Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of eIF2B (Phospho-Ser2) using Mouse brain tissue lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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EIF2S2, Polyclonal Antibody (Cat# AAA31456)

Full Name

Phospho-EIF2S2 (Ser2) Antibody

Gene Names

EIF2S2; EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (83%), Rabbit (100%), Dog (83%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using MIF Polycloanl Antibody at dilution of 1:50)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using MIF Polycloanl Antibody at dilution of 1:50)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human kidney using MIF Polycloanl Antibody at dilution of 1:50)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human prostate using MIF Polycloanl Antibody at dilution of 1:50)

WB (Western Blot) (Western Blot analysis of Rat brain and Mouse brain using MIF Polyclonal Antibody at dilution of 1:500)

WB (Western Blot) (Western Blot analysis of Jurkat, Raji and 293T cells using MIF Polyclonal Antibody at dilution of 1:1000)

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MIF, Polyclonal Antibody (Cat# AAA22241)

Full Name

MIF Polyclonal Antibody

Gene Names

MIF; GIF; GLIF; MMIF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen Affinity Purification

Pricing

$200/0.025 mL $350/0.1 mL $670/2x0.1 mL $970/3x0.1 mL $1,255/4x0.1 mL $1,510/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-FER1L3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate.MYOF is strongly supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: HelaAntibody Dilution: 1.0ug/mlMYOF is strongly supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Host: RabbitTarget Name: MYOFSample Type: 721_BAntibody Dilution: 1.0ug/mlMYOF is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Host: RabbitTarget Name: CHADSample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

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FER1L3, Polyclonal Antibody (Cat# AAA23526)

Full Name

FER1L3 antibody - C-terminal region

Gene Names

MYOF; FER1L3

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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FCM (Flow Cytometry) (TMPRSS2 Antibody (Center)(AAA28646) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of TMPRSS2 antibody (Center)(AAA28646) in HepG2 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)

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TMPRSS2, Polyclonal Antibody (Cat# AAA28646)

Full Name

TMPRSS2 Antibody (Center)

Gene Names

TMPRSS2; PP9284; PRSS10

Reactivity

Reactivity: Human, Mouse
Predicted: Mouse

Applications

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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TNF-alpha, Polyclonal Antibody (Cat# AAA29158)

Full Name

TNF-alpha Polyclonal Antibody

Gene Names

TNF; DIF; TNFA; TNFSF2; TNF-alpha

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cell using BCL2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat lung using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer using BCL2 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Breast cancer using BCL2 Antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using BCL2 antibody.)

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BCL2, Polyclonal Antibody (Cat# AAA29670)

Full Name

BCL2 Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of PC-3M cells with PKA C-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining PKA C-alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PKA C-alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.)

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PKA C alpha, Polyclonal Antibody (Cat# AAA29926)

Full Name

PKA C alpha Antibody

Gene Names

PRKACA; PKACA

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis NDEL1 (Phospho-Thr219) using UV treated Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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NDEL1, Polyclonal Antibody (Cat# AAA31462)

Full Name

Phospho-NDEL1 (Thr219) Antibody

Gene Names

NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-IDH3G antibody (AAA19943).Overlay histogram showing 293T cells stained with AAA19943 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3G Antibody (AAA19943, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of IDH3G using anti-IDH3G antibody (AAA19943).IDH3G was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3G Antibody (AAA19943) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3G Antibody (AAA19943) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3G Antibody (AAA19943) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3G Antibody (AAA19943) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3G Antibody (AAA19943) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat kidney tissue lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: mouse kidney tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: rat HBZY whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3G antigen affinity purified polyclonal antibody (#AAA19943) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: human U251 whole cell lysates,Lane 6: monkey COS-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3G antigen affinity purified polyclonal antibody (#AAA19943) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDH3G at approximately 40 kDa. The expected band size for IDH3G is at 43 kDa.)

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IDH3G, Polyclonal Antibody (Cat# AAA19943)

Full Name

Anti-IDH3G Antibody Picoband

Gene Names

IDH3G; H-IDHG

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RPL19 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using RPL19 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using RPL19 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using RPL19 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain using RPL19 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using RPL19 Rabbit pAb at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RPL19 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)

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RPL19, Polyclonal Antibody (Cat# AAA28389)

Full Name

RPL19 Rabbit pAb

Gene Names

RPL19; L19

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using AAA29669 at dilution 1/40.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using AAA29669 at dilution 1/40.)

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MTMR7, Polyclonal Antibody (Cat# AAA29669)

Full Name

MTMR7 Antibody

Reactivity

Human

Applications

Immunohistochemistry (IHC)

Purity

Antigen affinity purification.

Pricing

$335/0.1 mL $640/2x0.1 mL $930/3x0.1 mL $1,200/4x0.1 mL $1,285/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using SOCS1 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SOCS1 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SOCS1 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using SOCS1 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using SOCS1 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SOCS1 antibody.)

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SOCS1, Polyclonal Antibody (Cat# AAA30693)

Full Name

SOCS1 Rabbit Polyclonal Antibody

Gene Names

SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using GAD2 Rabbit pAb (AAA10726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat brain using GAD2 Rabbit pAb (AAA10726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using GAD2 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GAD2 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GAD2 antibody (AAA10726) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GAD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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GAD2, Polyclonal Antibody (Cat# AAA10726)

Full Name

GAD2 Polyclonal Antibody

Gene Names

GAD2; GAD65

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysates,Lane 2: human SW620 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD.)

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TSPAN12, Polyclonal Antibody (Cat# AAA19174)

Full Name

Anti-TSPAN12 Picoband antibody

Gene Names

TSPAN12; EVR5; NET2; NET-2; TM4SF12

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of AMD1 using anti-AMD1 antibody (AAA19177).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMD1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AMD1 at approximately 38KD. The expected band size for AMD1 is at 38KD.)

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AMD1/Adometdc, Polyclonal Antibody (Cat# AAA19177)

Full Name

Anti-AMD1/Adometdc Antibody

Gene Names

AMD1; AMD; SAMDC; ADOMETDC

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using P4HB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using P4HB Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using P4HB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using P4HB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using P4HB Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using P4HB Polyclonal Antibody at 1:1000 dilution.)

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P4HB, Polyclonal Antibody (Cat# AAA22249)

Full Name

P4HB Polyclonal Antibody

Gene Names

P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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Kv10.2, Polyclonal Antibody (Cat# AAA28905)

Full Name

Kv10.2 Polyclonal Antibody

Gene Names

KCNH5; EAG2; hEAG2; H-EAG2; Kv10.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining PKC beta 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PKC beta 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PKC beta 2 on different lysates using anti-PKC beta 2 antibody at 1/500 dilution. Positive control�� Lane1: Rat spleen tissue Lane2: Jurkat)

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PKC beta, Polyclonal Antibody (Cat# AAA29929)

Full Name

PKC beta Antibody

Gene Names

PRKCB; PKCB; PRKCB1; PRKCB2; PKC-beta

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using RAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using RAC2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using RAC2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RAC2 Polyclonal Antibody (AAA10730) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of extracts of Mouse spleen, using RAC2 Polyclonal Antibody (AAA10730) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.)

WB (Western Blot) (Western blot analysis of extracts of HL-60 cells, using RAC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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RAC2, Polyclonal Antibody (Cat# AAA10730)

Full Name

RAC2 Polyclonal Antibody

Gene Names

RAC2; Gx; EN-7; HSPC022; p21-Rac2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresecnce (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MED4 using anti-MED4 antibody (AAA19178).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 30KD.)

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MED4, Polyclonal Antibody (Cat# AAA19178)

Full Name

Anti-MED4 Picoband Antibody

Gene Names

MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using AIF Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse skeletal muscle cells using AIF Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using AIF Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] AIF Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] AIF Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] AIF Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells using AIF Polyclonal Antibody)

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AIF, Polyclonal Antibody (Cat# AAA22250)

Full Name

AIF Polyclonal Antibody

Gene Names

AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of for immunohistochemistry.)

Application Data (All lanes: at 1:1000 dilution Lane 1: human liver lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: MOLT-4 whole cell lysates Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size : 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (039288 at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) goat anti-rabbit Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Application Data (All lanes: at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (H+L) goat anti-rabbit, Peroxidase conjugated at 1:10000 dilution. Predicted band size: 35kD Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Western Blot analysis of OPN-a/b (arrow))

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OPN-a/b, Polyclonal Antibody (Cat# AAA26858)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (Biotin)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

$1,055/0.2 mL $2,045/2x0.2 mL $2,980/3x0.2 mL $3,865/4x0.2 mL $4,515/5x0.2 mL

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MED18 using anti-MED18 antibody (AAA19179).MED18 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (AAA19179) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MED18 using anti-MED18 antibody (AAA19179).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human U2OS whole cell lysates,Lane 4: human HL-60 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED18 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED18 at approximately 24KD. The expected band size for MED18 is at 24KD.)

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MED18, Polyclonal Antibody (Cat# AAA19179)

Full Name

Anti-MED18 Picoband Antibody

Gene Names

MED18; SRB5; p28b

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEL cells using anti-LYPLA2 antibody (AAA19947).Overlay histogram showing HEL cells stained with AAA19947 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LYPLA2 Antibody (AAA19947, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of LYPLA2 using anti-LYPLA2 antibody (AAA19947).LYPLA2 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LYPLA2 Antibody (AAA19947) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LYPLA2 Antibody (AAA19947) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LYPLA2 Antibody (AAA19947) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LYPLA2 Antibody (AAA19947) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat stomach tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYPLA2 antigen affinity purified polyclonal antibody (#AAA19947) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LYPLA2 at approximately 25 kDa. The expected band size for LYPLA2 is at 25 kDa.)

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LYPLA2, Polyclonal Antibody (Cat# AAA19947)

Full Name

Anti-LYPLA2 Antibody Picoband

Gene Names

LYPLA2; APT2; APT-2; DJ886K2.4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human vermiform appendix using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using ALYREF antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using ALYREF antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ALYREF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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ALYREF, Polyclonal Antibody (Cat# AAA28139)

Full Name

ALYREF Polyclonal Antibody

Gene Names

ALYREF; ALY; BEF; REF; THOC4; ALY/REF

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Amylase, Polyclonal Antibody (Cat# AAA29163)

Full Name

Amylase Polyclonal Antibody

Gene Names

AMY1A; AMY1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining CRMP2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CRMP2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CRMP2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRMP2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CRMP2 on different lysates using anti-CRMP2 antibody at 1/5, 000 dilution. Positive control�� Lane1: Mouse brain tissue Lane2: SH-SY-5Y)

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CRMP2, Polyclonal Antibody (Cat# AAA29931)

Full Name

CRMP2 Antibody

Gene Names

DPYSL2; DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Purity

Protein affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DCK antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human vermiform appendix using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using DCK antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DCK Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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DCK, Polyclonal Antibody (Cat# AAA10732)

Full Name

DCK Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FCM (Flow Cytometry) (Flow Cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Application Data (IC staining of Hela cells)

IHC (Immunohistochemistry) (Immunohistochemical staining of formalin-fixed and paraffin-embedded human cancer tissue)

WB (Western Blot) (Western Blot analysis of Hela cell line lysate (35ug/lane) using antibody.)

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FGFR2, Polyclonal Antibody (Cat# AAA26852)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (APC)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein G Affinity Chromatography.

Pricing

$1,055/0.2 mL $2,045/2x0.2 mL $2,980/3x0.2 mL $3,865/4x0.2 mL $4,515/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using IL18 antibody at dilution of 1:50 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using IL18 antibody at dilution of 1:50 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using IL18 antibody at dilution of 1:50 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of Recombinant Human IL-18 Protei, using IL18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of Rat brain, using IL18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL18 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

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IL18, Polyclonal Antibody (Cat# AAA28388)

Full Name

IL18 Rabbit pAb

Gene Names

Il18; Igif; Il-18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with CD71 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining CD71 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD71 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD71 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD71 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.)

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CD71, Polyclonal Antibody (Cat# AAA29924)

Full Name

CD71 Antibody

Gene Names

TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR

Reactivity

Human, Mouse

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of COX IV using anti-COX IV antibody (AAA19173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.)

WB (Western Blot) (Figure 1. Western blot analysis of COX IV using anti-COX IV antibody (AAA19173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human SGC-7901 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX IV antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for COX IV at approximately 17KD. The expected band size for COX IV is at 20KD.)

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COX IV, Polyclonal Antibody (Cat# AAA19173)

Full Name

Anti-COX IV Picoband Antibody

Gene Names

COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-PYHIN1 antibody (AAA19944).Overlay histogram showing JK cells stained with AAA19944 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYHIN1 Antibody (AAA19944, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PYHIN1 using anti-PYHIN1 antibody (AAA19944).PYHIN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYHIN1 antigen affinity purified polyclonal antibody (#AAA19944) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PYHIN1 at approximately 55 kDa. The expected band size for PYHIN1 is at 55 kDa.)

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PYHIN1, Polyclonal Antibody (Cat# AAA19944)

Full Name

Anti-PYHIN1 Antibody Picoband

Gene Names

PYHIN1; IFIX

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using CORO1A Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using CORO1A Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using CORO1A Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using CORO1A Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using CORO1A Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CORO1A Polyclonal Antibody at 1:1000 dilution.)

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CORO1A, Polyclonal Antibody (Cat# AAA22248)

Full Name

CORO1A Polyclonal Antibody

Gene Names

CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FCM (Flow Cytometry) (Flow Cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)

Application Data (IC staining of Hela cells)

IHC (Immunohistochemistry) (Immunohistochemical staining of formalin-fixed and paraffin-embedded human cancer tissue)

WB (Western Blot) (Western Blot analysis of Hela cell line lysate (35ug/lane) using antibody.)

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FGFR2, Polyclonal Antibody (Cat# AAA26856)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (PE)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

FLISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Purified by Protein G Affinity Chromatography.

Pricing

$1,055/0.2 mL $2,045/2x0.2 mL $2,980/3x0.2 mL $3,865/4x0.2 mL $4,515/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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Catalase, Polyclonal Antibody (Cat# AAA29160)

Full Name

Catalase Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of PDHA1/2 (Phospho-Ser293/291) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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PDHA1/2, Polyclonal Antibody (Cat# AAA31464)

Full Name

Phospho-PDHA1/2 (Ser293/Ser291) Antibody

Gene Names

PDHA1; PDHA; PHE1A; PDHCE1A

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of rat brain cells using MAP2 Polyclonal antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain cells using MAP2 Polyclonal antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using MAP2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using MAP2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using MAP2 Polyclonal Antibody at 1:2000 dilution.)

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Map2, Polyclonal Antibody (Cat# AAA22247)

Full Name

Map2 Polyclonal Antibody

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-FBXW7 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysateFBXW7 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: GNASSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Type: 721_B Whole Cell lysatesAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Type: 721_B Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Type: 721_B Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: FBXW7Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Kidney)

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FBXW7, Polyclonal Antibody (Cat# AAA23527)

Full Name

FBXW7 Antibody - C-terminal region

Gene Names

FBXW7; AGO; CDC4; FBW6; FBW7; hAgo; FBX30; FBXW6; SEL10; hCdc4; FBXO30; SEL-10

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using SNRPE Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SNRPE Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using SNRPE Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using SNRPE Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using SNRPE Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using SNRPE Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SNRPE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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SNRPE, Polyclonal Antibody (Cat# AAA28135)

Full Name

SNRPE Polyclonal Antibody

Gene Names

SNRPE; SME; Sm-E; B-raf; HYPT11

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution or Histone H3 antibody (A15741).HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour and treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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Phospho-Histone H3-S10/T11, Polyclonal Antibody (Cat# AAA28391)

Full Name

Phospho-Histone H3-S10/T11 Rabbit pAb

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$255/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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SLC12A4, Polyclonal Antibody (Cat# AAA28903)

Full Name

SLC12A4 Polyclonal Antibody

Gene Names

SLC12A4; KCC1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-GATM Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysateGATM is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Host: RabbitTarget Name: SERPINA3Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NSUN6Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GNASSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GATMSample Tissue: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-GATM antibodyFormalin Fixed Paraffin Embedded Tissue: Human KidneyPrimary antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20xExposure Time: 0.5-2.0sec)

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GATM, Polyclonal Antibody (Cat# AAA23534)

Full Name

GATM antibody - middle region

Gene Names

GATM; AT; AGAT; CCDS3

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CUL4B antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using CUL4B antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CUL4B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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CUL4B, Polyclonal Antibody (Cat# AAA28142)

Full Name

CUL4B Polyclonal Antibody

Gene Names

CUL4B; SFM2; MRXSC; CUL-4B; MRXHF2; MRXS15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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MK, Polyclonal Antibody (Cat# AAA29166)

Full Name

MK Polyclonal Antibody

Gene Names

MDK; MK; ARAP; NEGF2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.)

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DIAPH3, Polyclonal Antibody (Cat# AAA29934)

Full Name

DIAPH3 Antibody

Gene Names

DIAPH3; AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified.

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat liver using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using MDM2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using MDM2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using MDM2 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MDM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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MDM2, Polyclonal Antibody (Cat# AAA10735)

Full Name

MDM2 Polyclonal Antibody

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-T6 Rabbit pAb (AAA28403) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-T6 Rabbit pAb (AAA28403) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Phospho-Histone H3-T6 Rabbit pAb (AAA28403) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-Histone H3-T6 antibody (AAA28403) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3-T6 antibody (AAA28403) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Phospho-Histone H3-T6 antibody (AAA28403) at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Phospho-Histone H3-T6 antibody (AAA28403) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T6 antibody (AAA28403) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37 degree C for 1 hour.HeLa cells were treated by nocodazole (50 ng/ml) at 37 degree C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)

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Histone H3-T6, Polyclonal Antibody (Cat# AAA28403)

Full Name

Phospho-Histone H3-T6 Rabbit pAb

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$255/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BMP5 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BMP5 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using BMP5 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using BMP5 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using BMP5 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using BMP5 at dilution of 1:100 (40x lens).)

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BMP5, Polyclonal Antibody (Cat# AAA30707)

Full Name

BMP5 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using FLNB antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FLNB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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FLNB, Polyclonal Antibody (Cat# AAA10740)

Full Name

FLNB Polyclonal Antibody

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.