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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-MOCS2 antibody (AAA19885).Overlay histogram showing JK cells stained with AAA19885 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOCS2 Antibody (AAA19885, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MOCS2 using anti-MOCS2 antibody (AAA19885).MOCS2 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOCS2 Antibody (AAA19885) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOCS2 Antibody (AAA19885) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOCS2 Antibody (AAA19885) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOCS2 Antibody (AAA19885) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human LNCAP whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: human Jurkat whole cell lysates,Lane 6: human K562 whole cell lysates,Lane 7: human HepG2 whole cell lysates,Lane 8: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS2 antigen affinity purified polyclonal antibody (#AAA19885) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MOCS2 at approximately 21 kDa. The expected band size for MOCS2 is at 21 kDa.)

MOCS2, Polyclonal Antibody (Cat# AAA19885)

Full Name

Anti-MOCS2 Antibody Picoband

Gene Names

MOCS2; MPTS; MCBPE; MOCO1; MOCODB

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using HNRNPD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HNRNPD, Polyclonal Antibody (Cat# AAA22189)

Full Name

HNRNPD Polyclonal Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using APP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

APP, Polyclonal Antibody (Cat# AAA28326)

Full Name

APP Rabbit pAb

Gene Names

APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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CX3CR1, Polyclonal Antibody (Cat# AAA10919)

Full Name

CX3CR1 Antibody

Gene Names

CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

CX3CR1 Antibody is affinity chromatography purified via peptide column.

Pricing

H7 (H7N9)(A/Shanghai/2/2013), Polyclonal Antibody (Cat# AAA13735)

Full Name

Anti-H7 (H7N9)(A/Shanghai/2/2013)

Applications

Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Purity

Immunoaffinity chromatography

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-QARS1 antibody (AAA19879).Overlay histogram showing THP-1 cells stained with AAA19879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QARS1 Antibody (AAA19879, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of QARS1 using anti-QARS1 antibody (AAA19879).QARS1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat RH35 whole cell lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QARS1 antigen affinity purified polyclonal antibody (#AAA19879) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for QARS1 at approximately 88 kDa. The expected band size for QARS1 is at 88 kDa.)

QARS1, Polyclonal Antibody (Cat# AAA19879)

Full Name

Anti-QARS1 Antibody Picoband

Gene Names

QARS; GLNRS; MSCCA; PRO2195

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

PERK, Polyclonal Antibody (Cat# AAA29096)

Full Name

PERK Polyclonal Antibody

Gene Names

EIF2AK3; PEK; WRS; PERK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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FOXO4, Polyclonal Antibody (Cat# AAA31400)

Full Name

Phospho-FOXO4 (Thr455) Antibody

Gene Names

FOXO4; AFX; AFX1; MLLT7

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (91%), Horse (83%), Sheep (91%), Dog (91%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CCDC59 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CCDC59, Polyclonal Antibody (Cat# AAA22181)

Full Name

CCDC59 Polyclonal Antibody

Gene Names

CCDC59; BR22; TAP26; HSPC128

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-SOX2 antibody Titration: 1 ug/mLSample Type: Human OVCAR-3)

SOX2, Polyclonal Antibody (Cat# AAA23461)

Full Name

SOX2 antibody - middle region

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2OS cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Lipoprotein lipase (LPL), Polyclonal Antibody (Cat# AAA28325)

Full Name

Lipoprotein lipase (LPL) Rabbit pAb

Gene Names

LPL; LIPD; HDLCQ11

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

PBF, Polyclonal Antibody (Cat# AAA29093)

Full Name

PBF Polyclonal Antibody

Gene Names

PTTG1IP; PBF; C21orf1; C21orf3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

Endoglin, Polyclonal Antibody (Cat# AAA29349)

Full Name

Endoglin Polyclonal Antibody

Gene Names

ENG; END; ORW; HHT1; ORW1; CD105

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HNRNPD at 1:1000 dilution.)

HNRNPD, Polyclonal Antibody (Cat# AAA30631)

Full Name

HNRNPD Rabbit Polyclonal Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Green: RSPO1 antibody (5171)Red: Phylloidin stainingBlue: DAPI staining)

RSPO1, Polyclonal Antibody (Cat# AAA10920)

Full Name

RSPO1 Antibody

Gene Names

RSPO1; RSPO; CRISTIN3

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

RSPO1 Antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ZNF177 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ZNF177, Polyclonal Antibody (Cat# AAA22184)

Full Name

ZNF177 Polyclonal Antibody

Gene Names

ZNF177; PIGX

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PFDN4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

PFDN4, Polyclonal Antibody (Cat# AAA22187)

Full Name

PFDN4 Polyclonal Antibody

Gene Names

PFDN4; C1; PFD4

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications.)

Histone H3, Polyclonal Antibody (Cat# AAA28843)

Full Name

Histone H3 (Di Methyl Lys10) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

PKC, Polyclonal Antibody (Cat# AAA29099)

Full Name

PKC Polyclonal Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IP (Immunoprecipitation) (Figure 10. Immunoprecipitating TNS3 in U251 whole cell lysate .Western blot analysis of TNS3 using anti-TNS3 antibody (AAA19884).Lane 1: U251 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-TNS3 antibody in U251 whole cell lysate,Lane 3: anti-TNS3 antibody (2ug) + U251 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (AAA19884) at a dilution of 0.5ug/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.)

IF (Immunofluorescence) (Figure 8. IF analysis of TNS3 using anti-TNS3 antibody (AAA19884).TNS3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TNS3 Antibody (AAA19884) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (#AAA19884) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.)

TNS3, Polyclonal Antibody (Cat# AAA19884)

Full Name

Anti-TNS3 Antibody Picoband

Gene Names

TNS3; TEM6; TENS1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.)

TFF3, Polyclonal Antibody (Cat# AAA10911)

Full Name

TFF3 Antibody

Gene Names

TFF3; ITF; P1B; TFI

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

TFF3 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse heart using DES Polyclonal Antibody at dilution of 1:100 (40x lens).)

DES, Polyclonal Antibody (Cat# AAA22175)

Full Name

DES Polyclonal Antibody

Gene Names

DES; CSM1; CSM2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ZNF703 at dilution of 1:100 (40x lens).)

ZNF703, Polyclonal Antibody (Cat# AAA30623)

Full Name

ZNF703 Rabbit Polyclonal Antibody

Gene Names

ZNF703; NLZ1; ZPO1; ZEPPO1; ZNF503L

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

DNMT1, Polyclonal Antibody (Cat# AAA22199)

Full Name

DNMT1 Polyclonal Antibody

Gene Names

DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-THOC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

THOC1, Polyclonal Antibody (Cat# AAA23479)

Full Name

THOC1 Antibody - C-terminal region

Gene Names

THOC1; P84; HPR1; P84N5

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Ribosomal Protein L7, Polyclonal Antibody (Cat# AAA29111)

Full Name

Ribosomal Protein L7 Polyclonal Antibody

Gene Names

RPL7; L7; humL7-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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ICC (Immunocytochemistry) (ICC staining HSC70 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Hsc70, Polyclonal Antibody (Cat# AAA29879)

Full Name

Hsc70 Antibody

Gene Names

HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Peptide affinity purified

Pricing

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IHC (Immunohistchemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BRCA1, Polyclonal Antibody (Cat# AAA31415)

Full Name

Phospho-BRCA1 (Ser1497) Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (80%), Dog (80%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of THP-1 cells using anti-LIN7C antibody (AAA19897).Overlay histogram showing THP-1 cells stained with AAA19897 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LIN7C Antibody (AAA19897, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of LIN7C using anti-LIN7C antibody (AAA19897).LIN7C was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LIN7C Antibody (AAA19897) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LIN7C Antibody (AAA19897) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LIN7C Antibody (AAA19897) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LIN7C Antibody (AAA19897) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody (#AAA19897) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LIN7C at approximately 22 kDa. The expected band size for LIN7C is at 22 kDa.)

LIN7C, Polyclonal Antibody (Cat# AAA19897)

Full Name

Anti-LIN7C Antibody Picoband

Gene Names

LIN7C; MALS3; VELI3; LIN-7C; MALS-3; LIN-7-C

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using CD44 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CD44, Polyclonal Antibody (Cat# AAA22201)

Full Name

CD44 Polyclonal Antibody

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using USP18 antibody.)

USP18, Polyclonal Antibody (Cat# AAA30649)

Full Name

USP18 Rabbit Polyclonal Antibody

Gene Names

USP18; ISG43; UBP43

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of Phospho-MOB1A (Tyr26) in lysates of 293 IFN 2500U/ml 30', using Phospho-MOB1A (Tyr26) Antibody.)

MOB1A, Polyclonal Antibody (Cat# AAA31161)

Full Name

Phospho-MOB1A (Tyr26) Antibody

Gene Names

MOB1B; MATS2; MOB4A; MOBKL1A

Reactivity

Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of TLR3 expression in mouse spleen tissue lysate with TLR3 antibody at (A) 0.5 and (B) 1 μg/ml.)

TLR3, Polyclonal Antibody (Cat# AAA10938)

Full Name

TLR3 Antibody

Gene Names

TLR3; CD283; IIAE2

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

TLR3 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

AR, Polyclonal Antibody (Cat# AAA31420)

Full Name

Phospho-AR (Tyr535) Antibody

Gene Names

AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (WB Suggested Anti-PUF60 Antibody Titration: 0.2-1 ug/mlPositive Control: Hela cell lysatePUF60 is supported by BioGPS gene expression data to be expressed in HeLa)

PUF60, Polyclonal Antibody (Cat# AAA23485)

Full Name

PUF60 antibody - C-terminal region

Gene Names

PUF60; FIR; VRJS; RoBPI; SIAHBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

EPX, Polyclonal Antibody (Cat# AAA23997)

Full Name

EPX antibody

Gene Names

EPX; EPO; EPP; EPX-PEN

Purity

Affinity purified

Pricing

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FBXL2, Polyclonal Antibody (Cat# AAA29885)

Full Name

FBXL2 Antibody

Gene Names

FBXL2; FBL2; FBL3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Figure 11. IF analysis of SPA17 using anti-SPA17 antibody (AAA19898).SPA17 was detected in a paraffin-embedded section of human ovarian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SPA17 using anti-SPA17 antibody (AAA19898).SPA17 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SPA17 Antibody (AAA19898) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPA17 antigen affinity purified polyclonal antibody (#AAA19898) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SPA17 at approximately 22 kDa. The expected band size for SPA17 is at 17 kDa.)

SPA17, Polyclonal Antibody (Cat# AAA19898)

Full Name

Anti-SPA17 Antibody Picoband

Gene Names

SPA17; CT22; SP17; SP17-1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ENO1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ENO1, Polyclonal Antibody (Cat# AAA22202)

Full Name

ENO1 Polyclonal Antibody

Gene Names

ENO1; NNE; PPH; MPB1; ENO1L1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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MSH6, Polyclonal Antibody (Cat# AAA10686)

Full Name

MSH6 Polyclonal Antibody

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Mouse, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence of PD-L2 in mouse brain tissue with PD-L2 antibody at 20 μg/ml.Green: PD-L2 Antibody (4063)Blue: DAPI staining)

PDL-2, Polyclonal Antibody (Cat# AAA10942)

Full Name

PDL-2 Antibody

Gene Names

PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

PDL-2 Antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SMAD2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

SMAD2, Polyclonal Antibody (Cat# AAA22206)

Full Name

SMAD2 Polyclonal Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

PAXIP1, Polyclonal Antibody (Cat# AAA28350)

Full Name

PAXIP1 Rabbit pAb

Gene Names

PAXIP1; PTIP; TNRC2; CAGF28; CAGF29; PACIP1; PAXIP1L

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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COL1A1, Polyclonal Antibody (Cat# AAA30654)

Full Name

COL1A1 Rabbit Polyclonal Antibody

Gene Names

COL1A1; OI4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BCLAF1, Polyclonal Antibody (Cat# AAA31422)

Full Name

Phospho-BCLAF1 (Ser531) Antibody

Gene Names

BCLAF1; BTF; bK211L9.1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SHMT2 antibody. Blue: DAPI for nuclear staining.)

SHMT2, Polyclonal Antibody (Cat# AAA10687)

Full Name

SHMT2 Polyclonal Antibody

Gene Names

SHMT2; GLYA; SHMT; HEL-S-51e

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 using TOM20 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

TOM20, Polyclonal Antibody (Cat# AAA22204)

Full Name

TOM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)

Ran, Polyclonal Antibody (Cat# AAA19131)

Full Name

Anti-Ran Picoband Antibody

Gene Names

RAN; TC4; Gsp1; ARA24

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HL-60 cells using anti-RNF34 antibody (A07903-1).Overlay histogram showing HL-60 cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF34 Antibody (A07903-1, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RNF34 and Tubulin beta using anti-RNF34 antibody (A07903-1) and anti-Tubulin beta antibody (M05613-4).RNF34 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (#AAA19899) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RNF34 at approximately 42 kDa. The expected band size for RNF34 is at 42 kDa.)

RNF34, Polyclonal Antibody (Cat# AAA19899)

Full Name

Anti-RNF34 Antibody Picoband

Gene Names

RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)

PNKP, Polyclonal Antibody (Cat# AAA28091)

Full Name

PNKP Polyclonal Antibody

Gene Names

PNKP; PNK; MCSZ; EIEE10

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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MOCS2, Polyclonal Antibody (Cat# AAA19885)

HNRNPD, Polyclonal Antibody (Cat# AAA22189)

APP, Polyclonal Antibody (Cat# AAA28326)

CX3CR1, Polyclonal Antibody (Cat# AAA10919)

H7 (H7N9)(A/Shanghai/2/2013), Polyclonal Antibody (Cat# AAA13735)

QARS1, Polyclonal Antibody (Cat# AAA19879)

PERK, Polyclonal Antibody (Cat# AAA29096)

FOXO4, Polyclonal Antibody (Cat# AAA31400)

CCDC59, Polyclonal Antibody (Cat# AAA22181)

SOX2, Polyclonal Antibody (Cat# AAA23461)

Lipoprotein lipase (LPL), Polyclonal Antibody (Cat# AAA28325)

PBF, Polyclonal Antibody (Cat# AAA29093)

Endoglin, Polyclonal Antibody (Cat# AAA29349)

HNRNPD, Polyclonal Antibody (Cat# AAA30631)

RSPO1, Polyclonal Antibody (Cat# AAA10920)

ZNF177, Polyclonal Antibody (Cat# AAA22184)

PFDN4, Polyclonal Antibody (Cat# AAA22187)

Histone H3, Polyclonal Antibody (Cat# AAA28843)

PKC, Polyclonal Antibody (Cat# AAA29099)

TNS3, Polyclonal Antibody (Cat# AAA19884)

TFF3, Polyclonal Antibody (Cat# AAA10911)

DES, Polyclonal Antibody (Cat# AAA22175)

ZNF703, Polyclonal Antibody (Cat# AAA30623)

DNMT1, Polyclonal Antibody (Cat# AAA22199)

THOC1, Polyclonal Antibody (Cat# AAA23479)

Ribosomal Protein L7, Polyclonal Antibody (Cat# AAA29111)

Hsc70, Polyclonal Antibody (Cat# AAA29879)

BRCA1, Polyclonal Antibody (Cat# AAA31415)

LIN7C, Polyclonal Antibody (Cat# AAA19897)

CD44, Polyclonal Antibody (Cat# AAA22201)

USP18, Polyclonal Antibody (Cat# AAA30649)

MOB1A, Polyclonal Antibody (Cat# AAA31161)

TLR3, Polyclonal Antibody (Cat# AAA10938)

AR, Polyclonal Antibody (Cat# AAA31420)

PUF60, Polyclonal Antibody (Cat# AAA23485)

EPX, Polyclonal Antibody (Cat# AAA23997)

FBXL2, Polyclonal Antibody (Cat# AAA29885)

SPA17, Polyclonal Antibody (Cat# AAA19898)

ENO1, Polyclonal Antibody (Cat# AAA22202)

MSH6, Polyclonal Antibody (Cat# AAA10686)

PDL-2, Polyclonal Antibody (Cat# AAA10942)

SMAD2, Polyclonal Antibody (Cat# AAA22206)

PAXIP1, Polyclonal Antibody (Cat# AAA28350)

COL1A1, Polyclonal Antibody (Cat# AAA30654)

BCLAF1, Polyclonal Antibody (Cat# AAA31422)

SHMT2, Polyclonal Antibody (Cat# AAA10687)

TOM20, Polyclonal Antibody (Cat# AAA22204)

Ran, Polyclonal Antibody (Cat# AAA19131)

RNF34, Polyclonal Antibody (Cat# AAA19899)

PNKP, Polyclonal Antibody (Cat# AAA28091)

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