Polyclonal Antibodies

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GAPDH, Polyclonal Antibody (Cat# AAA13657)

Full Name

Goat anti-GAPDH (Internal) Antibody

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog

Applications

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

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WB (Western Blot) (Figure 6. Western blot analysis of ABCE1 using anti-ABCE1 antibody (AAA19808).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human U20S whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: mouse 4T1 whole cell lysates,Lane 5: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (AAA19808) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.)

IF (Immunofluorescence) (Figure 4. IF analysis of ABCE1 using anti-ABCE1 antibody (AAA19808).ABCE1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABCE1 Antibody (AAA19808) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABCE1 Antibody (AAA19808) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A431 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse 4T1 whole cell lysates,Laen 8: mouse NIH/3T3 whole cell lysate.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (#AAA19808) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.)

ABCE1, Polyclonal Antibody (Cat# AAA19808)

Full Name

Anti-ABCE1 Antibody Picoband

Gene Names

ABCE1; RLI; OABP; RLI1; ABC38; RNS4I; RNASEL1; RNASELI

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (TPM2 Antibody-Immunohistochemistry of paraffin-embedded rat testis tissue.)

TPM2, Polyclonal Antibody (Cat# AAA21344)

Full Name

TPM2 Rabbit anti-Human Polyclonal Antibody

Gene Names

TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EGLN1+EGLN2 antibody.)

EGLN1/EGLN2, Polyclonal Antibody (Cat# AAA29792)

Full Name

EGLN1/EGLN2 Polyclonal Antibody

Gene Names

EGLN1; HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ZNF581 antibody at dilution of 1:100 .)

ZNF581, Polyclonal Antibody (Cat# AAA30560)

Full Name

ZNF581 Polyclonal Antibody

Gene Names

ZNF581; HSPC189

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

CRF-RI, Polyclonal Antibody (Cat# AAA29273)

Full Name

CRF-RI Polyclonal Antibody

Gene Names

CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRHR1f; CRF-R-1; CRH-R-1; CRH-R1h

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-NSUN2 antibody (AAA19802).Overlay histogram showing 293T cells stained with AAA19802 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSUN2 Antibody (AAA19802, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of NSUN2 using anti-NSUN2 antibody (AAA19802).NSUN2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN2 antigen affinity purified polyclonal antibody (#AAA19802) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NSUN2 at approximately 100 kDa. The expected band size for NSUN2 is at 100 kDa.)

NSUN2, Polyclonal Antibody (Cat# AAA19802)

Full Name

Anti-NSUN2 Antibody Picoband

Gene Names

NSUN2; MISU; MRT5; SAKI; TRM4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (TP53/p53 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

TP53/p53, Polyclonal Antibody (Cat# AAA21340)

Full Name

TP53/p53 Rabbit anti-Human Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Immunoaffinity purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

ERK 1/2, Polyclonal Antibody (Cat# AAA29020)

Full Name

ERK 1/2 Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

SRY, Polyclonal Antibody (Cat# AAA29276)

Full Name

SRY Polyclonal Antibody

Gene Names

SRY; TDF; TDY; SRXX1; SRXY1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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mTOR, Polyclonal Antibody (Cat# AAA30548)

Full Name

mTOR Polyclonal Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

ZCCHC11/PAPD3, Polyclonal Antibody (Cat# AAA13653)

Full Name

Goat anti-ZCCHC11/PAPD3 Antibody

Gene Names

ZCCHC11; TUT4; PAPD3

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Applications

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

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WB (Western Blot) (Host: RabbitTarget Name: GLI2Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 2.5ug/mLPeptide Concentration: 4.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 6%-18%)

GLI2, Polyclonal Antibody (Cat# AAA23395)

Full Name

GLI2 antibody - middle region

Gene Names

GLI2; CJS; HPE9; PHS2; THP1; THP2

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

FSHR, Polyclonal Antibody (Cat# AAA29027)

Full Name

FSHR Polyclonal Antibody

Gene Names

FSHR; LGR1; ODG1; FSHRO

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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Smad5, Polyclonal Antibody (Cat# AAA31331)

Full Name

Phospho-Smad5 (Ser463/Ser465) Antibody

Gene Names

SMAD5; DWFC; JV5-1; MADH5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using AAA18535 at dilution of 1:100)

COLGALT2, Polyclonal Antibody (Cat# AAA18535)

Full Name

COLGALT2 Antibody

Gene Names

COLGALT2; C1orf17; GLT25D2

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen Affinity Purified

Pricing

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WB (Western Blot) (Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)

GOLPH2/GOLM1, Polyclonal Antibody (Cat# AAA27751)

Full Name

Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal

Gene Names

GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

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WB (Western Blot) (Anti-SLC10A1 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SLC10A1, Polyclonal Antibody (Cat# AAA28775)

Full Name

SLC10A1 Antibody (C-term)

Gene Names

SLC10A1; NTCP

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAX antibody.)

BAX, Polyclonal Antibody (Cat# AAA29799)

Full Name

BAX Polyclonal Antibody

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of JK cells using anti-NF45/ILF2 antibody (AAA19816).Overlay histogram showing JK cells stained with AAA19816 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF45/ILF2 Antibody (AAA19816, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AAA19816) and anti-Beta Tubulin antibody (M01857-3).NF45/ILF2 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NF45/ILF2 Antibody (AAA19816) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat C6 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF45/ILF2 antigen affinity purified polyclonal antibody (#AAA19816) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NF45/ILF2 at approximately 43 kDa. The expected band size for NF45/ILF2 is at 43 kDa.)

NF45/ILF2, Polyclonal Antibody (Cat# AAA19816)

Full Name

Anti-NF45/ILF2 Antibody Picoband

Gene Names

ILF2; NF45; PRO3063

Reactivity

Human, Mouse, Rat, Monkey

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

GRIN2, Polyclonal Antibody (Cat# AAA29033)

Full Name

GRIN2 Polyclonal Antibody

Gene Names

GPRIN2; GRIN2; KIAA0514

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

RSAD2, Polyclonal Antibody (Cat# AAA29289)

Full Name

RSAD2 Polyclonal Antibody

Gene Names

RSAD2; cig5; vig1; cig33; 2510004L01Rik

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

CANX/Calnexin, Polyclonal Antibody (Cat# AAA21354)

Full Name

CANX/Calnexin Rabbit anti-Human Polyclonal Antibody

Gene Names

CANX; CNX; P90; IP90

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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WB (Western Blot) (WB Suggested Anti-PAX4 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)

PAX4, Polyclonal Antibody (Cat# AAA23402)

Full Name

PAX4 antibody - middle region

Gene Names

PAX4; KPD; MODY9

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

TEAD1, Polyclonal Antibody (Cat# AAA28264)

Full Name

TEAD1 Polyclonal Antibody

Gene Names

TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

GPR40, Polyclonal Antibody (Cat# AAA29032)

Full Name

GPR40 Polyclonal Antibody

Gene Names

FFAR1; FFA1R; GPR40; GPCR40

Reactivity

Human, Monkey

Applications

Western Blot (WB), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

COL5A2, Polyclonal Antibody (Cat# AAA29288)

Full Name

COL5A2 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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beta Catenin, Polyclonal Antibody (Cat# AAA29797)

Full Name

beta Catenin Polyclonal Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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CAMLG, Polyclonal Antibody (Cat# AAA30565)

Full Name

CAMLG Polyclonal Antibody

Gene Names

CAMLG; CAML

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using PRKCB Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

PRKCB, Polyclonal Antibody (Cat# AAA30564)

Full Name

PRKCB Polyclonal Antibody

Gene Names

PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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HP1 alpha, Polyclonal Antibody (Cat# AAA31332)

Full Name

Phospho-HP1 alpha (Ser92) Antibody

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (ANXA1/Annexin A1 Antibody-Immunohistochemical analysis of Annexin A1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)

ANXA1/Annexin A1, Polyclonal Antibody (Cat# AAA21346)

Full Name

ANXA1/Annexin A1 Rabbit anti-Human Polyclonal (Internal) Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Mouse, Human, Monkey

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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IF (Immunofluorescence) (Figure 7. IF analysis of RFX1 using anti-RFX1 antibody (AAA19814).RFX1 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RFX1 using anti-RFX1 antibody (AAA19814).RFX1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX1 antigen affinity purified polyclonal antibody (#AAA19814) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFX1 at approximately 135 kDa. The expected band size for RFX1 is at 105 kDa.)

RFX1, Polyclonal Antibody (Cat# AAA19814)

Full Name

Anti-RFX1 Antibody Picoband

Gene Names

RFX1; EFC; RFX

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified.

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IHC (Immunohistchemistry) (HMG-CoA Reductase/HMGCR Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

HMG-CoA Reductase/HMGCR, Polyclonal Antibody (Cat# AAA21345)

Full Name

HMG-CoA Reductase/HMGCR Rabbit anti-Human Polyclonal Antibody

Gene Names

HMGCR; LDLCQ3

Reactivity

Mouse, Rat, Human

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Immunoaffinity purified

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MCM2, Polyclonal Antibody (Cat# AAA31329)

Full Name

Phospho-MCM2 (Ser41) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Histone H3, Polyclonal Antibody (Cat# AAA29038)

Full Name

Histone H3 Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

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IF (Immunofluorescence) (Immunofluorescence analysis of human breast cancer using COL1A1 antibody.)

COL1A1, Polyclonal Antibody (Cat# AAA29806)

Full Name

COL1A1 Polyclonal Antibody

Gene Names

COL1A1; OI4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Rac1/cdc42, Polyclonal Antibody (Cat# AAA31342)

Full Name

Rac1/cdc42 Antibody

Gene Names

RAC1; MIG5; Rac-1; TC-25; p21-Rac1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

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IHC (Immunohistchemistry) (Anti-NFkB p100 Picoband antibody, AAA11631-6.JPGIHC(P): Rat Intestine Tissue)

Nuclear factor NF-kappa-B p100 subunit, Polyclonal Antibody (Cat# AAA11631)

Full Name

Anti-NFkB p100/p52 Antibody

Gene Names

NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Western Blot (WB), Immunohistochemistry (IHC-P)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody)

KLF2, Polyclonal Antibody (Cat# AAA13679)

Full Name

Goat anti-KLF2 (aa248-260) Antibody

Reactivity

Human

Applications

Pep-ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody at dilution of 1:200 .)

AFG3L2, Polyclonal Antibody (Cat# AAA30577)

Full Name

AFG3L2 Polyclonal Antibody

Gene Names

AFG3L2; SCA28; SPAX5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Histone H3, Polyclonal Antibody (Cat# AAA31345)

Full Name

Acetyl-Histone H3 (Lys14) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SNW1 antibody.)

SNW1, Polyclonal Antibody (Cat# AAA29810)

Full Name

SNW1 Polyclonal Antibody

Gene Names

SNW1; Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

Histone H2A.X, Polyclonal Antibody (Cat# AAA29036)

Full Name

Histone H2A.X Polyclonal Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

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IHC (Immunohistchemistry) (AAA31084 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)