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IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit TSC1 Polyclonal Antibody | anti-TSC1 antibody
Phospho-TSC1 (Ser505) Antibody
Gene Names
TSC1; LAM; TSC
Reactivity
Human, Mouse, RatPredicted Reactivity: Horse (100%), Sheep (100%), Dog (90%), Xenopus (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
TSC1, Antibody; Phospho-TSC1 (Ser505) Antibody; Hamartin; kiaa0243; LAM; TSC; Tsc1; Tsc1 gene; TSC1_HUMAN; Tuberous sclerosis 1; Tuberous sclerosis 1 protein; tumor suppressor; anti-TSC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (100%), Sheep (100%), Dog (90%), Xenopus (100%)
Predicted Reactivity: Horse (100%), Sheep (100%), Dog (90%), Xenopus (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-TSC1 (Ser505) Antibody detects endogenous levels of TSC1 only when phosphorylated at Ser505.
Tissue Specificity: Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Tissue Specificity: Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TSC1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TSC1 around the phosphorylation site of Ser505.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation at Ser-505 does not affect interaction with TSC2.
Subunit Structure
Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex. Interacts (via C-terminus) with the closed form of HSP90AA1 (via the middle domain and TPR repeat-binding motif). Interacts with TSC2; the interaction stabilizes TSC2 and prevents TSC2 self-aggregation. Interacts with DOCK7. Interacts with FBXW5. Interacts with TBC1D7. Interacts with WDR45B. Interacts with RPAP3 and URI1.
Similarity
The C-terminal putative coiled-coil domain is necessary for interaction with TSC2.
Subcellular Location
Cytoplasm. Membrane>Peripheral membrane protein.
Note: At steady state found in association with membranes.
Note: At steady state found in association with membranes.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HT-1080, using Phospho-TSC1(Ser505) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HT-1080, using Phospho-TSC1(Ser505) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC12(H2O2 treatment), using Phospho-TSC1 (Ser505) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from PC12(H2O2 treatment), using Phospho-TSC1 (Ser505) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TSC1 antibody
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1164
NCBI Official Full Name
Hamartin
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
TSC1
NCBI Official Synonym Symbols
LAM; TSC
NCBI Protein Information
hamartin; tumor suppressor; tuberous sclerosis 1 protein
UniProt Protein Name
Hamartin
UniProt Gene Name
TSC1
UniProt Synonym Gene Names
KIAA0243; TSC
UniProt Entry Name
TSC1_HUMAN
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Product Notes
The TSC1 tsc1 (Catalog #AAA329408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TSC1 (Ser505) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Horse (100%), Sheep (100%), Dog (90%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's TSC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TSC1 tsc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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