Human protein arginine methyltransferase 5 ELISA Kit | PRMT5 elisa kit

Human Protein arginine N-methyltransferase 5, PRMT5 ELISA Kit

Cat. Num. AAA299418

• Gene Names: PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• Reactivity: Human
• Synonyms: protein arginine methyltransferase 5; N/A; Human Protein arginine N-methyltransferase 5, PRMT5 ELISA Kit; Human Protein arginine N-methyltransferase 5 (PRMT5) ELISA kit; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; PRMT5 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human PRMT5 and analogues was observed.
• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.

• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PRMT5 concentrations in Human serum, plasma and other body fluids. Using Purified Human PRMT5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PRMT5 and PRMT5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PRMT5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PRMT5 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PRMT5 concentrations in Human serum, plasma and other body fluids. Using Purified Human PRMT5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PRMT5 and PRMT5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PRMT5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 88900507
• NCBI GeneID: 10419
• NCBI Accession #: NP_001034708.1
• NCBI GenBank Nucleotide #: NM_001039619.2
• UniProt Accession #: O14744; Q6IBR1; Q9UKH1; A8MTP3; A8MZ91; B4DX49; B4DY30; B5BU10; D3DS33; E2QRE7
• Molecular Weight: 72,684 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 5 isoform b
• NCBI Official Synonym Full Names: protein arginine methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
• UniProt Entry Name: ANM5_HUMAN