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WB (Western Blot)
(Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody BL1350 (Cat. No. AAA210856) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using BL1350, BL1351 (Cat. No. or a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using a mixture of BL1350 and BL1351 (each at 1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).)
Rabbit anti-Human, Mouse PCNA Polyclonal Antibody | anti-PCNA antibody
Rabbit anti-PCNA Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PCNA, Antibody; Rabbit anti-PCNA Antibody, Affinity Purified; proliferating cell nuclear antigen; PCNA; DNA polymerase delta auxiliary protein; ATLD2; cyclin; Proliferating cell nuclear antigen; anti-PCNA antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-PCNA antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Chicken, Bovine
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody BL1350 (Cat. No. AAA210856) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using BL1350, BL1351 (Cat. No. or a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using a mixture of BL1350 and BL1351 (each at 1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).)
WB (Western Blot)
(Detection of human PCNA by western blot and immunoprecipitation. Samples: A. Whole cell lysate (WCL) from A-431 cells. B. WCL (500 ug for IP; 30 ug for WCL lane) from MDA-MB-468 cells. Antibodies: A. Affinity purified rabbit anti-PCNA antibody BL1350 (Cat. No. AAA210856) used at the indicated concentrations for WB. B. PCNA was immunoprecipitated using BL1350, BL1351 (Cat. No. or a mouse monoclonal antibody to PCNA (mAb). Control mock IP was performed using normal mouse IgG (M-IgG) and normal rabbit IgG (R-IgG). Immunoprecipitated PCNA was blotted using a mixture of BL1350 and BL1351 (each at 1 ug/ml). Detection: Chemiluminescence with an exposure time of 2 minutes (A) or 5 seconds (B).)
WB (Western Blot)
(Detection of human and mouse PCNA by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody AAA210856 (lot AAA210856-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse PCNA by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody AAA210856 (lot AAA210856-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA210856 Lot1) used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA (Cat. No. AAA210856 Lot1) used at a dilution of 1:10,000 (0.1ug/ml). Detection: DAB)
FCM/FACS (Flow Cytometry)
(Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X10^6 cells were stained with 0.03 ug anti-PCNA [AAA210856]. Secondary detection was performed with FITC conjugated goat F(ab')2 anti-rabbit antibody, and DNA stained with PI.)
FCM/FACS (Flow Cytometry)
(Detection of PCNA Versus DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. 1 X10^6 cells were stained with 0.03 ug anti-PCNA [AAA210856]. Secondary detection was performed with FITC conjugated goat F(ab')2 anti-rabbit antibody, and DNA stained with PI.)
Related Product Information for anti-PCNA antibody
PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.
Product Categories/Family for anti-PCNA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,769 Da
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Protein Information
proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
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Product Notes
The PCNA pcna (Catalog #AAA210856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PCNA Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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