Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human embryo brain tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human liver cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded rat liver tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human kidney cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat heart tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat brain tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MTR4 antibody.)

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MTR4, Polyclonal Antibody (Cat# AAA29668)

Full Name

MTR4 antibody

Gene Names

SKIV2L2; Dob1; Mtr4; fSAP118; KIAA0052

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using PGD antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human mammary cancer using PGD antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using PGD Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using PGD Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse heart using PGD antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PGD Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PGD antibody.)

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PGD, Polyclonal Antibody (Cat# AAA30692)

Full Name

PGD Rabbit Polyclonal Antibody

Gene Names

PGD; 6PGD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of MCM2 (Phospho-Ser27) using serum treated COLO205 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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MCM2, Polyclonal Antibody (Cat# AAA31460)

Full Name

Phospho-MCM2 (Ser27) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IP (Immunoprecipitation) (Figure 7. Immunoprecipitating GLIPR2 in U251 whole cell lysate .Western blot analysis of GLIPR2 using anti-GLIPR2 antibody (AAA19941).Lane 1: U251 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-GLIPR2 antibody in U251 whole cell lysate.Lane 3: anti-GLIPR2 antibody (2ug) + U251 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (AAA19941) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIPR2 Antibody (AAA19941, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GLIPR2 using anti-GLIPR2 antibody (AAA19941).GLIPR2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLIPR2 Antibody (AAA19941) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U20S whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 8: mouse brain tissue lysates,Lane 9: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (#AAA19941) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLIPR2 at approximately 17 kDa. The expected band size for GLIPR2 is at 17 kDa.)

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GLIPR2, Polyclonal Antibody (Cat# AAA19941)

Full Name

Anti-GLIPR2 Antibody Picoband

Gene Names

GLIPR2; GAPR1; GAPR-1; C9orf19

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using MAOB antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAOB antibody.)

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MAOB, Polyclonal Antibody (Cat# AAA29672)

Full Name

MAOB Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using HSP90AB1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat intestine using HSP90AB1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HSP90AB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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HSP90AB1, Polyclonal Antibody (Cat# AAA10729)

Full Name

HSP90AB1 Polyclonal Antibody

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$255/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-P4HB Antibody Titration: 0.5ug/mlPositive Control: HepG2 cell lysateP4HB is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)

WB (Western Blot) (Host: RabbitTarget Name: P4HBSample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: P4HBSample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: P4HBSample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: P4HBSample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Human kidney)

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P4HB, Polyclonal Antibody (Cat# AAA23529)

Full Name

P4HB antibody - N-terminal region

Gene Names

P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZEB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using ZEB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using ZEB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using ZEB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using ZEB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ZEB2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ZEB2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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ZEB2, Polyclonal Antibody (Cat# AAA28133)

Full Name

ZEB2 Polyclonal Antibody

Gene Names

ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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KCNK9, Polyclonal Antibody (Cat# AAA28901)

Full Name

KCNK9 (TASK-3) Polyclonal Antibody

Gene Names

KCNK9; KT3.2; TASK3; K2p9.1; TASK-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Eotaxin-3, Polyclonal Antibody (Cat# AAA29157)

Full Name

Eotaxin-3 Polyclonal Antibody

Gene Names

CCL26; IMAC; TSC-1; MIP-4a; SCYA26; MIP-4alpha

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using INTS6 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using INTS6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using INTS6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using INTS6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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INTS6, Polyclonal Antibody (Cat# AAA28131)

Full Name

INTS6 Polyclonal Antibody

Gene Names

INTS6; HDB; INT6; DBI-1; DDX26; DICE1; DDX26A; Notchl2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of SH-SY5Y cells using anti-IPO9 antibody (AAA19940).Overlay histogram showing SH-SY5Y cells stained with AAA19940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPO9 Antibody (AAA19940, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Hela cells using anti-IPO9 antibody (AAA19940).Overlay histogram showing Hela cells stained with AAA19940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPO9 Antibody (AAA19940, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of IPO9 using anti-IPO9 antibody (AAA19940).IPO9 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO9 antigen affinity purified polyclonal antibody (#AAA19940) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IPO9 at approximately 120 kDa. The expected band size for IPO9 is at 120 kDa.)

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IPO9, Polyclonal Antibody (Cat# AAA19940)

Full Name

Anti-IPO9 Antibody Picoband

Gene Names

IPO9; Imp9

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using c-Jun Polyclonal antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using c-Jun Polyclonal antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A431 cells using c-Jun Polyclonal antibody at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using c-Jun Polyclonal antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of MCF7 cells using c-Jun Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of Rat kidney using c-Jun Polyclonal Antibody at 1:1000 dilution.)

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c-Jun, Polyclonal Antibody (Cat# AAA22244)

Full Name

c-Jun Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (VIM antibody - C-terminal region validated by WB using MCF-7 whole cell lysates at 1.0ug/ml.)

WB (Western Blot) (VIM antibody - C-terminal region validated by WB using 1. Human NT-2 cells (60ug) at 2ug/ml.)

WB (Western Blot) (Host: RabbitTarget Name: VIMSample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: VIMSample Tissue: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Immunohistochemistry with NT-2 cell line tissue)

IHC (Immunohistochemistry) (Immunohistochemistry with NT-2 cell line tissue)

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VIM, Polyclonal Antibody (Cat# AAA23531)

Full Name

VIM antibody - C-terminal region

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of mouse brain, using GTF2I antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GTF2I antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GTF2I antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using GTF2I antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody.)

WB (Western Blot) (Western blot analysis of extracts of BT-474 cells, using GTF2I antibody.)

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GTF2I, Polyclonal Antibody (Cat# AAA30699)

Full Name

GTF2I Rabbit Polyclonal Antibody

Gene Names

GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of for immunohistochemistry.)

Application Data (All lanes: at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (039288 at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (All lanes: at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Western Blot analysis of OPN-a/b (arrow))

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OPN-a/b, Polyclonal Antibody (Cat# AAA26861)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 490)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), FLISA

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

$1,055/0.1 mL $2,045/2x0.1 mL $2,980/3x0.1 mL $3,865/4x0.1 mL $4,515/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen using PPP4C antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung using PPP4C antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer using PPP4C antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer using PPP4C antibody at dilution of 1:200 (400x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PPP4C antibody.)

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PPP4C, Polyclonal Antibody (Cat# AAA29677)

Full Name

PPP4C Antibody

Gene Names

PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CETN3 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CETN3 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using CETN3 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CETN3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using CETN3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CETN3 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver damage using CETN3 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CETN3 at 1:1000 dilution.)

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CETN3, Polyclonal Antibody (Cat# AAA30701)

Full Name

CETN3 Rabbit Polyclonal Antibody

Gene Names

CETN3; CEN3; CDC31

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of AXL (Phospho-Tyr702) using 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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AXL, Polyclonal Antibody (Cat# AAA31469)

Full Name

Phospho-AXL (Tyr702) Antibody

Gene Names

AXL; UFO; JTK11

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using HDAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HDAC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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HDAC2, Polyclonal Antibody (Cat# AAA10734)

Full Name

HDAC2 Polyclonal Antibody

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of for immunohistochemistry.)

Application Data (All lanes: at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (039288 at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (All lanes: at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Western Blot analysis of OPN-a/b (arrow))

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OPN-a/b, Polyclonal Antibody (Cat# AAA26865)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (PE)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), FLISA

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

$1,055/0.2 mL $2,045/2x0.2 mL $2,980/3x0.2 mL $3,865/4x0.2 mL $4,515/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using JMJD6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using JMJD6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using JMJD6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using JMJD6 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using JMJD6 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse liver, using JMJD6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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JMJD6, Polyclonal Antibody (Cat# AAA28145)

Full Name

JMJD6 Polyclonal Antibody

Gene Names

JMJD6; PSR; PTDSR; PTDSR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from 293T, Hela cell line (from left to right), using GLS Antibody (C-term). AAA28657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody (C-term). AAA28657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from human brain and mouse brain tissue lysate (from left to right), using GLS Antibody (C-term). AAA28657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IF (Immunofluorescence) (Fluorescent image of Hela cells stained with XAF1 GLS Antibody (C-term). AAA28657 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IF (Immunofluorescence) (Fluorescent image of Hela cells stained with XAF1 GLS Antibody (C-term). AAA28657 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H.kidney section using GLS Antibody (C-term). AAA28657 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

WB (Western Blot) (Western blot analysis of lysates from human brain tissue and Hela cell line (from left to right), using GLS Antibody (C-term). AAA28657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

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GLS, Polyclonal Antibody (Cat# AAA28657)

Full Name

GLS Antibody (C-term)

Gene Names

GLS; GAC; GAM; KGA; GLS1; AAD20

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.05 mL $480/0.2 mL $925/2x0.2 mL $1,345/3x0.2 mL $1,740/4x0.2 mL $1,975/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MDC1 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using MDC1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MDC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using MDC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 at dilution of 1:100 (40x lens).)

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MDC1, Polyclonal Antibody (Cat# AAA30705)

Full Name

MDC1 Rabbit Polyclonal Antibody

Gene Names

MDC1; NFBD1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NCF4 antibody.Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using NCF4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using NCF4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using NCF4 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NCF4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NCF4/p40-phox antibody (AAA10738) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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NCF4, Polyclonal Antibody (Cat# AAA10738)

Full Name

NCF4 Polyclonal Antibody

Gene Names

NCF4; NCF; P40PHOX; SH3PXD4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

IHC (Immunohistochemistry) (Figure 7. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (AAA19952).GOLGA7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GOLGA7 Antibody (AAA19952) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

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GOLGA7, Polyclonal Antibody (Cat# AAA19952)

Full Name

Anti-GOLGA7 Antibody

Gene Names

GOLGA7; GCP16; GOLGA7A; HSPC041; GOLGA3AP1

Reactivity

Human

Applications

Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using UBQLN4 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using UBQLN4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using UBQLN4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using UBQLN4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using UBQLN4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using UBQLN4 Polyclonal Antibody at 1:1000 dilution.)

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UBQLN4, Polyclonal Antibody (Cat# AAA22256)

Full Name

UBQLN4 Polyclonal Antibody

Gene Names

UBQLN4; A1U; A1Up; UBIN; CIP75; C1orf6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ULK4 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using ULK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric using ULK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using ULK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using ULK4 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ULK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

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ULK4, Polyclonal Antibody (Cat# AAA28144)

Full Name

ULK4

Gene Names

ULK4; FAM7C1; REC01035

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse colon using MUC2 Rabbit pAb (AAA28400) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat rectum using MUC2 Rabbit pAb (AAA28400) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using MUC2 Rabbit pAb (AAA28400) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using MUC2 Rabbit pAb (AAA28400) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MUC2 antibody (AAA28400) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of HT-29 cells, using MUC2 antibody (AAA28400) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

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MUC2, Polyclonal Antibody (Cat# AAA28400)

Full Name

MUC2 Rabbit pAb

Gene Names

MUC2; MLP; SMUC; MUC-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-NUBPL antibody (AAA19948).Overlay histogram showing MCF-7 cells stained with AAA19948 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUBPL Antibody (AAA19948, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NUBPL using anti-NUBPL antibody (AAA19948).NUBPL was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUBPL Antibody (AAA19948) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human T-47D whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human Colo320 whole cell lysates,Lane 6: human MCF-7 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUBPL antigen affinity purified polyclonal antibody (#AAA19948) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUBPL at approximately 34 kDa. The expected band size for NUBPL is at 34 kDa.)

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NUBPL, Polyclonal Antibody (Cat# AAA19948)

Full Name

Anti-NUBPL Antibody Picoband

Gene Names

NUBPL; IND1; huInd1; C14orf127

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of ID1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry) (ID1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (ID1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).)

WB (Western Blot) (ID1 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).)

WB (Western Blot) (Western blot analysis of lysates from HepG2, MCF-7, U-2OS cell line (from left to right), using ID1 Antibody (Center). AAA28652 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

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ID1, Polyclonal Antibody (Cat# AAA28652)

Full Name

ID1 Antibody (Center)

Gene Names

ID1; ID; bHLHb24

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Annexin A3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1, 000 dilution. Positive control�� Lane1: Siha Lane2: Hela Lane3: Rat spleen tissue Lane4: Rat liver tissue Lane5: Mouse lung tissue)

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Annexin A3, Polyclonal Antibody (Cat# AAA29932)

Full Name

Annexin A3 Antibody

Gene Names

ANXA3; ANX3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using DDB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using DDB1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using DDB1 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDB1 Antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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DDB1, Polyclonal Antibody (Cat# AAA10733)

Full Name

DDB1 Polyclonal Antibody

Gene Names

DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using ATP1B1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using ATP1B1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATP1B1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

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ATP1B1, Polyclonal Antibody (Cat# AAA28138)

Full Name

ATP1B1 Polyclonal Antibody

Gene Names

ATP1B1; ATP1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (PROX-1-S514 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (PROX-1-S514 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PROX-1-S514 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysates from HepG2 cell line and mouse liver tissue lysate(from left to right), using PROX-1 Antibody (S514). AAA28650 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (All lanes : Anti-PROX-1-S514 Antibody at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: mouse liver lysatesLane 3: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-PROX-1-S514 Antibody at 1:2000 dilutionLane 1: SW620 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-PROX-1-S514 Antibody at 1:1000 dilutionLane 1: HepG2 whole cell lysatesLane 2: SH-SY5Y whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 83 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

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PROX-1-S514, Polyclonal Antibody (Cat# AAA28650)

Full Name

PROX-1-S514 Antibody

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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Kv11.3, Polyclonal Antibody (Cat# AAA28906)

Full Name

Kv11.3 Polyclonal Antibody

Gene Names

KCNH7; ERG3; HERG3; Kv11.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Apelin, Polyclonal Antibody (Cat# AAA29162)

Full Name

Apelin Polyclonal Antibody

Gene Names

APLN; APEL; XNPEP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining DOCK4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DOCK4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

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DOCK4, Polyclonal Antibody (Cat# AAA29930)

Full Name

DOCK4 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of rat kidney using CDH16 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CDH16 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using CDH16 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using CDH16 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CDH16 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CDH16 at 1:1000 dilution.)

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CDH16, Polyclonal Antibody (Cat# AAA30698)

Full Name

CDH16 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining TROP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-TROP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-TROP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TROP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TROP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TROP2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TROP2 on A431 cell lysate using anti-TROP2 antibody at 1/500 dilution.)

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TROP2, Polyclonal Antibody (Cat# AAA29936)

Full Name

TROP2 Antibody

Gene Names

TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse skeletal muscle using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human kidney using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using PVALB Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using PVALB Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse skeletal muscle, using PVALB antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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PVALB, Polyclonal Antibody (Cat# AAA10737)

Full Name

PVALB Polyclonal Antibody

Gene Names

PVALB; D22S749

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Rat brain using C9orf72 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using C9orf72 Polyclonal Antibody at 1:1000 dilution.)

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C9orf72, Polyclonal Antibody (Cat# AAA22257)

Full Name

C9orf72 Polyclonal Antibody

Gene Names

C9orf72; ALSFTD; FTDALS; FTDALS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-UNC84A Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: UNC84ASample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: UNC84ASample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SUN1Sample Type: Jurkat Whole Cell lysatesAntibody Dilution: 3ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SUN1Sample Tissue: Human HCT116Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SUN1Sample Tissue: Human HCT116 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Sample Type :Mouse C2C12 cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit-Alexa Fluor 488Secondary Antibody Dilution :1:500Color/Signal Descriptions :UNC84a: Green DAPI: BlueGene Name :UNC84ASubmitted by :Dr. David Razafsky, Washington University in Saint Louis)

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UNC84A, Polyclonal Antibody (Cat# AAA23537)

Full Name

UNC84A antibody - N-terminal region

Gene Names

SUN1; UNC84A

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Daudi cells with Tle6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Tle6 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Tle6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Tle6 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution. Positive control�� Lane1: Mouse ovary tissue Lane2: HepG2)

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Tle6, Polyclonal Antibody (Cat# AAA29935)

Full Name

Tle6 Antibody

Gene Names

TLE6; GRG6

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified.

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

IHC (Immunohistochemistry) (Figure 7. IHC analysis of IRF2BPL using anti-IRF2BPL antibody (AAA19950).IRF2BPL was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human MCF-7whole cell lysates,Lane 4: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2BPL antigen affinity purified polyclonal antibody (#AAA19950) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF2BPL at approximately 95 kDa. The expected band size for IRF2BPL is at 83 kDa.)

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IRF2BPL, Polyclonal Antibody (Cat# AAA19950)

Full Name

Anti-IRF2BPL Antibody Picoband

Gene Names

IRF2BPL; EAP1; C14orf4

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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AP-1, Polyclonal Antibody (Cat# AAA28921)

Full Name

AP-1 (phospho Ser63) Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using AMBRA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using AMBRA1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FLJ20294 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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AMBRA1, Polyclonal Antibody (Cat# AAA10746)

Full Name

AMBRA1 Polyclonal Antibody

Gene Names

AMBRA1; DCAF3; WDR94

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

WB (Western Blot) (The cell lysate derived HepG2 was in 12% SDS-PAGE, transferred onto NC membrane, and immunoblotted by Rabbit anti PLAC1 (AAA14074) at 1:500. An immunoreactive major band was observed at ~22 kDa.)

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PLAC1, Polyclonal Antibody (Cat# AAA14074)

Full Name

Rabbit anti PLAC1 Polyclonal Antibody

Gene Names

PLAC1; CT92; OOSP2L

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

Purity

The Rabbit IgG is purified by Epitope Affinity Purification.

Pricing

$360/0.1 mg $690/2x0.1 mg $1,000/3x0.1 mg $1,295/4x0.1 mg $1,375/5x0.1 mg

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WB (Western Blot)

WB (Western Blot)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

IHC (Immunohistochemistry)

FCM (Flow Cytometry)

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FEN1, Polyclonal Antibody (Cat# AAA19194)

Full Name

Anti-FEN1 Antibody

Gene Names

FEN1; MF1; RAD2; FEN-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of Daudi cells using anti-RBM15B antibody (AAA19962).Overlay histogram showing Daudi cells stained with AAA19962 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15B Antibody (AAA19962, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of RBM15B using anti-RBM15B antibody (AAA19962).RBM15B was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 10. IF analysis of RBM15B using anti-RBM15B antibody (AAA19962).RBM15B was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of RBM15B using anti-RBM15B antibody (AAA19962).RBM15B was detected in a paraffin-embedded section of human undifferentiated carcinoma of pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat C6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15B antigen affinity purified polyclonal antibody (#AAA19962) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBM15B at approximately 110 kDa. The expected band size for RBM15B is at 97 kDa.)

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RBM15B, Polyclonal Antibody (Cat# AAA19962)

Full Name

Anti-RBM15B Antibody Picoband

Gene Names

RBM15B; OTT3; HUMAGCGB

Reactivity

Human, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.