Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HL-60 cells using anti-RNF34 antibody (A07903-1).Overlay histogram showing HL-60 cells stained with A07903-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF34 Antibody (A07903-1, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RNF34 and Tubulin beta using anti-RNF34 antibody (A07903-1) and anti-Tubulin beta antibody (M05613-4).RNF34 and Tubulin beta was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF34 Antibody (A07903-1) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF34 antigen affinity purified polyclonal antibody (#AAA19899) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RNF34 at approximately 42 kDa. The expected band size for RNF34 is at 42 kDa.)

RNF34, Polyclonal Antibody (Cat# AAA19899)

Full Name

Anti-RNF34 Antibody Picoband

Gene Names

RNF34; RFI; RIF; RIFF; hRFI; CARP1; CARP-1

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of A-549 cells usingug PALLD Polyclonal Antibody.Western blot was performed from the immunoprecipitate using PALLD Polyclonal Antibody at a dilution of 1:1000.)

PALLD, Polyclonal Antibody (Cat# AAA22252)

Full Name

PALLD Polyclonal Antibody

Gene Names

PALLD; MYN; PNCA1; CGI151; SIH002; CGI-151

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HL-60 cells using anti-RPL32 antibody (AAA19570).Overlay histogram showing HL-60 cells stained with AAA19570 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (AAA19570, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RPL32, Polyclonal Antibody (Cat# AAA19570)

Full Name

Anti-RPL32 Antibody Picoband

Gene Names

RPL32; L32; PP9932

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

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Mucin 5 Subtype AC, Polyclonal Antibody (Cat# AAA20932)

Full Name

Polyclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

Gene Names

Muc5ac; MGM; 2210005L13Rik

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistchemistry) (DAB staining on IHC-P.Samples: Human Tissue)

RAD51 Homolog, Polyclonal Antibody (Cat# AAA21011)

Full Name

RAD51 Homolog (RAD51) Polyclonal Antibody

Gene Names

RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930

Reactivity

Human, Mouse, Rat

Applications

Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

GM-CSF-R-alpha, Polyclonal Antibody (Cat# AAA17988)

Full Name

GM-CSF-R-alpha (CD116) Antibody

Gene Names

Csf2ra; CD116; Csfgmra; GM-CSFR; GM-CSF-Ra

Reactivity

Mouse

Applications

WB, ICC

Purity

Affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HL-60 cells using anti-NLRX1 antibody (AAA19829).Overlay histogram showing HL-60 cells stained with AAA19829 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRX1 Antibody (AAA19829, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NLRX1 using anti-NLRX1 antibody (AAA19829).NLRX1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NLRX1 Antibody (AAA19829) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat skeletal muscle tissue lysates,Lane 7: mouse heart tissue lysates,Lane 8: mouse skeletal muscle tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRX1 antigen affinity purified polyclonal antibody (#AAA19829) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NLRX1 at approximately 108 kDa. The expected band size for NLRX1 is at 108 kDa.)

NLRX1, Polyclonal Antibody (Cat# AAA19829)

Full Name

Anti-NLRX1 Antibody Picoband

Gene Names

NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse spleen using LRRK2 Polyclonal Antibody at dilution of 1:200 (40x lens).)

LRRK2, Polyclonal Antibody (Cat# AAA22211)

Full Name

LRRK2 Polyclonal Antibody

Gene Names

LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN

Reactivity

Human, Mouse, Rat

Applications

IHC

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-KRT8 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)

KRT8, Polyclonal Antibody (Cat# AAA23498)

Full Name

KRT8 Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Yeast

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-RPS25 antibody (AAA19920).Overlay histogram showing HL-60 cells stained with AAA19920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS25 Antibody (AAA19920, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RPS25 using anti-RPS25 antibody (AAA19920).RPS25 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS25 antigen affinity purified polyclonal antibody (#AAA19920) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS25 at approximately 17 kDa. The expected band size for RPS25 is at 14 kDa.)

RPS25, Polyclonal Antibody (Cat# AAA19920)

Full Name

Anti-RPS25 Antibody Picoband

Gene Names

RPS25; S25

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

DIAPH3, Polyclonal Antibody (Cat# AAA29934)

Full Name

DIAPH3 Antibody

Gene Names

DIAPH3; AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2

Reactivity

Human, Mouse

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Stomach Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse GYPA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))

Glycophorin A, Polyclonal Antibody (Cat# AAA20961)

Full Name

Polyclonal Antibody to Glycophorin A (GYPA)

Gene Names

Gypa; GPA; CD235a; AI853584

Reactivity

Mouse

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RTCB antibody (AAA19833).Overlay histogram showing Hela cells stained with AAA19833 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCB Antibody (AAA19833, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RTCB using anti-RTCB antibody (AAA19833).RTCB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (#AAA19833) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.)

RTCB, Polyclonal Antibody (Cat# AAA19833)

Full Name

Anti-RTCB Antibody Picoband

Gene Names

RTCB; FAAP; HSPC117; C22orf28; DJ149A16.6

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using TARDBP Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

TARDBP, Polyclonal Antibody (Cat# AAA22208)

Full Name

TARDBP Polyclonal Antibody

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of U87 cells using anti-RBMS1 antibody (AAA19911).Overlay histogram showing U87 cells stained with AAA19911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBMS1 Antibody (AAA19911, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of RBMS1 using anti-RBMS1 antibody (AAA19911).RBMS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS1 antigen affinity purified polyclonal antibody (#AAA19911) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBMS1 at approximately 50 kDa. The expected band size for RBMS1 is at 45 kDa.)

RBMS1, Polyclonal Antibody (Cat# AAA19911)

Full Name

Anti-RBMS1 Antibody Picoband

Gene Names

RBMS1; YC1; MSSP; SCR2; HCC-4; MSSP-1; MSSP-2; MSSP-3; C2orf12

Reactivity

Human, Mouse

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

DLL3, Polyclonal Antibody (Cat# AAA26850)

Full Name

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)

Gene Names

DLL3; SCDO1

Reactivity

Human

Applications

IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

GABA A Receptor alpha4, Polyclonal Antibody (Cat# AAA28898)

Full Name

GABA A Receptor alpha4 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of GSTM3 using anti-GSTM3 antibody (AAA19168).GSTM3 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSTM3 Antibody (AAA19168) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GSTM3, Polyclonal Antibody (Cat# AAA19168)

Full Name

Anti-GSTM3 Picoband antibody

Gene Names

GSTM3; GST5; GSTB; GTM3; GSTM3-3

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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PCK1, Polyclonal Antibody (Cat# AAA23418)

Full Name

PCK1 antibody - middle region

Gene Names

PCK1; PCKDC; PEPCK1; PEPCKC; PEPCK-C

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using GSTA1 Polycloanl Antibody at dilution of 1:200)

GSTA1, Polyclonal Antibody (Cat# AAA22171)

Full Name

GSTA1 Polyclonal Antibody

Gene Names

Gsta1; Gst2-1; OTTMUSG00000031890

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Antigen affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-FOXO3 Antibody Titration: 0.2-1 ug/mlPositive Control: Transfected 293T)

FOXO3, Polyclonal Antibody (Cat# AAA23460)

Full Name

FOXO3 antibody - N-terminal region

Gene Names

FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Applications

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2OS cells using Caspase-3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Caspase-3, Polyclonal Antibody (Cat# AAA22200)

Full Name

Caspase-3 Polyclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)

ZBTB48, Polyclonal Antibody (Cat# AAA29927)

Full Name

ZBTB48 Antibody

Gene Names

ZBTB48; HKR3; ZNF855; pp9964

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse testis cells using DDX4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

DDX4, Polyclonal Antibody (Cat# AAA22255)

Full Name

DDX4 Polyclonal Antibody

Gene Names

DDX4; VASA

Reactivity

Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

S100B, Polyclonal Antibody (Cat# AAA29687)

Full Name

S100B Antibody

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human Mouse Rat

Applications

IHC

Purity

Antigen affinity purification.

Pricing

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BCKDK, Polyclonal Antibody (Cat# AAA19589)

Full Name

Anti-BCKDK Antibody Picoband

Gene Names

BCKDK; BDK; BCKDKD

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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TRMT6, Polyclonal Antibody (Cat# AAA19634)

Full Name

Anti-TRMT6 Antibody Picoband

Gene Names

TRMT6; GCD10; CGI-09; Gcd10p

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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GNG7, Polyclonal Antibody (Cat# AAA19335)

Full Name

Anti-GNG7 Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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anti-Human IgM, Polyclonal Antibody (Cat# AAA14450)

Full Name

Affinity Purified Goat anti-Human IgM

Reactivity

Human

Purity

Antigen Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-HNRPUL1 Antibody Titration: 0.25ug/mlELISA Titer: 1:312500Positive Control: Raji cell lysateHNRNPUL1 is supported by BioGPS gene expression data to be expressed in Raji)

HNRPUL1, Polyclonal Antibody (Cat# AAA23483)

Full Name

HNRPUL1 antibody - C-terminal region

Gene Names

HNRNPUL1; E1BAP5; E1B-AP5; HNRPUL1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

IF, IP, IHC, WB

Purity

Protein A purified

Pricing

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WB (Western Blot) (WB Suggested Anti-POSTN AntibodyTitration: 1 ug/mlPositive Control: 293T cells lysate)

POSTN, Polyclonal Antibody (Cat# AAA23493)

Full Name

POSTN antibody - middle region

Gene Names

POSTN; PN; OSF2; OSF-2; PDLPOSTN

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-SLC39A5 Antibody Titration: 0.5ug/mlPositive Control: HepG2 cell lysate)

SLC39A5, Polyclonal Antibody (Cat# AAA23515)

Full Name

SLC39A5 antibody - N-terminal region

Gene Names

SLC39A5; ZIP5; MYP24; LZT-Hs7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Cbx8 using anti-Cbx8 antibody (AAA19304).Cbx8 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cbx8 Antibody (AAA19304) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

Cbx8, Polyclonal Antibody (Cat# AAA19304)

Full Name

Anti-Cbx8 Antibody

Gene Names

CBX8; PC3; RC1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

SLC12A4, Polyclonal Antibody (Cat# AAA28903)

Full Name

SLC12A4 Polyclonal Antibody

Gene Names

SLC12A4; KCC1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using HACE1 at dilution of 1:100 (40x lens).)

HACE1, Polyclonal Antibody (Cat# AAA30714)

Full Name

HACE1 Rabbit Polyclonal Antibody

Gene Names

HACE1; SPPRS

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Cdk4, Polyclonal Antibody (Cat# AAA29176)

Full Name

Cdk4 Polyclonal Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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PRPF3, Polyclonal Antibody (Cat# AAA28150)

Full Name

PRPF3 Polyclonal Antibody

Gene Names

PRPF3; PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-RAB27B antibody (AAA19825).Overlay histogram showing PC-3 cells stained with AAA19825 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB27B Antibody (AAA19825, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of RAB27B using anti-RAB27B antibody (AAA19825).RAB27B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB27B Antibody (AAA19825) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB27B Antibody (AAA19825) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB27B Antibody (AAA19825) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB27B Antibody (AAA19825) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: rat stomach tissue lysates,Lane 5: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27B antigen affinity purified polyclonal antibody (#AAA19825) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB27B at approximately 25 kDa. The expected band size for RAB27B is at 25 kDa.)

RAB27B, Polyclonal Antibody (Cat# AAA19825)

Full Name

Anti-RAB27B Antibody Picoband

Gene Names

RAB27B; C25KG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistochemistry) (Figure 7. IHC analysis of IRF2BPL using anti-IRF2BPL antibody (AAA19950).IRF2BPL was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IRF2BPL Antibody (AAA19950) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human MCF-7whole cell lysates,Lane 4: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2BPL antigen affinity purified polyclonal antibody (#AAA19950) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IRF2BPL at approximately 95 kDa. The expected band size for IRF2BPL is at 83 kDa.)

IRF2BPL, Polyclonal Antibody (Cat# AAA19950)

Full Name

Anti-IRF2BPL Antibody Picoband

Gene Names

IRF2BPL; EAP1; C14orf4

Reactivity

Human

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (ID1 Antibody (Center) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ID1, Polyclonal Antibody (Cat# AAA28652)

Full Name

ID1 Antibody (Center)

Gene Names

ID1; ID; bHLHb24

Reactivity

Human, mouse (Predicted Reactivity: Rat)

Applications

WB, EIA, IHC, IF, FC/FACS

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Carbonic Anhydrase 13/CA13 antibody (AAA19326).Overlay histogram showing CACO-2 cells stained with AAA19326 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Carbonic Anhydrase 13/CA13 Antibody (AAA19326, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Carbonic Anhydrase 13/CA13, Polyclonal Antibody (Cat# AAA19326)

Full Name

Anti-Carbonic Anhydrase 13/CA13 Antibody

Gene Names

CA13; CAXIII

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Figure 6. IF analysis of GHITM using anti-GHITM antibody (AAA19621).GHITM was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GHITM Antibody (AAA19621) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

GHITM, Polyclonal Antibody (Cat# AAA19621)

Full Name

Anti-GHITM Antibody Picoband

Gene Names

GHITM; DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-TCF21 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Lung)

TCF21, Polyclonal Antibody (Cat# AAA23424)

Full Name

TCF21 antibody - N-terminal region

Gene Names

TCF21; POD1; bHLHa23

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of 293T cells using anti-NVL antibody (AAA19846).Overlay histogram showing 293T cells stained with AAA19846 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NVL Antibody (AAA19846, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of NVL using anti-NVL antibody (AAA19846) and anti-Beta Tubulin antibody (M01857-3).NVL was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NVL Antibody (AAA19846) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NVL antigen affinity purified polyclonal antibody (#AAA19846) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NVL at approximately 95 kDa. The expected band size for NVL is at 95 kDa.)

NVL, Polyclonal Antibody (Cat# AAA19846)

Full Name

Anti-NVL Antibody Picoband

Reactivity

Human, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-GAPDH antibody Titration: 1 ug/mLSample Type: Human Raji)

GAPDH, Polyclonal Antibody (Cat# AAA23468)

Full Name

GAPDH antibody - N-terminal region

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-PUF60 Antibody Titration: 0.2-1 ug/mlPositive Control: Hela cell lysatePUF60 is supported by BioGPS gene expression data to be expressed in HeLa)

PUF60, Polyclonal Antibody (Cat# AAA23485)

Full Name

PUF60 antibody - C-terminal region

Gene Names

PUF60; FIR; VRJS; RoBPI; SIAHBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-RHOT1 antibody Titration: 1 ug/mLSample Type: Human A549)

RHOT1, Polyclonal Antibody (Cat# AAA23519)

Full Name

RHOT1 antibody - N-terminal region

Gene Names

RHOT1; ARHT1; MIRO1; MIRO-1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CyPA, Polyclonal Antibody (Cat# AAA29175)

Full Name

CyPA Polyclonal Antibody

Gene Names

PPIA; CYPA; CYPH

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

HoxD3, Polyclonal Antibody (Cat# AAA29173)

Full Name

HoxD3 Polyclonal Antibody

Gene Names

HOXD3; HOX4; HOX1D; HOX4A; Hox-4.1; MGC10470; HOXD3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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PDIA5, Polyclonal Antibody (Cat# AAA19331)

Full Name

Anti-PDIA5 Antibody

Gene Names

PDIA5; PDIR

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 2600-2650 of 10000 product results

RNF34, Polyclonal Antibody (Cat# AAA19899)

PALLD, Polyclonal Antibody (Cat# AAA22252)

RPL32, Polyclonal Antibody (Cat# AAA19570)

Mucin 5 Subtype AC, Polyclonal Antibody (Cat# AAA20932)

RAD51 Homolog, Polyclonal Antibody (Cat# AAA21011)

GM-CSF-R-alpha, Polyclonal Antibody (Cat# AAA17988)

NLRX1, Polyclonal Antibody (Cat# AAA19829)

LRRK2, Polyclonal Antibody (Cat# AAA22211)

KRT8, Polyclonal Antibody (Cat# AAA23498)

RPS25, Polyclonal Antibody (Cat# AAA19920)

DIAPH3, Polyclonal Antibody (Cat# AAA29934)

Glycophorin A, Polyclonal Antibody (Cat# AAA20961)

RTCB, Polyclonal Antibody (Cat# AAA19833)

TARDBP, Polyclonal Antibody (Cat# AAA22208)

RBMS1, Polyclonal Antibody (Cat# AAA19911)

DLL3, Polyclonal Antibody (Cat# AAA26850)

GABA A Receptor alpha4, Polyclonal Antibody (Cat# AAA28898)

GSTM3, Polyclonal Antibody (Cat# AAA19168)

PCK1, Polyclonal Antibody (Cat# AAA23418)

GSTA1, Polyclonal Antibody (Cat# AAA22171)

FOXO3, Polyclonal Antibody (Cat# AAA23460)

Caspase-3, Polyclonal Antibody (Cat# AAA22200)

ZBTB48, Polyclonal Antibody (Cat# AAA29927)

DDX4, Polyclonal Antibody (Cat# AAA22255)

S100B, Polyclonal Antibody (Cat# AAA29687)

BCKDK, Polyclonal Antibody (Cat# AAA19589)

TRMT6, Polyclonal Antibody (Cat# AAA19634)

GNG7, Polyclonal Antibody (Cat# AAA19335)

anti-Human IgM, Polyclonal Antibody (Cat# AAA14450)

HNRPUL1, Polyclonal Antibody (Cat# AAA23483)

POSTN, Polyclonal Antibody (Cat# AAA23493)

SLC39A5, Polyclonal Antibody (Cat# AAA23515)

Cbx8, Polyclonal Antibody (Cat# AAA19304)

SLC12A4, Polyclonal Antibody (Cat# AAA28903)

HACE1, Polyclonal Antibody (Cat# AAA30714)

Cdk4, Polyclonal Antibody (Cat# AAA29176)

PRPF3, Polyclonal Antibody (Cat# AAA28150)

RAB27B, Polyclonal Antibody (Cat# AAA19825)

IRF2BPL, Polyclonal Antibody (Cat# AAA19950)

ID1, Polyclonal Antibody (Cat# AAA28652)

Carbonic Anhydrase 13/CA13, Polyclonal Antibody (Cat# AAA19326)

GHITM, Polyclonal Antibody (Cat# AAA19621)

TCF21, Polyclonal Antibody (Cat# AAA23424)

NVL, Polyclonal Antibody (Cat# AAA19846)

GAPDH, Polyclonal Antibody (Cat# AAA23468)

PUF60, Polyclonal Antibody (Cat# AAA23485)

RHOT1, Polyclonal Antibody (Cat# AAA23519)

CyPA, Polyclonal Antibody (Cat# AAA29175)

HoxD3, Polyclonal Antibody (Cat# AAA29173)

PDIA5, Polyclonal Antibody (Cat# AAA19331)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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