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▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 300-350 of 10000 product results
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U87 cells using anti-Nesprin 2/SYNE2 antibody (AAA19782).Overlay histogram showing U87 cells stained with AAA19782 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin 2/SYNE2 Antibody (AAA19782, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Nesprin 2/SYNE2, Polyclonal Antibody (Cat# AAA19782)
Full Name
Anti-Nesprin 2/SYNE2 Antibody Picoband
Gene Names
SYNE2; NUA; EDMD5; Nesp2; TROPH; NUANCE; SYNE-2; Nesprin-2
Reactivity
Human, Monkey
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-IDH1 Antibody Titration: 1 ug/mlPositive Control: Fetal liver cell lysate)
IDH1, Polyclonal Antibody (Cat# AAA23561)
Full Name
IDH1 antibody - C-terminal region
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
POLR2A, Polyclonal Antibody (Cat# AAA31440)
Full Name
Phospho-POLR2A (Ser1616/Ser2) Antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26876)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (HRP)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of NogoA in mouse brain tissue with NogoA Antibody at 20 μg/mL.Green: NogoA antibody (4089)Red: Phylloidin stainingBlue: DAPI staining)
NogoA, Polyclonal Antibody (Cat# AAA10955)
Full Name
NogoA Antibody
Gene Names
RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NogoA Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of CTRP3 in Mouse Heart tissue with CTRP3 antibody at 10 μg/mL.)
CTRP3, Polyclonal Antibody (Cat# AAA10961)
Full Name
CTRP3 Antibody
Gene Names
C1QTNF3; CORS; CORCS; CTRP3; CORS26; C1ATNF3; CORS-26
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
CTRP3 Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)
Acetyl-Histone H2B-K5, Polyclonal Antibody (Cat# AAA28317)
Full Name
Acetyl-Histone H2B-K5 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human esophageal tissue using Rabbit anti HLA A antibody at a dilution of 1/100)
HLA A, Polyclonal Antibody (Cat# AAA12255)
Full Name
Rabbit anti HLA A
Gene Names
HLA-A; HLAA
Reactivity
Human, Mouse, Rat (Expected from Sequence)
Applications
Immunohistochemistry, Western Blot
Purity
Purified whole serum by affinity chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-CD44 antibody (AAA19722).Overlay histogram showing MCF-7 cells stained with AAA19722 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (AAA19722, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD44, Polyclonal Antibody (Cat# AAA19722)
Full Name
Anti-CD44 Antibody Picoband
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using DNMT3A Antibody at dilution of 1:100 (40x lens).)
DNMT3A, Polyclonal Antibody (Cat# AAA10707)
Full Name
DNMT3A Polyclonal Antibody
Gene Names
DNMT3A; TBRS; DNMT3A2; M.HsaIIIA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(C: 293 cell extract control; E: 293 cell expressing gp120 (Clade B))
gp120 (Clade B), Polyclonal Antibody (Cat# AAA13711)
Full Name
Anti-gp120 (Clade B)
Applications
Western Blot
Purity
Immunoaffinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
LT-beta, Polyclonal Antibody (Cat# AAA29369)
Full Name
LT-beta Polyclonal Antibody
Gene Names
LTB; p33; TNFC; TNFSF3
Reactivity
Human
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
Thyroglobulin, Polyclonal Antibody (Cat# AAA29373)
Full Name
Thyroglobulin Polyclonal Antibody
Gene Names
TG; TGN; AITD3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
HSP 90, Polyclonal Antibody (Cat# AAA28846)
Full Name
HSP 90 (Acetyl Lys292/284) Polyclonal Antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Figure 12. IF analysis of CPSF6 using anti-CPSF6 antibody (AAA19520).CPSF6 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CPSF6 Antibody (AAA19520) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CPSF6, Polyclonal Antibody (Cat# AAA19520)
Full Name
Anti-CPSF6 Antibody Picoband
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of K562 cells using anti-PSIP1 antibody (AAA19764).Overlay histogram showing K562 cells stained with AAA19764 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSIP1 Antibody (AAA19764, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PSIP1, Polyclonal Antibody (Cat# AAA19764)
Full Name
Anti-PSIP1 Antibody Picoband
Gene Names
PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using CK-18 Polyclonal Antibody at dilution of 1:100)
CK-18, Polyclonal Antibody (Cat# AAA22341)
Full Name
CK-18 Polyclonal Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence-Lamin B1 Polyclonal Antibody)
Lamin B1, Polyclonal Antibody (Cat# AAA28298)
Full Name
Lamin B1 Polyclonal Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Ki67, Polyclonal Antibody (Cat# AAA28343)
Full Name
Ki67 Rabbit pAb
Gene Names
MKI67; KIA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(Western Blot Data: C: 293 cell extract controlE: 293 cell expressing NS1, band at *)
NS1 (A/Vietnam/1203/2004)(H5N1), Polyclonal Antibody (Cat# AAA13723)
Full Name
Anti-NS1 (A/Vietnam/1203/2004)(H5N1)
Applications
Western Blot
Purity
Immunoaffinity chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Tuberin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.)
TSC2/Tuberin, Polyclonal Antibody (Cat# AAA29867)
Full Name
TSC2/Tuberin Antibody
Gene Names
TSC2; LAM; TSC4
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-eIF4A3 Polyclonal Antibody, Unconjugated(bs-14548R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
eIF4A3, Polyclonal Antibody (Cat# AAA28816)
Full Name
eIF4A3 Polyclonal Antibody
Gene Names
EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
Reactivity
Rat, Dog, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver)
GCLC, Polyclonal Antibody (Cat# AAA23555)
Full Name
GCLC antibody - N-terminal region
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
FAS, Polyclonal Antibody (Cat# AAA29022)
Full Name
FAS Polyclonal Antibody
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MSH6 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
MSH6, Polyclonal Antibody (Cat# AAA28426)
Full Name
MSH6 Rabbit pAb
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat, MK
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Lipoprotein lipase (LPL), Polyclonal Antibody (Cat# AAA28325)
Full Name
Lipoprotein lipase (LPL) Rabbit pAb
Gene Names
LPL; LIPD; HDLCQ11
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
HSF1, Polyclonal Antibody (Cat# AAA31391)
Full Name
Phospho-HSF1 (Ser307) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Connexin 43, Polyclonal Antibody (Cat# AAA29000)
Full Name
Connexin 43 Polyclonal Antibody
Gene Names
GJA1; HSS; CX43; GJAL; ODDD; AVSD3; HLHS1; DFNB38
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using CTSE antibody.)
CTSE, Polyclonal Antibody (Cat# AAA10712)
Full Name
CTSE Polyclonal Antibody
Gene Names
CTSE; CATE
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of AF7020 expression in various lysates)
HMGB1, Polyclonal Antibody (Cat# AAA31148)
Full Name
HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Rabbit, Dog
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using TXNRD1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
TXNRD1, Polyclonal Antibody (Cat# AAA30643)
Full Name
TXNRD1 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Cyclin B1, Polyclonal Antibody (Cat# AAA22301)
Full Name
Cyclin B1 Polyclonal Antibody
Gene Names
CCNB1; CCNB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(EREG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EREG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
EREG, Polyclonal Antibody (Cat# AAA28771)
Full Name
EREG Antibody (C-term)
Gene Names
EREG; ER
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Smad2, Polyclonal Antibody (Cat# AAA31433)
Full Name
Phospho-Smad2 (Ser465+Ser467) Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation)
(IP/WB analysis of mouse brain, rat brain membrane lysate and mouse brain membrane lysate, were resolved by electrophoresis, transferred to PVDF membrane and probed using AAA14705 (1 :500). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein AMYLOID OLIGOMER (-80kD).)
b-Amyloid, Oligomer A11, Polyclonal Antibody (Cat# AAA14705)
Full Name
b-Amyloid, Oligomer A11
Reactivity
Human, Mouse, Rat and Eukaryote
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
Purity
Serum
Pricing
IHC (Immunohistchemistry)
(CTNNB1/Beta Catenin Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))
CTNNB1/Beta Catenin, Polyclonal Antibody (Cat# AAA21342)
Full Name
CTNNB1/Beta Catenin Rabbit anti-Human Polyclonal Antibody
Gene Names
CTNNB1; CTNNB; MRD19; armadillo
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CD274 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
CD274, Polyclonal Antibody (Cat# AAA29874)
Full Name
CD274 (PD1L1) Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
WB (Western Blot)
Cytokeratin 20/Krt20, Polyclonal Antibody (Cat# AAA19202)
Full Name
Anti-Cytokeratin 20/Krt20 Antibody
Gene Names
Krt20; K20; Ck20; Ck-20; Krt21; 9030623C06Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of HepG2 cells using anti-MATR3 antibody (AAA19784).Overlay histogram showing HepG2 cells stained with AAA19784 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MATR3 Antibody (AAA19784, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MATR3, Polyclonal Antibody (Cat# AAA19784)
Full Name
Anti-MATR3 Antibody Picoband
Gene Names
MATR3; MPD2; ALS21; VCPDM
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Figure 6. Immunoprecipitating Thrombomodulin/THBD in A431 whole cell lysate .Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (AAA19747).Lane 1: A431 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-Thrombomodulin/THBD antibody in A431 whole cell lysate.Lane 3: anti-Thrombomodulin/THBD antibody (2ug) + A431 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (AAA19747) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Thrombomodulin/THBD Antibody (AAA19747, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Thrombomodulin/THBD, Polyclonal Antibody (Cat# AAA19747)
Full Name
Anti-Thrombomodulin/THBD Antibody Picoband
Gene Names
THBD; TM; THRM; AHUS6; BDCA3; CD141; THPH12
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-NFAT3 (Ser289) in lysates of HepG2 Ca2+ 40uM 30', using Phospho-NFAT3 (Ser289) Antibody.)
NFAT3, Polyclonal Antibody (Cat# AAA31157)
Full Name
Phospho-NFAT3 (Ser289) Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Reg IV, Polyclonal Antibody (Cat# AAA29108)
Full Name
Reg IV Polyclonal Antibody
Gene Names
REG4; GISP; RELP; REG-IV
Reactivity
Human
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)
TIMP-1, Polyclonal Antibody (Cat# AAA29132)
Full Name
TIMP-1 Polyclonal Antibody
Gene Names
TIMP1; EPA; EPO; HCI; CLGI; TIMP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.)
NLRP3, Polyclonal Antibody (Cat# AAA30558)
Full Name
NLRP3 Polyclonal Antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using AIFM1 antibody. Blue: DAPI for nuclear staining.)
AIFM1, Polyclonal Antibody (Cat# AAA10690)
Full Name
AIFM1 Polyclonal Antibody
Gene Names
AIFM1; AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
Application Data
(BALB/c mouse splenocytes were stained with Goat F(ab)2 Anti-Mouse Kappa-BIOT and Rat Anti-Mouse CD19-PE followed by Streptavidin-FITC.)
Goat F(ab')2 Anti-Mouse kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA14899)
Full Name
Goat F(ab')2 Anti-Mouse Kappa-LE/AF
Reactivity
Mouse
Applications
Flow Cytometry
Purity
Gel filtration chromatography of pepsin digested antibody
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using THOC1 at dilution of 1:100 (40x lens).)
THOC1, Polyclonal Antibody (Cat# AAA30702)
Full Name
THOC1 Rabbit Polyclonal Antibody
Gene Names
THOC1; P84; HPR1; P84N5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Anti-Adiponectin antibody, AAA11599, IHC(P)IHC(P): Human Placenta Tissue)
Adiponectin, Polyclonal Antibody (Cat# AAA11599)
Full Name
Anti-Adiponectin antibody
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of THP-1 cells using anti-ASC/TMS1/PYCARD antibody (AAA19733).Overlay histogram showing THP-1 cells stained with AAA19733 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASC/TMS1/PYCARD Antibody (AAA19733, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ASC/TMS1/PYCARD, Polyclonal Antibody (Cat# AAA19733)
Full Name
Anti-ASC/TMS1/PYCARD Antibody Picoband
Gene Names
PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-JCHAIN antibody (AAA19779).Overlay histogram showing A431 cells stained with AAA19779 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-JCHAIN Antibody (AAA19779, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
JCHAIN, Polyclonal Antibody (Cat# AAA19779)
Full Name
Anti-JCHAIN Antibody Picoband
Gene Names
IGJ; JCH; IGCJ
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.