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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1650-1700 of 10000 product results
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using PRKCB Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)
PRKCB, Polyclonal Antibody (Cat# AAA30564)
Full Name
PRKCB Polyclonal Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
EEF1A2, Polyclonal Antibody (Cat# AAA31289)
Full Name
Phospho-EEF1A2 (Ser358) Antibody
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using MAP2K3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
MAP2K3, Polyclonal Antibody (Cat# AAA22220)
Full Name
MAP2K3 Polyclonal Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
Transfection Control
(Anti-TPRA1 / GPR175 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)
TPRA1 / GPR175, Polyclonal Antibody (Cat# AAA12365)
Full Name
Rabbit Polyclonal to Human TPRA1 / GPR175
Gene Names
TPRA1; GPR175; TPRA40; TMEM227
Reactivity
Gibbon, Gorilla, Human, Monkey
Predicted Reactivity: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Predicted Reactivity: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using CHAF1B antibody. Blue: DAPI for nuclear staining.)
CHAF1B, Polyclonal Antibody (Cat# AAA28173)
Full Name
CHAF1B Polyclonal Antibody
Gene Names
CHAF1B; CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using ANXA8L2 antibody. Blue: DAPI for nuclear staining.)
ANXA8L1, Polyclonal Antibody (Cat# AAA28204)
Full Name
ANXA8L1 Polyclonal Antibody
Gene Names
ANXA8; ANX8; CH17-360D5.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.)
GFP11-Tag, Polyclonal Antibody (Cat# AAA27942)
Full Name
GFP11-tag Antibody
Applications
Western Blot
Purity
Peptide affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 using TOM20 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
TOM20, Polyclonal Antibody (Cat# AAA22204)
Full Name
TOM20 Polyclonal Antibody
Gene Names
TOMM20; MAS20; MOM19; TOM20
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
SAE2/UBA2, Polyclonal Antibody (Cat# AAA19171)
Full Name
Anti-SAE2/UBA2 Picoband antibody
Gene Names
UBA2; ARX; SAE2; HRIHFB2115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
WB (Western Blot)
(Western blot of Latexin (arrow) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2))
Latexin / MUM, Polyclonal Antibody (Cat# AAA12323)
Full Name
Rabbit Polyclonal to Human Latexin / MUM
Gene Names
LXN; ECI; TCI
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Purity
Immunoaffinity Purified
Pricing
WB (Western Blot)
(Anti-SLC10A1 antibody, AAA11511, Western blottingAll lanes: Anti SLC10A1 (AAA11511) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 38-56KDObserved bind size: 49KD)
SLC10A1, Polyclonal Antibody (Cat# AAA11511)
Full Name
Anti-SLC10A1 antibody
Gene Names
SLC10A1; NTCP
Reactivity
Human, Rat
Predict: Mouse
Predict: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 15. Flow Cytometry analysis of Raji cells using anti-Sam68/KHDRBS1 antibody (AAA19757).Overlay histogram showing Raji cells stained with AAA19757 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sam68/KHDRBS1 Antibody (AAA19757, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sam68/KHDRBS1, Polyclonal Antibody (Cat# AAA19757)
Full Name
Anti-Sam68/KHDRBS1 Antibody Picoband
Gene Names
KHDRBS1; p62; p68; Sam68
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using FXN/Frataxin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
FXN/Frataxin, Polyclonal Antibody (Cat# AAA22317)
Full Name
FXN/Frataxin Polyclonal Antibody
Gene Names
FXN; FA; X25; CyaY; FARR; FRDA
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-SOD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Placenta)
SOD2, Polyclonal Antibody (Cat# AAA23583)
Full Name
SOD2 antibody - N-terminal region
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
CD13, Polyclonal Antibody (Cat# AAA29304)
Full Name
CD13 Polyclonal Antibody
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.)
Bid, Polyclonal Antibody (Cat# AAA28769)
Full Name
Bid Antibody (BH3 Domain Specific)
Gene Names
BID; FP497
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Spindlin-1, Polyclonal Antibody (Cat# AAA29123)
Full Name
Spindlin-1 Polyclonal Antibody
Gene Names
SPIN1; SPIN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TOPBP1, Polyclonal Antibody (Cat# AAA31467)
Full Name
Phospho-TOPBP1 (Ser1159) Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%), Xenopus (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with OLR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
OLR1, Polyclonal Antibody (Cat# AAA28737)
Full Name
OLR1 Antibody (Center)
Gene Names
OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using USP5 antibody.)
USP5, Polyclonal Antibody (Cat# AAA30671)
Full Name
USP5 Rabbit Polyclonal Antibody
Gene Names
USP5; ISOT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse liver using KHDRBS1 at dilution of 1:100 (40x lens).)
KHDRBS1, Polyclonal Antibody (Cat# AAA30679)
Full Name
KHDRBS1 Rabbit Polyclonal Antibody
Gene Names
KHDRBS1; p62; p68; Sam68
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
eIF4B, Polyclonal Antibody (Cat# AAA31302)
Full Name
Phospho-eIF4B (Ser504) Antibody
Gene Names
EIF4B; EIF-4B; PRO1843
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
EIF2S2, Polyclonal Antibody (Cat# AAA31456)
Full Name
Phospho-EIF2S2 (Ser2) Antibody
Gene Names
EIF2S2; EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HEPA1-6 cells using anti-PI-16/PI16 antibody (AAA19330).Overlay histogram showing HEPA1-6 cells stained with AAA19330 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI-16/PI16 Antibody (AAA19330, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PI-16/PI16, Polyclonal Antibody (Cat# AAA19330)
Full Name
Anti-PI-16/PI16 Antibody
Gene Names
PI16; PSPBP; CRISP9; MSMBBP
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of LIN28B Antibody (N-term) in HL60 cell line lysates (35 ug/lane). LIN28B (arrow) was detected using the purified Pab.)
LIN28B, Polyclonal Antibody (Cat# AAA12320)
Full Name
Rabbit Polyclonal to Human LIN28B
Gene Names
LIN28B; CSDD2
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Ammonium sulfate precipitation
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using S100A10 antibody at dilution of 1:100 (40x lens).)
S100A10, Polyclonal Antibody (Cat# AAA10765)
Full Name
S100A10 Polyclonal Antibody
Gene Names
S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-PSMA2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
PSMA2, Polyclonal Antibody (Cat# AAA23568)
Full Name
PSMA2 antibody - N-terminal region
Gene Names
PSMA2; MU; HC3; PSC2; PMSA2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD79b, Polyclonal Antibody (Cat# AAA29197)
Full Name
CD79b Polyclonal Antibody
Gene Names
CD79B; B29; IGB; AGM6
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Raw264.7 cells using CD44 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
CD44, Polyclonal Antibody (Cat# AAA28336)
Full Name
CD44 Rabbit pAb
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat heart using IRS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IRS1, Polyclonal Antibody (Cat# AAA28342)
Full Name
IRS1 Rabbit pAb
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using PABPC1 at dilution of 1:100 (40x lens).)
PC1, Polyclonal Antibody (Cat# AAA30627)
Full Name
Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody
Gene Names
PABPC1; PAB1; PABP; PABP1; PABPC2; PABPL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)
COL5A2, Polyclonal Antibody (Cat# AAA29288)
Full Name
COL5A2 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
p27 Kip1, Polyclonal Antibody (Cat# AAA31387)
Full Name
Phospho-p27 Kip1 (Thr157) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Predicted Reactivity: Bovine (89%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (89%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody (AAA11516).Overlay histogram showing Hela cells stained with AAA11516 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (AAA11516,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Aquaporin 3, Polyclonal Antibody (Cat# AAA11516)
Full Name
Anti-Aquaporin 3 antibody
Gene Names
AQP3; GIL; AQP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-OXSM antibody (AAA19976).Overlay histogram showing MCF-7 cells stained with AAA19976 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSM Antibody (AAA19976, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
OXSM, Polyclonal Antibody (Cat# AAA19976)
Full Name
Anti-OXSM Antibody Picoband
Gene Names
OXSM; KS; KASI; FASN2D
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of SGK1 using anti- SGK1 antibody (AAA19225).SGK1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti- SGK1 Antibody (AAA19225) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SGK1, Polyclonal Antibody (Cat# AAA19225)
Full Name
Anti-SGK1 Antibody
Gene Names
SGK1; SGK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa using VDR Polyclonal Antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.)
VDR, Polyclonal Antibody (Cat# AAA22294)
Full Name
VDR Polyclonal Antibody
Gene Names
VDR; NR1I1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of Hela cells using anti-RPRD1B antibody (AAA19896).Overlay histogram showing Hela cells stained with AAA19896 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPRD1B Antibody (AAA19896, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPRD1B, Polyclonal Antibody (Cat# AAA19896)
Full Name
Anti-RPRD1B Antibody Picoband
Gene Names
RPRD1B; CREPT; NET60; C20orf77; dJ1057B20.2
Reactivity
Human, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
MYH-pan, Polyclonal Antibody (Cat# AAA29253)
Full Name
MYH-pan Polyclonal Antibody
Gene Names
MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody at dilution of 1:100 (40x lens).)
BRCA1, Polyclonal Antibody (Cat# AAA28245)
Full Name
BRCA1 Polyclonal Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody. Western blot was performed from the immunoprecipitate using EIF3H antibody at a dilition of 1:1000.)
EIF3H, Polyclonal Antibody (Cat# AAA28265)
Full Name
EIF3H Polyclonal Antibody
Gene Names
EIF3H; EIF3S3; eIF3-p40; eIF3-gamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 600ug extracts of Mouse skeletal muscle cells using 3ug COL1A1 antibody . Western blot was performed from the immunoprecipitate using COL1A1 antibody at a dilition of 1:1000.)
COL1A1, Polyclonal Antibody (Cat# AAA28340)
Full Name
COL1A1 Rabbit pAb
Gene Names
COL1A1; OI4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)
PGD, Polyclonal Antibody (Cat# AAA29097)
Full Name
PGD Polyclonal Antibody
Gene Names
PGD; 6PGD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using STAT1 at dilution of 1:100 (40x lens).)
STAT1, Polyclonal Antibody (Cat# AAA30601)
Full Name
STAT1 Rabbit Polyclonal Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(FOLR2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
FOLR2, Polyclonal Antibody (Cat# AAA28783)
Full Name
FOLR2 Antibody (N-term)
Gene Names
FOLR2; FBP; FR-P3; FR-BETA; BETA-HFR; FBP/PL-1
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
BRCA1, Polyclonal Antibody (Cat# AAA28973)
Full Name
BRCA1 Polyclonal Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using DES at dilution of 1:100 (40x lens).)
DES, Polyclonal Antibody (Cat# AAA30590)
Full Name
DES Rabbit Polyclonal Antibody
Gene Names
DES; CSM1; CSM2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody.)
ZNRF3, Polyclonal Antibody (Cat# AAA29818)
Full Name
ZNRF3 Polyclonal Antibody
Gene Names
ZNRF3; RNF203; BK747E2.3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
JIP1, Polyclonal Antibody (Cat# AAA31318)
Full Name
Phospho-JIP1 (Thr103) Antibody
Gene Names
MAPK8IP1; IB1; JIP1; JIP-1; PRKM8IP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.