Rabbit TSC1 Polyclonal Antibody | anti-TSC1 antibody

TSC1 Antibody

Cat. Num. AAA40202

• Gene Names: TSC1; LAM; TSC
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: TSC1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TSC1, Antibody; TSC1 Antibody; TSC1; LAM; TSC; KIAA0243; Hamartin; Tuberous sclerosis 1 protein; tuberous sclerosis 1; anti-TSC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TSC1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1164

• Applicable Applications for anti-TSC1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: TSC1 antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. TSC1 antibody will recognize both isoforms of TSC1.
• Buffer: TSC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TSC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 μg/mL)

WB (Western Blot)

(Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.)

Related Product Information for anti-TSC1 antibody

TSC1 Antibody: Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

NCBI and Uniprot Product Information

• NCBI GI #: 2331281
• NCBI GeneID: 7248
• UniProt Accession #: Q92574; Q5VVN5; B7Z897
• Molecular Weight: 124,015 Da
• NCBI Official Full Name: hamartin
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC
• NCBI Protein Information: hamartin; tumor suppressor; tuberous sclerosis 1 protein
• UniProt Protein Name: Hamartin
• UniProt Gene Name: TSC1
• UniProt Synonym Gene Names: KIAA0243; TSC
• UniProt Entry Name: TSC1_HUMAN