IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Hamartin (TSC1) Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human Hamartin (TSC1) Polyclonal Antibody | anti-TSC1 antibody
Hamartin (TSC1) Antibody (Center)
Gene Names
TSC1; LAM; TSC
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Hamartin (TSC1), Antibody; Hamartin (TSC1) Antibody (Center); Hamartin; Tuberous sclerosis 1 protein; TSC1; KIAA0243; TSC; anti-TSC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Hamartin (TSC1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the Central region of human Hamartin (TSC1).
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
401-430
Sequence Length
1,113
Applicable Applications for anti-TSC1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Hamartin (TSC1) Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of Hamartin (TSC1) Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(Hamartin (TSC1) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hamartin (TSC1) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Hamartin (TSC1) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hamartin (TSC1) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(TSC1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TSC1 antibody detected the TSC1 protein (arrow).)
WB (Western Blot)
(TSC1 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TSC1 antibody detected the TSC1 protein (arrow).)
Related Product Information for anti-TSC1 antibody
Implicated as a tumor suppressor. May have a function in vesicular transport. Interaction between TSC1 and TSC2 may facilitate vesicular docking.
Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). The molecular basis of TSC is a functional impairement of the hamartin-tuberin complex. TSC is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TSC is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). FCDBC is a subtype of cortical displasias linked to chronic intractable epilepsy. Cortical dysplasias display a broad spectrum of structural changes, which appear to result from changes in proliferation, migration, differentiation, and apoptosis of neuronal precursors and neurons during cortical development.
Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). The molecular basis of TSC is a functional impairement of the hamartin-tuberin complex. TSC is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. TSC is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). FCDBC is a subtype of cortical displasias linked to chronic intractable epilepsy. Cortical dysplasias display a broad spectrum of structural changes, which appear to result from changes in proliferation, migration, differentiation, and apoptosis of neuronal precursors and neurons during cortical development.
Product Categories/Family for anti-TSC1 antibody
References
Wu, J., et al., J. Cutan. Pathol. 31(5):383-387 (2004).
Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).
J, et al., J. Child Neurol. 19(2):102-106 (2004).
Murthy, V., et al., J. Biol. Chem. 279(2):1351-1358 (2004).
Astrinidis, A., et al., J. Biol. Chem. 278(51):51372-51379 (2003).
Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).
J, et al., J. Child Neurol. 19(2):102-106 (2004).
Murthy, V., et al., J. Biol. Chem. 279(2):1351-1358 (2004).
Astrinidis, A., et al., J. Biol. Chem. 278(51):51372-51379 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
129767
NCBI Official Full Name
hamartin isoform 1
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
TSC1
NCBI Official Synonym Symbols
LAM; TSC
NCBI Protein Information
hamartin
UniProt Protein Name
Hamartin
UniProt Gene Name
TSC1
UniProt Synonym Gene Names
KIAA0243; TSC
UniProt Entry Name
TSC1_HUMAN
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Product Notes
The TSC1 tsc1 (Catalog #AAA285509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 401-430. The Hamartin (TSC1) Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hamartin (TSC1) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TSC1 tsc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hamartin (TSC1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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