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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1750-1800 of 10000 product results
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000)
CYFIP2, Polyclonal Antibody (Cat# AAA29330)
Full Name
CYFIP2 Polyclonal Antibody
Gene Names
CYFIP2; PIR121
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: HumanSkin cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human IFI16 Antibody;Second Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))
Interferon Gamma Inducible Protein 16, Polyclonal Antibody (Cat# AAA20929)
Full Name
Polyclonal Antibody to Interferon Gamma Inducible Protein 16 (IFI16)
Gene Names
IFI16; PYHIN2; IFNGIP1
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U20S cells using anti-TMEM87A antibody (AAA19997).Overlay histogram showing U20S cells stained with AAA19997 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM87A Antibody (AAA19997, 1ug/1x106 cells) for 30 min at 20 degree C. PE conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
TMEM87A, Polyclonal Antibody (Cat# AAA19997)
Full Name
Anti-TMEM87A Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-RPL36AL AntibodyTitration: 1.0 ug/mlPositive Control: Jurkat Whole CellRPL36AL is supported by BioGPS gene expression data to be expressed in Jurkat)
RPL36AL, Polyclonal Antibody (Cat# AAA23596)
Full Name
RPL36AL Antibody - C-terminal region
Gene Names
RPL36AL; RPL36A
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry-Paraffin)
(Immunochemical staining of rat in rat kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
FH/Fumarate Hydratase, Polyclonal Antibody (Cat# AAA27777)
Full Name
Anti-FH/Fumarate Hydratase Antibody, Rabbit Polyclonal
Reactivity
Human, Mouse, Rat, Danre, Pig, Macfa
(Species predicted to react based on 100% sequence homology)
(Species predicted to react based on 100% sequence homology)
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using SULT2A1 Antibody at dilution of 1:200 (40x lens).)
SULT2A1, Polyclonal Antibody (Cat# AAA28271)
Full Name
SULT2A1 Polyclonal Antibody
Gene Names
SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)
Cdk6, Polyclonal Antibody (Cat# AAA29351)
Full Name
Cdk6 Polyclonal Antibody
Gene Names
CDK6; PLSTIRE
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using CD146/MCAM Rabbit pAb.)
MCAM, Polyclonal Antibody (Cat# AAA30619)
Full Name
MCAM Rabbit Polyclonal Antibody
Gene Names
MCAM; CD146; MUC18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using BRSK1 at dilution of 1:100 (40x lens).)
BRSK1, Polyclonal Antibody (Cat# AAA30660)
Full Name
BRSK1 Rabbit Polyclonal Antibody
Gene Names
BRSK1; hSAD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18850 at dilution 1:100)
Kynurenine 3-monooxygenase, Polyclonal Antibody (Cat# AAA18850)
Full Name
Rabbit anti-human Kynurenine 3-monooxygenase polyclonal Antibody(KMO)
Gene Names
KMO; dJ317G22.1
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of RAW264.7 cells using anti-APP-1/PABPC4 antibody (AAA19592).Overlay histogram showing RAW264.7 cells stained with AAA19592 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APP-1/PABPC4 Antibody (AAA19592, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
APP-1/PABPC4, Polyclonal Antibody (Cat# AAA19592)
Full Name
Anti-APP-1/PABPC4 Antibody Picoband
Gene Names
PABPC4; APP1; APP-1; PABP4; iPABP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-GNA11 AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Brain)
GNA11, Polyclonal Antibody (Cat# AAA23594)
Full Name
GNA11 antibody - C-terminal region
Gene Names
GNA11; FBH; FBH2; FHH2; HHC2; GNA-11; HYPOC2
Reactivity
Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26891)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffinembedded Human tonsil tissue using POU6F2 Antibody at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: 200))
POU6F2, Polyclonal Antibody (Cat# AAA29650)
Full Name
POU6F2 Antibody
Gene Names
POU6F2; WT5; WTSL; RPF-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
EPAS-1, Polyclonal Antibody (Cat# AAA29219)
Full Name
EPAS-1 Polyclonal Antibody
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using p70 S6 Kinase 1 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA28344)
Full Name
p70 S6 Kinase 1 Rabbit pAb
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
gp91-phox, Polyclonal Antibody (Cat# AAA29238)
Full Name
gp91-phox Polyclonal Antibody
Gene Names
CYBB; CGD; NOX2; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
ICC (Immunocytochemistry)
(Anti-SHC antibody, AAA11580, ICCICC: A549 Cell)
SHC, Polyclonal Antibody (Cat# AAA11580)
Full Name
Anti-SHC antibody
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(ATG16L1/ATG16L Antibody-Human Colon, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))
ATG16L1/ATG16L, Polyclonal Antibody (Cat# AAA21359)
Full Name
ATG16L1/ATG16L Rabbit anti-Human Polyclonal Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using ELP3 antibody.)
ELP3, Polyclonal Antibody (Cat# AAA30580)
Full Name
ELP3 Polyclonal Antibody
Gene Names
ELP3; KAT9
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using HSP90AB1 antibody. Blue: DAPI for nuclear staining.)
Hsp90beta, Polyclonal Antibody (Cat# AAA28434)
Full Name
Hsp90beta Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Dilution: IF: 1:50-200 IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)
PTEN, Polyclonal Antibody (Cat# AAA29328)
Full Name
PTEN Polyclonal Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
p73, Polyclonal Antibody (Cat# AAA29227)
Full Name
p73 Polyclonal Antibody
Gene Names
TP73; P73
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunocytochemistry: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Rock-1, Polyclonal Antibody (Cat# AAA29112)
Full Name
Rock-1 Polyclonal Antibody
Gene Names
ROCK1; ROCK-I; P160ROCK
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Ub, Polyclonal Antibody (Cat# AAA28851)
Full Name
Ub (Acetyl Lys27) Polyclonal Antibody
Gene Names
UBA52; L40; CEP52; RPL40; HUBCEP52
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of extracts of mouse liver, using TRPA1 at 1:1000 dilution.)
TRPA1, Polyclonal Antibody (Cat# AAA30709)
Full Name
TRPA1 Rabbit Polyclonal Antibody
Gene Names
TRPA1; FEPS; ANKTM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using CSNK2A2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
CSNK2A2, Polyclonal Antibody (Cat# AAA30616)
Full Name
CSNK2A2 Rabbit Polyclonal Antibody
Gene Names
CSNK2A2; CK2A2; CSNK2A1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26855)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (Azide free) (HRP)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
IHC, EIA, WB
Purity
Purified by Protein G Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Human Colorectal cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human FLNC AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Filamin C Gamma, Polyclonal Antibody (Cat# AAA20854)
Full Name
Polyclonal Antibody to Filamin C Gamma (FLNC)
Gene Names
FLNC; ABPA; ABPL; FLN2; MFM5; MPD4; RCM5; CMH26; ABP-280; ABP280A
Reactivity
Human
Applications
WB, IHC
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using ASB3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
ASB3, Polyclonal Antibody (Cat# AAA22266)
Full Name
ASB3 Polyclonal Antibody
Gene Names
ASB3; ASB-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of THP-1 cells using anti-IMPDH1 antibody (AAA19500).Overlay histogram showing THP-1 cells stained with AAA19500 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMPDH1 Antibody (AAA19500, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IMPDH1, Polyclonal Antibody (Cat# AAA19500)
Full Name
Anti-IMPDH1 Antibody Picoband
Gene Names
IMPDH1; IMPD; RP10; IMPD1; LCA11; IMPDH-I; sWSS2608
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 9. IHC analysis of SMIM8 using anti-SMIM8 antibody (AAA20009).SMIM8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
SMIM8, Polyclonal Antibody (Cat# AAA20009)
Full Name
Anti-SMIM8 Antibody
Gene Names
SMIM8; C6orf162; dJ102H19.2
Reactivity
Human
Applications
Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26892)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
DPP7, Polyclonal Antibody (Cat# AAA29148)
Full Name
DPP7 Polyclonal Antibody
Gene Names
DPP7; QPP; DPP2; DPPII; DPP7
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)
ZP2, Polyclonal Antibody (Cat# AAA30586)
Full Name
ZP2 Rabbit Polyclonal Antibody
Gene Names
ZP2; ZPA; Zp-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Recoverin/RCVRN using anti-Recoverin/RCVRN antibody (AAA19878).Recoverin/RCVRN was detected in a paraffin-embedded section of human uterus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Recoverin/RCVRN Antibody (AAA19878) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Recoverin/RCVRN antigen affinity purified polyclonal antibody (#AAA19878) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Recoverin/RCVRN at approximately 26kDa. The expected band size for Recoverin/RCVRN is at 23 kDa.)
Recoverin/RCVRN, Polyclonal Antibody (Cat# AAA19878)
Full Name
Anti-Recoverin/RCVRN Antibody Picoband
Gene Names
RCVRN; RCV1
Reactivity
Human
Applications
WB, IHC, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry-FUT4 Polyclonal Antibody)
FUT4, Polyclonal Antibody (Cat# AAA28294)
Full Name
FUT4 Polyclonal Antibody
Gene Names
FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using IRE1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IRE1, Polyclonal Antibody (Cat# AAA28358)
Full Name
IRE1 Rabbit pAb
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (OTUD1) Polyclonal Antibody, Unconjugated (bs-17563R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
OTUD1, Polyclonal Antibody (Cat# AAA28819)
Full Name
OTUD1 Polyclonal Antibody
Gene Names
OTUD1; DUBA7; OTDC1
Reactivity
Rat, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Cerberus, Polyclonal Antibody (Cat# AAA29248)
Full Name
Cerberus Polyclonal Antibody
Gene Names
CER1; DAND4
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody.)
FGD1, Polyclonal Antibody (Cat# AAA29820)
Full Name
FGD1 Polyclonal Antibody
Gene Names
FGD1; AAS; FGDY; MRXS16; ZFYVE3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ATG7 antibody (AAA19732).Overlay histogram showing HepG2 cells stained with AAA19732 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG7 Antibody (AAA19732, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATG7, Polyclonal Antibody (Cat# AAA19732)
Full Name
Anti-ATG7 Antibody Picoband
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A-549 cells using CEBPG.)
CEBPG, Polyclonal Antibody (Cat# AAA30687)
Full Name
CEBPG Rabbit Polyclonal Antibody
Gene Names
CEBPG; GPE1BP; IG/EBP-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA31132 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
EFNB2, Polyclonal Antibody (Cat# AAA31132)
Full Name
EFNB2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using HPSE Antibody at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG)
heparanase, Polyclonal Antibody (Cat# AAA15907)
Full Name
Rabbit anti-human heparanase polyclonal Antibody
Gene Names
HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
Reactivity
Human, Mouse; Other species are not tested. Please decide the specificity by homology.
Applications
Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).)
DDB1, Polyclonal Antibody (Cat# AAA10733)
Full Name
DDB1 Polyclonal Antibody
Gene Names
DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-NSF antibody (AAA19222).Overlay histogram showing U937 cells stained with AAA19222 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSF Antibody (AAA19222, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NSF, Polyclonal Antibody (Cat# AAA19222)
Full Name
Anti-NSF Antibody
Gene Names
NSF; SKD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of RH35 cells using anti-TORC1/CRTC1 antibody (AAA19251).Overlay histogram showing RH35 cells stained with AAA19251 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TORC1/CRTC1 Antibody (AAA19251, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TORC1/CRTC1, Polyclonal Antibody (Cat# AAA19251)
Full Name
Anti-TORC1/CRTC1 Antibody
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Angptl1, Polyclonal Antibody (Cat# AAA29235)
Full Name
Angptl1 Polyclonal Antibody
Gene Names
ANGPTL1; ANG3; ARP1; AngY; ANGPT3; UNQ162; dJ595C2.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody at dilution of 1:200 .)
AFG3L2, Polyclonal Antibody (Cat# AAA30577)
Full Name
AFG3L2 Polyclonal Antibody
Gene Names
AFG3L2; SCA28; SPAX5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.