Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1750-1800 of 3176 product results
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using Caspase-3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Caspase-3, Polyclonal Antibody (Cat# AAA22200)
Full Name
Caspase-3 Polyclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
ZBTB48, Polyclonal Antibody (Cat# AAA29927)
Full Name
ZBTB48 Antibody
Gene Names
ZBTB48; HKR3; ZNF855; pp9964
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse testis cells using DDX4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
DDX4, Polyclonal Antibody (Cat# AAA22255)
Full Name
DDX4 Polyclonal Antibody
Gene Names
DDX4; VASA
Reactivity
Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/50.)
S100B, Polyclonal Antibody (Cat# AAA29687)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human Mouse Rat
Applications
IHC
Purity
Antigen affinity purification.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Hela cells using anti-Caspase-9/CASP9 antibody (AAA19215).Overlay histogram showing Hela cells stained with AAA19215 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9/CASP9 Antibody (AAA19215, 1 μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Caspase-9/CASP9, Polyclonal Antibody (Cat# AAA19215)
Full Name
Anti-Caspase-9/CASP9 Antibody
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Reactivity
Human
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-Serum Response Factor/SRF antibody (AAA19221).Overlay histogram showing U251 cells stained with AAA19221 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serum Response Factor/SRF Antibody (AAA19221, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Serum Response Factor/SRF, Polyclonal Antibody (Cat# AAA19221)
Full Name
Anti-Serum Response Factor/SRF Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of THP-1 cells using anti-BCKDK antibody (AAA19589).Overlay histogram showing THP-1 cells stained with AAA19589 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCKDK Antibody (AAA19589, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BCKDK, Polyclonal Antibody (Cat# AAA19589)
Full Name
Anti-BCKDK Antibody Picoband
Gene Names
BCKDK; BDK; BCKDKD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of 293T cells using anti-TRMT6 antibody (AAA19634).Overlay histogram showing 293T cells stained with AAA19634 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRMT6 Antibody (AAA19634, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TRMT6, Polyclonal Antibody (Cat# AAA19634)
Full Name
Anti-TRMT6 Antibody Picoband
Gene Names
TRMT6; GCD10; CGI-09; Gcd10p
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U937 cells using anti-GNG7 antibody (AAA19335).Overlay histogram showing U937 cells stained with AAA19335 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG7 Antibody (AAA19335, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG7, Polyclonal Antibody (Cat# AAA19335)
Full Name
Anti-GNG7 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(FOXN2 Antibody (N-term) (AAA28719) western blot analysis in NCI-H292, Jurkat, K562 cell line lysates (35ug/lane).This demonstrates the FOXN2 antibody detected the FOXN2 protein (arrow).)
FOXN2, Polyclonal Antibody (Cat# AAA28719)
Full Name
FOXN2 Antibody (N-term)
Gene Names
FOXN2; HTLF
Reactivity
Human
Applications
WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26887)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using CEBPE antibody.)
CEBPE, Polyclonal Antibody (Cat# AAA29704)
Full Name
CEBPE Antibody
Gene Names
CEBPD; CELF; CRP3; C/EBP-delta; NF-IL6-beta
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
CD83, Polyclonal Antibody (Cat# AAA28988)
Full Name
CD83 Polyclonal Antibody
Gene Names
CD83; BL11; HB15
Reactivity
Human
Applications
WB, IHC-P
Pricing
Application Data
(Anti-mApple Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
mApple, Polyclonal Antibody (Cat# AAA13883)
Full Name
anti-mApple
Reactivity
Reacts with transfected cells proteins
Applications
WB, IF, IHC-P, IHC-F, IEM
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
Tenascin-C, Polyclonal Antibody (Cat# AAA29295)
Full Name
Tenascin-C Polyclonal Antibody
Gene Names
TNC; GP; JI; TN; HXB; GMEM; TN-C; 150-225
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-RAB27B antibody (AAA19825).Overlay histogram showing PC-3 cells stained with AAA19825 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB27B Antibody (AAA19825, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RAB27B, Polyclonal Antibody (Cat# AAA19825)
Full Name
Anti-RAB27B Antibody Picoband
Gene Names
RAB27B; C25KG
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PDPK1, Polyclonal Antibody (Cat# AAA11643)
Full Name
Anti-PDPK1 Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
MMP-13, Polyclonal Antibody (Cat# AAA29070)
Full Name
MMP-13 Polyclonal Antibody
Gene Names
MMP13; CLG3; MANDP1
Reactivity
Human
Applications
WB, IHC-P, IF
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31388)
Full Name
Phospho-p27 Kip1 (Ser178) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
GH, Polyclonal Antibody (Cat# AAA29359)
Full Name
GH Polyclonal Antibody
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Reactivity
Human
Applications
IHC-P
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using EDF1 antibody at dilution of 1:100 (40x lens).)
EDF1, Polyclonal Antibody (Cat# AAA10692)
Full Name
EDF1 Polyclonal Antibody
Gene Names
EDF1; MBF1; EDF-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)
Granzyme B, Polyclonal Antibody (Cat# AAA29390)
Full Name
Granzyme B Polyclonal Antibody
Gene Names
GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TPPP, Polyclonal Antibody (Cat# AAA31436)
Full Name
Phospho-TPPP (Ser18) Antibody
Gene Names
TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (89%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (89%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of IRF2BPL using anti-IRF2BPL antibody (AAA19950).IRF2BPL was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human MCF-7whole cell lysates,Lane 4: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2BPL antigen affinity purified polyclonal antibody (#AAA19950) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF2BPL at approximately 95 kDa. The expected band size for IRF2BPL is at 83 kDa.)
IRF2BPL, Polyclonal Antibody (Cat# AAA19950)
Full Name
Anti-IRF2BPL Antibody Picoband
Gene Names
IRF2BPL; EAP1; C14orf4
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ID1, Polyclonal Antibody (Cat# AAA28652)
Full Name
ID1 Antibody (Center)
Gene Names
ID1; ID; bHLHb24
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Carbonic Anhydrase 13/CA13 antibody (AAA19326).Overlay histogram showing CACO-2 cells stained with AAA19326 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (AAA19326, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Carbonic Anhydrase 13/CA13, Polyclonal Antibody (Cat# AAA19326)
Full Name
Anti-Carbonic Anhydrase 13/CA13 Antibody
Gene Names
CA13; CAXIII
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of GHITM using anti-GHITM antibody (AAA19621).GHITM was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GHITM Antibody (AAA19621) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GHITM, Polyclonal Antibody (Cat# AAA19621)
Full Name
Anti-GHITM Antibody Picoband
Gene Names
GHITM; DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with PUMA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
PUMA, Polyclonal Antibody (Cat# AAA29955)
Full Name
PUMA Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Ku-70, Polyclonal Antibody (Cat# AAA29224)
Full Name
Ku-70 Polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Vimentin, Polyclonal Antibody (Cat# AAA22322)
Full Name
Vimentin Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47699(GNA12 Antibody) at dilution 1/20.(Original magnification: 200))
GNA12, Polyclonal Antibody (Cat# AAA29764)
Full Name
GNA12 Antibody
Gene Names
GNA12; RMP; gep; NNX3
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Antigen affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Nucleophosmin, Polyclonal Antibody (Cat# AAA31308)
Full Name
Phospho-Nucleophosmin (Ser125) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
JAM-A, Polyclonal Antibody (Cat# AAA29269)
Full Name
JAM-A Polyclonal Antibody
Gene Names
F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1
Reactivity
Human, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using MKI67 antibody.)
MKI67, Polyclonal Antibody (Cat# AAA28242)
Full Name
MKI67 Polyclonal Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Jurkat cells using CASP3 antibody at dilution of 1:100. Jurkat cells treated by Etoposide 25uM etoposide for 5 hours (left). Blue: DAPI for nuclear staining.)
CASP3, Polyclonal Antibody (Cat# AAA28248)
Full Name
CASP3 Polyclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
IL-1F8, Polyclonal Antibody (Cat# AAA29281)
Full Name
IL-1F8 Polyclonal Antibody
Gene Names
IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
Reactivity
Human
Applications
WB, IHC-P
Pricing
Application Data
anti-Human IgM, Polyclonal Antibody (Cat# AAA14450)
Full Name
Affinity Purified Goat anti-Human IgM
Reactivity
Human
Purity
Antigen Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of U-87MG cells using 3ug MAPK14 antibody. Western blot was performed from the immunoprecipitate using MAPK14 antibody at a dilition of 1:1000.)
MAPK14, Polyclonal Antibody (Cat# AAA28274)
Full Name
MAPK14 Polyclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using FLNA at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
FLNA, Polyclonal Antibody (Cat# AAA30641)
Full Name
FLNA Rabbit Polyclonal Antibody
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-HNRPUL1 Antibody Titration: 0.25ug/mlELISA Titer: 1:312500Positive Control: Raji cell lysateHNRNPUL1 is supported by BioGPS gene expression data to be expressed in Raji)
HNRPUL1, Polyclonal Antibody (Cat# AAA23483)
Full Name
HNRPUL1 antibody - C-terminal region
Gene Names
HNRNPUL1; E1BAP5; E1B-AP5; HNRPUL1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
IF, IP, IHC, WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-POSTN AntibodyTitration: 1 ug/mlPositive Control: 293T cells lysate)
POSTN, Polyclonal Antibody (Cat# AAA23493)
Full Name
POSTN antibody - middle region
Gene Names
POSTN; PN; OSF2; OSF-2; PDLPOSTN
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SLC39A5 Antibody Titration: 0.5ug/mlPositive Control: HepG2 cell lysate)
SLC39A5, Polyclonal Antibody (Cat# AAA23515)
Full Name
SLC39A5 antibody - N-terminal region
Gene Names
SLC39A5; ZIP5; MYP24; LZT-Hs7
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
Application Data
(Dilution: WB 1:1000-2000, IHC 1:100-200)
SLC12A4, Polyclonal Antibody (Cat# AAA28903)
Full Name
SLC12A4 Polyclonal Antibody
Gene Names
SLC12A4; KCC1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat brain using HACE1 at dilution of 1:100 (40x lens).)
HACE1, Polyclonal Antibody (Cat# AAA30714)
Full Name
HACE1 Rabbit Polyclonal Antibody
Gene Names
HACE1; SPPRS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Cdk4, Polyclonal Antibody (Cat# AAA29176)
Full Name
Cdk4 Polyclonal Antibody
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast using PRPF3 Antibody at dilution of 1:100 (40x lens).)
PRPF3, Polyclonal Antibody (Cat# AAA28150)
Full Name
PRPF3 Polyclonal Antibody
Gene Names
PRPF3; PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U87 cells using anti-GRB10 antibody (AAA19244).Overlay histogram showing U87 cells stained with AAA19244 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB10 Antibody (AAA19244,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GRB10, Polyclonal Antibody (Cat# AAA19244)
Full Name
Anti-GRB10 Antibody
Gene Names
GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of Cbx8 using anti-Cbx8 antibody (AAA19304).Cbx8 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cbx8 Antibody (AAA19304) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
Cbx8, Polyclonal Antibody (Cat# AAA19304)
Full Name
Anti-Cbx8 Antibody
Gene Names
CBX8; PC3; RC1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
RPA32/RPA2, Polyclonal Antibody (Cat# AAA31304)
Full Name
Phospho-RPA32/RPA2 (Ser4/Ser8) Antibody
Gene Names
RPA2; REPA2; RPA32
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CDCP1, Polyclonal Antibody (Cat# AAA29206)
Full Name
CDCP1 Polyclonal Antibody
Gene Names
CDCP1; CD318; TRASK; SIMA135
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.