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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 600-650 of 10000 product results
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
LRRK2, Polyclonal Antibody (Cat# AAA31287)
Full Name
Phospho-LRRK2 (Ser935) Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
MCM2, Polyclonal Antibody (Cat# AAA31460)
Full Name
Phospho-MCM2 (Ser27) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of OCLN in mouse brain tissue with OCLN Antibody at 5 μg/mL.)
OCLN, Polyclonal Antibody (Cat# AAA10929)
Full Name
OCLN Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
OCLN Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
((0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Orexin receptor 1, Polyclonal Antibody (Cat# AAA13669)
Full Name
Goat anti-Orexin receptor 1 Antibody
Gene Names
HCRTR1; OX1R
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(WB Suggested Anti-LYN Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysateLYN is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)
LYN, Polyclonal Antibody (Cat# AAA23560)
Full Name
LYN antibody - N-terminal region
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
hnRNP A2/B1, Polyclonal Antibody (Cat# AAA29040)
Full Name
hnRNP A2/B1 Polyclonal Antibody
Gene Names
HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; HNRPA2B1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ESRRA antibody at 1:1000 dilution.)
ESRRA, Polyclonal Antibody (Cat# AAA30567)
Full Name
ESRRA Polyclonal Antibody
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
p53, Polyclonal Antibody (Cat# AAA29278)
Full Name
p53 Polyclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31355)
Full Name
Acetyl-p53 (Lys381) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit (90%), Dog (100%)
Predicted Reactivity: Rabbit (90%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(Immunofluorescence-Akt2 Polyclonal Antibody)
Akt2, Polyclonal Antibody (Cat# AAA28287)
Full Name
Akt2 Polyclonal Antibody
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)
Cleaved-Caspase-3 p17, Polyclonal Antibody (Cat# AAA28830)
Full Name
Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with SIRT1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
ERK2, Polyclonal Antibody (Cat# AAA29921)
Full Name
ERK2 Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Ku-70, Polyclonal Antibody (Cat# AAA29224)
Full Name
Ku-70 Polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody at dilution of 1:100 .)
YAP1, Polyclonal Antibody (Cat# AAA30542)
Full Name
YAP1 Polyclonal Antibody
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using GGA2 antibody. Blue: DAPI for nuclear staining.)
GGA2, Polyclonal Antibody (Cat# AAA10705)
Full Name
GGA2 Polyclonal Antibody
Gene Names
GGA2; VEAR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
XRCC4, Polyclonal Antibody (Cat# AAA10755)
Full Name
XRCC4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)
KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody (Cat# AAA12370)
Full Name
Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Ammonium sulfate precipitation
Pricing
IHC (Immunohistchemistry)
(Anti-ESRRG / ERR Gamma antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
ESRRG / ERR Gamma, Polyclonal Antibody (Cat# AAA12326)
Full Name
Rabbit Polyclonal to Human ESRRG / ERR Gamma
Gene Names
ESRRG; ERR3; NR3B3; ERRgamma
Reactivity
Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Predicted Reactivity: Bat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Predicted Reactivity: Bat, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunochemical staining of alpha smooth muscle actin in mouse skeletal muscle with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.)
ACTA2, Polyclonal Antibody (Cat# AAA27714)
Full Name
Anti-ACTA2 Antibody, Rabbit Polyclonal
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilution Lane A: TRIM28 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:89 kDa Observed band size:115 kDa (We are unsure as to the identity of these extra bands.))
KAP1/TRIM28, Polyclonal Antibody (Cat# AAA27715)
Full Name
Anti-KAP1/TRIM28 Antibody, Rabbit Polyclonal
Gene Names
TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using beta Catenin antibody.)
beta Catenin, Polyclonal Antibody (Cat# AAA29797)
Full Name
beta Catenin Polyclonal Antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
NADPH Oxidase 4, Polyclonal Antibody (Cat# AAA29878)
Full Name
NADPH Oxidase 4 Antibody
Gene Names
NOX4; KOX; KOX-1; RENOX
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CD22, Polyclonal Antibody (Cat# AAA31418)
Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Predicted Reactivity: Horse (83%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p53, Polyclonal Antibody (Cat# AAA31429)
Full Name
Phospho-p53 (Ser392) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse
Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (88%)
Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (88%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
SIRT1, Polyclonal Antibody (Cat# AAA29115)
Full Name
SIRT1 Polyclonal Antibody
Gene Names
SIRT1; SIR2L1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in:Mouse brain tissue,Mouse skeletal muscle tissue,ZebrafishAll lanes: Bmp4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)
Bone morphogenetic protein 4, Polyclonal Antibody (Cat# AAA26924)
Full Name
Rabbit anti-human Bone morphogenetic protein 4 polyclonal Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
E-cadherin, Polyclonal Antibody (Cat# AAA29017)
Full Name
E-cadherin Polyclonal Antibody
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
MCP-3, Polyclonal Antibody (Cat# AAA29383)
Full Name
MCP-3 Polyclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
HPA1, Polyclonal Antibody (Cat# AAA29231)
Full Name
HPA1 Polyclonal Antibody
Gene Names
HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
TRAIL, Polyclonal Antibody (Cat# AAA29280)
Full Name
TRAIL Polyclonal Antibody
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
ICC (Immunocytochemistry)
(ICC staining HSC70 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Hsc70, Polyclonal Antibody (Cat# AAA29879)
Full Name
Hsc70 Antibody
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Peptide affinity purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Lamin A/C, Polyclonal Antibody (Cat# AAA31378)
Full Name
Lamin A/C Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA, Flow Cytometry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Flow cytometric analysis of SK-BR-3 cells using MICA Antibody (Center) (green) compared to an isotype control of rabbit IgG(blue). AAA28710 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
MICA, Polyclonal Antibody (Cat# AAA28710)
Full Name
MICA Antibody (Center)
Gene Names
MICA; MIC-A; PERB11.1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
TPO, Polyclonal Antibody (Cat# AAA29249)
Full Name
TPO Polyclonal Antibody
Gene Names
THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-RAE1 antibody (AAA19509).Overlay histogram showing HL-60 cells stained with AAA19509 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAE1 Antibody (AAA19509, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RAE1, Polyclonal Antibody (Cat# AAA19509)
Full Name
Anti-RAE1 Antibody Picoband
Gene Names
RAE1; MIG14; MRNP41; Mnrp41; dJ481F12.3; dJ800J21.1
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of Hela cells using anti-SSSCA1/ZNRD2 antibody (AAA19995).Overlay histogram showing Hela cells stained with AAA19995 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSSCA1/ZNRD2 Antibody (AAA19995, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SSSCA1/ZNRD2, Polyclonal Antibody (Cat# AAA19995)
Full Name
Anti-SSSCA1/ZNRD2 Antibody Picoband
Gene Names
SSSCA1; p27
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using THBS1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
THBS1, Polyclonal Antibody (Cat# AAA22313)
Full Name
THBS1 Polyclonal Antibody
Gene Names
THBS1; TSP; THBS; TSP1; TSP-1; THBS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of HeLa, HepG2 cells using Acetyl-NF?B-p65 (K314/K315) Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
NFkappaB-p65, Polyclonal Antibody (Cat# AAA30779)
Full Name
NFkappaB-p65 (Acetyl-Lys314/Lys315) Rabbit Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
p38 MAPK, Polyclonal Antibody (Cat# AAA31333)
Full Name
Phospho-p38 MAPK (Thr180/Tyr182) Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 20 μg/ml.Green: Caspase-12 Antibody (Large) (3195)Blue: DAPI staining)
Caspase-12, Polyclonal Antibody (Cat# AAA10958)
Full Name
Caspase-12 Antibody (Large)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
Pricing
Transfection Control
(Anti-GPR45 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)
GPR45, Polyclonal Antibody (Cat# AAA12368)
Full Name
Rabbit Polyclonal to Human GPR45
Gene Names
GPR45; PSP24; PSP24A; PSP24(ALPHA)
Reactivity
Gibbon, Gorilla, Horse, Human, Monkey
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD85e/h, Polyclonal Antibody (Cat# AAA29199)
Full Name
CD85e/h Polyclonal Antibody
Gene Names
LILRA2; ILT1; LIR7; CD85H; LIR-7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
FGFR1/2/3/4, Polyclonal Antibody (Cat# AAA31417)
Full Name
Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
PI 3-kinase p85/p55, Polyclonal Antibody (Cat# AAA28939)
Full Name
PI 3-kinase p85/p55 (phospho Tyr467/199) Polyclonal Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; p85-ALPHA
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
MEK-1/2, Polyclonal Antibody (Cat# AAA29065)
Full Name
MEK-1/2 Polyclonal Antibody
Gene Names
MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
MMP-13, Polyclonal Antibody (Cat# AAA29070)
Full Name
MMP-13 Polyclonal Antibody
Gene Names
MMP13; CLG3; MANDP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence of IL-17 in mouse thymus tissue with IL-17 Antibody at 20 μg/mL.)
IL-17, Polyclonal Antibody (Cat# AAA10926)
Full Name
IL-17 Antibody
Gene Names
IL17A; IL17; CTLA8; IL-17; IL-17A
Reactivity
Human, Mouse
Applications
Western Blot
Purity
IL-17 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using KRT20 Antibody at dilution of 1:100 (40x lens).)
KRT20, Polyclonal Antibody (Cat# AAA10645)
Full Name
KRT20 Polyclonal Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.)
HLA-B, Polyclonal Antibody (Cat# AAA28743)
Full Name
HLA-B Antibody (N-term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using TDP-43/TARDB Rabbit pAb.)
TARDBP, Polyclonal Antibody (Cat# AAA30615)
Full Name
TARDBP Rabbit Polyclonal Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.