WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution Lane 1:Hela whole cell lysate Lane 2:A549 whole cell lysate Lane 3:A431 whole cell lysate Lane 4:MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human PD L1 Polyclonal Antibody | anti-PDL1 antibody
PD L1 Antibody (C-Term)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PD L1, Antibody; PD L1 Antibody (C-Term); Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; B7 homolog 1; B7-H1; CD274; anti-PDL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Clone Number
R03065
Specificity
This PD L1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human PD L1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applicable Applications for anti-PDL1 antibody
WB (Western Blot)
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution Lane 1:Hela whole cell lysate Lane 2:A549 whole cell lysate Lane 3:A431 whole cell lysate Lane 4:MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution Lane 1:Hela whole cell lysate Lane 2:A549 whole cell lysate Lane 3:A431 whole cell lysate Lane 4:MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution Lane 1:Hela whole cell lysate Lane 2:A549 whole cell lysate Lane 3:A431 whole cell lysate Lane 4:MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution Lane 1:Hela whole cell lysate Lane 2:A549 whole cell lysate Lane 3:A431 whole cell lysate Lane 4:MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-PD L1 Antibody (C-Term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PDL1 antibody
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed: 11015443, PubMed: 28813417, PubMed: 28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed: 11015443, PubMed: 28813417, PubMed: 28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10)(PubMed: 10581077).
Product Categories/Family for anti-PDL1 antibody
References
Dong H.,et al.Nat. Med. 5:1365-1369(1999).Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005).Chi X.-Y.,et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.Ota T.,et al.Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The PDL1 cd274 (Catalog #AAA296705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PD L1 Antibody (C-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD L1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PDL1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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