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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2800-2850 of 10000 product results
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEPG2 cells using anti-ALDH1L1 antibody (AAA19297).Overlay histogram showing HEPG2 cells stained with AAA19297 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1L1 Antibody (AAA19297 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ALDH1L1, Polyclonal Antibody (Cat# AAA19297)
Full Name
Anti-ALDH1L1 Antibody
Gene Names
ALDH1L1; FDH; FTHFD; 10-fTHF; 10-FTHFDH
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of HL-60 cells using anti-RAB3GAP1 antibody (AAA19824).Overlay histogram showing HL-60 cells stained with AAA19824 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB3GAP1 Antibody (AAA19824, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RAB3GAP1, Polyclonal Antibody (Cat# AAA19824)
Full Name
Anti-RAB3GAP1 Antibody Picoband
Gene Names
RAB3GAP1; P130; WARBM1; RAB3GAP; RAB3GAP130
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ESRRA Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
ESRRA, Polyclonal Antibody (Cat# AAA22180)
Full Name
ESRRA Polyclonal Antibody
Gene Names
ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using CCDC59 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
CCDC59, Polyclonal Antibody (Cat# AAA22181)
Full Name
CCDC59 Polyclonal Antibody
Gene Names
CCDC59; BR22; TAP26; HSPC128
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of PLEKHA1 using anti-PLEKHA1 antibody (AAA19916).PLEKHA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PLEKHA1 Antibody (AAA19916) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PLEKHA1, Polyclonal Antibody (Cat# AAA19916)
Full Name
Anti-PLEKHA1 Antibody Picoband
Gene Names
PLEKHA1; TAPP1
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-SCLY antibody (AAA19917).Overlay histogram showing SH-SY5Y cells stained with AAA19917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCLY Antibody (AAA19917, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SCLY, Polyclonal Antibody (Cat# AAA19917)
Full Name
Anti-SCLY Antibody Picoband
Gene Names
SCLY; SCL; hSCL
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using SRP72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
SRP72, Polyclonal Antibody (Cat# AAA22251)
Full Name
SRP72 Polyclonal Antibody
Gene Names
SRP72; BMFF; BMFS1; HEL103
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P.Samples: Mouse Spleen TissuePrimary Ab: 20ug/ml Rabbit Anti-Mouse PR3 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))
Proteinase 3 (PR3), Polyclonal Antibody (Cat# AAA20162)
Full Name
Polyclonal Antibody to Proteinase 3 (PR3)
Gene Names
Prtn3; PR3; mPR3
Reactivity
Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HepG2 using NRF2 antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
NRF2, Polyclonal Antibody (Cat# AAA28410)
Full Name
NRF2 Rabbit pAb
Gene Names
LOC4339539; GPB-LR; RACK1B; OsJ_19467
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of LI Cadherin/CDH17 using anti-LI Cadherin/CDH17 antibody (AAA19569).LI Cadherin/CDH17 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-LI Cadherin/CDH17 Antibody (AAA19569) overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
LI Cadherin/CDH17, Polyclonal Antibody (Cat# AAA19569)
Full Name
Anti-LI Cadherin/CDH17 Antibody Picoband
Gene Names
CDH17; HPT1; CDH16; HPT-1
Reactivity
Human, Mouse
Applications
IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HepG2 cells using anti-TTC7B antibody (AAA19626).Overlay histogram showing HepG2 cells stained with AAA19626 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC7B Antibody (AAA19626, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TTC7B, Polyclonal Antibody (Cat# AAA19626)
Full Name
Anti-TTC7B Antibody Picoband
Gene Names
TTC7B; TTC7L1; c14_5685
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-PCK1 Antibody Titration: 1.0ug/mlPositive Control: Fetal Brain Lysate)
PCK1, Polyclonal Antibody (Cat# AAA23419)
Full Name
PCK1 antibody - middle region
Gene Names
PCK1; PCKDC; PEPCK1; PEPCKC; PEPCK-C
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
IHC, WB
Purity
Protein A purified
Pricing
WB (Western Blot)
(WB Suggested Anti-MOSPD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate)
MOSPD2, Polyclonal Antibody (Cat# AAA23548)
Full Name
MOSPD2 antibody - middle region
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Applications
WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
RYR2, Polyclonal Antibody (Cat# AAA29945)
Full Name
RYR2 Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of PC-3 cells using anti-TIGD1 antibody (AAA19643).Overlay histogram showing PC-3 cells stained with AAA19643 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGD1 Antibody (AAA19643, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TIGD1, Polyclonal Antibody (Cat# AAA19643)
Full Name
Anti-TIGD1 Antibody Picoband
Gene Names
TIGD1; EEYORE
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using ATF5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
ATF5, Polyclonal Antibody (Cat# AAA28366)
Full Name
ATF5 Rabbit pAb
Gene Names
ATF5; ATFX; HMFN0395
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-MED26 antibody (AAA19925).Overlay histogram showing 293T cells stained with AAA19925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED26 Antibody (AAA19925, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MED26, Polyclonal Antibody (Cat# AAA19925)
Full Name
Anti-MED26 Antibody Picoband
Gene Names
MED26; CRSP7; CRSP70
Reactivity
Human
Applications
EIA, IF, IHC, ICC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of SH-SY5Y cells using anti-IPO9 antibody (AAA19940).Overlay histogram showing SH-SY5Y cells stained with AAA19940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPO9 Antibody (AAA19940, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IPO9, Polyclonal Antibody (Cat# AAA19940)
Full Name
Anti-IPO9 Antibody Picoband
Gene Names
IPO9; Imp9
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Figure 7. Immunoprecipitating GLIPR2 in U251 whole cell lysate .Western blot analysis of GLIPR2 using anti-GLIPR2 antibody (AAA19941).Lane 1: U251 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-GLIPR2 antibody in U251 whole cell lysate.Lane 3: anti-GLIPR2 antibody (2ug) + U251 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GLIPR2 antigen affinity purified polyclonal antibody (AAA19941) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLIPR2 Antibody (AAA19941, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GLIPR2, Polyclonal Antibody (Cat# AAA19941)
Full Name
Anti-GLIPR2 Antibody Picoband
Gene Names
GLIPR2; GAPR1; GAPR-1; C9orf19
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IP, IHC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Chk2, Polyclonal Antibody (Cat# AAA28922)
Full Name
Chk2 (phospho Thr68) Polyclonal Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Lipocalin-1, Polyclonal Antibody (Cat# AAA29183)
Full Name
Lipocalin-1 Polyclonal Antibody
Gene Names
LCN1; TP; TLC; PMFA; VEGP
Reactivity
Human
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-HSF1 antibody (AAA19393).Overlay histogram showing MCF-7 cells stained with AAA19393 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSF1 Antibody (AAA19393, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HSF1, Polyclonal Antibody (Cat# AAA19393)
Full Name
Anti-HSF1 Antibody Picoband
Gene Names
HSF1; HSTF1
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using ACAT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
ACAT2, Polyclonal Antibody (Cat# AAA22221)
Full Name
ACAT2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-STRIP1 antibody (AAA19927).Overlay histogram showing SH-SY5Y cells stained with AAA19927 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STRIP1 Antibody (AAA19927, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STRIP1, Polyclonal Antibody (Cat# AAA19927)
Full Name
Anti-STRIP1 Antibody Picoband
Gene Names
STRIP1; FAM40A; FAR11A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody AAA23777 (lot AAA23777-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
PBRM1, Polyclonal Antibody (Cat# AAA23777)
Full Name
Rabbit anti-PBRM1 Antibody, Affinity Purified
Gene Names
PBRM1; PB1; BAF180
Reactivity
Human, Mouse
Applications
WB, IP, IHC
Purity
Antigen Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-LDHB AntibodyTitration: 1 ug/mlPositive Control: 721_B Whole CellLDHB is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
LDHB, Polyclonal Antibody (Cat# AAA23530)
Full Name
LDHB Antibody - C-terminal region
Gene Names
LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of RAW264.7 cells using anti-PAK1 antibody (AAA19397).Overlay histogram showing RAW264.7 cells stained with AAA19397 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAK1 Antibody (AAA19397, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PAK1, Polyclonal Antibody (Cat# AAA19397)
Full Name
Anti-PAK1 Antibody Picoband
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of SCRN1 using anti-SCRN1 antibody (AAA19614).SCRN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-SCRN1 Antibody (AAA19614) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SCRN1, Polyclonal Antibody (Cat# AAA19614)
Full Name
Anti-SCRN1 Antibody Picoband
Gene Names
SCRN1; SES1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 14. IF analysis of TTLL2 using anti-TTLL2 antibody (AAA19641).TTLL2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-TTLL2 Antibody (AAA19641) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
TTLL2, Polyclonal Antibody (Cat# AAA19641)
Full Name
Anti-TTLL2 Antibody Picoband
Gene Names
TTLL2; C6orf104; NYD-TSPG; dJ366N23.3
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-TGFB1 Antibody Titration: 0.2-1 ug/ml or 1:5000 to 1:1000 dilution.SP2/0 cell lysate)
TGFB1, Polyclonal Antibody (Cat# AAA23459)
Full Name
TGFB1 antibody - middle region
Gene Names
Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep (Tested Reactivity: Human, Mouse)
Applications
IHC, WB, IF
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Mouse pancreas using PNLIPRP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PNLIPRP1, Polyclonal Antibody (Cat# AAA22215)
Full Name
PNLIPRP1 Polyclonal Antibody
Gene Names
PNLIPRP1; PLRP1
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using PDGFR beta Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
PDGFR beta, Polyclonal Antibody (Cat# AAA22218)
Full Name
PDGFR beta Polyclonal Antibody
Gene Names
PDGFRB; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(SMNDC1 polyclonal antibody. Western Blot analysis of SMNDC1 expression in PC-12.)
SMNDC1, Polyclonal Antibody (Cat# AAA14799)
Full Name
SMNDC1 (Survival Motor Neuron Domain-containing Protein 1, 30 kDa Splicing Factor SMNrp, SMN-related Protein, SMNR, Survival of Motor Neuron-related-splicing Factor 30, SPF30)
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Rad21, Polyclonal Antibody (Cat# AAA28376)
Full Name
Rad21 Rabbit pAb
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
Application Data
(Dilution: WB 1:1000-2000, IHC 1:100-200)
Kv10.2, Polyclonal Antibody (Cat# AAA28905)
Full Name
Kv10.2 Polyclonal Antibody
Gene Names
KCNH5; EAG2; hEAG2; H-EAG2; Kv10.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(WB Suggested Anti-FBXW7 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysateFBXW7 is supported by BioGPS gene expression data to be expressed in Jurkat)
FBXW7, Polyclonal Antibody (Cat# AAA23527)
Full Name
FBXW7 Antibody - C-terminal region
Gene Names
FBXW7; AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (AAA19151).MAP1LC3A was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA19151) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA19151)
Full Name
Anti-MAP1LC3A Picoband antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using S100A9 Rabbit pAb.)
S100A9, Polyclonal Antibody (Cat# AAA30717)
Full Name
S100A9 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 10ug/mL Rabbit Anti-Human LEAP2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Liver Expressed Antimicrobial Peptide 2 (LEAP2), Polyclonal Antibody (Cat# AAA20082)
Full Name
Polyclonal Antibody to Liver Expressed Antimicrobial Peptide 2 (LEAP2)
Gene Names
LEAP2; LEAP-2
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IHC
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of HL-60 cells using anti-ARL6IP6 antibody (AAA19640).Overlay histogram showing HL-60 cells stained with AAA19640 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARL6IP6 Antibody (AAA19640, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ARL6IP6, Polyclonal Antibody (Cat# AAA19640)
Full Name
Anti-ARL6IP6 Antibody Picoband
Gene Names
ARL6IP6; AIP-6; PFAAP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of SiHa cells using anti-SFPQ antibody (AAA19452).Overlay histogram showing SiHa cells stained with AAA19452 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFPQ Antibody (AAA19452, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SFPQ, Polyclonal Antibody (Cat# AAA19452)
Full Name
Anti-SFPQ Antibody Picoband
Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of EIF3E using anti-EIF3E antibody (AAA19399).EIF3E was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-EIF3E Antibody (AAA19399) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
EIF3E, Polyclonal Antibody (Cat# AAA19399)
Full Name
Anti-EIF3E Antibody Picoband
Gene Names
EIF3E; INT6; EIF3S6; EIF3-P48; eIF3-p46
Reactivity
Human, Mouse
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AAA19443).c-Jun/JUN was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-c-Jun/JUN Antibody (AAA19443) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
c-Jun/JUN, Polyclonal Antibody (Cat# AAA19443)
Full Name
Anti-c-Jun/JUN Antibody Picoband
Gene Names
JUN; AP1; AP-1; c-Jun
Reactivity
Human
Applications
WB, IHC, ICC, IF
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Human uterine cancer using C12orf10 Polyclonal Antibody at dilution of 1:100 (40x lens).)
C12orf10, Polyclonal Antibody (Cat# AAA22209)
Full Name
C12orf10 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)
FKBP4, Polyclonal Antibody (Cat# AAA26868)
Full Name
FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (Biot
Gene Names
FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
Reactivity
Human, Mouse
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western Blot;Sample: Porcine Cerebrum lysatePrimary Ab: 1ug/ml Rabbit Anti-Porcine VEGFR2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Vascular Endothelial Growth Factor Receptor 2, Polyclonal Antibody (Cat# AAA20930)
Full Name
Polyclonal Antibody to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
Reactivity
Pig, Human, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
P2X3, Polyclonal Antibody (Cat# AAA29180)
Full Name
P2X3 Polyclonal Antibody
Gene Names
P2RX3; P2X3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CDK4 antibody. Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilution of 1:1000.)
CDK4, Polyclonal Antibody (Cat# AAA28411)
Full Name
[KO Validated] CDK4 Rabbit pAb
Reactivity
Human, Mouse
Applications
WB, IHC, IP, IF, ICC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-HES5 antibody (AAA19545).Overlay histogram showing PC-3 cells stained with AAA19545 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HES5 Antibody (AAA19545, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HES5, Polyclonal Antibody (Cat# AAA19545)
Full Name
Anti-HES5 Antibody Picoband
Gene Names
HES5; bHLHb38
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of PC-3 cells using anti-POC1A antibody (AAA19908).Overlay histogram showing PC-3 cells stained with AAA19908 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POC1A Antibody (AAA19908, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
POC1A, Polyclonal Antibody (Cat# AAA19908)
Full Name
Anti-POC1A Antibody Picoband
Gene Names
POC1A; PIX2; SOFT; WDR51A
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.