Search results for "Recombinant Proteins"

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using UMPS antibody.)

UMPS, Polyclonal Antibody (Cat# AAA28172)

Full Name

UMPS Polyclonal Antibody

Gene Names

UMPS; OPRT

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)

PHB, Monoclonal Antibody (Cat# AAA25791)

Full Name

PHB (Prohibitin) (PE)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

GROS1, Monoclonal Antibody (Cat# AAA24819)

Full Name

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (Biotin)

Gene Names

P3H1; OI8; GROS1; LEPRE1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

Rad21, Polyclonal Antibody (Cat# AAA28376)

Full Name

Rad21 Rabbit pAb

Gene Names

RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).)

Vimentin, Antibody (Cat# AAA23937)

Full Name

Vimentin (Mesenchymal Cell Marker)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Hela cells using anti- ETF/TEAD2 antibody (AAA19325).Overlay histogram showing Hela cells stained withAAA19325 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ETF/TEAD2 Antibody (AAA19325, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ETF/TEAD2, Polyclonal Antibody (Cat# AAA19325)

Full Name

Anti-ETF/TEAD2 Antibody

Gene Names

TEAD2; ETF; TEF4; TEF-4; TEAD-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (AAA19328).Overlay histogram showing U937 cells stained with AAA19328 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (AAA19328,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

BDH1, Polyclonal Antibody (Cat# AAA19328)

Full Name

Anti-BDH1 Antibody

Gene Names

BDH1; BDH; SDR9C1

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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eRF1/ETF1, Monoclonal Antibody (Cat# AAA19382)

Full Name

Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)

Gene Names

ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PTPIP51/RMDN3 antibody (AAA19923).Overlay histogram showing HepG2 cells stained with AAA19923 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (AAA19923).PTPIP51/RMDN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPIP51/RMDN3 antigen affinity purified polyclonal antibody (#AAA19923) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTPIP51/RMDN3 at approximately 55 kDa. The expected band size for PTPIP51/RMDN3 is at 52 kDa.)

PTPIP51/RMDN3, Polyclonal Antibody (Cat# AAA19923)

Full Name

Anti-PTPIP51/RMDN3 Antibody Picoband

Gene Names

RMDN3; RMD3; RMD-3; FAM82C; FAM82A2; ptpip51

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HL-60 cells using anti-RPL32 antibody (AAA19570).Overlay histogram showing HL-60 cells stained with AAA19570 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL32 Antibody (AAA19570, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RPL32, Polyclonal Antibody (Cat# AAA19570)

Full Name

Anti-RPL32 Antibody Picoband

Gene Names

RPL32; L32; PP9932

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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BCKDK, Polyclonal Antibody (Cat# AAA19589)

Full Name

Anti-BCKDK Antibody Picoband

Gene Names

BCKDK; BDK; BCKDKD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of CTNS expression in PC-12)

Cystinosin, Monoclonal Antibody (Cat# AAA25071)

Full Name

Cystinosin (CTNS) (FITC)

Gene Names

CTNS; PQLC4; SLC66A4; CTNS-LSB

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-RGS6 antibody (AAA19308).Overlay histogram showing MCF-7 cells stained with AAA19308 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS6 Antibody (AAA19308, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RGS6, Polyclonal Antibody (Cat# AAA19308)

Full Name

Anti-RGS6 Antibody

Gene Names

RGS6; GAP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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LSM5, Polyclonal Antibody (Cat# AAA19332)

Full Name

Anti-LSM5 Antibody

Gene Names

LSM5; YER146W

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-NDUFB5 antibody (AAA19333).Overlay histogram showing A431 cells stained with AAA19333 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB5 Antibody (AAA19333, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NDUFB5, Polyclonal Antibody (Cat# AAA19333)

Full Name

Anti-NDUFB5 Antibody

Gene Names

NDUFB5; SGDH; CISGDH

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (AAA19311).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey kidney tissue lysatesLane 5: human SW620 whole cell lysatesLane 6: human Raji whole cell lysatesLane 7: rat kidney tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # AAA19311) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.)

CISD2, Polyclonal Antibody (Cat# AAA19311)

Full Name

Anti-CISD2 Antibody

Gene Names

CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-QARS1 antibody (AAA19879).Overlay histogram showing THP-1 cells stained with AAA19879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QARS1 Antibody (AAA19879, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of QARS1 using anti-QARS1 antibody (AAA19879).QARS1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat RH35 whole cell lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QARS1 antigen affinity purified polyclonal antibody (#AAA19879) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for QARS1 at approximately 88 kDa. The expected band size for QARS1 is at 88 kDa.)

QARS1, Polyclonal Antibody (Cat# AAA19879)

Full Name

Anti-QARS1 Antibody Picoband

Gene Names

QARS; GLNRS; MSCCA; PRO2195

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Jurkat cells using anti-Calmegin/CLGN antibody (AAA19539).Overlay histogram showing Jurkat cells stained with AAA19539 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calmegin/CLGN Antibody (AAA19539, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Calmegin/CLGN, Polyclonal Antibody (Cat# AAA19539)

Full Name

Anti-Calmegin/CLGN Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Epac1/RAPGEF3 antibody (AAA19459).Overlay histogram showing MCF-7 cells stained with AAA19459 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Epac1/RAPGEF3, Polyclonal Antibody (Cat# AAA19459)

Full Name

Anti-Epac1/RAPGEF3 Antibody Picoband

Gene Names

RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI

Reactivity

Human, Mouse, Monkey

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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KIFC1, Polyclonal Antibody (Cat# AAA19543)

Full Name

Anti-KIFC1 Antibody Picoband

Gene Names

KIFC1; HSET; KNSL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

RAB7B, Monoclonal Antibody (Cat# AAA25221)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7) (FITC)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of OPA1 expression in transfected 293T cell line by OPA1 monoclonal antibody (M06), clone 8A32.Lane 1: OPA1 transfected lysate (Predicted MW: 111.6 KDa).Lane 2: Non-transfected lysate.)

OPA1, Monoclonal Antibody (Cat# AAA26153)

Full Name

OPA1 (Pptic Atrophy 1 (Autosomal Dominant), FLJ12460, KIAA0567, MGM1, NPG, NTG, LargeG) (APC)

Gene Names

OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14

Applications

Western Blot

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged RPL36A is ~1ng/ml as a capture antibody.)

RPL36A, Monoclonal Antibody (Cat# AAA25230)

Full Name

RPL36A (60S Ribosomal Protein L36a, 60S Ribosomal Protein L44, Cell Migration-inducing Gene 6 Protein, Cell Growth-inhibiting Gene 15 Protein, RPL44, GIG15, MIG6, MGC72020) (FITC)

Gene Names

RPL36A; L36A; L44L; MIG6; RPL44

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using JMJD6 Antibody at dilution of 1:100 (40x lens).)

JMJD6, Polyclonal Antibody (Cat# AAA28145)

Full Name

JMJD6 Polyclonal Antibody

Gene Names

JMJD6; PSR; PTDSR; PTDSR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)

S100A6, Monoclonal Antibody (Cat# AAA25533)

Full Name

S100A6 (Protein S100-A6, S100 Calcium-binding Protein A6, Calcyclin, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) (HRP)

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

EFHD1, Monoclonal Antibody (Cat# AAA24196)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (AP)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

CSTF3, Monoclonal Antibody (Cat# AAA24771)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (Biotin)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using BTRC antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

BTRC, Polyclonal Antibody (Cat# AAA28368)

Full Name

BTRC Rabbit pAb

Gene Names

BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)

GOT1, Monoclonal Antibody (Cat# AAA11733)

Full Name

GOT1 antibody

Gene Names

GOT1; AST1; cCAT; GIG18; cAspAT; ASTQTL1

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence

Purity

By protein-A affinity chromatography

Pricing

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WB (Western Blot) (Western Blot Analysis of Panc-1 cell lysate using S100A4 Recombinant Mouse Monoclonal Antibody (rS100A4/1481).)

S100A4/Metastasin/Calvasculin, Antibody (Cat# AAA23934)

Full Name

S100A4/Metastasin/Calvasculin (Marker of Tumor Metastasis)

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED4, Polyclonal Antibody (Cat# AAA19178)

Full Name

Anti-MED4 Picoband Antibody

Gene Names

MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-OTULIN antibody (AAA19319).Overlay histogram showing 293T cells stained with AAA19319 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTULIN Antibody (AAA19319, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

OTULIN, Polyclonal Antibody (Cat# AAA19319)

Full Name

Anti-OTULIN Antibody

Gene Names

OTULIN; GUM; FAM105B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-Hamartin/TSC1 antibody (AAA19734).Overlay histogram showing THP-1 cells stained with AAA19734 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hamartin/TSC1 Antibody (AAA19734, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (AAA19734).Hamartin/TSC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin/TSC1 antigen affinity purified polyclonal antibody (#AAA19734) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Hamartin/TSC1 at approximately 160 kDa. The expected band size for Hamartin/TSC1 is at 130 kDa.)

Hamartin/TSC1, Polyclonal Antibody (Cat# AAA19734)

Full Name

Anti-Hamartin/TSC1 Antibody Picoband

Gene Names

TSC1; LAM; TSC

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FEN1, Monoclonal Antibody (Cat# AAA19686)

Full Name

Anti-FEN1 Antibody Picoband (monoclonal, 6F3F2)

Gene Names

FEN1; MF1; RAD2; FEN-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS7 is approximately 1ng/ml as a capture antibody.)

RPS7, Monoclonal Antibody (Cat# AAA14787)

Full Name

RPS7 (40S Ribosomal Protein S7)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E1 (Green) and non-stained ES-2 cells (Black) as negative control.)

STIP1, Monoclonal Antibody (Cat# AAA26540)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (HRP)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

SNX1, Monoclonal Antibody (Cat# AAA25552)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (HRP)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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CDC45L, Monoclonal Antibody (Cat# AAA19360)

Full Name

Anti-CDC45L Antibody (monoclonal, 6H6)

Gene Names

CDC45; CDC45L; CDC45L2; PORC-PI-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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GRX2/GLRX2, Polyclonal Antibody (Cat# AAA19305)

Full Name

Anti-GRX2/GLRX2 Antibody

Gene Names

GLRX2; GRX2; CGI-133

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MAP4K3 antibody (AAA19882).Overlay histogram showing 293T cells stained with AAA19882 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP4K3 Antibody (AAA19882, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MAP4K3 using anti-MAP4K3 antibody (AAA19882).MAP4K3 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP4K3 Antibody (AAA19882) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP4K3 Antibody (AAA19882) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP4K3 Antibody (AAA19882) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP4K3 Antibody (AAA19882) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K3 antigen affinity purified polyclonal antibody (#AAA19882) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP4K3 at approximately 100 kDa. The expected band size for MAP4K3 is at 101 kDa.)

MAP4K3, Polyclonal Antibody (Cat# AAA19882)

Full Name

Anti-MAP4K3 Antibody Picoband

Gene Names

MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-BCAT2 antibody (AAA19868).Overlay histogram showing MCF-7 cells stained with AAA19868 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT2 Antibody (AAA19868, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of BCAT2 using anti-BCAT2 antibody (AAA19868).BCAT2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCAT2 Antibody (AAA19868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (#AAA19868) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 38-42 kDa.)

BCAT2, Polyclonal Antibody (Cat# AAA19868)

Full Name

Anti-BCAT2 Antibody Picoband

Gene Names

BCAT2; BCAM; BCT2; PP18; BCATM

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PCMT1 antibody (AAA19819).Overlay histogram showing K562 cells stained with AAA19819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCMT1 Antibody (AAA19819, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PCMT1 using anti-PCMT1 antibody (AAA19819).PCMT1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (#AAA19819) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 25-28 kDa.)

PCMT1, Polyclonal Antibody (Cat# AAA19819)

Full Name

Anti-PCMT1 Antibody Picoband

Gene Names

PCMT1; PIMT

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Caco-2 cells using anti-ICAM1 antibody (AAA19659).Overlay histogram showing Caco-2 cells stained with AAA19659 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ICAM1 Antibody (AAA19659, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ICAM1, Monoclonal Antibody (Cat# AAA19659)

Full Name

Anti-ICAM1 Antibody Picoband (monoclonal, 6F2C3)

Gene Names

ICAM1; BB2; CD54; P3.58

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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CPSF6, Monoclonal Antibody (Cat# AAA19714)

Full Name

Anti-CPSF6 Antibody Picoband (monoclonal, 3F11E1)

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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EXOSC8, Polyclonal Antibody (Cat# AAA19607)

Full Name

Anti-EXOSC8 Antibody Picoband

Gene Names

EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IRBIT/AHCYL1, Polyclonal Antibody (Cat# AAA19604)

Full Name

Anti-IRBIT/AHCYL1 Antibody Picoband

Gene Names

AHCYL1; DCAL; IRBIT; PPP1R78; PRO0233; XPVKONA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot analysis of OPA1 expression in transfected 293T cell line by OPA1 monoclonal antibody (M06), clone 8A32.Lane 1: OPA1 transfected lysate (Predicted MW: 111.6 KDa).Lane 2: Non-transfected lysate.)

OPA1, Monoclonal Antibody (Cat# AAA26286)

Full Name

OPA1 (Pptic Atrophy 1 (Autosomal Dominant), FLJ12460, KIAA0567, MGM1, NPG, NTG, LargeG) (Biotin)

Gene Names

OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14

Applications

Western Blot

Purity

Purified

Pricing

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Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using TACSTD2 Mouse Monoclonal Antibody (TACSTD2/2153) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)