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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2700-2750 of 10000 product results
WB (Western Blot)
(WB Suggested Anti-KCNN4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate)
KCNN4, Polyclonal Antibody (Cat# AAA23440)
Full Name
KCNN4 antibody - C-terminal region
Gene Names
KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Reactivity
Cow, Horse, Human, Pig, Rat
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-HNRPA3 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHNRNPA3 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells)
HNRPA3, Polyclonal Antibody (Cat# AAA23489)
Full Name
HNRPA3 antibody - N-terminal region
Gene Names
HNRNPA3; FBRNP; HNRPA3; D10S102; 2610510D13Rik
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: ACTBSample Type: JurkatAntibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in Jurkat)
ACTB, Polyclonal Antibody (Cat# AAA23467)
Full Name
ACTB Antibody - middle region
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Cow, Goat, Horse, Human, Mouse, Pig, Rat, Sheep, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 15. IHC analysis of SCAMP3 using anti-SCAMP3 antibody (AAA19900).SCAMP3 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCAMP3 Antibody (AAA19900) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAMP3 antigen affinity purified polyclonal antibody (#AAA19900) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCAMP3 at approximately 36 kDa. The expected band size for SCAMP3 is at 38 kDa.)
SCAMP3, Polyclonal Antibody (Cat# AAA19900)
Full Name
Anti-SCAMP3 Antibody Picoband
Gene Names
SCAMP3; C1orf3
Reactivity
Human, Rat
Applications
EIA, IF, IHC, ICC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM antibody at dilution of 1:100 (40x lens).)
HNRNPM, Polyclonal Antibody (Cat# AAA28111)
Full Name
HNRNPM
Gene Names
HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Apelin, Polyclonal Antibody (Cat# AAA29162)
Full Name
Apelin Polyclonal Antibody
Gene Names
APLN; APEL; XNPEP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
ICC (Immunocytochemistry)
(ICC staining TROP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
TROP2, Polyclonal Antibody (Cat# AAA29936)
Full Name
TROP2 Antibody
Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Reactivity
Human
Applications
WB, ICC, IHC
Purity
Protein affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using NOTCH1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
NOTCH1, Polyclonal Antibody (Cat# AAA22197)
Full Name
NOTCH1 Polyclonal Antibody
Gene Names
NOTCH1; hN1; TAN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using CDK19 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
CDK19, Polyclonal Antibody (Cat# AAA22259)
Full Name
CDK19 Polyclonal Antibody
Gene Names
CDK19; CDK11; CDC2L6; bA346C16.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: FAM46CSample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)
CYP11A1, Polyclonal Antibody (Cat# AAA23543)
Full Name
CYP11A1 antibody - middle region
Gene Names
CYP11A1; CYP11A; CYPXIA1; P450SCC
Reactivity
Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
EZH2, Polyclonal Antibody (Cat# AAA28665)
Full Name
EZH2 Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with ERGI3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ERGI3, Polyclonal Antibody (Cat# AAA29942)
Full Name
ERGI3 Antibody
Gene Names
ERGIC3; Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of K562 cells using anti-EIF5 antibody (AAA19525).Overlay histogram showing K562 cells stained with AAA19525 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF5 Antibody (AAA19525, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EIF5, Polyclonal Antibody (Cat# AAA19525)
Full Name
Anti-EIF5 Antibody Picoband
Gene Names
EIF5; EIF-5; EIF-5A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 12. IF analysis of SAE1 using anti-SAE1 antibody (AAA19526).SAE1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-SAE1 Antibody (AAA19526) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SAE1, Polyclonal Antibody (Cat# AAA19526)
Full Name
Anti-SAE1 Antibody Picoband
Gene Names
SAE1; AOS1; SUA1; UBLE1A; HSPC140
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using MTHFD2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
MTHFD2, Polyclonal Antibody (Cat# AAA22174)
Full Name
MTHFD2 Polyclonal Antibody
Gene Names
MTHFD2; NMDMC
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-SSX4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
SSX4, Polyclonal Antibody (Cat# AAA23446)
Full Name
SSX4 antibody - middle region
Gene Names
SSX4; CT5.4
Reactivity
Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-HNRPA0 Antibody Titration: 0.625ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate)
HNRPA0, Polyclonal Antibody (Cat# AAA23482)
Full Name
HNRPA0 antibody - middle region
Gene Names
HNRNPA0; HNRPA0
Reactivity
Cow, Dog, Human, Pig
Applications
IF, IP, IHC, WB
Purity
Protein A purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (AAA19167).Cyclophilin B was detected in paraffin-embedded section of rat thyroid gland tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cyclophilin B Antibody (AAA19167) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cyclophilin B, Polyclonal Antibody (Cat# AAA19167)
Full Name
Anti-Cyclophilin B Picoband Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-CTRL Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)
CTRL, Polyclonal Antibody (Cat# AAA23429)
Full Name
CTRL antibody - N-terminal region
Gene Names
CTRL; CTRL1
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Dog, Pig
Predicted Species Reactivity: Human, Mouse, Rat, Dog, Pig
Applications
WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using ATXN2 antibody (AAA28401) at dilution of 1:100. Blue: DAPI for nuclear staining.)
ATXN2, Polyclonal Antibody (Cat# AAA28401)
Full Name
ATXN2 Rabbit pAb
Gene Names
ATXN2; ATX2; SCA2; ASL13; TNRC13
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Neuro-2a cells using anti-KMO antibody (AAA19550).Overlay histogram showing Neuro-2a cells stained with AAA19550 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KMO Antibody (AAA19550, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KMO, Polyclonal Antibody (Cat# AAA19550)
Full Name
Anti-KMO Antibody Picoband
Gene Names
KMO; dJ317G22.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of K562 cells using anti-RPL32 antibody (AAA19571).Overlay histogram showing K562 cells stained with AAA19571 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (AAA19571, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RPL32, Polyclonal Antibody (Cat# AAA19571)
Full Name
Anti-RPL32 Antibody Picoband
Gene Names
RPL32; L32; PP9932
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U87 cells using anti-TLS/FUS antibody (AAA19406).Overlay histogram showing U87 cells stained with AAA19406 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLS/FUS Antibody (AAA19406, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TLS/FUS, Polyclonal Antibody (Cat# AAA19406)
Full Name
Anti-TLS/FUS Antibody Picoband
Gene Names
FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 30ug/ml Rabbit Anti-Human IL35 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))
Interleukin 35 (IL35), Polyclonal Antibody (Cat# AAA20105)
Full Name
Polyclonal Antibody to Interleukin 35 (IL35)
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of HepG2 cells using anti-MYH14 antibody (AAA19818).Overlay histogram showing HepG2 cells stained with AAA19818 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYH14 Antibody (AAA19818, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MYH14, Polyclonal Antibody (Cat# AAA19818)
Full Name
Anti-MYH14 Antibody Picoband
Gene Names
MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U251 cells using anti-RPS5 antibody (AAA19817).Overlay histogram showing U251 cells stained with AAA19817 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS5 Antibody (AAA19817, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS5, Polyclonal Antibody (Cat# AAA19817)
Full Name
Anti-RPS5 Antibody Picoband
Gene Names
RPS5; S5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 7. IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (AAA19843).FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (AAA19843, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FOX2/RBM9/RBFOX2, Polyclonal Antibody (Cat# AAA19843)
Full Name
Anti-FOX2/RBM9/RBFOX2 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-BRD3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human brain)
BRD3, Polyclonal Antibody (Cat# AAA23432)
Full Name
BRD3 antibody - middle region
Gene Names
BRD3; ORFX; RING3L
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SSB Antibody Titration: 0.25 ug/mlPositive Control: Jurkat cell lysateSSB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells)
SSB, Polyclonal Antibody (Cat# AAA23478)
Full Name
SSB antibody - N-terminal region
Gene Names
SSB; La; LARP3; La/SSB
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U87 cells using anti-PSMB5/MB1 antibody (AAA19274).Overlay histogram showing U87 cells stained with AAA19274 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB5/MB1 Antibody (AAA19274, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PSMB5/MB1, Polyclonal Antibody (Cat# AAA19274)
Full Name
Anti-PSMB5/MB1 Antibody
Gene Names
PSMB5; X; MB1; LMPX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of RH35 cells using anti-TWIST1/2 antibody (AAA19412).Overlay histogram showing RH35 cells stained with AAA19412 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TWIST1/2 Antibody (AAA19412, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TWIST1/2, Polyclonal Antibody (Cat# AAA19412)
Full Name
Anti-TWIST1/2 Antibody Picoband
Gene Names
TWIST1; CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HeLa cells using anti-NDUFS2 antibody (AAA19555).Overlay histogram showing HeLa cells stained with AAA19555 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS2 Antibody (AAA19555, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFS2, Polyclonal Antibody (Cat# AAA19555)
Full Name
Anti-NDUFS2 Antibody Picoband
Gene Names
NDUFS2; CI-49
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of beta Catenin using anti-beta Catenin antibody (AAA19129).beta Catenin was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-beta Catenin Antibody (AAA19129) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
beta Catenin, Polyclonal Antibody (Cat# AAA19129)
Full Name
Anti-beta Catenin Picoband Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of FH using anti-FH antibody (AAA19157).FH was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FH Antibody (AAA19157) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FH/Fumarase, Polyclonal Antibody (Cat# AAA19157)
Full Name
Anti-FH/Fumarase Picoband Antibody
Gene Names
FH; MCL; FMRD; LRCC; HLRCC; MCUL1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Hip1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
HIP1, Polyclonal Antibody (Cat# AAA29933)
Full Name
HIP1 Antibody
Gene Names
HIP1; HIP-I; ILWEQ
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U937 cells using anti-SEC14L3/TAP2 antibody (AAA19342).Overlay histogram showing U937 cells stained with AAA19342 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC14L3/TAP2 Antibody (AAA19342, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SEC14L3/TAP2, Polyclonal Antibody (Cat# AAA19342)
Full Name
Anti-SEC14L3/TAP2 Antibody
Gene Names
SEC14L3; TAP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of JK cells using anti-FKBP135/FKBP15 antibody (AAA19620).Overlay histogram showing JK cells stained with AAA19620 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FKBP135/FKBP15 Antibody (AAA19620, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FKBP135/FKBP15, Polyclonal Antibody (Cat# AAA19620)
Full Name
Anti-FKBP135/FKBP15 Antibody Picoband
Gene Names
FKBP15; FKBP133; PPP1R76; KIAA0674
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(Dilution: WB 1:1000-2000, IHC 1:100-200)
KCNK9, Polyclonal Antibody (Cat# AAA28901)
Full Name
KCNK9 (TASK-3) Polyclonal Antibody
Gene Names
KCNK9; KT3.2; TASK3; K2p9.1; TASK-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U937 cells using anti-NDUFAF4 antibody (AAA19964).Overlay histogram showing U937 cells stained with AAA19964 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFAF4 Antibody (AAA19964, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFAF4, Polyclonal Antibody (Cat# AAA19964)
Full Name
Anti-NDUFAF4 Antibody Picoband
Gene Names
NDUFAF4; My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-MAT2A antibody (AAA19521).Overlay histogram showing MCF-7 cells stained with AAA19521 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAT2A Antibody (AAA19521, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAT2A, Polyclonal Antibody (Cat# AAA19521)
Full Name
Anti-MAT2A Antibody Picoband
Gene Names
MAT2A; MATA2; MATII; SAMS2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-KAT3B/P300/EP300 antibody (AAA19390).Overlay histogram showing 293T cells stained with AAA19390 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT3B/P300/EP300 Antibody (AAA19390, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
KAT3B/p300/EP300, Polyclonal Antibody (Cat# AAA19390)
Full Name
Anti-KAT3B/p300/EP300 Antibody Picoband
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of Daudi cells using anti-TOMM34 antibody (AAA19574).Overlay histogram showing Daudi cells stained with AAA19574 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM34 Antibody (AAA19574, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOMM34, Polyclonal Antibody (Cat# AAA19574)
Full Name
Anti-TOMM34 Antibody Picoband
Gene Names
TOMM34; TOM34; URCC3; HTOM34P
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 15. IHC analysis of NRTN using anti-NRTN antibody (AAA19855).NRTN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NRTN Antibody (AAA19855) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRTN antigen affinity purified polyclonal antibody (#AAA19855) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NRTN at approximately 22 kDa. The expected band size for NRTN is at 22 kDa.)
NRTN, Polyclonal Antibody (Cat# AAA19855)
Full Name
Anti-NRTN Antibody Picoband
Gene Names
NRTN; NTN
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of Raji cells using anti-PSMD14 antibody (AAA19874).Overlay histogram showing Raji cells stained with AAA19874 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD14 Antibody (AAA19874, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
POH1/PSMD14, Polyclonal Antibody (Cat# AAA19874)
Full Name
Anti-POH1/PSMD14 Antibody Picoband
Gene Names
PSMD14; PAD1; POH1; RPN11
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blotAll lanes: APOA1antibody at 2ug/mlLane 1:mouse liver tissueLane 2:mouse lung tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)
Apolipoprotein A-I, Polyclonal Antibody (Cat# AAA18816)
Full Name
Rabbit anti-Bovine Apolipoprotein A-I polyclonal Antibody
Reactivity
Bovine, Mouse
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using PTRF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PTRF, Polyclonal Antibody (Cat# AAA28370)
Full Name
PTRF Rabbit pAb
Gene Names
PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(TYSY Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
TYSY, Polyclonal Antibody (Cat# AAA28647)
Full Name
TYSY Antibody (C-term)
Gene Names
TYMS; TS; TMS; HST422
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
CACNG4, Polyclonal Antibody (Cat# AAA29944)
Full Name
CACNG4 Antibody
Reactivity
Human, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: CCDC109BSample Tissue: Mouse SP2/0 Whole Cell lysatesAntibody Dilution: 1ug/ml)
MCUB, Polyclonal Antibody (Cat# AAA23604)
Full Name
MCUB Antibody - middle region
Gene Names
Mcub; Ccdc109b; 9030408N13Rik
Reactivity
Tested Reactivity: Mouse
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Purity
Affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-Tubulin Alpha antibody (AAA19805).Overlay histogram showing 293T cells stained with AAA19805 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin Alpha Antibody (AAA19805, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Alpha Tubulin/TUBA1C, Polyclonal Antibody (Cat# AAA19805)
Full Name
Anti-Alpha Tubulin/TUBA1C Antibody Picoband
Gene Names
TUBA1C; TUBA6; bcm948
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.