Polyclonal Antibodies

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Caco-2 cells using anti-BCCIP antibody (AAA19854).Overlay histogram showing Caco-2 cells stained with AAA19854 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCCIP Antibody (AAA19854, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of BCCIP using anti-BCCIP antibody (AAA19854).BCCIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCCIP antigen affinity purified polyclonal antibody (#AAA19854) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCCIP at approximately 45 kDa. The expected band size for BCCIP is at 36 kDa.)

BCCIP, Polyclonal Antibody (Cat# AAA19854)

Full Name

Anti-BCCIP Antibody Picoband

Gene Names

BCCIP; TOK1; TOK-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

C4orf52, Polyclonal Antibody (Cat# AAA28817)

Full Name

C4orf52 Polyclonal Antibody

Gene Names

SMIM20; C4orf52

Reactivity

Rat, Dog, Cow

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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XRCC6, Polyclonal Antibody (Cat# AAA10642)

Full Name

XRCC6 Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-PYCR1 antibody (AAA19858).Overlay histogram showing 293T cells stained with AAA19858 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR1 Antibody (AAA19858, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PYCR1, Polyclonal Antibody (Cat# AAA19858)

Full Name

Anti-PYCR1 Antibody Picoband

Gene Names

PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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ICC (Immunocytochemistry) (Cell culture(SW480); Paraformaldehyde-fixed; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Gamma-Adaptin) Polyclonal Antibody, Unconjugated (bs-1600R) at 1:200 overnight at 4 degree C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.)

Gamma-Adaptin, Polyclonal Antibody (Cat# AAA28818)

Full Name

Gamma-Adaptin Polyclonal Antibody

Gene Names

Ap1g1; Adtg; AA409002; AU041323; AW551707; D8Ertd374e

Reactivity

Rat, Pig, Dog, Cow

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

CYFIP2, Polyclonal Antibody (Cat# AAA29330)

Full Name

CYFIP2 Polyclonal Antibody

Gene Names

CYFIP2; PIR121

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

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WB (Western Blot) (WB Suggested Anti-CACNB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate)

CACNB1, Polyclonal Antibody (Cat# AAA23439)

Full Name

CACNB1 antibody - N-terminal region

Gene Names

CACNB1; CAB1; CCHLB1; CACNLB1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

TLE1/2/3/4, Polyclonal Antibody (Cat# AAA29327)

Full Name

TLE1/2/3/4 Polyclonal Antibody

Gene Names

TLE1; ESG; ESG1; GRG1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using PGK2 at dilution of 1:100 (40x lens).)

PGK2, Polyclonal Antibody (Cat# AAA30610)

Full Name

PGK2 Rabbit Polyclonal Antibody

Gene Names

PGK2; PGKB; PGKPS; HEL-S-272; dJ417L20.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using TJP2 antibody at dilution of 1:100 (40x lens).)

TJP2, Polyclonal Antibody (Cat# AAA10643)

Full Name

TJP2 Polyclonal Antibody

Gene Names

TJP2; ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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CYP1A1, Polyclonal Antibody (Cat# AAA11667)

Full Name

Anti-CYP1A1 Antibody

Gene Names

CYP1A1; AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Flow Cytometry (FC)

Purity

Immunogen Affinity Purified

Pricing

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Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck, Polyclonal Antibody (Cat# AAA14227)

Full Name

Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1)

Gene Names

UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5

Reactivity

Bovine, Human, Mouse, Pig, Rat
Expected Reactivity: Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Total IgY fraction

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)

E-Selectin, Polyclonal Antibody (Cat# AAA29326)

Full Name

E-Selectin Polyclonal Antibody

Gene Names

SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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CCAR2, Polyclonal Antibody (Cat# AAA29838)

Full Name

CCAR2 Polyclonal Antibody

Gene Names

KIAA1967; DBC1; DBC-1; NET35; p30DBC; p30 DBC

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using BRCA1 antibody.)

BRCA1, Polyclonal Antibody (Cat# AAA10639)

Full Name

BRCA1 Polyclonal Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-MIPEP antibody (AAA19856).Overlay histogram showing MCF-7 cells stained with AAA19856 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIPEP Antibody (AAA19856, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MIPEP using anti-MIPEP antibody (AAA19856).MIPEP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIPEP antigen affinity purified polyclonal antibody (#AAA19856) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIPEP at approximately 80 kDa. The expected band size for MIPEP is at 80 kDa.)

MIPEP, Polyclonal Antibody (Cat# AAA19856)

Full Name

Anti-MIPEP Antibody Picoband

Gene Names

MIPEP; MIP; HMIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti-MMS19 antibody (AAA19865).Overlay histogram showing Hela cells stained with AAA19865 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMS19 Antibody (AAA19865, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MMS19 using anti-MMS19 antibody (AAA19865).MMS19 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MMS19 Antibody (AAA19865) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMS19 antigen affinity purified polyclonal antibody (#AAA19865) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMS19 at approximately 113 kDa. The expected band size for MMS19 is at 113 kDa.)

MMS19, Polyclonal Antibody (Cat# AAA19865)

Full Name

Anti-MMS19 Antibody Picoband

Gene Names

MMS19; MET18; MMS19L; hMMS19

Reactivity

Human, Mouse

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-ESR2 antibody Titration: 1 ug/mLSample Type: Human liver)

ESR2, Polyclonal Antibody (Cat# AAA23449)

Full Name

ESR2 antibody - N-terminal region

Gene Names

ESR2; Erb; ESRB; ODG8; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)

Acetyl-Histone H4-K12, Polyclonal Antibody (Cat# AAA28313)

Full Name

Acetyl-Histone H4-K12 Rabbit pAb

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

NOS2, Polyclonal Antibody (Cat# AAA29081)

Full Name

NOS2 Polyclonal Antibody

Gene Names

NOS2; NOS; INOS; NOS2A; HEP-NOS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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WB (Western Blot) (WB Suggested Anti-PRDM9 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysate)

PRDM9, Polyclonal Antibody (Cat# AAA23451)

Full Name

PRDM9 antibody - middle region

Gene Names

PRDM9; PFM6; KMT8B; MSBP3; ZNF899; MEISETZ

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

NY-CO-1, Polyclonal Antibody (Cat# AAA29083)

Full Name

NY-CO-1 Polyclonal Antibody

Gene Names

NEMF; NY-CO-1; SDCCAG1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

CLECSF6, Polyclonal Antibody (Cat# AAA29339)

Full Name

CLECSF6 Polyclonal Antibody

Gene Names

CLEC4A; DCIR; LLIR; DDB27; CLECSF6; HDCGC13P

Reactivity

Human

Applications

Western Blot (WB)

Pricing

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SUZ12, Polyclonal Antibody (Cat# AAA29851)

Full Name

SUZ12 Polyclonal Antibody

Gene Names

SUZ12; CHET9; JJAZ1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

Collagen 1, alpha 1 telopeptide, Polyclonal Antibody (Cat# AAA14236)

Full Name

Anti-Collagen I alpha1 Telopeptide Sequence

Gene Names

COL1A1; OI4

Reactivity

Human, mouse, sheep

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

IHC (Immunohistochemistry) (Figure 7. IHC analysis of OCM using anti-OCM antibody (AAA19866).OCM was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OCM Antibody (AAA19866) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

OCM, Polyclonal Antibody (Cat# AAA19866)

Full Name

Anti-OCM Antibody

Gene Names

OCM; OM; OCM1; ONCM

Reactivity

Human

Applications

Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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YBX1, Polyclonal Antibody (Cat# AAA29850)

Full Name

YBX1 Polyclonal Antibody

Gene Names

YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

MAX, Polyclonal Antibody (Cat# AAA31386)

Full Name

Phospho-MAX (Ser11) Antibody

Gene Names

MAX; bHLHd4

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (83%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Jurkat cells using anti-SUCLG1 antibody (AAA19867).Overlay histogram showing Jurkat cells stained with AAA19867 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG1 Antibody (AAA19867, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SUCLG1 using anti-SUCLG1 antibody (AAA19867).SUCLG1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (#AAA19867) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 35 kDa. The expected band size for SUCLG1 is at 36 kDa.)

SUCLG1, Polyclonal Antibody (Cat# AAA19867)

Full Name

Anti-SUCLG1 Antibody Picoband

Gene Names

SUCLG1; GALPHA; MTDPS9; SUCLA1

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-SSX4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

SSX4, Polyclonal Antibody (Cat# AAA23446)

Full Name

SSX4 antibody - middle region

Gene Names

SSX4; CT5.4

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

N4BP1, Polyclonal Antibody (Cat# AAA29078)

Full Name

N4BP1 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GNB2L1 at 1:1000 dilution.)

GNB2L1, Polyclonal Antibody (Cat# AAA30614)

Full Name

GNB2L1 Rabbit Polyclonal Antibody

Gene Names

GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1

Reactivity

Human

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using FGF2 antibody at dilution of 1:100 (40x lens).)

FGF2, Polyclonal Antibody (Cat# AAA10647)

Full Name

FGF2 Polyclonal Antibody

Gene Names

FGF2; BFGF; FGFB; FGF-2; HBGF-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

RbAp48, Polyclonal Antibody (Cat# AAA11671)

Full Name

Anti-RbAp48 Antibody

Gene Names

RBBP4; NURF55; RBAP48; lin-53

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

HBcAg (HBV), Polyclonal Antibody (Cat# AAA13719)

Full Name

Anti-HBcAg (HBV), rabbit IgG

Applications

Western Blot (WB), ELISA (EIA)

Purity

Immunoaffinity chromatography

Pricing

Interferon-a Receptor, Type I, Subunit I (Ser535,539), Polyclonal Antibody (Cat# AAA14231)

Full Name

Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I

Gene Names

IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC

Reactivity

Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

NF2, Polyclonal Antibody (Cat# AAA29080)

Full Name

NF2 Polyclonal Antibody

Gene Names

NF2; ACN; SCH; BANF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

HSL, Polyclonal Antibody (Cat# AAA31384)

Full Name

Phospho-HSL (Ser660) Antibody

Gene Names

LIPE; HSL; LHS

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (88%), Horse (89%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PACSIN1 antibody (AAA19863).Overlay histogram showing K562 cells stained with AAA19863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN1 Antibody (AAA19863, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PACSIN1 using anti-PACSIN1 antibody (AAA19863) and anti-Beta Tubulin antibody (M01857-3).PACSIN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN1 antigen affinity purified polyclonal antibody (#AAA19863) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PACSIN1 at approximately 51 kDa. The expected band size for PACSIN1 is at 51 kDa.)

PACSIN1, Polyclonal Antibody (Cat# AAA19863)

Full Name

Anti-PACSIN1 Antibody Picoband

Gene Names

PACSIN1; SDPI

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-ZNF238 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Pancreas)

ZNF238, Polyclonal Antibody (Cat# AAA23447)

Full Name

ZNF238 antibody - N-terminal region

Gene Names

ZBTB18; RP58; MRD22; TAZ-1; ZNF238; C2H2-171

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)

Ret, Polyclonal Antibody (Cat# AAA29335)

Full Name

Ret Polyclonal Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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TCP1 delta, Polyclonal Antibody (Cat# AAA11672)

Full Name

Anti-TCP1 delta Antibody

Gene Names

CCT4; SRB; Cctd; CCT-DELTA

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

NMDA NR2A Subunit, Polyclonal Antibody (Cat# AAA14232)

Full Name

Anti-NMDA Receptor, NR2A Subunit

Gene Names

Grin2a; NR2A; GluN2A; NMDAR2A

Reactivity

Rat, mouse, human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse heart cells using LDB3 antibody.)

LDB3, Polyclonal Antibody (Cat# AAA29845)

Full Name

LDB3 Polyclonal Antibody

Gene Names

LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells using TEAD1.)

TEAD1, Polyclonal Antibody (Cat# AAA30613)

Full Name

TEAD1 Rabbit Polyclonal Antibody

Gene Names

TEAD1; AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western Blot of rat brain lysate showing specific immunolabeling of SHANK1 at 1:500 dilution.)

SHANK1a, Polyclonal Antibody (Cat# AAA14230)

Full Name

Anti-SHANK1a (C-terminus) Antibody

Gene Names

SHANK1; SSTRIP; SPANK-1; synamon

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)