IP (Immunoprecipitation)
(SIRT1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-SIRT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SIRT1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size: 130 kDa)
Rabbit anti-Human SIRT1 Polyclonal Antibody | anti-SIRT1 antibody
Anti-SIRT1 Antibody, Rabbit Polyclonal
Gene Names
SIRT1; SIR2L1
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SIRT1, Antibody; Anti-SIRT1 Antibody, Rabbit Polyclonal; SIRT1 Antibody, Rabbit PAb, Antigen Affinity Purified; sirtuin 1; Anti-SIR2L1 Antibody; anti-SIRT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SIRT1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SIRT1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human SIRT1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human SIRT1 (rh SIRT1). SIRT1 specific IgG was purified by Human SIRT1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SIRT1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-SIRT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SIRT1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size: 130 kDa)
IP (Immunoprecipitation)
(SIRT1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-SIRT1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SIRT1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size: 130 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of SIRT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SIRT1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of SIRT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SIRT1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)
WB (Western Blot)
(Anti-SIRT1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:130 kDa)
WB (Western Blot)
(Anti-SIRT1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:130 kDa)
Related Product Information for anti-SIRT1 antibody
SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1-and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.
Product Categories/Family for anti-SIRT1 antibody
References
Sharma A, et al. (2012) Interactomic and pharmacological insights on human Sirt-1. Front Pharmacol. 3-40.Sun C, et al. (2007) SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 6(4):307-19.Rodgers JT, et al. (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 434(7029):113-8.Nemoto S, et al. (2005) SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. J Biol Chem. 280(16):16456-60.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,066 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform a
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2L1
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1
UniProt Entry Name
SIR1_HUMAN
Similar Products
Product Notes
The SIRT1 sirt1 (Catalog #AAA255466) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.