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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Thioredoxin, Polyclonal Antibody (Cat# AAA29875)
Full Name
Thioredoxin Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Purity
Peptide affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using TXNRD1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
TXNRD1, Polyclonal Antibody (Cat# AAA30643)
Full Name
TXNRD1 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of RT4 cells using anti-RFX3 antibody (AAA19892).Overlay histogram showing RT4 cells stained with AAA19892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFX3 Antibody (AAA19892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RFX3, Polyclonal Antibody (Cat# AAA19892)
Full Name
Anti-RFX3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
EIF4EBP1, Polyclonal Antibody (Cat# AAA22196)
Full Name
EIF4EBP1 Polyclonal Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
WB (Western Blot)
(Positive WB detected in: recombinant proteinAll lanes: MELT Antibody at 1:1000Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 17 kDaObserved band size: 17 kDa)
MELT, Polyclonal Antibody (Cat# AAA27057)
Full Name
Rabbit anti-Apis mellifera (Honeybee) MELT Polyclonal Antibody
Gene Names
Melt; GB10355
Reactivity
Apis mellifera
Applications
ELISA (EIA), Western Blot (WB)
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Raf-1, Polyclonal Antibody (Cat# AAA29105)
Full Name
Raf-1 Polyclonal Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
CD158k, Polyclonal Antibody (Cat# AAA29361)
Full Name
CD158k Polyclonal Antibody
Gene Names
KIR3DL2; 3DL2; p140; NKAT4; CD158K; NKAT-4; NKAT4B; KIR-3DL2
Reactivity
Human
Applications
Immunohistochemistry-Paraffin (IHC-P)
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
PKC delta, Polyclonal Antibody (Cat# AAA31409)
Full Name
Phospho-PKC delta (Ser664) Antibody
Gene Names
PRKCD; MAY1; PKCD; nPKC-delta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using CDK6 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
CDK6, Polyclonal Antibody (Cat# AAA22194)
Full Name
CDK6 Polyclonal Antibody
Gene Names
CDK6; PLSTIRE
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using WNT10B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
WNT10B, Polyclonal Antibody (Cat# AAA28334)
Full Name
WNT10B Rabbit pAb
Gene Names
WNT10B; SHFM6; WNT-12
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
HSP 90, Polyclonal Antibody (Cat# AAA28846)
Full Name
HSP 90 (Acetyl Lys292/284) Polyclonal Antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DDX6 at 1:1000 dilution.)
DDX6, Polyclonal Antibody (Cat# AAA30638)
Full Name
DDX6 Rabbit Polyclonal Antibody
Gene Names
DDX6; P54; RCK; HLR2
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-NFAT3 (Ser289) in lysates of HepG2 Ca2+ 40uM 30', using Phospho-NFAT3 (Ser289) Antibody.)
NFAT3, Polyclonal Antibody (Cat# AAA31157)
Full Name
Phospho-NFAT3 (Ser289) Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of PRDM1/Blimp1 using anti-PRDM1/Blimp1 antibody (AAA19133).PRDM1/Blimp1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRDM1/Blimp1 Antibody (AAA19133) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PRDM1/Blimp1, Polyclonal Antibody (Cat# AAA19133)
Full Name
Anti-PRDM1/Blimp1 Picoband antibody
Gene Names
PRDM1; BLIMP1; PRDI-BF1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using BCAP29 antibody at dilution of 1:100 (40x lens).)
BCAP29, Polyclonal Antibody (Cat# AAA28093)
Full Name
BCAP29 Polyclonal Antibody
Gene Names
BCAP29; BAP29
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC)
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Smad2, Polyclonal Antibody (Cat# AAA29117)
Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
Thyroglobulin, Polyclonal Antibody (Cat# AAA29373)
Full Name
Thyroglobulin Polyclonal Antibody
Gene Names
TG; TGN; AITD3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin (IHC-P)
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
BAG3, Polyclonal Antibody (Cat# AAA31421)
Full Name
Phospho-BAG3 (Tyr457) Antibody
Gene Names
BAG3; BIS; BAG-3; CAIR-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of 293T cells using anti-NCOA5 antibody (AAA19894).Overlay histogram showing 293T cells stained with AAA19894 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA5 Antibody (AAA19894, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
NCOA5, Polyclonal Antibody (Cat# AAA19894)
Full Name
Anti-NCOA5 Antibody Picoband
Gene Names
NCOA5; CIA; bA465L10.6
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: omp38 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52.5 kDaObserved band size: 66 kDa.)
omp38, Polyclonal Antibody (Cat# AAA27062)
Full Name
Rabbit anti-Acinetobacter baumannii (strain 17978/CIP 53.77/LMG 1025/NCDC KC755/5377) omp38 Polyclonal Antibody
Reactivity
Acinetobacter baumannii
Applications
ELISA (EIA), Western Blot (WB)
Purity
Protein G
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat heart using IRS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IRS1, Polyclonal Antibody (Cat# AAA28342)
Full Name
IRS1 Rabbit pAb
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
IL-1F7, Polyclonal Antibody (Cat# AAA29366)
Full Name
IL-1F7 Polyclonal Antibody
Gene Names
IL37; FIL1; FIL1Z; IL-1H; IL-37; IL1F7; IL1H4; IL-1F7; IL-1H4; IL1RP1; IL-1RP1; FIL1(ZETA)
Reactivity
Human
Applications
Immunohistochemistry-Paraffin (IHC-P)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)
NADPH Oxidase 4, Polyclonal Antibody (Cat# AAA29878)
Full Name
NADPH Oxidase 4 Antibody
Gene Names
NOX4; KOX; KOX-1; RENOX
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Purity
Peptide affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Zyxin (Tyr316) in lysates of Jurkat paclitaxel 1uM 24h, using Phospho-Zyxin (Tyr316) Antibody.)
Zyxin, Polyclonal Antibody (Cat# AAA31158)
Full Name
Phospho-Zyxin (Tyr316) Antibody
Gene Names
ZYX; ESP-2; HED-2
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using SERPINA7 antibody at dilution of 1:100 (40x lens).)
SERPINA7, Polyclonal Antibody (Cat# AAA28088)
Full Name
SERPINA7
Gene Names
SERPINA7; TBG
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry (IHC)
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunocytochemistry: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Rock-1, Polyclonal Antibody (Cat# AAA29112)
Full Name
Rock-1 Polyclonal Antibody
Gene Names
ROCK1; ROCK-I; P160ROCK
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with eIF-4E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
eIF-4E, Polyclonal Antibody (Cat# AAA29880)
Full Name
eIF-4E Antibody
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Purity
Peptide affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-IGF2BP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysateIGF2BP2 is supported by BioGPS gene expression data to be expressed in HT1080)
IGF2BP2, Polyclonal Antibody (Cat# AAA23481)
Full Name
IGF2BP2 antibody - middle region
Gene Names
IGF2BP2; IMP2; IMP-2; VICKZ2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast
Applications
Western Blot (WB)
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using TOPBP1 antibody. Blue: DAPI for nuclear staining.)
TOPBP1, Polyclonal Antibody (Cat# AAA28089)
Full Name
TOPBP1 Polyclonal Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity Purification
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using YAP1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
YAP1, Polyclonal Antibody (Cat# AAA28345)
Full Name
YAP1 Rabbit pAb
Gene Names
YAP1; YAP; YKI; YAP2; YAP65
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
RXRgamma, Polyclonal Antibody (Cat# AAA29113)
Full Name
RXRgamma Polyclonal Antibody
Gene Names
RXRG; RXRC; NR2B3
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
LT-beta, Polyclonal Antibody (Cat# AAA29369)
Full Name
LT-beta Polyclonal Antibody
Gene Names
LTB; p33; TNFC; TNFSF3
Reactivity
Human
Applications
Immunohistochemistry-Paraffin (IHC-P)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Histone Deacetylase 2, Polyclonal Antibody (Cat# AAA29881)
Full Name
Histone Deacetylase 2 Antibody
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Purity
Protein affinity purified
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
FGFR1/2/3/4, Polyclonal Antibody (Cat# AAA31417)
Full Name
Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human kidney cancer using BSG Antibody at dilution of 1:200 (40x lens).)
BSG, Polyclonal Antibody (Cat# AAA10682)
Full Name
BSG Polyclonal Antibody
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry (IHC)
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Cdc20 using anti-Cdc20 antibody (AAA19132).Cdc20 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody (AAA19132) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cdc20/P55 Cdc, Polyclonal Antibody (Cat# AAA19132)
Full Name
Anti-Cdc20/P55 Cdc Picoband Antibody
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry (IHC), Western Blot (WB)
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 15. IHC analysis of SCAMP3 using anti-SCAMP3 antibody (AAA19900).SCAMP3 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAMP3 antigen affinity purified polyclonal antibody (#AAA19900) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCAMP3 at approximately 36 kDa. The expected band size for SCAMP3 is at 38 kDa.)
SCAMP3, Polyclonal Antibody (Cat# AAA19900)
Full Name
Anti-SCAMP3 Antibody Picoband
Gene Names
SCAMP3; C1orf3
Reactivity
Human, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Rabbit Anti-NONO Antibody Catalog Number: AAA23484 Paraffin Embedded Tissue: Human cardiac cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X)
NONO, Polyclonal Antibody (Cat# AAA23484)
Full Name
NONO antibody - C-terminal region
Gene Names
NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Reactivity
Tested Species Reactivity: Human (Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat)
Applications
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Purity
Protein A purified
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using SIRT1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
SIRT1, Polyclonal Antibody (Cat# AAA28348)
Full Name
[KO Validated] SIRT1 Rabbit pAb
Gene Names
SIRT1; SIR2L1
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Skp2 p45, Polyclonal Antibody (Cat# AAA29116)
Full Name
Skp2 p45 Polyclonal Antibody
Gene Names
SKP2; p45; FBL1; FLB1; FBXL1
Reactivity
Human
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with UBE1C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green).)
UBE1C, Polyclonal Antibody (Cat# AAA29884)
Full Name
UBE1C Antibody
Gene Names
UBA3; NAE2; UBE1C; hUBA3
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse kidney using COL1A1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
COL1A1, Polyclonal Antibody (Cat# AAA22203)
Full Name
COL1A1 Polyclonal Antibody
Gene Names
COL1A1; OI4
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
SIRT1, Polyclonal Antibody (Cat# AAA29115)
Full Name
SIRT1 Polyclonal Antibody
Gene Names
SIRT1; SIR2L1
Reactivity
Human, Mouse
Applications
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Pricing
ICC (Immunocytochemistry)
(ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Calpain 1, Polyclonal Antibody (Cat# AAA29883)
Full Name
Calpain 1 Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody at dilution of 1:100 (40x lens).)
HADH, Polyclonal Antibody (Cat# AAA10684)
Full Name
HADH Polyclonal Antibody
Gene Names
HADH; HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC)
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using SIAH1 antibody. Blue: DAPI for nuclear staining.)
SIAH1, Polyclonal Antibody (Cat# AAA10680)
Full Name
SIAH1 Polyclonal Antibody
Gene Names
SIAH1; SIAH1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using SIRT3 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
SIRT3, Polyclonal Antibody (Cat# AAA28346)
Full Name
SIRT3 Rabbit pAb
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
6CKine, Polyclonal Antibody (Cat# AAA29370)
Full Name
6CKine Polyclonal Antibody
Gene Names
CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
Reactivity
Human
Applications
Immunohistochemistry-Paraffin (IHC-P)
Pricing
WB (Western Blot)
(Western blot analysis of extracts of HCT116 cells, using Bcl-2 at 1:1000 dilution.HCT116 cells treated with different concentrations of IL-8.)
Bcl-2, Polyclonal Antibody (Cat# AAA30650)
Full Name
Bcl-2 Rabbit Polyclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CD22, Polyclonal Antibody (Cat# AAA31418)
Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Predicted Reactivity: Horse (83%)
Applications
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
