Search results for "Recombinant Proteins"

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PTRF antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

PTRF, Polyclonal Antibody (Cat# AAA28370)

Full Name

PTRF Rabbit pAb

Gene Names

PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)

STIP1, Monoclonal Antibody (Cat# AAA26316)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)

LDB3, Monoclonal Antibody (Cat# AAA25733)

Full Name

LDB3 (LIM Domain-binding Protein 3, Protein Cypher, Z-band Alternatively Spliced PDZ-motif Protein, KIAA0613, ZASP) (PE)

Gene Names

LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.)

Ubc9, Monoclonal Antibody (Cat# AAA14109)

Full Name

Anti-Ubc9 Mouse mAb

Gene Names

UBE2I; P18; UBC9; C358B7.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of K562 cells using anti-PDIA6 antibody (AAA19282).Overlay histogram showing K562 cells stained with AAA19282 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIA6 Antibody (AAA19282, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PDIA6, Polyclonal Antibody (Cat# AAA19282)

Full Name

Anti-PDIA6 Antibody

Gene Names

PDIA6; P5; ERP5; TXNDC7

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of PC-3 cells using anti-HIST1/IST1 antibody (AAA19848).Overlay histogram showing PC-3 cells stained with AAA19848 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIST1/IST1 Antibody (AAA19848, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (AAA19848).HIST1/IST1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HIST1/IST1 Antibody (AAA19848) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human MRC-5 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse lung tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (#AAA19848) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.)

hIST1/IST1, Polyclonal Antibody (Cat# AAA19848)

Full Name

Anti-hIST1/IST1 Antibody Picoband

Gene Names

IST1; OLC1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U251 cells using anti-NT5C antibody (AAA19949).Overlay histogram showing U251 cells stained with AAA19949 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NT5C Antibody (AAA19949, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NT5C using anti-NT5C antibody (AAA19949).NT5C was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NT5C Antibody (AAA19949) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NT5C Antibody (AAA19949) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NT5C Antibody (AAA19949) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NT5C Antibody (AAA19949) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U251 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HL-60 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human A549 whole cell lysates,Lane 7: human U20S whole cell lysates,Lane 8: human SH-SY5Y whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT5C antigen affinity purified polyclonal antibody (#AAA19949) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NT5C at approximately 24 kDa. The expected band size for NT5C is at 24 kDa.)

NT5C, Polyclonal Antibody (Cat# AAA19949)

Full Name

Anti-NT5C Antibody Picoband

Gene Names

NT5C; DNT; cdN; DNT1; P5N2; PN-I; HEL74; PN-II; UMPH2; dNT-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MARK3 antibody (AAA19841).Overlay histogram showing 293T cells stained with AAA19841 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MARK3 Antibody (AAA19841, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MARK3 using anti-MARK3 antibody (AAA19841).MARK3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MARK3 Antibody (AAA19841) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MARK3 Antibody (AAA19841) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MARK3 Antibody (AAA19841) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MARK3 Antibody (AAA19841) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: rat NRK whole cell lysates,Lane 4: mouse EL-4 whole cell lysates,Lane 5: mouse 4T1 whole cell lysates,Lane 6: mouse J774A.1 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARK3 antigen affinity purified polyclonal antibody (#AAA19841) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MARK3 at approximately 80 kDa. The expected band size for MARK3 is at 80 kDa.)

MARK3, Polyclonal Antibody (Cat# AAA19841)

Full Name

Anti-MARK3 Antibody Picoband

Gene Names

MARK3; KP78; CTAK1; PAR1A; Par-1a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-HES5 antibody (AAA19545).Overlay histogram showing PC-3 cells stained with AAA19545 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HES5 Antibody (AAA19545, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HES5, Polyclonal Antibody (Cat# AAA19545)

Full Name

Anti-HES5 Antibody Picoband

Gene Names

HES5; bHLHb38

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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NDUFS2, Polyclonal Antibody (Cat# AAA19555)

Full Name

Anti-NDUFS2 Antibody Picoband

Gene Names

NDUFS2; CI-49

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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TRMT10C, Polyclonal Antibody (Cat# AAA19610)

Full Name

Anti-TRMT10C Antibody Picoband

Gene Names

TRMT10C; HNYA; MRPP1; RG9MTD1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA25525)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (HRP)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA24935)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (Biotin)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7.Lane 1: SMAD3 transfected lysate (48 KDa).Lane 2: Non-transfected lysate.)

SMAD3, Monoclonal Antibody (Cat# AAA26515)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (HRP)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

RBM9, Monoclonal Antibody (Cat# AAA25815)

Full Name

RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA) (PE)

Gene Names

RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

METTL1, Polyclonal Antibody (Cat# AAA19336)

Full Name

Anti-METTL1 Antibody

Gene Names

METTL1; TRM8; TRMT8; C12orf1; YDL201w

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of Daudi cells using anti-RBM15B antibody (AAA19962).Overlay histogram showing Daudi cells stained with AAA19962 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15B Antibody (AAA19962, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of RBM15B using anti-RBM15B antibody (AAA19962).RBM15B was detected in a paraffin-embedded section of human undifferentiated carcinoma of pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15B Antibody (AAA19962) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat C6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15B antigen affinity purified polyclonal antibody (#AAA19962) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBM15B at approximately 110 kDa. The expected band size for RBM15B is at 97 kDa.)

RBM15B, Polyclonal Antibody (Cat# AAA19962)

Full Name

Anti-RBM15B Antibody Picoband

Gene Names

RBM15B; OTT3; HUMAGCGB

Reactivity

Human, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Giantin/GOLGB1, Polyclonal Antibody (Cat# AAA19596)

Full Name

Anti-Giantin/GOLGB1 Antibody Picoband

Gene Names

GOLGB1; GCP; GCP372; GOLIM1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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SND1, Monoclonal Antibody (Cat# AAA19700)

Full Name

Anti-SND1 Antibody Picoband (monoclonal, 6G3B4)

Gene Names

SND1; p100; TDRD11

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ANXA8L2 antibody. Blue: DAPI for nuclear staining.)

ANXA8L1, Polyclonal Antibody (Cat# AAA28204)

Full Name

ANXA8L1 Polyclonal Antibody

Gene Names

ANXA8; ANX8; CH17-360D5.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)

GLMN, Monoclonal Antibody (Cat# AAA24816)

Full Name

GLMN (FAP48, FAP68, VMGLOM, Glomulin, FK506-binding Protein-associated Protein, FAP, FKBP-associated Protein) (Biotin)

Gene Names

GLMN; FAP; GVM; GLML; FAP48; FAP68; FKBPAP; VMGLOM

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using COPB2 antibody at dilution of 1:100 (40x lens).)

COPB2, Polyclonal Antibody (Cat# AAA28102)

Full Name

COPB2 Polyclonal Antibody

Gene Names

COPB2; beta'-COP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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HIRIP3, Polyclonal Antibody (Cat# AAA28166)

Full Name

HIRIP3 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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RPS6KA3, Polyclonal Antibody (Cat# AAA28081)

Full Name

RPS6KA3 Polyclonal Antibody

Gene Names

RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HELA cells using anti-DNAJC10 antibody (AAA19320).Overlay histogram showing HELA cells stained with AAA19320 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJC10 Antibody (AAA19320, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DNAJC10, Polyclonal Antibody (Cat# AAA19320)

Full Name

Anti-DNAJC10 Antibody

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

STIP1, Monoclonal Antibody (Cat# AAA25855)

Full Name

STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (PE)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using HSP90AB1 antibody. Blue: DAPI for nuclear staining.)

Hsp90beta, Polyclonal Antibody (Cat# AAA28434)

Full Name

Hsp90beta Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of RFX1 using anti-RFX1 antibody (AAA19814).RFX1 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RFX1 using anti-RFX1 antibody (AAA19814).RFX1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX1 Antibody (AAA19814) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX1 antigen affinity purified polyclonal antibody (#AAA19814) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFX1 at approximately 135 kDa. The expected band size for RFX1 is at 105 kDa.)

RFX1, Polyclonal Antibody (Cat# AAA19814)

Full Name

Anti-RFX1 Antibody Picoband

Gene Names

RFX1; EFC; RFX

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U937 cells using anti-NDUFAF4 antibody (AAA19964).Overlay histogram showing U937 cells stained with AAA19964 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFAF4 Antibody (AAA19964, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of NDUFAF4 using anti-NDUFAF4 antibody (AAA19964).NDUFAF4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFAF4 Antibody (AAA19964) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFAF4 Antibody (AAA19964) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFAF4 Antibody (AAA19964) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFAF4 Antibody (AAA19964) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MDA-MB-453 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human Ramos whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAF4 antigen affinity purified polyclonal antibody (#AAA19964) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (21,25 kDa.)

NDUFAF4, Polyclonal Antibody (Cat# AAA19964)

Full Name

Anti-NDUFAF4 Antibody Picoband

Gene Names

NDUFAF4; My013; C6orf66; HRPAP20; HSPC125; bA22L21.1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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SRP72, Polyclonal Antibody (Cat# AAA19559)

Full Name

Anti-SRP72 Antibody Picoband

Gene Names

SRP72; BMFF; BMFS1; HEL103

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using UHRF1 antibody. Blue: DAPI for nuclear staining.)

UHRF1, Polyclonal Antibody (Cat# AAA10713)

Full Name

UHRF1 Polyclonal Antibody

Gene Names

UHRF1; Np95; hNP95; ICBP90; RNF106; hUHRF1; huNp95

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of TRIM6 using anti-TRIM6 antibody.)

TRIM6, Polyclonal Antibody (Cat# AAA19195)

Full Name

Anti-TRIM6 Antibody

Gene Names

TRIM6; RNF89

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of SH-SY5Y cells using anti-IPO9 antibody (AAA19940).Overlay histogram showing SH-SY5Y cells stained with AAA19940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IPO9 Antibody (AAA19940, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of IPO9 using anti-IPO9 antibody (AAA19940).IPO9 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IPO9 Antibody (AAA19940) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IPO9 antigen affinity purified polyclonal antibody (#AAA19940) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IPO9 at approximately 120 kDa. The expected band size for IPO9 is at 120 kDa.)

IPO9, Polyclonal Antibody (Cat# AAA19940)

Full Name

Anti-IPO9 Antibody Picoband

Gene Names

IPO9; Imp9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SMIM8 using anti-SMIM8 antibody (AAA20009).SMIM8 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SMIM8 Antibody (AAA20009) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

SMIM8, Polyclonal Antibody (Cat# AAA20009)

Full Name

Anti-SMIM8 Antibody

Gene Names

SMIM8; C6orf162; dJ102H19.2

Reactivity

Human

Applications

Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MED26 antibody (AAA19925).Overlay histogram showing 293T cells stained with AAA19925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MED26 Antibody (AAA19925, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MED26 using anti-MED26 antibody (AAA19925) and anti-Beta Tubulin antibody (M01857-3).MED26 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MED26 Antibody (AAA19925) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MED26 Antibody (AAA19925) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MED26 Antibody (AAA19925) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED26 antigen affinity purified polyclonal antibody (#AAA19925) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MED26 at approximately 65 kDa. The expected band size for MED26 is at 65 kDa.)

MED26, Polyclonal Antibody (Cat# AAA19925)

Full Name

Anti-MED26 Antibody Picoband

Gene Names

MED26; CRSP7; CRSP70

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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TRMT6, Polyclonal Antibody (Cat# AAA19634)

Full Name

Anti-TRMT6 Antibody Picoband

Gene Names

TRMT6; GCD10; CGI-09; Gcd10p

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using STRN3 antibody. Blue: DAPI for nuclear staining.)

STRN3, Polyclonal Antibody (Cat# AAA28082)

Full Name

STRN3 Polyclonal Antibody

Gene Names

STRN3; SG2NA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using PSMB1 antibody. Blue: DAPI for nuclear staining.)

PSMB1, Polyclonal Antibody (Cat# AAA28158)

Full Name

PSMB1 Polyclonal Antibody

Gene Names

PSMB1; HC5; PSC5; PMSB1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of PC-3 cells using anti-RBM15 antibody (AAA19815).Overlay histogram showing PC-3 cells stained with AAA19815 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15 Antibody (AAA19815, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of RBM15 using anti-RBM15 antibody (AAA19815).RBM15 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBM15 Antibody (AAA19815) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15 antigen affinity purified polyclonal antibody (#AAA19815) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBM15 at approximately 107 kDa. The expected band size for RBM15 is at 107 kDa.)

RBM15, Polyclonal Antibody (Cat# AAA19815)

Full Name

Anti-RBM15 Antibody Picoband

Gene Names

RBM15; OTT; OTT1; SPEN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-SPT5/SUPT5H antibody (AAA19487).Overlay histogram showing THP-1 cells stained with AAA19487 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPT5/SUPT5H Antibody (AAA19487, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SPT5/SUPT5H, Polyclonal Antibody (Cat# AAA19487)

Full Name

Anti-SPT5/SUPT5H Antibody Picoband

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti-RALBP1 antibody (AAA19684).Overlay histogram showing SiHa cells stained with AAA19684 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RALBP1 Antibody (AAA19684, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RALBP1, Monoclonal Antibody (Cat# AAA19684)

Full Name

Anti-RALBP1 Antibody Picoband (monoclonal, 2F9D3)

Gene Names

RALBP1; RIP1; RLIP1; RLIP76

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

LSM8, Monoclonal Antibody (Cat# AAA19721)

Full Name

Anti-LSM8 Antibody Picoband (monoclonal, 6B11)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

RAB7B, Monoclonal Antibody (Cat# AAA14798)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-INPPL1 antibody (AAA19364).Overlay histogram showing THP-1 cells stained with AAA19364 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- INPPL1 Antibody (AAA19364, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

INPPL1, Monoclonal Antibody (Cat# AAA19364)

Full Name

Anti-INPPL1 Antibody (monoclonal, 8C13)

Gene Names

INPPL1; OPSMD; SHIP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-PRPF18 antibody (AAA19988).Overlay histogram showing THP-1 cells stained with AAA19988 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRPF18 Antibody (AAA19988, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PRPF18 using anti-PRPF18 antibody (AAA19988).PRPF18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRPF18 Antibody (AAA19988) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (#AAA19988) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (42 kDa.)

PRPF18, Polyclonal Antibody (Cat# AAA19988)

Full Name

Anti-PRPF18 Antibody Picoband

Gene Names

PRPF18; PRP18; hPrp18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RT4 cells using anti-RAD51C antibody (AAA19762).Overlay histogram showing RT4 cells stained with AAA19762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD51C Antibody (AAA19762, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RAD51C using anti-RAD51C antibody (AAA19762).RAD51C was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: rat testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51C antigen affinity purified polyclonal antibody (#AAA19762) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD51C at approximately 42 kDa. The expected band size for RAD51C is at 42 kDa.)

RAD51C, Polyclonal Antibody (Cat# AAA19762)

Full Name

Anti-RAD51C Antibody Picoband

Gene Names

RAD51C; FANCO; BROVCA3; RAD51L2

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of RT4 cells using anti-BBS9 antibody (AAA19581).Overlay histogram showing RT4 cells stained with AAA19581 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BBS9 Antibody (AAA19581, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

BBS9, Polyclonal Antibody (Cat# AAA19581)

Full Name

Anti-BBS9 Antibody Picoband

Gene Names

BBS9; B1; D1; C18; PTHB1

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (EXOSC3 monoclonal antibody, Western Blot analysis of EXOSC3 expression in Hela NE.)

EXOSC3, Monoclonal Antibody (Cat# AAA25093)

Full Name

EXOSC3 (Exosome Complex Exonuclease RRP40, Ribosomal RNA-processing Protein 40, Exosome Component 3, p10, RRP40, CGI-102, MGC15120, MGC723, RP11-3J10.8, Rrp40p, bA3J10.7, hRrp40p, p10) (FITC)

Gene Names

EXOSC3; p10; PCH1B; RRP40; Rrp40p; CGI-102; hRrp-40; bA3J10.7

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-IFT52 antibody (AAA19977).Overlay histogram showing HepG2 cells stained with AAA19977 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT52 Antibody (AAA19977, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of IFT52 using anti-IFT52 antibody (AAA19977).IFT52 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT52 Antibody (AAA19977) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat testis tissue lysates,Lane 3: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT52 antigen affinity purified polyclonal antibody (#AAA19977) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFT52 at approximately 45 kDa. The expected band size for IFT52 is at 50 kDa.)

IFT52, Polyclonal Antibody (Cat# AAA19977)

Full Name

Anti-IFT52 Antibody Picoband

Gene Names

IFT52; NGD2; NGD5; CGI-53; C20orf9; dJ1028D15.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-NEDD4 antibody (AAA19413).Overlay histogram showing U87 cells stained with AAA19413 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4 Antibody (AAA19413, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NEDD4, Polyclonal Antibody (Cat# AAA19413)

Full Name

Anti-NEDD4 Antibody Picoband

Gene Names

NEDD4; RPF1; NEDD4-1

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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10000 results for "Recombinant Proteins" - showing 1550-1600

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STRN3, Polyclonal Antibody (Cat# AAA28082)

PSMB1, Polyclonal Antibody (Cat# AAA28158)

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SPT5/SUPT5H, Polyclonal Antibody (Cat# AAA19487)

RALBP1, Monoclonal Antibody (Cat# AAA19684)

LSM8, Monoclonal Antibody (Cat# AAA19721)

RAB7B, Monoclonal Antibody (Cat# AAA14798)

INPPL1, Monoclonal Antibody (Cat# AAA19364)

PRPF18, Polyclonal Antibody (Cat# AAA19988)

RAD51C, Polyclonal Antibody (Cat# AAA19762)

BBS9, Polyclonal Antibody (Cat# AAA19581)

EXOSC3, Monoclonal Antibody (Cat# AAA25093)

IFT52, Polyclonal Antibody (Cat# AAA19977)

NEDD4, Polyclonal Antibody (Cat# AAA19413)

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