Polyclonal Antibodies

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of HepG2 cells using anti-PELI3 antibody (AAA19930).Overlay histogram showing HepG2 cells stained with AAA19930 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI3 Antibody (AAA19930, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of PELI3 using anti-PELI3 antibody (AAA19930).PELI3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI3 Antibody (AAA19930) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI3 antigen affinity purified polyclonal antibody (#AAA19930) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PELI3 at approximately 51 kDa. The expected band size for PELI3 is at 51 kDa.)

PELI3, Polyclonal Antibody (Cat# AAA19930)

Full Name

Anti-PELI3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-Dynamin 1 antibody (AAA19160).Overlay histogram showing U20S cells stained with AAA19160 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1 Antibody (AAA19160,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Dynamin 1, Polyclonal Antibody (Cat# AAA19160)

Full Name

Anti-Dynamin 1 Picoband antibody

Gene Names

DNM1; DNM; EIEE31

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ILDR1 antibody (AAA19928).Overlay histogram showing HepG2 cells stained with AAA19928 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ILDR1 Antibody (AAA19928, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of ILDR1 using anti-ILDR1 antibody (AAA19928).ILDR1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ILDR1 Antibody (AAA19928) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ILDR1 Antibody (AAA19928) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ILDR1 Antibody (AAA19928) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILDR1 antigen affinity purified polyclonal antibody (#AAA19928) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ILDR1 at approximately 37 kDa. The expected band size for ILDR1 is at 40 kDa.)

IDH3A, Polyclonal Antibody (Cat# AAA19928)

Full Name

Anti-IDH3A Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

PPAR alpha, Polyclonal Antibody (Cat# AAA31448)

Full Name

Phospho-PPAR alpha (Ser12) Antibody

Gene Names

PPARA; PPAR; NR1C1; hPPAR; PPARalpha

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (83%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using ALYREF Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)

ALYREF, Polyclonal Antibody (Cat# AAA30677)

Full Name

ALYREF Rabbit Polyclonal Antibody

Gene Names

ALYREF; ALY; BEF; REF; THOC4; ALY/REF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GLRX at dilution of 1:100. Blue: DAPI for nuclear staining.)

GLRX, Polyclonal Antibody (Cat# AAA29654)

Full Name

GLRX Antibody

Gene Names

GLRX; GRX; GRX1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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PPARGC1A, Polyclonal Antibody (Cat# AAA10967)

Full Name

PPARGC1A Antibody

Gene Names

PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry-paraffin (IHC-p), Immunofluroescence (IF)

Purity

PPARGC1A antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ASPSCR1 antibody. Blue: DAPI for nuclear staining.)

ASPSCR1, Polyclonal Antibody (Cat# AAA28119)

Full Name

ASPSCR1

Gene Names

ASPSCR1; TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using PKM1-specific Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

PKM1-specific, Polyclonal Antibody (Cat# AAA28375)

Full Name

PKM1-specific Rabbit pAb

Gene Names

PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

HNRNPA0, Polyclonal Antibody (Cat# AAA31447)

Full Name

Phospho-HNRNPA0 (Ser84) Antibody

Gene Names

HNRNPA0; HNRPA0

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CTSE antibody.)

CTSE, Polyclonal Antibody (Cat# AAA10712)

Full Name

CTSE Polyclonal Antibody

Gene Names

CTSE; CATE

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Detection of mouse RPA32 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

RPA32, Polyclonal Antibody (Cat# AAA23770)

Full Name

Rabbit anti-RPA32 Antibody, Affinity Purified

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Antigen Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of LGALS3BP using anti-LGALS3BP antibody (AAA19163).LGALS3BP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGALS3BP Antibody (AAA19163) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

LGALS3BP, Polyclonal Antibody (Cat# AAA19163)

Full Name

Anti-LGALS3BP Picoband Antibody

Reactivity

Human, Mouse
No cross reactivity with other proteins.

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Pricing

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WB (Western Blot) (Detection of mouse PCNA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

PCNA, Polyclonal Antibody (Cat# AAA23771)

Full Name

Rabbit anti-PCNA Antibody, Affinity Purified

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF)

Purity

Antigen Affinity Purified

Pricing

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TOMM20, Polyclonal Antibody (Cat# AAA28123)

Full Name

TOMM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

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SFPQ, Polyclonal Antibody (Cat# AAA10716)

Full Name

SFPQ Polyclonal Antibody

Gene Names

SFPQ; PSF; POMP100; PPP1R140

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of OSGIN1 using anti-OSGIN1 antibody (AAA19937).OSGIN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of OSGIN1 and Tubulin beta using anti-OSGIN1 antibody (AAA19937) and anti-Tubulin beta antibody (M05613-4).OSGIN1 and Tubulin beta was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human T-47D whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSGIN1 antigen affinity purified polyclonal antibody (#AAA19937) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OSGIN1 at approximately 53 kDa. The expected band size for OSGIN1 is at 61 kDa.)

OSGIN1, Polyclonal Antibody (Cat# AAA19937)

Full Name

Anti-OSGIN1 Antibody Picoband

Gene Names

OSGIN1; BDGI; OKL38

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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GLUD1, Polyclonal Antibody (Cat# AAA23521)

Full Name

GLUD1 antibody - N-terminal region

Gene Names

GLUD1; GDH; GDH1; GLUD

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using LLGL1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

LLGL1, Polyclonal Antibody (Cat# AAA28385)

Full Name

LLGL1 Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

HSPBAP1, Polyclonal Antibody (Cat# AAA29153)

Full Name

HSPBAP1 Polyclonal Antibody

Gene Names

HSPBAP1; PASS1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cell using MCM7 antibody. Blue: DAPI for nuclear staining.)

MCM7, Polyclonal Antibody (Cat# AAA29665)

Full Name

MCM7 Antibody

Gene Names

MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using RIPK1/RIP Rabbit pAb.)

RIPK1, Polyclonal Antibody (Cat# AAA30689)

Full Name

RIPK1 Rabbit Polyclonal Antibody

Gene Names

RIPK1; RIP; RIP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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INCENP, Polyclonal Antibody (Cat# AAA31457)

Full Name

Phospho-INCENP (Thr59) Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

Alpha-Tubulin, Polyclonal Antibody (Cat# AAA29919)

Full Name

Alpha-Tubulin Antibody

Gene Names

TUBA4A; TUBA1; H2-ALPHA

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Peptide affinity purified

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of GSTM3 using anti-GSTM3 antibody (AAA19168).GSTM3 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSTM3 Antibody (AAA19168) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GSTM3, Polyclonal Antibody (Cat# AAA19168)

Full Name

Anti-GSTM3 Picoband antibody

Gene Names

GSTM3; GST5; GSTB; GTM3; GSTM3-3

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Pricing

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AMH, Polyclonal Antibody (Cat# AAA29664)

Full Name

AMH Antibody

Gene Names

AMH; MIF; MIS

Reactivity

Human

Applications

Immunohistochemistry (IHC)

Purity

Antigen affinity purification.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZEB2 antibody at dilution of 1:100 (40x lens).)

ZEB2, Polyclonal Antibody (Cat# AAA28133)

Full Name

ZEB2 Polyclonal Antibody

Gene Names

ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

KCNK9, Polyclonal Antibody (Cat# AAA28901)

Full Name

KCNK9 (TASK-3) Polyclonal Antibody

Gene Names

KCNK9; KT3.2; TASK3; K2p9.1; TASK-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Eotaxin-3, Polyclonal Antibody (Cat# AAA29157)

Full Name

Eotaxin-3 Polyclonal Antibody

Gene Names

CCL26; IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (WB Suggested Anti-CHAF1B Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Liver)

CHAF1B, Polyclonal Antibody (Cat# AAA23522)

Full Name

CHAF1B antibody - N-terminal region

Gene Names

CHAF1B; CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Detection of human and mouse ICLN by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICLN antibody AAA23778 (lot AAA23778-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

ICLN, Polyclonal Antibody (Cat# AAA23778)

Full Name

Rabbit anti-ICLN Antibody, Affinity Purified

Gene Names

CLNS1A; CLCI; ICln; CLNS1B

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Antigen Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

DLL3, Polyclonal Antibody (Cat# AAA26850)

Full Name

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)

Gene Names

DLL3; SCDO1

Reactivity

Human

Applications

Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)

Other applications not tested.

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using GLRX antibody. Blue: DAPI for nuclear staining.)

GLRX, Polyclonal Antibody (Cat# AAA28130)

Full Name

GLRX Polyclonal Antibody

Gene Names

GLRX; GRX; GRX1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

GABA A Receptor alpha4, Polyclonal Antibody (Cat# AAA28898)

Full Name

GABA A Receptor alpha4 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NAA10 at 1:1000 dilution.)

NAA10, Polyclonal Antibody (Cat# AAA30685)

Full Name

NAA10 Rabbit Polyclonal Antibody

Gene Names

NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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HMGB1, Polyclonal Antibody (Cat# AAA10718)

Full Name

HMGB1 Polyclonal Antibody

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence of MAGEA4 inHeLa cells with MAGEA4 antibody at 20 μg/ml.Red: MAGEA4 Antibody (8191)Blue: DAPI staining)

MAGEA4, Polyclonal Antibody (Cat# AAA10974)

Full Name

MAGEA4 Antibody

Gene Names

MAGEA4; CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

MAGEA4 antibody is affinity chromatography purified via peptide column.

Pricing

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MTUS1, Polyclonal Antibody (Cat# AAA23518)

Full Name

MTUS1 antibody - middle region

Gene Names

MTUS1; ATBP; ATIP; ICIS; MP44; ATIP3; MTSG1

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of F9 cells using TET2 Rabbit pAb (AAA28132) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

TET2, Polyclonal Antibody (Cat# AAA28132)

Full Name

TET2 Polyclonal Antibody

Gene Names

TET2; MDS; KIAA1546

Applications

Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Ghrelin Receptor, Polyclonal Antibody (Cat# AAA28900)

Full Name

Ghrelin Receptor Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IL-4, Polyclonal Antibody (Cat# AAA29156)

Full Name

IL-4 Polyclonal Antibody

Gene Names

IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human embryo brain tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

MTR4, Polyclonal Antibody (Cat# AAA29668)

Full Name

MTR4 antibody

Gene Names

SKIV2L2; Dob1; Mtr4; fSAP118; KIAA0052

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using PGD antibody.)

PGD, Polyclonal Antibody (Cat# AAA30692)

Full Name

PGD Rabbit Polyclonal Antibody

Gene Names

PGD; 6PGD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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MCM2, Polyclonal Antibody (Cat# AAA31460)

Full Name

Phospho-MCM2 (Ser27) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IP (Immunoprecipitation) (Figure 7. Immunoprecipitating GLIPR2 in U251 whole cell lysate .Western blot analysis of GLIPR2 using anti-GLIPR2 antibody (AAA19941).Lane 1: U251 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-GLIPR2 antibody in U251 whole cell lysate.Lane 3: anti-GLIPR2 antibody (2ug) + U251 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (AAA19941) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIPR2 Antibody (AAA19941, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GLIPR2 using anti-GLIPR2 antibody (AAA19941).GLIPR2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 8: mouse brain tissue lysates,Lane 9: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (#AAA19941) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLIPR2 at approximately 17 kDa. The expected band size for GLIPR2 is at 17 kDa.)

GLIPR2, Polyclonal Antibody (Cat# AAA19941)

Full Name

Anti-GLIPR2 Antibody Picoband

Gene Names

GLIPR2; GAPR1; GAPR-1; C9orf19

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using INTS6 antibody. Blue: DAPI for nuclear staining.)

INTS6, Polyclonal Antibody (Cat# AAA28131)

Full Name

INTS6 Polyclonal Antibody

Gene Names

INTS6; HDB; INT6; DBI-1; DDX26; DICE1; DDX26A; Notchl2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of SH-SY5Y cells using anti-IPO9 antibody (AAA19940).Overlay histogram showing SH-SY5Y cells stained with AAA19940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPO9 Antibody (AAA19940, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of IPO9 using anti-IPO9 antibody (AAA19940).IPO9 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO9 antigen affinity purified polyclonal antibody (#AAA19940) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IPO9 at approximately 120 kDa. The expected band size for IPO9 is at 120 kDa.)

IPO9, Polyclonal Antibody (Cat# AAA19940)

Full Name

Anti-IPO9 Antibody Picoband

Gene Names

IPO9; Imp9

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using c-Jun Polyclonal antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

c-Jun, Polyclonal Antibody (Cat# AAA22244)

Full Name

c-Jun Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PELI2 antibody (AAA19939).Overlay histogram showing THP-1 cells stained with AAA19939 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI2 Antibody (AAA19939, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PELI2 using anti-PELI2 antibody (AAA19939).PELI2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PELI2 Antibody (AAA19939) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Ramos whole cell lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat testis tissue lysates,Lane 5: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI2 antigen affinity purified polyclonal antibody (#AAA19939) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PELI2 at approximately 50 kDa. The expected band size for PELI2 is at 46 kDa.)

PELI2, Polyclonal Antibody (Cat# AAA19939)

Full Name

Anti-PELI2 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-SGCE Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysateSGCE is supported by BioGPS gene expression data to be expressed in 721_B)

SGCE, Polyclonal Antibody (Cat# AAA23523)

Full Name

SGCE antibody - N-terminal region

Gene Names

SGCE; ESG; DYT11; epsilon-SG

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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Viewing 1850-1900 of 3252 product results

PELI3, Polyclonal Antibody (Cat# AAA19930)

Dynamin 1, Polyclonal Antibody (Cat# AAA19160)

IDH3A, Polyclonal Antibody (Cat# AAA19928)

PPAR alpha, Polyclonal Antibody (Cat# AAA31448)

ALYREF, Polyclonal Antibody (Cat# AAA30677)

GLRX, Polyclonal Antibody (Cat# AAA29654)

PPARGC1A, Polyclonal Antibody (Cat# AAA10967)

ASPSCR1, Polyclonal Antibody (Cat# AAA28119)

PKM1-specific, Polyclonal Antibody (Cat# AAA28375)

HNRNPA0, Polyclonal Antibody (Cat# AAA31447)

CTSE, Polyclonal Antibody (Cat# AAA10712)

RPA32, Polyclonal Antibody (Cat# AAA23770)

LGALS3BP, Polyclonal Antibody (Cat# AAA19163)

PCNA, Polyclonal Antibody (Cat# AAA23771)

TOMM20, Polyclonal Antibody (Cat# AAA28123)

SFPQ, Polyclonal Antibody (Cat# AAA10716)

OSGIN1, Polyclonal Antibody (Cat# AAA19937)

GLUD1, Polyclonal Antibody (Cat# AAA23521)

LLGL1, Polyclonal Antibody (Cat# AAA28385)

HSPBAP1, Polyclonal Antibody (Cat# AAA29153)

MCM7, Polyclonal Antibody (Cat# AAA29665)

RIPK1, Polyclonal Antibody (Cat# AAA30689)

INCENP, Polyclonal Antibody (Cat# AAA31457)

Alpha-Tubulin, Polyclonal Antibody (Cat# AAA29919)

GSTM3, Polyclonal Antibody (Cat# AAA19168)

AMH, Polyclonal Antibody (Cat# AAA29664)

ZEB2, Polyclonal Antibody (Cat# AAA28133)

KCNK9, Polyclonal Antibody (Cat# AAA28901)

Eotaxin-3, Polyclonal Antibody (Cat# AAA29157)

CHAF1B, Polyclonal Antibody (Cat# AAA23522)

ICLN, Polyclonal Antibody (Cat# AAA23778)

DLL3, Polyclonal Antibody (Cat# AAA26850)

GLRX, Polyclonal Antibody (Cat# AAA28130)

GABA A Receptor alpha4, Polyclonal Antibody (Cat# AAA28898)

NAA10, Polyclonal Antibody (Cat# AAA30685)

HMGB1, Polyclonal Antibody (Cat# AAA10718)

MAGEA4, Polyclonal Antibody (Cat# AAA10974)

MTUS1, Polyclonal Antibody (Cat# AAA23518)

TET2, Polyclonal Antibody (Cat# AAA28132)

Ghrelin Receptor, Polyclonal Antibody (Cat# AAA28900)

IL-4, Polyclonal Antibody (Cat# AAA29156)

MTR4, Polyclonal Antibody (Cat# AAA29668)

PGD, Polyclonal Antibody (Cat# AAA30692)

MCM2, Polyclonal Antibody (Cat# AAA31460)

GLIPR2, Polyclonal Antibody (Cat# AAA19941)

INTS6, Polyclonal Antibody (Cat# AAA28131)

IPO9, Polyclonal Antibody (Cat# AAA19940)

c-Jun, Polyclonal Antibody (Cat# AAA22244)

PELI2, Polyclonal Antibody (Cat# AAA19939)

SGCE, Polyclonal Antibody (Cat# AAA23523)

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