Rabbit Phospho-rat TSC1 (Y297) Polyclonal Antibody | anti-Tsc1 antibody

Phospho-rat TSC1 (Y297) Antibody

Cat. Num. AAA283645

• Reactivity: Rat
• Applications: ELISA, Dot Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-rat TSC1 (Y297), Antibody; Phospho-rat TSC1 (Y297) Antibody; Hamartin; Tuberous sclerosis 1 protein homolog; Tsc1; anti-Tsc1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This rat TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y297 of rat TSC1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1163

• Applicable Applications for anti-Tsc1 antibody: ELISA, DB (Dot Blot)
• Function: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed: ). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (By similarity). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (By similarity). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).
• Cellular Location: Cytoplasm {ECO:0000250|UniProtKB:Q92574}. Membrane {ECO:0000250|UniProtKB:Q92574}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q92574}. Note=At steady state found in association with membranes. {ECO:0000250|UniProtKB:Q92574}
• Tissue Location: Highly expressed in brain, spleen and kidney, followed by liver and heart
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

DB (Dot Blot)

(Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-Tsc1 antibody

In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity).

Product Categories/Family for anti-Tsc1 antibody

Phospho Antibodies

References

Inoue, H., et al. Biosci. Biotechnol. Biochem. 73(11):2488-2493(2009)
Di Nardo, A., et al. J. Neurosci. 29(18):5926-5937(2009)
Chen, P., et al. Exp. Mol. Pathol. 86(2):101-107(2009)
Momose, S., et al. Biochem. Biophys. Res. Commun. 356(3):693-698(2007)
Goncharova, E., et al. J. Cell Biol. 167(6):1171-1182(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 11177894
• NCBI GeneID: 60445
• NCBI Accession #: NP_068626.1
• NCBI GenBank Nucleotide #: NM_021854.1
• UniProt Accession #: Q9Z136
• Molecular Weight: 129022
• NCBI Official Full Name: hamartin
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: Tsc1
• NCBI Protein Information: hamartin
• UniProt Protein Name: Hamartin
• UniProt Gene Name: Tsc1
• UniProt Entry Name: TSC1_RAT