Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2650-2700 of 10000 product results
WB (Western Blot)
(WB Suggested Anti-HOXB2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)
HOXB2, Polyclonal Antibody (Cat# AAA23444)
Full Name
HOXB2 antibody - N-terminal region
Gene Names
HOXB2; K8; HOX2; HOX2H; Hox-2.8
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MYLK, Polyclonal Antibody (Cat# AAA19154)
Full Name
Anti-MYLK Picoband antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
BubR1, Polyclonal Antibody (Cat# AAA29938)
Full Name
BubR1 Antibody
Gene Names
BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta
Reactivity
Human
Applications
WB, ICC, IHC, FC/FACS
Purity
Protein affinity purified
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Lamin B1, Polyclonal Antibody (Cat# AAA29168)
Full Name
Lamin B1 Polyclonal Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of CACO-2 cells using anti-GRX2/GLRX2 antibody (AAA19305).Overlay histogram showing CACO-2 cells stained with AAA19305 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRX2/GLRX2 Antibody (AAA19305, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GRX2/GLRX2, Polyclonal Antibody (Cat# AAA19305)
Full Name
Anti-GRX2/GLRX2 Antibody
Gene Names
GLRX2; GRX2; CGI-133
Reactivity
Human, Mouse
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (AAA19447).EAAT1/SLC1A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-EAAT1/SLC1A3 Antibody (AAA19447) overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
EAAT1/SLC1A3, Polyclonal Antibody (Cat# AAA19447)
Full Name
Anti-EAAT1/SLC1A3 Antibody Picoband
Gene Names
SLC1A3; EA6; EAAT1; GLAST; GLAST1
Reactivity
Mouse, Rat
Applications
IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Raji cells using anti-HIP1R antibody (AAA19533).Overlay histogram showing Raji cells stained with AAA19533 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIP1R Antibody (AAA19533, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HIP1R, Polyclonal Antibody (Cat# AAA19533)
Full Name
Anti-HIP1R Antibody Picoband
Gene Names
HIP1R; HIP3; HIP12; ILWEQ
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-CPT1A antibody (AAA19411).Overlay histogram showing U251 cells stained with AAA19411 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPT1A Antibody (AAA19411, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CPT1A, Polyclonal Antibody (Cat# AAA19411)
Full Name
Anti-CPT1A Antibody Picoband
Gene Names
CPT1A; CPT1; CPT1-L; L-CPT1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HepG2 cells using anti-RPN1 antibody (AAA19832).Overlay histogram showing HepG2 cells stained with AAA19832 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPN1 Antibody (AAA19832, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPN1, Polyclonal Antibody (Cat# AAA19832)
Full Name
Anti-RPN1 Antibody Picoband
Gene Names
RPN1; OST1; RBPH1
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of RT4 cells using anti-PDXK antibody (AAA19875).Overlay histogram showing RT4 cells stained with AAA19875 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDXK Antibody (AAA19875, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDXK, Polyclonal Antibody (Cat# AAA19875)
Full Name
Anti-PDXK Antibody Picoband
Gene Names
PDXK; PKH; PNK; PRED79; C21orf97; C21orf124
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-IGF2BP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HT1080 cell lysateIGF2BP2 is supported by BioGPS gene expression data to be expressed in HT1080)
IGF2BP2, Polyclonal Antibody (Cat# AAA23481)
Full Name
IGF2BP2 antibody - middle region
Gene Names
IGF2BP2; IMP2; IMP-2; VICKZ2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of IRAK4 expression in MCF7 (A), Jurkat (B), Raji (C) whole cell lysates.)
IRAK4, Polyclonal Antibody (Cat# AAA27860)
Full Name
Anti-IRAK4 Antibody
Gene Names
IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using ATP1B1 antibody at dilution of 1:100 (40x lens).)
ATP1B1, Polyclonal Antibody (Cat# AAA28138)
Full Name
ATP1B1 Polyclonal Antibody
Gene Names
ATP1B1; ATP1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of 293T cells using anti-XRCC4 antibody (AAA19407).Overlay histogram showing 293T cells stained with AAA19407 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRCC4 Antibody (AAA19407, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
XRCC4, Polyclonal Antibody (Cat# AAA19407)
Full Name
Anti-XRCC4 Antibody Picoband
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of RT4 cells using anti-BBS9 antibody (AAA19581).Overlay histogram showing RT4 cells stained with AAA19581 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BBS9 Antibody (AAA19581, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BBS9, Polyclonal Antibody (Cat# AAA19581)
Full Name
Anti-BBS9 Antibody Picoband
Gene Names
BBS9; B1; D1; C18; PTHB1
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-NDUFS8 antibody (AAA19597).Overlay histogram showing 293T cells stained with AAA19597 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (AAA19597, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFS8, Polyclonal Antibody (Cat# AAA19597)
Full Name
Anti-NDUFS8 Antibody Picoband
Gene Names
NDUFS8; TYKY; CI-23k; CI23KD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with AAA14796 at 1:25 dilution, followed by Dylight® 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA14796)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)
Reactivity
Human
Applications
EL/EIA, WB, IHC, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat fetal liver using Survivin Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
Survivin, Polyclonal Antibody (Cat# AAA22246)
Full Name
Survivin Polyclonal Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(LALBA Antibody (Center) (AAA28660)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
LALBA, Polyclonal Antibody (Cat# AAA28660)
Full Name
LALBA Antibody (Center)
Reactivity
Human
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Mouse Heart Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse MAP1A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Microtubule Associated Protein 1A, Polyclonal Antibody (Cat# AAA20871)
Full Name
Polyclonal Antibody to Microtubule Associated Protein 1A (MAP1A)
Gene Names
Map1a; Mtap1; moth1; Mtap-1; Mtap1a; AI853608; 6330416M19Rik
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD68, Polyclonal Antibody (Cat# AAA29179)
Full Name
CD68 Polyclonal Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of CACO-2 cells using anti-Galectin 2/LGALS2 antibody (AAA19287).Overlay histogram showing CACO-2 cells stained with AAA19287 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin 2/LGALS2 Antibody (AAA19287, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Galectin 2/LGALS2, Polyclonal Antibody (Cat# AAA19287)
Full Name
Anti-Galectin 2/LGALS2 Antibody
Gene Names
LGALS2; HL14
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-SLC6A2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human heart)
SLC6A2, Polyclonal Antibody (Cat# AAA23496)
Full Name
SLC6A2 antibody - middle region
Gene Names
SLC6A2; NET; NAT1; NET1; SLC6A5
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
Application Data
(C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)
FGFR2, Polyclonal Antibody (Cat# AAA26856)
Full Name
FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (PE)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS
Purity
Purified by Protein G Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using CUL4B antibody.)
CUL4B, Polyclonal Antibody (Cat# AAA28142)
Full Name
CUL4B Polyclonal Antibody
Gene Names
CUL4B; SFM2; MRXSC; CUL-4B; MRXHF2; MRXS15
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
p53, Polyclonal Antibody (Cat# AAA28404)
Full Name
[KO Validated] p53 Rabbit pAb
Gene Names
CDC45; CDC45L; CDC45L2; PORC-PI-1
Reactivity
Human
Applications
WB, IHC, IF, ICC, IP, ChIP
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of THP-1 cells using anti-Alpha B Crystallin/CRYAB antibody (AAA19277).Overlay histogram showing THP-1 cells stained with AAA19277 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha B Crystallin/CRYAB Antibody (AAA19277, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Alpha B Crystallin/CRYAB, Polyclonal Antibody (Cat# AAA19277)
Full Name
Anti-Alpha B Crystallin/CRYAB Antibody
Gene Names
CRYAB; CRYA2; CTPP2; HSPB5; CMD1II
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-THAP11 antibody (AAA19599).Overlay histogram showing MCF-7 cells stained with AAA19599 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THAP11 Antibody (AAA19599, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
THAP11, Polyclonal Antibody (Cat# AAA19599)
Full Name
Anti-THAP11 Antibody Picoband
Gene Names
THAP11; RONIN; CTG-B43a; CTG-B45d; HRIHFB2206
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-CHAF1B Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Human Liver)
CHAF1B, Polyclonal Antibody (Cat# AAA23522)
Full Name
CHAF1B antibody - N-terminal region
Gene Names
CHAF1B; CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Gelsolin, Polyclonal Antibody (Cat# AAA22263)
Full Name
Gelsolin Polyclonal Antibody
Gene Names
GSN; ADF; AGEL
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of 293T cells using anti-VPRBP/DCAF1 antibody (AAA19647).Overlay histogram showing 293T cells stained with AAA19647 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPRBP/DCAF1 Antibody (AAA19647, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
VPRBP/DCAF1, Polyclonal Antibody (Cat# AAA19647)
Full Name
Anti-VPRBP/DCAF1 Antibody Picoband
Gene Names
DCAF1; RIP; VPRBP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot;Sample: Mouse Serum.)
Complement Component 9, Polyclonal Antibody (Cat# AAA20913)
Full Name
Polyclonal Antibody to Complement Component 9 (C9)
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue)
Ficolin 2, Polyclonal Antibody (Cat# AAA20931)
Full Name
Polyclonal Antibody to Ficolin 2 (FCN2)
Gene Names
Fcnb; Fcn2
Reactivity
Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation MCF7(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-HDAC1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.)
HDAC1, Polyclonal Antibody (Cat# AAA18851)
Full Name
HDAC1 Antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Reactivity
Human
Applications
EIA, WB, IHC, ChIP
Purity
>95%, Protein G Purified
Pricing
WB (Western Blot)
(Western blot analysis of EYFP recombinant protein, using AAA29667 diluted at 1:5,000.)
EYFP, Polyclonal Antibody (Cat# AAA29667)
Full Name
EYFP Rabbit Polyclonal Antibody
Applications
WB
Purity
Affinity purification using immunogen.
Pricing
WB (Western Blot)
(Western Blot; Sample: Recombinant OXTR, Rat.)
Oxytocin Receptor, Polyclonal Antibody (Cat# AAA20861)
Full Name
Polyclonal Antibody to Oxytocin Receptor (OXTR)
Gene Names
Oxtr; OTR; OT-R; OTR1
Reactivity
Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Western Blot:Sample:Lane 1: Rat Liver Tissue;Lane 2: Mouse Brain Tissue; Lane 3: Rat Brain Tissue.)
Major Basic Protein (MBP), Polyclonal Antibody (Cat# AAA20127)
Full Name
Polyclonal Antibody to Major Basic Protein (MBP)
Gene Names
Prg2; MBP; EMBP; mMBP; mMBP-1
Reactivity
Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 18. Flow Cytometry analysis of A431 cells using anti-LSM7 antibody (AAA19327).Overlay histogram showing A431 cells stained with AAA19327 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LSM7 Antibody (AAA19327, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LSM7, Polyclonal Antibody (Cat# AAA19327)
Full Name
Anti-LSM7 Antibody
Gene Names
LSM7; YNL147W
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-Annexin A10/ANXA10 antibody (AAA19637).Overlay histogram showing PC-3 cells stained with AAA19637 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin A10/ANXA10 Antibody (AAA19637, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Annexin A10/ANXA10, Polyclonal Antibody (Cat# AAA19637)
Full Name
Anti-Annexin A10/ANXA10 Antibody Picoband
Gene Names
ANXA10; ANX14
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of K562 cells using anti-PACSIN1 antibody (AAA19863).Overlay histogram showing K562 cells stained with AAA19863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN1 Antibody (AAA19863, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PACSIN1, Polyclonal Antibody (Cat# AAA19863)
Full Name
Anti-PACSIN1 Antibody Picoband
Gene Names
PACSIN1; SDPI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-MAP4K3 antibody (AAA19882).Overlay histogram showing 293T cells stained with AAA19882 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP4K3 Antibody (AAA19882, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MAP4K3, Polyclonal Antibody (Cat# AAA19882)
Full Name
Anti-MAP4K3 Antibody Picoband
Gene Names
MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] JAK1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
JAK1, Polyclonal Antibody (Cat# AAA28369)
Full Name
[KO Validated] JAK1 Rabbit pAb
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using H2AFY antibody. Blue: DAPI for nuclear staining.)
H2AFY, Polyclonal Antibody (Cat# AAA28109)
Full Name
H2AFY Polyclonal Antibody
Gene Names
H2AFY; H2A.y; H2A/y; H2AFJ; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-SLC15A4 Antibody Titration: 0.25ug/mlPositive Control: HepG2 cell lysate)
SLC15A4, Polyclonal Antibody (Cat# AAA23514)
Full Name
SLC15A4 antibody - N-terminal region
Gene Names
SLC15A4; PHT1; PTR4; FP12591
Reactivity
Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using IMPA1 antibody at dilution of 1:100 (40x lens).)
IMPA1, Polyclonal Antibody (Cat# AAA28116)
Full Name
IMPA1 Polyclonal Antibody
Gene Names
IMPA1; IMP; IMPA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Catalase, Polyclonal Antibody (Cat# AAA29160)
Full Name
Catalase Polyclonal Antibody
Reactivity
Human
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-CD93 antibody (AAA19531).Overlay histogram showing U937 cells stained with AAA19531 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD93 Antibody (AAA19531, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD93, Polyclonal Antibody (Cat# AAA19531)
Full Name
Anti-CD93 Antibody Picoband
Gene Names
CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of RFX1 using anti-RFX1 antibody (AAA19814).RFX1 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
RFX1, Polyclonal Antibody (Cat# AAA19814)
Full Name
Anti-RFX1 Antibody Picoband
Gene Names
RFX1; EFC; RFX
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using AAA18535 at dilution of 1:100)
COLGALT2, Polyclonal Antibody (Cat# AAA18535)
Full Name
COLGALT2 Antibody
Gene Names
COLGALT2; C1orf17; GLT25D2
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SNAI1 Antibody Titration: 1ug/mlPositive Control: 721_B cell lysateSNAI1 is supported by BioGPS gene expression data to be expressed in 721_B)
SNAI1, Polyclonal Antibody (Cat# AAA23422)
Full Name
SNAI1 antibody - N-terminal region
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Guinea Pig, Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.