WB (Western Blot)
(Detection of human and mouse TSC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse TSC1 Polyclonal Antibody | anti-TSC1 antibody
Rabbit anti-TSC1 Antibody, Affinity Purified
Gene Names
TSC1; LAM; TSC
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TSC1, Antibody; Rabbit anti-TSC1 Antibody, Affinity Purified; TSC; truncated hemartin; LAM; hamartin; tuberous sclerosis 1 protein; Hamartin; TSC complex subunit 1; anti-TSC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TSC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1114 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse TSC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse TSC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse TSC1 by western blot. Samples: Whole cell lysate (10 or 50 ug) from HeLa (H) and MEF (M) cells. Antibody: Affinity purified rabbit anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for WB at 0.2 or 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.)
WB (Western Blot)
(Detection of human and mouse TSC1 by western blot. Samples: Whole cell lysate (10 or 50 ug) from HeLa (H) and MEF (M) cells. Antibody: Affinity purified rabbit anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for WB at 0.2 or 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.)
IP (Immunoprecipitation)
(Detection of human TSC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (60 ug for input and 1.5 mg for IP) from U2OS cells. Antibody: Affinity purified rabbit anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for IP at 1 ug/mg lysate. For blotting immunoprecipitated TSC1, AAA210884 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Note: TSC2 co-immunoprecipitates with TSC1. TSC2 was detected using a TSC2 antibody from another source.)
IP (Immunoprecipitation)
(Detection of human TSC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (60 ug for input and 1.5 mg for IP) from U2OS cells. Antibody: Affinity purified rabbit anti-TSC1 antibody AAA210884 (lot AAA210884-1) used for IP at 1 ug/mg lysate. For blotting immunoprecipitated TSC1, AAA210884 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Note: TSC2 co-immunoprecipitates with TSC1. TSC2 was detected using a TSC2 antibody from another source.)
Related Product Information for anti-TSC1 antibody
Tuberous sclerosis 1 protein (TSC1) forms a heterodimer with TSC2 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb.
Product Categories/Family for anti-TSC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1164
NCBI Official Full Name
hamartin isoform 1
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
TSC1
NCBI Official Synonym Symbols
LAM; TSC
NCBI Protein Information
hamartin; tumor suppressor; tuberous sclerosis 1 protein
UniProt Protein Name
Hamartin
UniProt Gene Name
TSC1
UniProt Synonym Gene Names
KIAA0243; TSC
UniProt Entry Name
TSC1_HUMAN
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Product Notes
The TSC1 tsc1 (Catalog #AAA210884) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TSC1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TSC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TSC1 tsc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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