Search results for "Recombinant Proteins"

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PMPCA/INPP5, Polyclonal Antibody (Cat# AAA19611)

Full Name

Anti-PMPCA/INPP5 Antibody Picoband

Gene Names

PMPCA; P-55; INPP5E; Alpha-MPP

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody at dilution of 1:100 (40x lens).)

CDH16, Polyclonal Antibody (Cat# AAA28211)

Full Name

CDH16 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using POP4 antibody at dilution of 1:100 (40x lens).)

POP4, Polyclonal Antibody (Cat# AAA28182)

Full Name

POP4 Polyclonal Antibody

Gene Names

POP4; RPP29

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)

RPS5, Monoclonal Antibody (Cat# AAA14776)

Full Name

RPS5 (40S Ribosomal Protein S5)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

BANF1, Monoclonal Antibody (Cat# AAA26443)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (HRP)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of HBD using anti-HBD antibody (AAA19142).HBD was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HBD Antibody (AAA19142) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

HBD, Polyclonal Antibody (Cat# AAA19142)

Full Name

Anti-HBD Picoband Antibody

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of DDB1 using anti-DDB1 antibody (AAA11660).DDB1 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DDB1 Antibody (AAA11660) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

DDB1, Polyclonal Antibody (Cat# AAA11660)

Full Name

Anti-DDB1 Antibody

Gene Names

DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitating RPL27 in Hela whole cell lysate .Western blot analysis of RPL27 using anti-RPL27 antibody (AAA19932).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-RPL27 antibody in Hela whole cell lysate.Lane 3: anti-RPL27 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (AAA19932) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL27 Antibody (AAA19932, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 2. Western blot analysis of RPL27 using anti-RPL27 antibody (AAA19932).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat thymus tissue lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse thymus tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (#AAA19932) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: human Hela whole cell lysates,Lane 8: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (#AAA19932) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPL27 at approximately 16 kDa. The expected band size for RPL27 is at 16 kDa.)

RPL27, Polyclonal Antibody (Cat# AAA19932)

Full Name

Anti-RPL27 Antibody Picoband

Gene Names

RPL27; L27

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of NIH/3T3 cells using anti-Hcn2 antibody (AAA19781).Overlay histogram showing NIH/3T3 cells stained with AAA19781 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hcn2 Antibody (AAA19781, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Hcn2 using anti-Hcn2 antibody (AAA19781).Hcn2 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hcn2 Antibody (AAA19781) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hcn2 antigen affinity purified polyclonal antibody (#AAA19781) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Hcn2 at approximately 110 kDa. The expected band size for Hcn2 is at 97-110 kDa.)

Hcn2, Polyclonal Antibody (Cat# AAA19781)

Full Name

Anti-Hcn2 Antibody Picoband

Gene Names

Hcn2; HAC1; trls; BCNG2

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of GHITM using anti-GHITM antibody (AAA19621).GHITM was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-GHITM Antibody (AAA19621) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

GHITM, Polyclonal Antibody (Cat# AAA19621)

Full Name

Anti-GHITM Antibody Picoband

Gene Names

GHITM; DERP2; MICS1; My021; PTD010; TMBIM5; HSPC282

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

SAV1, Monoclonal Antibody (Cat# AAA24945)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (Biotin)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse pancreas using PNLIPRP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

PNLIPRP1, Polyclonal Antibody (Cat# AAA28353)

Full Name

PNLIPRP1 Rabbit pAb

Gene Names

PNLIPRP1; PLRP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

DNAJC10, Monoclonal Antibody (Cat# AAA24489)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) APC

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in Hs 181.Tes.)

SMAD3, Monoclonal Antibody (Cat# AAA26562)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (PE)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRB antibody (AAA19985).Overlay histogram showing HCT116 cells stained with AAA19985 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRB Antibody (AAA19985, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of SRPRB using anti-SRPRB antibody (AAA19985).SRPRB was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRB Antibody (AAA19985) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SRPRB Antibody (AAA19985) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRB antigen affinity purified polyclonal antibody (#AAA19985) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SRPRB at approximately 30 kDa. The expected band size for SRPRB is at 30 kDa.)

SRPRB, Polyclonal Antibody (Cat# AAA19985)

Full Name

Anti-SRPRB Antibody Picoband

Gene Names

SRPRB; APMCF1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-MIPEP antibody (AAA19856).Overlay histogram showing MCF-7 cells stained with AAA19856 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIPEP Antibody (AAA19856, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MIPEP using anti-MIPEP antibody (AAA19856).MIPEP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIPEP Antibody (AAA19856) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIPEP antigen affinity purified polyclonal antibody (#AAA19856) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIPEP at approximately 80 kDa. The expected band size for MIPEP is at 80 kDa.)

MIPEP, Polyclonal Antibody (Cat# AAA19856)

Full Name

Anti-MIPEP Antibody Picoband

Gene Names

MIPEP; MIP; HMIP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 6. Western blot analysis of MVK using anti-MVK antibody (AAA19776).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVK antigen affinity purified polyclonal antibody (AAA19776) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for MVK at approximately 42 kDa. The expected band size for MVK is at 42 kDa.)

IF (Immunofluorescence) (Figure 4. IF analysis of MVK using anti-MVK antibody (AAA19776).MVK was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MVK Antibody (AAA19776) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MVK Antibody (AAA19776) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVK antigen affinity purified polyclonal antibody (#AAA19776) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MVK at approximately 42 kDa. The expected band size for MVK is at 42 kDa.)

MVK, Polyclonal Antibody (Cat# AAA19776)

Full Name

Anti-MVK Antibody Picoband

Gene Names

MVK; MK; LRBP; MVLK; POROK3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

IGF2, ELISA Kit (Cat# AAA17880)

Full Name

Rat IGF2 ELISA Kit

Gene Names

Igf2; IGFII; RNIGF2

Reactivity

Rat

Applications

Sandwich ELISA

Pricing

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FKBP4, Polyclonal Antibody (Cat# AAA28189)

Full Name

FKBP4 Polyclonal Antibody

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)

STIP1, Monoclonal Antibody (Cat# AAA25917)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (AP)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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ICC (Immunocytochemistry) (Anti-SHC antibody, AAA11580, ICCICC: A549 Cell)

SHC, Polyclonal Antibody (Cat# AAA11580)

Full Name

Anti-SHC antibody

Gene Names

SHC1; SHC; SHCA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Figure 7. Western blot analysis of PLS1 using anti-PLS1 antibody (AAA19893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

IF (Immunofluorescence) (Figure 5. IF analysis of PLS1 using anti-PLS1 antibody (AAA19893).PLS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLS1 Antibody (AAA19893) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: rat small intestine tissue lysates,Lane 6: rat stomach tissue lysates,Lane 7: mouse small intestine tissue lysates,Lane 8: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (#AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)

PLS1, Polyclonal Antibody (Cat# AAA19893)

Full Name

Anti-PLS1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

SBNO1, Polyclonal Antibody (Cat# AAA19633)

Full Name

Anti-SBNO1 Antibody Picoband

Gene Names

SBNO1; Sno; MOP3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using PEX14 antibody. Blue: DAPI for nuclear staining.)

PEX14, Polyclonal Antibody (Cat# AAA28157)

Full Name

PEX14

Gene Names

PEX14; NAPP2; PBD13A; Pex14p; dJ734G22.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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WB (Western Blot) (RAB33B monoclonal antibody. Western Blot analysis of RAB33B expression in NIH/3T3.)

RAB33B, Monoclonal Antibody (Cat# AAA25220)

Full Name

RAB33B (Ras-related Protein Rab-33B, SMC2) (FITC)

Gene Names

RAB33B; SMC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using SULT2A1 Antibody at dilution of 1:200 (40x lens).)

SULT2A1, Polyclonal Antibody (Cat# AAA28271)

Full Name

SULT2A1 Polyclonal Antibody

Gene Names

SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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<< Previous Next >>

WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)

DDX54, Monoclonal Antibody (Cat# AAA25076)

Full Name

DDX54 (ATP-dependent RNA Helicase DDX54, ATP-dependent RNA Helicase DP97, DEAD Box RNA Helicase 97kD, DEAD Box Protein 54, MGC2835) (FITC)

Gene Names

DDX54; DP97

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PACSIN1 antibody (AAA19863).Overlay histogram showing K562 cells stained with AAA19863 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN1 Antibody (AAA19863, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PACSIN1 using anti-PACSIN1 antibody (AAA19863) and anti-Beta Tubulin antibody (M01857-3).PACSIN1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN1 Antibody (AAA19863) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN1 antigen affinity purified polyclonal antibody (#AAA19863) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PACSIN1 at approximately 51 kDa. The expected band size for PACSIN1 is at 51 kDa.)

PACSIN1, Polyclonal Antibody (Cat# AAA19863)

Full Name

Anti-PACSIN1 Antibody Picoband

Gene Names

PACSIN1; SDPI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

SARS2, Polyclonal Antibody (Cat# AAA19598)

Full Name

Anti-SARS2 Antibody Picoband

Gene Names

SARS2; SYS; SARS; SERS; SARSM; SerRS; SerRSmt; mtSerRS

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

GROS1, Monoclonal Antibody (Cat# AAA24228)

Full Name

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (AP)

Gene Names

P3H1; OI8; GROS1; LEPRE1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

RPN1, Polyclonal Antibody (Cat# AAA28249)

Full Name

RPN1 Polyclonal Antibody

Gene Names

RPN1; OST1; RBPH1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using RALGDS antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

RALGDS, Polyclonal Antibody (Cat# AAA28359)

Full Name

RALGDS Rabbit pAb

Gene Names

RALGDS; RGF; RGDS; RalGEF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-OXSM antibody (AAA19976).Overlay histogram showing MCF-7 cells stained with AAA19976 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OXSM Antibody (AAA19976, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of OXSM using anti-OXSM antibody (AAA19976).OXSM was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OXSM Antibody (AAA19976) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OXSM Antibody (AAA19976) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OXSM Antibody (AAA19976) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OXSM Antibody (AAA19976) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXSM antigen affinity purified polyclonal antibody (#AAA19976) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (49 kDa.)

OXSM, Polyclonal Antibody (Cat# AAA19976)

Full Name

Anti-OXSM Antibody Picoband

Gene Names

OXSM; KS; KASI; FASN2D

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PGC antibody (AAA19725).Overlay histogram showing HepG2 cells stained with AAA19725 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PGC Antibody (AAA19725, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PGC using anti-PGC antibody (AAA19725).PGC was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGC Antibody (AAA19725) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGC Antibody (AAA19725) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PGC Antibody (AAA19725) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGC antigen affinity purified polyclonal antibody (#AAA19725) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (32,38 kDa.)

PGC, Polyclonal Antibody (Cat# AAA19725)

Full Name

Anti-PGC Antibody Picoband

Gene Names

PGC; PEPC; PGII

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A549 cells using anti-SHKBP1 antibody (AAA19991).Overlay histogram showing A549 cells stained with AAA19991 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHKBP1 Antibody (AAA19991, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SHKBP1 using anti-SHKBP1 antibody (AAA19991).SHKBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHKBP1 antigen affinity purified polyclonal antibody (#AAA19991) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHKBP1 at approximately 76 kDa. The expected band size for SHKBP1 is at 76 kDa.)

SHKBP1, Polyclonal Antibody (Cat# AAA19991)

Full Name

Anti-SHKBP1 Antibody Picoband

Gene Names

SHKBP1; Sb1; PP203

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MELTF using anti-MELTF antibody (AAA19980).MELTF was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MELTF Antibody (AAA19980) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat C6 whole cell lysates,Lane 3: mouse skin tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antigen affinity purified polyclonal antibody (#AAA19980) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human A431 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antigen affinity purified polyclonal antibody (#AAA19980) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MELTF at approximately 90 kDa. The expected band size for MELTF is at 80 kDa.)

MELTF, Polyclonal Antibody (Cat# AAA19980)

Full Name

Anti-MELTF Antibody Picoband

Gene Names

MFI2; MTF1; CD228; MAP97

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of MCF-7 cells using anti-PAPSS2 antibody (AAA19850).Overlay histogram showing MCF-7 cells stained with AAA19850 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAPSS2 Antibody (AAA19850, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PAPSS2 using anti-PAPSS2 antibody (AAA19850).PAPSS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PAPSS2 Antibody (AAA19850) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse liver tissue lysates,Lane 5: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAPSS2 antigen affinity purified polyclonal antibody (#AAA19850) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAPSS2 at approximately 70 kDa. The expected band size for PAPSS2 is at 70 kDa.)

PAPSS2, Polyclonal Antibody (Cat# AAA19850)

Full Name

Anti-PAPSS2 Antibody Picoband

Gene Names

PAPSS2; SK2; BCYM4; ATPSK2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U20S cells using anti-TMEM87A antibody (AAA19997).Overlay histogram showing U20S cells stained with AAA19997 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM87A Antibody (AAA19997, 1ug/1x106 cells) for 30 min at 20 degree C. PE conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 9. IF analysis of TMEM87A using anti-TMEM87A antibody (AAA19997).TMEM87A was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TMEM87A Antibody (AAA19997) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MDA-MB-453 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human MCF-7 tissue lysates,Lane 5: rat brain tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM87A antigen affinity purified polyclonal antibody (#AAA19997) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TMEM87A at approximately 70 kDa. The expected band size for TMEM87A is at 63 kDa.)

TMEM87A, Polyclonal Antibody (Cat# AAA19997)

Full Name

Anti-TMEM87A Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-POC1A antibody (AAA19908).Overlay histogram showing PC-3 cells stained with AAA19908 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POC1A Antibody (AAA19908, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of POC1A using anti-POC1A antibody (AAA19908).POC1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POC1A Antibody (AAA19908) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POC1A Antibody (AAA19908) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POC1A Antibody (AAA19908) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POC1A Antibody (AAA19908) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human THP-1 whole cell lysates,Lane 3: human U251 whole cell lysates,Lane 4: rat testis tissue lysates,Lane 5: rat thymus tissue lysates,Lane 6: mouse testis tissue lysates,Lane 7: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POC1A antigen affinity purified polyclonal antibody (#AAA19908) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POC1A at approximately 40 kDa. The expected band size for POC1A is at 45 kDa.)

POC1A, Polyclonal Antibody (Cat# AAA19908)

Full Name

Anti-POC1A Antibody Picoband

Gene Names

POC1A; PIX2; SOFT; WDR51A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Caco-2 cells using anti-MATK antibody (AAA19767).Overlay histogram showing Caco-2 cells stained with AAA19767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MATK Antibody (AAA19767, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of MATK using anti-MATK antibody (AAA19767).MATK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MATK Antibody (AAA19767) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse thymus tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATK antigen affinity purified polyclonal antibody (#AAA19767) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MATK at approximately 52 kDa. The expected band size for MATK is at 56 kDa.)

MATK, Polyclonal Antibody (Cat# AAA19767)

Full Name

Anti-MATK Antibody Picoband

Gene Names

MATK; CHK; CTK; HYL; Lsk; HYLTK; HHYLTK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

CAD, Polyclonal Antibody (Cat# AAA19398)

Full Name

Anti-CAD Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

< >

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WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)

DDX54, Monoclonal Antibody (Cat# AAA24484)

Full Name

DDX54 (ATP-dependent RNA Helicase DDX54, ATP-dependent RNA Helicase DP97, DEAD Box RNA Helicase 97kD, DEAD Box Protein 54, MGC2835) APC

Gene Names

DDX54; DP97

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

GROS1, Monoclonal Antibody (Cat# AAA25410)

Full Name

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (HRP)

Gene Names

P3H1; OI8; GROS1; LEPRE1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.)

SMAD3, Monoclonal Antibody (Cat# AAA25983)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (AP)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)

SMAD4, Monoclonal Antibody (Cat# AAA24955)

Full Name

SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (Biotin)

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

<< Previous Next >>

Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2168) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Emerin, Monoclonal Antibody (Cat# AAA23899)

Full Name

Emerin (Papillary Thyroid Carcinoma and EDMD Marker)

Gene Names

EMD; STA; EDMD; LEMD5

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

< >

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-NIT2 antibody (AAA19853).Overlay histogram showing A549 cells stained with AAA19853 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NIT2 Antibody (AAA19853, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NIT2 using anti-NIT2 antibody (AAA19853).NIT2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIT2 antigen affinity purified polyclonal antibody (#AAA19853) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NIT2 at approximately 33 kDa. The expected band size for NIT2 is at 33 kDa.)

NIT2, Polyclonal Antibody (Cat# AAA19853)

Full Name

Anti-NIT2 Antibody Picoband

Gene Names

NIT2; HEL-S-8a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

SND1, Monoclonal Antibody (Cat# AAA19699)

Full Name

Anti-SND1 Antibody Picoband (monoclonal, 5F5E9)

Gene Names

SND1; p100; TDRD11

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

Periostin, ELISA Kit (Cat# AAA17869)

Full Name

Human Periostin ELISA Kit

Gene Names

POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin

Reactivity

Human

Applications

Sandwich ELISA

Pricing

PDIA6, Recombinant Protein (Cat# AAA11803)

Full Name

PDIA6, 20-440aa, Human, His tag, E Coli

Gene Names

PDIA6; P5; ERP5; TXNDC7

Applications

SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

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10000 results for "Recombinant Proteins" - showing 1500-1550

PMPCA/INPP5, Polyclonal Antibody (Cat# AAA19611)

CDH16, Polyclonal Antibody (Cat# AAA28211)

POP4, Polyclonal Antibody (Cat# AAA28182)

RPS5, Monoclonal Antibody (Cat# AAA14776)

BANF1, Monoclonal Antibody (Cat# AAA26443)

HBD, Polyclonal Antibody (Cat# AAA19142)

DDB1, Polyclonal Antibody (Cat# AAA11660)

RPL27, Polyclonal Antibody (Cat# AAA19932)

Hcn2, Polyclonal Antibody (Cat# AAA19781)

GHITM, Polyclonal Antibody (Cat# AAA19621)

SAV1, Monoclonal Antibody (Cat# AAA24945)

PNLIPRP1, Polyclonal Antibody (Cat# AAA28353)

DNAJC10, Monoclonal Antibody (Cat# AAA24489)

SMAD3, Monoclonal Antibody (Cat# AAA26562)

SRPRB, Polyclonal Antibody (Cat# AAA19985)

MIPEP, Polyclonal Antibody (Cat# AAA19856)

MVK, Polyclonal Antibody (Cat# AAA19776)

IGF2, ELISA Kit (Cat# AAA17880)

FKBP4, Polyclonal Antibody (Cat# AAA28189)

STIP1, Monoclonal Antibody (Cat# AAA25917)

SHC, Polyclonal Antibody (Cat# AAA11580)

PLS1, Polyclonal Antibody (Cat# AAA19893)

SBNO1, Polyclonal Antibody (Cat# AAA19633)

PEX14, Polyclonal Antibody (Cat# AAA28157)

RAB33B, Monoclonal Antibody (Cat# AAA25220)

SULT2A1, Polyclonal Antibody (Cat# AAA28271)

DDX54, Monoclonal Antibody (Cat# AAA25076)

PACSIN1, Polyclonal Antibody (Cat# AAA19863)

SARS2, Polyclonal Antibody (Cat# AAA19598)

GROS1, Monoclonal Antibody (Cat# AAA24228)

RPN1, Polyclonal Antibody (Cat# AAA28249)

RALGDS, Polyclonal Antibody (Cat# AAA28359)

OXSM, Polyclonal Antibody (Cat# AAA19976)

PGC, Polyclonal Antibody (Cat# AAA19725)

SHKBP1, Polyclonal Antibody (Cat# AAA19991)

MELTF, Polyclonal Antibody (Cat# AAA19980)

PAPSS2, Polyclonal Antibody (Cat# AAA19850)

TMEM87A, Polyclonal Antibody (Cat# AAA19997)

POC1A, Polyclonal Antibody (Cat# AAA19908)

MATK, Polyclonal Antibody (Cat# AAA19767)

CAD, Polyclonal Antibody (Cat# AAA19398)

DDX54, Monoclonal Antibody (Cat# AAA24484)

GROS1, Monoclonal Antibody (Cat# AAA25410)

SMAD3, Monoclonal Antibody (Cat# AAA25983)

SMAD4, Monoclonal Antibody (Cat# AAA24955)

Emerin, Monoclonal Antibody (Cat# AAA23899)

NIT2, Polyclonal Antibody (Cat# AAA19853)

SND1, Monoclonal Antibody (Cat# AAA19699)

Periostin, ELISA Kit (Cat# AAA17869)

PDIA6, Recombinant Protein (Cat# AAA11803)

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