Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of C6 cells using anti-BCL11B antibody (AAA19750).Overlay histogram showing C6 cells stained with AAA19750 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL11B Antibody (AAA19750, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of BCL11B using anti-BCL11B antibody (AAA19750) and anti-Tubulin Alpha antibody (M03989-3).BCL11B was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCL11B Antibody (AAA19750) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL11B antigen affinity purified polyclonal antibody (#AAA19750) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCL11B at approximately 116 kDa. The expected band size for BCL11B is at 96 kDa.)

BCL11B, Polyclonal Antibody (Cat# AAA19750)

Full Name

Anti-BCL11B Antibody Picoband

Gene Names

BCL11B; ATL1; RIT1; CTIP2; IMD49; CTIP-2; ZNF856B; ATL1-beta; ATL1-alpha; ATL1-delta; ATL1-gamma; hRIT1-alpha

Reactivity

Human, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

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IHC (Immunohistchemistry) (PLA2G2D Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue.)

PLA2G2D, Polyclonal Antibody (Cat# AAA21324)

Full Name

PLA2G2D Rabbit anti-Human Polyclonal Antibody

Reactivity

Mouse, Rat, Human

Applications

IF, IHC, IHC-P, WB

Purity

Affinity purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer using AAA26967 at dilution of 1:100)

SPDEF, Polyclonal Antibody (Cat# AAA26967)

Full Name

SPDEF Antibody

Gene Names

SPDEF; PDEF; bA375E1.3

Reactivity

Human, Mouse

Applications

EIA, WB, IHC

Purity

>95%, Protein G purified

Pricing

dnaK, Polyclonal Antibody (Cat# AAA26988)

Full Name

dnaK Antibody

Reactivity

Escherichia coli

Applications

EIA, WB

Purity

> 95%, Protein G purified

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of NOS2A Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

NOS2A, Polyclonal Antibody (Cat# AAA28780)

Full Name

NOS2A Antibody (Center)

Gene Names

NOS2; NOS; INOS; NOS2A; HEP-NOS

Reactivity

Human

Applications

WB, EIA, IHC, FC/FACS, IF

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

11beta-HSD1, Polyclonal Antibody (Cat# AAA29298)

Full Name

11beta-HSD1 Polyclonal Antibody

Gene Names

HSD11B1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

N4BP1, Polyclonal Antibody (Cat# AAA29078)

Full Name

N4BP1 Polyclonal Antibody

Reactivity

Human

Applications

WB, IHC-P

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using NDUFA4 Polyclonal Antibody.)

NDUFA4, Polyclonal Antibody (Cat# AAA30632)

Full Name

NDUFA4 Rabbit Polyclonal Antibody

Gene Names

NDUFA4; MLRQ; CI-9k; CI-MLRQ

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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NFE2L1, Polyclonal Antibody (Cat# AAA30570)

Full Name

NFE2L1 Polyclonal Antibody

Gene Names

NFE2L1; NRF1; TCF11; LCR-F1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)

KU70, Polyclonal Antibody (Cat# AAA30585)

Full Name

KU70 Rabbit Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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H2A.Z, Polyclonal Antibody (Cat# AAA31357)

Full Name

Acetyl-H2A.Z (Lys4/7/11/13) Antibody

Gene Names

H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (91%)

Applications

WB, IHC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of GLO1 using anti-GLO1 antibody (AAA19155).GLO1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GLO1 Antibody (AAA19155) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GLO1/Glyoxalase I, Polyclonal Antibody (Cat# AAA19155)

Full Name

Anti-GLO1/Glyoxalase I Picoband antibody

Gene Names

GLO1; GLYI; GLOD1; HEL-S-74

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

USP28, Polyclonal Antibody (Cat# AAA31438)

Full Name

Phospho-USP28 (Ser714) Antibody

Reactivity

Human, Mouse, Monkey

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (WB Suggested Anti-SLC33A1 antibody Titration: 1 ug/mLSample Type: Human liver)

SLC33A1, Polyclonal Antibody (Cat# AAA23512)

Full Name

SLC33A1 antibody - middle region

Gene Names

SLC33A1; AT1; AT-1; ACATN; SPG42; CCHLND

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-LRRC59 antibody (AAA19935).Overlay histogram showing JK cells stained with AAA19935 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRC59 Antibody (AAA19935, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of LRRC59 using anti-LRRC59 antibody (AAA19935).LRRC59 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat RH-35 whole cell lysates,Lane 3: mouse liver tissue lysates,Lane 4: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (#AAA19935) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human Caco-2 whole cell lysates,Lane 6: human A431 whole cell lysates,Lane 7: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (#AAA19935) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRC59 at approximately 35 kDa. The expected band size for LRRC59 is at 35 kDa.)

LRRC59, Polyclonal Antibody (Cat# AAA19935)

Full Name

Anti-LRRC59 Antibody Picoband

Gene Names

LRRC59; p34; PRO1855

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-H2AFY2/MACROH2A2 antibody (AAA19936).Overlay histogram showing PC-3 cells stained with AAA19936 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-H2AFY2/MACROH2A2 Antibody (AAA19936, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (AAA19936) and anti-Beta Tubulin antibody (M01857-3).H2AFY2/MACROH2A2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY2/MACROH2A2 Antibody (AAA19936) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY2/MACROH2A2 Antibody (AAA19936) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY2/MACROH2A2 Antibody (AAA19936) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY2/MACROH2A2 antigen affinity purified polyclonal antibody (#AAA19936) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for H2AFY2/MACROH2A2 at approximately 40 kDa. The expected band size for H2AFY2/MACROH2A2 is at 40 kDa.)

H2AFY2/MACROH2A2, Polyclonal Antibody (Cat# AAA19936)

Full Name

Anti-H2AFY2/MACROH2A2 Antibody Picoband

Gene Names

H2AFY2; macroH2A2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SRP19 antibody at dilution of 1:100 (40x lens).)

SRP19, Polyclonal Antibody (Cat# AAA28153)

Full Name

SRP19 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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P4HB, Polyclonal Antibody (Cat# AAA19258)

Full Name

Anti-P4HB Antibody

Gene Names

P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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ICC (Immunocytochemistry) (ICC staining HSP70 in SKBR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

HSP70, Polyclonal Antibody (Cat# AAA29967)

Full Name

HSP70 Antibody

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC

Purity

Peptide affinity purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

GGT1, Polyclonal Antibody (Cat# AAA29201)

Full Name

GGT1 Polyclonal Antibody

Gene Names

GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732

Reactivity

Human

Applications

WB, IHC-P

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-RARS/RARS1 antibody (AAA19748).Overlay histogram showing MCF-7 cells stained with AAA19748 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RARS/RARS1 Antibody (AAA19748, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of RARS/RARS1 using anti-RARS/RARS1 antibody (AAA19748) and anti-Tubulin Alpha antibody (M03989-3).RARS/RARS1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RARS/RARS1 Antibody (AAA19748) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS/RARS1 antigen affinity purified polyclonal antibody (#AAA19748) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RARS/RARS1 at approximately 70 kDa. The expected band size for RARS/RARS1 is at 75 kDa.)

RARS/RARS1, Polyclonal Antibody (Cat# AAA19748)

Full Name

Anti-RARS/RARS1 Antibody Picoband

Gene Names

RARS; HLD9; ArgRS; DALRD1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)

AP-1, Polyclonal Antibody (Cat# AAA28967)

Full Name

AP-1 Polyclonal Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF, IP

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TOM20 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

TOM20, Polyclonal Antibody (Cat# AAA22327)

Full Name

(KD Validated) TOM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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PFKFB3, Polyclonal Antibody (Cat# AAA31288)

Full Name

Phospho-PFKFB3 (Ser461) Antibody

Gene Names

PFKFB3; PFK2; IPFK2

Reactivity

Human, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IL-6Ralpha, Polyclonal Antibody (Cat# AAA29294)

Full Name

IL-6Ralpha Polyclonal Antibody

Gene Names

IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1

Reactivity

Human

Applications

WB, IHC-P

Pricing

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IF (Immunofluorescence) (Immunofluorescence staining of KLH-SMCCA1240+KLH-SMCCA2123 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1240+KLH-SMCCA2123 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

HSP60, Polyclonal Antibody (Cat# AAA27770)

Full Name

Anti-HSP60 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

WB, EIA, ICC, IF

Purity

Protein A & Antigen Affinity

Pricing

LINE-1, Polyclonal Antibody (Cat# AAA14722)

Full Name

LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)

Gene Names

L1TD1; ECAT11; RP5-1155K23.3

Reactivity

Human

Applications

EL/EIA, WB

Purity

Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Lamin B1, Polyclonal Antibody (Cat# AAA29060)

Full Name

Lamin B1 Polyclonal Antibody

Gene Names

LMNB1; LMN; ADLD; LMN2; LMNB

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

CYCS, Polyclonal Antibody (Cat# AAA29344)

Full Name

CYCS Polyclonal Antibody

Gene Names

CYCS; CYC; HCS; THC4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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LMNA, Polyclonal Antibody (Cat# AAA10641)

Full Name

LMNA Polyclonal Antibody

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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WB (Western Blot) (WB Suggested Anti-KCNK9 Antibody Titration: 1 ug/mlPositive Control: HepG2 cell lysate)

KCNK9, Polyclonal Antibody (Cat# AAA23441)

Full Name

KCNK9 antibody - N-terminal region

Gene Names

KCNK9; KT3.2; TASK3; K2p9.1; TASK-3

Reactivity

Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Dog

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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Lamin A/C, Polyclonal Antibody (Cat# AAA31378)

Full Name

Lamin A/C Antibody

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA, FC/FACS/FCM

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-NUB1 antibody (AAA19859).Overlay histogram showing K562 cells stained with AAA19859 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUB1 Antibody (AAA19859, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NUB1 using anti-NUB1 antibody (AAA19859) and anti-Beta Tubulin antibody (M01857-3).NUB1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUB1 Antibody (AAA19859) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUB1 Antibody (AAA19859) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUB1 Antibody (AAA19859) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Colo320 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human Raji whole cell lysates,Lane 6: monkey COS-7 whole cell lysates,Lane 8: human MCF-7 whole cell lysates,Lane 9: human A549 whole cell lysates,Lane 10: rat liver tissue lysates,Lane 11: rat PC-12 whole cell lysates,Lane 12: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUB1 antigen affinity purified polyclonal antibody (#AAA19859) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUB1 at approximately 75 kDa. The expected band size for NUB1 is at 71 kDa.)

NUB1, Polyclonal Antibody (Cat# AAA19859)

Full Name

Anti-NUB1 Antibody Picoband

Gene Names

NUB1; BS4; NUB1L; NYREN18

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HL-60 cells using anti-RNF34 antibody (A07903-1).Overlay histogram showing HL-60 cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF34 Antibody (A07903-1, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RNF34 and Tubulin beta using anti-RNF34 antibody (A07903-1) and anti-Tubulin beta antibody (M05613-4).RNF34 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (#AAA19899) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RNF34 at approximately 42 kDa. The expected band size for RNF34 is at 42 kDa.)

RNF34, Polyclonal Antibody (Cat# AAA19899)

Full Name

Anti-RNF34 Antibody Picoband

Gene Names

RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Spindlin-1, Polyclonal Antibody (Cat# AAA29123)

Full Name

Spindlin-1 Polyclonal Antibody

Gene Names

SPIN1; SPIN

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

UCH-L1, Polyclonal Antibody (Cat# AAA29154)

Full Name

UCH-L1 Polyclonal Antibody

Gene Names

UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of A-549 cells usingug PALLD Polyclonal Antibody.Western blot was performed from the immunoprecipitate using PALLD Polyclonal Antibody at a dilution of 1:1000.)

PALLD, Polyclonal Antibody (Cat# AAA22252)

Full Name

PALLD Polyclonal Antibody

Gene Names

PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TOLLIP antibody. Blue: DAPI for nuclear staining.)

TOLLIP, Polyclonal Antibody (Cat# AAA10741)

Full Name

TOLLIP Polyclonal Antibody

Gene Names

TOLLIP; IL-1RAcPIP

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HL-60 cells using anti-RPL32 antibody (AAA19570).Overlay histogram showing HL-60 cells stained with AAA19570 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (AAA19570, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RPL32, Polyclonal Antibody (Cat# AAA19570)

Full Name

Anti-RPL32 Antibody Picoband

Gene Names

RPL32; L32; PP9932

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

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Mucin 5 Subtype AC, Polyclonal Antibody (Cat# AAA20932)

Full Name

Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

Gene Names

Muc5ac; MGM; 2210005L13Rik

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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Application Data (Detection of Mouse anti Bovine CD86 in a flow cytometric analysis of bovine peripheral blood lymphocytes using Goat anti Mouse IgG:FITC (AAA12304))

IgG, Polyclonal Antibody (Cat# AAA12304)

Full Name

GOAT ANTI MOUSE IgG:FITC (RAT ADSORBED)

Applications

FC/FACS

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

BAF60a, Polyclonal Antibody (Cat# AAA28224)

Full Name

BAF60a Polyclonal Antibody

Gene Names

Smarcd1; Baf60a; D15Kz1; AA407987

Reactivity

Human, Mouse, Rat

Applications

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using CK-18 Polyclonal Antibody at dilution of 1:300)

CK-18, Polyclonal Antibody (Cat# AAA22342)

Full Name

CK-18 Polyclonal Antibody

Gene Names

Krt18; K18; CK18; Krt1-18

Reactivity

Human, Mouse

Applications

WB, IHC

Purity

Antigen Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of Raji cells using anti-PSMD4 antibody (AAA19792).Overlay histogram showing Raji cells stained with AAA19792 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD4 Antibody (AAA19792, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PSMD4 using anti-PSMD4 antibody (AAA19792) and anti-Beta Tubulin antibody (M01857-3).PSMD4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (#AAA19792) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.)

PSMD4, Polyclonal Antibody (Cat# AAA19792)

Full Name

Anti-PSMD4 Antibody Picoband

Gene Names

PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

EOMES, Polyclonal Antibody (Cat# AAA26888)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

IHC, WB

Purity

Purified by affinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

JAK1, Polyclonal Antibody (Cat# AAA28931)

Full Name

JAK1 (phospho Tyr1022) Polyclonal Antibody

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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hRIP3, Polyclonal Antibody (Cat# AAA11031)

Full Name

hRIP3 Antibody

Gene Names

RIPK3; RIP3

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

hRIP3 Antibody is Protein A purified.

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Smad3, Polyclonal Antibody (Cat# AAA28949)

Full Name

Smad3 (phospho Ser425) Polyclonal Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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<< Previous Next >>

WB (Western Blot) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Caveolin-1, Polyclonal Antibody (Cat# AAA28981)

Full Name

Caveolin-1 Polyclonal Antibody

Gene Names

CAV1; CGL3; BSCL3; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RTCB antibody (AAA19833).Overlay histogram showing Hela cells stained with AAA19833 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCB Antibody (AAA19833, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RTCB using anti-RTCB antibody (AAA19833).RTCB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (#AAA19833) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.)

RTCB, Polyclonal Antibody (Cat# AAA19833)

Full Name

Anti-RTCB Antibody Picoband

Gene Names

RTCB; FAAP; HSPC117; C22orf28; DJ149A16.6

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 1650-1700 of 3176 product results

BCL11B, Polyclonal Antibody (Cat# AAA19750)

PLA2G2D, Polyclonal Antibody (Cat# AAA21324)

SPDEF, Polyclonal Antibody (Cat# AAA26967)

dnaK, Polyclonal Antibody (Cat# AAA26988)

NOS2A, Polyclonal Antibody (Cat# AAA28780)

11beta-HSD1, Polyclonal Antibody (Cat# AAA29298)

N4BP1, Polyclonal Antibody (Cat# AAA29078)

NDUFA4, Polyclonal Antibody (Cat# AAA30632)

NFE2L1, Polyclonal Antibody (Cat# AAA30570)

KU70, Polyclonal Antibody (Cat# AAA30585)

H2A.Z, Polyclonal Antibody (Cat# AAA31357)

GLO1/Glyoxalase I, Polyclonal Antibody (Cat# AAA19155)

USP28, Polyclonal Antibody (Cat# AAA31438)

SLC33A1, Polyclonal Antibody (Cat# AAA23512)

LRRC59, Polyclonal Antibody (Cat# AAA19935)

H2AFY2/MACROH2A2, Polyclonal Antibody (Cat# AAA19936)

SRP19, Polyclonal Antibody (Cat# AAA28153)

P4HB, Polyclonal Antibody (Cat# AAA19258)

HSP70, Polyclonal Antibody (Cat# AAA29967)

GGT1, Polyclonal Antibody (Cat# AAA29201)

RARS/RARS1, Polyclonal Antibody (Cat# AAA19748)

AP-1, Polyclonal Antibody (Cat# AAA28967)

TOM20, Polyclonal Antibody (Cat# AAA22327)

PFKFB3, Polyclonal Antibody (Cat# AAA31288)

IL-6Ralpha, Polyclonal Antibody (Cat# AAA29294)

HSP60, Polyclonal Antibody (Cat# AAA27770)

LINE-1, Polyclonal Antibody (Cat# AAA14722)

Lamin B1, Polyclonal Antibody (Cat# AAA29060)

CYCS, Polyclonal Antibody (Cat# AAA29344)

LMNA, Polyclonal Antibody (Cat# AAA10641)

KCNK9, Polyclonal Antibody (Cat# AAA23441)

Lamin A/C, Polyclonal Antibody (Cat# AAA31378)

NUB1, Polyclonal Antibody (Cat# AAA19859)

RNF34, Polyclonal Antibody (Cat# AAA19899)

Spindlin-1, Polyclonal Antibody (Cat# AAA29123)

UCH-L1, Polyclonal Antibody (Cat# AAA29154)

PALLD, Polyclonal Antibody (Cat# AAA22252)

TOLLIP, Polyclonal Antibody (Cat# AAA10741)

RPL32, Polyclonal Antibody (Cat# AAA19570)

Mucin 5 Subtype AC, Polyclonal Antibody (Cat# AAA20932)

IgG, Polyclonal Antibody (Cat# AAA12304)

BAF60a, Polyclonal Antibody (Cat# AAA28224)

CK-18, Polyclonal Antibody (Cat# AAA22342)

PSMD4, Polyclonal Antibody (Cat# AAA19792)

EOMES, Polyclonal Antibody (Cat# AAA26888)

JAK1, Polyclonal Antibody (Cat# AAA28931)

hRIP3, Polyclonal Antibody (Cat# AAA11031)

Smad3, Polyclonal Antibody (Cat# AAA28949)

Caveolin-1, Polyclonal Antibody (Cat# AAA28981)

RTCB, Polyclonal Antibody (Cat# AAA19833)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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