WB (Western Blot)
(ATPIF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATPIF1 antibody detected the ATPIF1 protein (arrow).)
Rabbit anti-Human ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody
ATPIF1 Antibody (N-term)
Gene Names
ATPIF1; IP; ATPI; ATPIP
Reactivity
Human
Applications
Western Blot, ELISA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
ATPIF1; Polyclonal Antibody; ATPIF1 Antibody (N-term); ATPase inhibitor; mitochondrial; Inhibitor of F(1)F(o)-ATPase; IF(1); IF1; ATPI; anti-ATPIF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This ATPIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human ATPIF1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
8-35
Sequence Length
60
Applicable Applications for anti-ATPIF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Function
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Cellular Location
Mitochondrion.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(ATPIF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATPIF1 antibody detected the ATPIF1 protein (arrow).)
WB (Western Blot)
(ATPIF1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ATPIF1 antibody detected the ATPIF1 protein (arrow).)
Related Product Information for anti-ATPIF1 antibody
Background: This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].
Product Categories/Family for anti-ATPIF1 antibody
References
Sanchez-Cenizo, L., et al. J. Biol. Chem. 285(33):25308-25313(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Li, N., et al. Int. J. Neurosci. 120(3):217-221(2010)
Contessi, S., et al. J. Bioenerg. Biomembr. 39(4):291-300(2007)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Li, N., et al. Int. J. Neurosci. 120(3):217-221(2010)
Contessi, S., et al. J. Bioenerg. Biomembr. 39(4):291-300(2007)
Ma, J., et al. Atherosclerosis 191(1):63-72(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 12249
NCBI Official Full Name
ATPase inhibitor, mitochondrial isoform 1
NCBI Official Synonym Full Names
ATPase inhibitory factor 1
NCBI Official Symbol
ATPIF1
NCBI Official Synonym Symbols
IP; ATPI; ATPIP
NCBI Protein Information
ATPase inhibitor, mitochondrial
UniProt Protein Name
ATPase inhibitor, mitochondrial
UniProt Gene Name
ATPIF1
UniProt Synonym Gene Names
ATPI; IF(1); IF1
UniProt Entry Name
ATIF1_HUMAN
Similar Products
Product Notes
The ATPIF1 atpif1 (Catalog #AAA28683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 8-35. The ATPIF1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATPIF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ATPIF1 atpif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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