Polyclonal Antibodies

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MBD2 antibody at dilution of 1:100 (40x lens).)

MBD2, Polyclonal Antibody (Cat# AAA10669)

Full Name

MBD2 Polyclonal Antibody

Gene Names

MBD2; DMTase; NY-CO-41

Reactivity

Human

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-LARG/ARHGEF12 antibody (AAA19877).Overlay histogram showing HepG2 cells stained with AAA19877 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARG/ARHGEF12 Antibody (AAA19877, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ARHGEF12 using anti-ARHGEF12 antibody (AAA19877) and anti-Beta Tubulin antibody (M01857-3).ARHGEF12 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat testis tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (#AAA19877) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.)

LARG/ARHGEF12, Polyclonal Antibody (Cat# AAA19877)

Full Name

Anti-LARG/ARHGEF12 Antibody Picoband

Gene Names

ARHGEF12; LARG; PRO2792

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Cleaved-Notch 2, Polyclonal Antibody (Cat# AAA28837)

Full Name

Cleaved-Notch 2 (D1733) Polyclonal Antibody

Gene Names

NOTCH2; hN2; AGS2; HJCYS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

Gremlin-2, Polyclonal Antibody (Cat# AAA29352)

Full Name

Gremlin-2 Polyclonal Antibody

Gene Names

GREM2; PRDC; DAND3; CKTSF1B2

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts from Mouse spleen, using Cytochrome c Antibody. The lane on the left was treated with blocking peptide.)

Cytochrome c, Polyclonal Antibody (Cat# AAA31144)

Full Name

Cytochrome c Antibody

Gene Names

CYCS; CYC; HCS; THC4

Reactivity

Human, Mouse, Rat, Monkey
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)

Full Name

Anti-ATP citrate lyase Antibody

Gene Names

ACLY; ACL; ATPCL; CLATP

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF416 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ZNF416, Polyclonal Antibody (Cat# AAA22185)

Full Name

ZNF416 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

Cdk6, Polyclonal Antibody (Cat# AAA29351)

Full Name

Cdk6 Polyclonal Antibody

Gene Names

CDK6; PLSTIRE

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

EGFR, Polyclonal Antibody (Cat# AAA31399)

Full Name

Phospho-EGFR (Tyr998) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (WB Suggested Anti-PDLIM5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that PDLIM5 is expressed in HepG2)

PDLIM5, Polyclonal Antibody (Cat# AAA23464)

Full Name

PDLIM5 antibody - N-terminal region

Gene Names

PDLIM5; L9; ENH; LIM; ENH1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SNW1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

SNW1, Polyclonal Antibody (Cat# AAA22182)

Full Name

SNW1 Polyclonal Antibody

Gene Names

SNW1; Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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SOX4, Polyclonal Antibody (Cat# AAA23462)

Full Name

SOX4 antibody - N-terminal region

Gene Names

SOX4; EVI16

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Cleaved-PARP-1, Polyclonal Antibody (Cat# AAA28838)

Full Name

Cleaved-PARP-1 (D214) Polyclonal Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

EGF, Polyclonal Antibody (Cat# AAA29350)

Full Name

EGF Polyclonal Antibody

Gene Names

EGF; URG; HOMG4

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using CTNNA2 antibody.)

CTNNA2, Polyclonal Antibody (Cat# AAA30630)

Full Name

CTNNA2 Rabbit Polyclonal Antibody

Gene Names

CTNNA2; CAPR; CTNR; CAP-R; CT114

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)

TXN, Polyclonal Antibody (Cat# AAA22183)

Full Name

TXN Polyclonal Antibody

Gene Names

TXN; TRX; TRDX; TRX1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

p53, Polyclonal Antibody (Cat# AAA28842)

Full Name

p53 (Di Methyl Lys370) Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

PKA Ibeta reg, Polyclonal Antibody (Cat# AAA29098)

Full Name

PKA Ibeta reg Polyclonal Antibody

Gene Names

PRKAR1B; PRKAR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

CAMKK1/2, Polyclonal Antibody (Cat# AAA31402)

Full Name

Phospho-CAMKK1/2 (Ser458/Ser495) Antibody

Gene Names

CAMKK1; CAMKKA

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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HNRNPK, Polyclonal Antibody (Cat# AAA10667)

Full Name

HNRNPK Polyclonal Antibody

Gene Names

HNRNPK; CSBP; TUNP; HNRPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using AAA18859 at dilution of 1:100)

Cellular tumor antigen p53, Polyclonal Antibody (Cat# AAA18859)

Full Name

Rabbit anti-human Cellular tumor antigen p53 polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-PFKP antibody (AAA19883).Overlay histogram showing MCF-7 cells stained with AAA19883 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKP Antibody (AAA19883, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PFKP using anti-PFKP antibody (AAA19883).PFKP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKP Antibody (AAA19883) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKP Antibody (AAA19883) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKP Antibody (AAA19883) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKP Antibody (AAA19883) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKP Antibody (AAA19883) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKP antigen affinity purified polyclonal antibody (#AAA19883) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 80-86 kDa.)

PFKP, Polyclonal Antibody (Cat# AAA19883)

Full Name

Anti-PFKP Antibody Picoband

Gene Names

PFKP; PFKF; PFK-C

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Tau, Polyclonal Antibody (Cat# AAA29353)

Full Name

Tau Polyclonal Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Retinoblastoma, Polyclonal Antibody (Cat# AAA31401)

Full Name

Phospho-Retinoblastoma (Ser788) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (92%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Patched, Polyclonal Antibody (Cat# AAA29092)

Full Name

Patched Polyclonal Antibody

Gene Names

PTCH1; PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody at dilution of 1:100 (40x lens).)

ITCH, Polyclonal Antibody (Cat# AAA28315)

Full Name

[KO Validated] ITCH Rabbit pAb

Gene Names

ITCH; AIF4; AIP4; NAPP1; dJ468O1.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CD146/MCAM Rabbit pAb.)

MCAM, Polyclonal Antibody (Cat# AAA30619)

Full Name

MCAM Rabbit Polyclonal Antibody

Gene Names

MCAM; CD146; MUC18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

p27 Kip1, Polyclonal Antibody (Cat# AAA31387)

Full Name

Phospho-p27 Kip1 (Thr157) Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-BCAT2 antibody (AAA19868).Overlay histogram showing MCF-7 cells stained with AAA19868 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT2 Antibody (AAA19868, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of BCAT2 using anti-BCAT2 antibody (AAA19868).BCAT2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (#AAA19868) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 38-42 kDa.)

BCAT2, Polyclonal Antibody (Cat# AAA19868)

Full Name

Anti-BCAT2 Antibody Picoband

Gene Names

BCAT2; BCAM; BCT2; PP18; BCATM

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

PSMD2, Polyclonal Antibody (Cat# AAA29103)

Full Name

PSMD2 Polyclonal Antibody

Gene Names

PSMD2; S2; P97; Rpn1; TRAP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

GH, Polyclonal Antibody (Cat# AAA29359)

Full Name

GH Polyclonal Antibody

Gene Names

GH1; GH; GHN; GH-N; hGH-N; IGHD1B

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CD44, Polyclonal Antibody (Cat# AAA28336)

Full Name

CD44 Rabbit pAb

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Rab 34, Polyclonal Antibody (Cat# AAA29104)

Full Name

Rab 34 Polyclonal Antibody

Gene Names

RAB34; RAH; RAB39

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

CD16, Polyclonal Antibody (Cat# AAA29360)

Full Name

CD16 Polyclonal Antibody

Gene Names

FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

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FCM (Flow Cytometry) (Flow cytometric analysis of NIH-3T3 cells with PDGF Receptor beta antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

PDGF beta, Polyclonal Antibody (Cat# AAA29872)

Full Name

PDGF Receptor beta Antibody

Gene Names

PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HSPB1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HSPB1, Polyclonal Antibody (Cat# AAA22193)

Full Name

(KO Validated) HSPB1 Polyclonal Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

ompA, Polyclonal Antibody (Cat# AAA27060)

Full Name

Rabbit anti-Escherichia coli O157:H7 ompA Polyclonal Antibody

Reactivity

Escherichia coli O157:H7

Applications

ELISA (EIA), Western Blot (WB)

Purity

Protein G

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IHC (Immunohistchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Reg IV, Polyclonal Antibody (Cat# AAA29108)

Full Name

Reg IV Polyclonal Antibody

Gene Names

REG4; GISP; RELP; REG-IV

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using TGFB1 Rabbit pAb.)

TGFB1, Polyclonal Antibody (Cat# AAA30644)

Full Name

TGFB1 Rabbit Polyclonal Antibody

Gene Names

TGFB1; CED; LAP; DPD1; TGFB; TGFbeta

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

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FRS2, Polyclonal Antibody (Cat# AAA31412)

Full Name

Phospho-FRS2 (Tyr196) Antibody

Gene Names

FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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WB (Western Blot) (Figure 7. Western blot analysis of PLS1 using anti-PLS1 antibody (AAA19893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

IF (Immunofluorescence) (Figure 5. IF analysis of PLS1 using anti-PLS1 antibody (AAA19893).PLS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: rat small intestine tissue lysates,Lane 6: rat stomach tissue lysates,Lane 7: mouse small intestine tissue lysates,Lane 8: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (#AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

PLS1, Polyclonal Antibody (Cat# AAA19893)

Full Name

Anti-PLS1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CHCHD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CHCHD2, Polyclonal Antibody (Cat# AAA28333)

Full Name

[KO Validated] CHCHD2 Rabbit pAb

Gene Names

CHCHD2; C7orf17

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

GROalpha, Polyclonal Antibody (Cat# AAA29357)

Full Name

GROalpha Polyclonal Antibody

Gene Names

CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with M6PR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.)

IGF2R/M6PR, Polyclonal Antibody (Cat# AAA29869)

Full Name

IGF2R/M6PR Antibody

Gene Names

IGF2R; MPR1; MPRI; CD222; CIMPR; M6P-R

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

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JAK3, Polyclonal Antibody (Cat# AAA31405)

Full Name

Phospho-JAK3 (Tyr981) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Rag A/B, Polyclonal Antibody (Cat# AAA29106)

Full Name

Rag A/B Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using MTIF2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)