)

ERLIN1, Polyclonal Antibody (Cat# AAA29813)

Full Name

ERLIN1 Polyclonal Antibody

Gene Names

ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 900-950 of 10000 product results

IDUA, Polyclonal Antibody (Cat# AAA19772)

PIK3C2A, Polyclonal Antibody (Cat# AAA22280)

CRHR2 / CRF2 Receptor, Polyclonal Antibody (Cat# AAA21272)

ERLIN2, Polyclonal Antibody (Cat# AAA23581)

CEBPG, Polyclonal Antibody (Cat# AAA28103)

TEAD1, Polyclonal Antibody (Cat# AAA28264)

MMP9, Polyclonal Antibody (Cat# AAA28681)

VIM, Polyclonal Antibody (Cat# AAA29137)

PAK1, Polyclonal Antibody (Cat# AAA31096)

IGF2R, Polyclonal Antibody (Cat# AAA30559)

SLC2A1, Polyclonal Antibody (Cat# AAA11642)

RBP1, Polyclonal Antibody (Cat# AAA19800)

AHRR, Polyclonal Antibody (Cat# AAA19751)

GLI2, Polyclonal Antibody (Cat# AAA23396)

OPG, Polyclonal Antibody (Cat# AAA29085)

GLUD1, Polyclonal Antibody (Cat# AAA30691)

Thyroglobulin, Polyclonal Antibody (Cat# AAA30767)

MAP1LC3B, Polyclonal Antibody (Cat# AAA28176)

CHRFAM7A, Polyclonal Antibody (Cat# AAA28208)

SNAIL, Polyclonal Antibody (Cat# AAA31310)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26904)

Bax, Polyclonal Antibody (Cat# AAA28318)

LIR-7, Polyclonal Antibody (Cat# AAA29264)

NF2, Polyclonal Antibody (Cat# AAA29080)

NY-CO-1, Polyclonal Antibody (Cat# AAA29083)

TLR3, Polyclonal Antibody (Cat# AAA12329)

CHK2, Polyclonal Antibody (Cat# AAA31398)

Pyruvate Dehydrogenase Complex Component X, Polyclonal Antibody (Cat# AAA20946)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA11682)

UBR2, Polyclonal Antibody (Cat# AAA19207)

SHANK3, Polyclonal Antibody (Cat# AAA19237)

hnRNP U/p120/HNRNPU, Polyclonal Antibody (Cat# AAA19498)

Cortactin, Polyclonal Antibody (Cat# AAA22289)

FUS, Polyclonal Antibody (Cat# AAA28431)

Calpain 1, Polyclonal Antibody (Cat# AAA29883)

AMH, Polyclonal Antibody (Cat# AAA29664)

EGF, Polyclonal Antibody (Cat# AAA29350)

Tubulin alpha, Polyclonal Antibody (Cat# AAA29228)

alpha 1 Catenin, Polyclonal Antibody (Cat# AAA31319)

HSP A5, Polyclonal Antibody (Cat# AAA29044)

p53, Polyclonal Antibody (Cat# AAA28842)

IKBKG, Polyclonal Antibody (Cat# AAA10636)

MYO1C, Polyclonal Antibody (Cat# AAA10776)

GPER1 / GPR30, Polyclonal Antibody (Cat# AAA12334)

RNF213/C17orf27, Polyclonal Antibody (Cat# AAA13646)

H2AFM/HIST1H2AB, Polyclonal Antibody (Cat# AAA27772)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

VDAC2, Polyclonal Antibody (Cat# AAA30728)

GABARAP, Polyclonal Antibody (Cat# AAA28252)

ERLIN1, Polyclonal Antibody (Cat# AAA29813)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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