Rabbit anti-Human SIRT1 Polyclonal Antibody | anti-SIRT1 antibody

Anti-SIRT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA254347

• Gene Names: SIRT1; SIR2L1
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: SIRT1, Antibody; Anti-SIRT1 Antibody, Rabbit Polyclonal; SIRT1 Antibody, Rabbit PAb, Antigen Affinity Purified; sirtuin 1; Anti-SIR2L1 Antibody; anti-SIRT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SIRT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SIRT1 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human SIRT1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SIRT1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-SIRT1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:81 kDaObserved band size:135 kDa)

IP (Immunoprecipitation)

(SIRT1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate0.5 uL anti-SIRT1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-SIRT1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)

Related Product Information for anti-SIRT1 antibody

SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1-and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus.

Product Categories/Family for anti-SIRT1 antibody

p53 Pathway

References

Sharma A, et al. (2012) Interactomic and pharmacological insights on human Sirt-1. Front Pharmacol. 3-40.Sun C, et al. (2007) SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 6(4):307-19.Rodgers JT, et al. (2005) Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1. Nature. 434(7029):113-8.Nemoto S, et al. (2005) SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. J Biol Chem. 280(16):16456-60.

NCBI and Uniprot Product Information

• NCBI GI #: 215982798
• NCBI GeneID: 23411
• NCBI Accession #: NP_001135970.1
• NCBI GenBank Nucleotide #: NM_001142498.1
• UniProt Accession #: Q96EB6; Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0
• Molecular Weight: 81,681 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-1 isoform b
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: SIRT1
• NCBI Official Synonym Symbols: SIR2L1
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-1
• UniProt Gene Name: SIRT1
• UniProt Synonym Gene Names: SIR2L1; hSIRT1; hSIR2; 75SirT1
• UniProt Entry Name: SIR1_HUMAN