Search results for "Recombinant Proteins"

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GNG7, Polyclonal Antibody (Cat# AAA19335)

Full Name

Anti-GNG7 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-TMPRSS3 antibody (AAA19289).Overlay histogram showing A431 cells stained with AAA19289 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS3 Antibody (AAA19289, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TMPRSS3, Polyclonal Antibody (Cat# AAA19289)

Full Name

Anti-TMPRSS3 Antibody

Gene Names

TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ACAT1 antibody (AAA19765).Overlay histogram showing MCF-7 cells stained with AAA19765 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAT1 Antibody (AAA19765, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of ACAT1 using anti-ACAT1 antibody (AAA19765).ACAT1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat skeletal muscle tissue lysates,Lane 7: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT1 antigen affinity purified polyclonal antibody (#AAA19765) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACAT1 at approximately 40 kDa. The expected band size for ACAT1 is at 40 kDa.)

ACAT1, Polyclonal Antibody (Cat# AAA19765)

Full Name

Anti-ACAT1 Antibody Picoband

Gene Names

ACAT1; T2; MAT; ACAT; THIL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of TRIM24 using anti-TRIM24 antibody (AAA19707).TRIM24 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-TRIM24 Antibody (AAA19707) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

TRIM24, Monoclonal Antibody (Cat# AAA19707)

Full Name

Anti-TRIM24 Antibody Picoband (monoclonal, 4G6C2)

Gene Names

TRIM24; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-TCEB2/Elongin-B/ELOB antibody (AAA19645).Overlay histogram showing JK cells stained with AAA19645 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TCEB2/Elongin-B/ELOB Antibody (AAA19645, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TCEB2/Elongin-B/ELOB, Polyclonal Antibody (Cat# AAA19645)

Full Name

Anti-TCEB2/Elongin-B/ELOB Antibody Picoband

Gene Names

ELOB; SIII; TCEB2

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody at dilution of 1:100 (40x lens).)

MDC1, Polyclonal Antibody (Cat# AAA28205)

Full Name

MDC1 Polyclonal Antibody

Gene Names

MDC1; NFBD1

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using SULT2B1 antibody.)

SULT2B1, Polyclonal Antibody (Cat# AAA28196)

Full Name

SULT2B1 Polyclonal Antibody

Gene Names

SULT2B1; HSST2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

MPP1, Monoclonal Antibody (Cat# AAA26079)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (APC)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in HeLa.)

RASSF8, Monoclonal Antibody (Cat# AAA25224)

Full Name

RASSF8 (Ras Association Domain-containing Protein 8, Carcinoma-associated Protein HOJ-1, C12orf2) (FITC)

Gene Names

RASSF8; HOJ1; C12orf2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody. Western blot was performed from the immunoprecipitate using RFC4 antibody at a dilition of 1:1000.)

RFC4, Polyclonal Antibody (Cat# AAA28137)

Full Name

RFC4 Polyclonal Antibody

Gene Names

RFC4; A1; RFC37

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

PAXIP1, Polyclonal Antibody (Cat# AAA28350)

Full Name

PAXIP1 Rabbit pAb

Gene Names

PAXIP1; PTIP; TNRC2; CAGF28; CAGF29; PACIP1; PAXIP1L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse spleen using LILRB4 Rabbit pAb (AAA28090) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)

LILRB4, Antibody (Cat# AAA28090)

Full Name

LILRB4

Gene Names

LILRB4; ILT3; LIR5; CD85K; ILT-3; LIR-5

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung using FADD Rabbit mAb (AAA28538) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

FADD, Monoclonal Antibody (Cat# AAA28538)

Full Name

FADD Rabbit mAb

Reactivity

Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U251 cells using anti-RPS5 antibody (AAA19817).Overlay histogram showing U251 cells stained with AAA19817 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS5 Antibody (AAA19817, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of RPS5 using anti-RPS5 antibody (AAA19817).RPS5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS5 antigen affinity purified polyclonal antibody (#AAA19817) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS5 at approximately 23 kDa. The expected band size for RPS5 is at 23 kDa.)

RPS5, Polyclonal Antibody (Cat# AAA19817)

Full Name

Anti-RPS5 Antibody Picoband

Gene Names

RPS5; S5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RTCB antibody (AAA19833).Overlay histogram showing Hela cells stained with AAA19833 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCB Antibody (AAA19833, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RTCB using anti-RTCB antibody (AAA19833).RTCB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (#AAA19833) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.)

RTCB, Polyclonal Antibody (Cat# AAA19833)

Full Name

Anti-RTCB Antibody Picoband

Gene Names

RTCB; FAAP; HSPC117; C22orf28; DJ149A16.6

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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TRIM16, Polyclonal Antibody (Cat# AAA19608)

Full Name

Anti-TRIM16 Antibody Picoband

Gene Names

TRIM16; EBBP

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HepG2 cells using anti-MYH14 antibody (AAA19818).Overlay histogram showing HepG2 cells stained with AAA19818 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYH14 Antibody (AAA19818, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of MYH14 using anti-MYH14 antibody (AAA19818).MYH14 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U-87MG whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (#AAA19818) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.)

MYH14, Polyclonal Antibody (Cat# AAA19818)

Full Name

Anti-MYH14 Antibody Picoband

Gene Names

MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

SAV1, Monoclonal Antibody (Cat# AAA25831)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (PE)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)

SSH3, Monoclonal Antibody (Cat# AAA25264)

Full Name

SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (FITC)

Gene Names

SSH3; SSH3L

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged SMAD3 is approximately 3ng/ml as a capture antibody.)

SMAD3, Monoclonal Antibody (Cat# AAA26297)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (FITC)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in NIH/3T3.)

CPSF3, Monoclonal Antibody (Cat# AAA14795)

Full Name

CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73)

Gene Names

CPSF3; CPSF73; CPSF-73

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

SNX1, Monoclonal Antibody (Cat# AAA24962)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (Biotin)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-SCLY antibody (AAA19917).Overlay histogram showing SH-SY5Y cells stained with AAA19917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCLY Antibody (AAA19917, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SCLY using anti-SCLY antibody (AAA19917).SCLY was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SCLY Antibody (AAA19917) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human SH-SY5Y whole cell lysates,Lane 4: human THP-1 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCLY antigen affinity purified polyclonal antibody (#AAA19917) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCLY at approximately 48 kDa. The expected band size for SCLY is at 48 kDa.)

SCLY, Polyclonal Antibody (Cat# AAA19917)

Full Name

Anti-SCLY Antibody Picoband

Gene Names

SCLY; SCL; hSCL

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MIA3 antibody (AAA19862).Overlay histogram showing 293T cells stained with AAA19862 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIA3 Antibody (AAA19862, 1ug/1x106 cells) for 30 min at 20 degree C. DyLightMIA3 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MIA3 using anti-MIA3 antibody (AAA19862).MIA3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human Hacat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIA3 antigen affinity purified polyclonal antibody (#AAA19862) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIA3 at approximately 300 kDa. The expected band size for MIA3 is at 300 kDa.)

MIA3, Polyclonal Antibody (Cat# AAA19862)

Full Name

Anti-MIA3 Antibody Picoband

Gene Names

MIA3; ARNT; D320; TANGO; TANGO1; UNQ6077

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Hela cells using anti-MOSPD2 antibody (AAA20007).Overlay histogram showing Hela cells stained with AAA20007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOSPD2 Antibody (AAA20007, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MOSPD2 using anti-MOSPD2 antibody (AAA20007).MOSPD2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U251 whole cell lysates,Lane 3: rat C6 whole cell lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse Neuro-2a whole cell lysates,Lane 7: mouse HEPA1-6 whole cell lysates,Lane 8: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOSPD2 antigen affinity purified polyclonal antibody (#AAA20007) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MOSPD2 at approximately 60 kDa. The expected band size for MOSPD2 is at 60 kDa.)

MOSPD2, Polyclonal Antibody (Cat# AAA20007)

Full Name

Anti-MOSPD2 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of U87 cells using anti-RBMS1 antibody (AAA19911).Overlay histogram showing U87 cells stained with AAA19911 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBMS1 Antibody (AAA19911, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of RBMS1 using anti-RBMS1 antibody (AAA19911).RBMS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBMS1 Antibody (AAA19911) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBMS1 antigen affinity purified polyclonal antibody (#AAA19911) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBMS1 at approximately 50 kDa. The expected band size for RBMS1 is at 45 kDa.)

RBMS1, Polyclonal Antibody (Cat# AAA19911)

Full Name

Anti-RBMS1 Antibody Picoband

Gene Names

RBMS1; YC1; MSSP; SCR2; HCC-4; MSSP-1; MSSP-2; MSSP-3; C2orf12

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-AKAP 95/AKAP8 antibody (AAA19538).Overlay histogram showing A431 cells stained with AAA19538 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

AKAP 95/AKAP8, Polyclonal Antibody (Cat# AAA19538)

Full Name

Anti-AKAP 95/AKAP8 Antibody Picoband

Gene Names

AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FKBP135/FKBP15, Polyclonal Antibody (Cat# AAA19620)

Full Name

Anti-FKBP135/FKBP15 Antibody Picoband

Gene Names

FKBP15; FKBP133; PPP1R76; KIAA0674

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

EFHD1, Monoclonal Antibody (Cat# AAA25673)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (PE)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

HCLS1, Monoclonal Antibody (Cat# AAA25711)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (PE)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

GNG2, Polyclonal Antibody (Cat# AAA19312)

Full Name

Anti-GNG2 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SiHa cells using anti-PCYOX1 antibody (AAA19954).Overlay histogram showing SiHa cells stained with AAA19954 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCYOX1 Antibody (AAA19954, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PCYOX1 using anti-PCYOX1 antibody (AAA19954).PCYOX1 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCYOX1 Antibody (AAA19954) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCYOX1 Antibody (AAA19954) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCYOX1 Antibody (AAA19954) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCYOX1 Antibody (AAA19954) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human SiHa whole cell lysates,Lane 5: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCYOX1 antigen affinity purified polyclonal antibody (#AAA19954) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCYOX1 at approximately 57 kDa. The expected band size for PCYOX1 is at 57 kDa.)

PCYOX1, Polyclonal Antibody (Cat# AAA19954)

Full Name

Anti-PCYOX1 Antibody Picoband

Gene Names

PCYOX1; PCL1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Caco-2 cells using anti-EIF3B antibody (AAA19811).Overlay histogram showing Caco-2 cells stained with AAA19811 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF3B Antibody (AAA19811, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of EIF3B using anti-EIF3B antibody (AAA19811).EIF3B was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-EIF3B Antibody (AAA19811) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: human MCF-7 whole cell lysates,Lane 6: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3B antigen affinity purified polyclonal antibody (#AAA19811) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 115 kDa.)

EIF3B, Polyclonal Antibody (Cat# AAA19811)

Full Name

Anti-EIF3B Antibody Picoband

Gene Names

EIF3B; PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-MAFF antibody (AAA19588).Overlay histogram showing A431 cells stained with AAA19588 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAFF Antibody (AAA19588, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MAFF, Polyclonal Antibody (Cat# AAA19588)

Full Name

Anti-MAFF Antibody Picoband

Gene Names

MAFF; U-MAF; hMafF

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in PC-12.)

LRRN1, Monoclonal Antibody (Cat# AAA26550)

Full Name

LRRN1 (Leucine Rich Repeat Neuronal 1, FIGLER3, KIAA1497, NLRR-1, LRCH4) (HRP)

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in NIH/3T3.)

NUP62, Monoclonal Antibody (Cat# AAA25184)

Full Name

NUP62 (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62) (FITC)

Gene Names

NUP62; p62; IBSN; SNDI

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

< >

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WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

MPP1, Monoclonal Antibody (Cat# AAA26345)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (FITC)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)

DAAM1, Monoclonal Antibody (Cat# AAA25661)

Full Name

DAAM1 (Disheveled-associated Activator Of Morphogenesis 1, KIAA0666) (PE)

Reactivity

Human, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of JK cells using anti-LRRC59 antibody (AAA19935).Overlay histogram showing JK cells stained with AAA19935 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRC59 Antibody (AAA19935, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of LRRC59 using anti-LRRC59 antibody (AAA19935).LRRC59 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LRRC59 Antibody (AAA19935) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat RH-35 whole cell lysates,Lane 3: mouse liver tissue lysates,Lane 4: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (#AAA19935) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human Caco-2 whole cell lysates,Lane 6: human A431 whole cell lysates,Lane 7: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC59 antigen affinity purified polyclonal antibody (#AAA19935) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRC59 at approximately 35 kDa. The expected band size for LRRC59 is at 35 kDa.)

LRRC59, Polyclonal Antibody (Cat# AAA19935)

Full Name

Anti-LRRC59 Antibody Picoband

Gene Names

LRRC59; p34; PRO1855

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-Symplekin/SYMPK antibody (AAA19851).Overlay histogram showing U87 cells stained with AAA19851 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Symplekin/SYMPK Antibody (AAA19851, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (AAA19851) and anti-Tubulin Alpha antibody (M03989-3).Symplekin/SYMPK was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: rat testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Symplekin/SYMPK antigen affinity purified polyclonal antibody (#AAA19851) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Symplekin/SYMPK at approximately 150 kDa. The expected band size for Symplekin/SYMPK is at 141 kDa.)

Symplekin/SYMPK, Polyclonal Antibody (Cat# AAA19851)

Full Name

Anti-Symplekin/SYMPK Antibody Picoband

Gene Names

SYMPK; SPK; SYM; FLJ27092

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 14. Flow Cytometry analysis of 293T cells using anti-STAG2 antibody (AAA19795).Overlay histogram showing 293T cells stained with AAA19795 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAG2 Antibody (AAA19795, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 11. IF analysis of STAG2 using anti-STAG2 antibody (AAA19795) and anti-Tubulin Alpha antibody (M03989-3).STAG2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STAG2 Antibody (AAA19795) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SiHa whole cell lysates,Lane 3: huamn RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA2/STAG2 antigen affinity purified polyclonal antibody (#AAA19795) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SA2/STAG2 at approximately 141 kDa. The expected band size for SA2/STAG2 is at 141 kDa.)

SA2/STAG2, Polyclonal Antibody (Cat# AAA19795)

Full Name

Anti-SA2/STAG2 Antibody Picoband

Gene Names

STAG2; SA2; SA-2; SCC3B; bA517O1.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

CXCL15, ELISA Kit (Cat# AAA27879)

Full Name

Mouse CXCL15 ELISA Kit

Gene Names

Cxcl15; Il8; weche; Scyb15; lungkine

Reactivity

Mouse

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using TAPBP Antibody at dilution of 1:200 (40x lens).)

TAPBP, Polyclonal Antibody (Cat# AAA28097)

Full Name

TAPBP Polyclonal Antibody

Gene Names

TAPBP; TPN; TAPA; TPSN; NGS17

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in HeLa.)

RASSF8, Monoclonal Antibody (Cat# AAA25814)

Full Name

RASSF8 (Ras Association Domain-containing Protein 8, Carcinoma-associated Protein HOJ-1, C12orf2) (PE)

Gene Names

RASSF8; HOJ1; C12orf2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RASSF8 monoclonal antibody Western Blot analysis of RASSF8 expression in HeLa.)

RASSF8, Monoclonal Antibody (Cat# AAA24632)

Full Name

RASSF8 (Ras Association Domain-containing Protein 8, Carcinoma-associated Protein HOJ-1, C12orf2) APC

Gene Names

RASSF8; HOJ1; C12orf2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

CFI, Recombinant Protein (Cat# AAA11715)

Full Name

Human CFI Protein, His Tag

Gene Names

CFI; FI; IF; KAF; AHUS3; C3BINA; C3b-INA

Purity

Purified from cell culture supernatant by affinity chromatography
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse brain using CAMK2G antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.)

CAMK2G, Polyclonal Antibody (Cat# AAA28433)

Full Name

CAMK2G Rabbit pAb

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Raji cells using anti-GGA3 antibody (AAA19494).Overlay histogram showing Raji cells stained with AAA19494 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA3 Antibody (AAA19494, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

GGA3, Polyclonal Antibody (Cat# AAA19494)

Full Name

Anti-GGA3 Antibody Picoband

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of SDF2L1 using anti-SDF2L1 antibody (AAA19635).SDF2L1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-SDF2L1 Antibody (AAA19635) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

SDF2L1, Polyclonal Antibody (Cat# AAA19635)

Full Name

Anti-SDF2L1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U2OS cells using anti-SART1 antibody (AAA19547).Overlay histogram showing U2OS cells stained with AAA19547 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SART1 Antibody (AAA19547, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SART1, Polyclonal Antibody (Cat# AAA19547)

Full Name

Anti-SART1 Antibody Picoband

Gene Names

SART1; Ara1; HOMS1; Snu66; SART1259; SNRNP110

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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10000 results for "Recombinant Proteins" - showing 1450-1500

GNG7, Polyclonal Antibody (Cat# AAA19335)

TMPRSS3, Polyclonal Antibody (Cat# AAA19289)

ACAT1, Polyclonal Antibody (Cat# AAA19765)

TRIM24, Monoclonal Antibody (Cat# AAA19707)

TCEB2/Elongin-B/ELOB, Polyclonal Antibody (Cat# AAA19645)

MDC1, Polyclonal Antibody (Cat# AAA28205)

SULT2B1, Polyclonal Antibody (Cat# AAA28196)

MPP1, Monoclonal Antibody (Cat# AAA26079)

RASSF8, Monoclonal Antibody (Cat# AAA25224)

RFC4, Polyclonal Antibody (Cat# AAA28137)

PAXIP1, Polyclonal Antibody (Cat# AAA28350)

LILRB4, Antibody (Cat# AAA28090)

FADD, Monoclonal Antibody (Cat# AAA28538)

RPS5, Polyclonal Antibody (Cat# AAA19817)

RTCB, Polyclonal Antibody (Cat# AAA19833)

TRIM16, Polyclonal Antibody (Cat# AAA19608)

MYH14, Polyclonal Antibody (Cat# AAA19818)

SAV1, Monoclonal Antibody (Cat# AAA25831)

SSH3, Monoclonal Antibody (Cat# AAA25264)

SMAD3, Monoclonal Antibody (Cat# AAA26297)

CPSF3, Monoclonal Antibody (Cat# AAA14795)

SNX1, Monoclonal Antibody (Cat# AAA24962)

SCLY, Polyclonal Antibody (Cat# AAA19917)

MIA3, Polyclonal Antibody (Cat# AAA19862)

MOSPD2, Polyclonal Antibody (Cat# AAA20007)

RBMS1, Polyclonal Antibody (Cat# AAA19911)

AKAP 95/AKAP8, Polyclonal Antibody (Cat# AAA19538)

FKBP135/FKBP15, Polyclonal Antibody (Cat# AAA19620)

EFHD1, Monoclonal Antibody (Cat# AAA25673)

HCLS1, Monoclonal Antibody (Cat# AAA25711)

GNG2, Polyclonal Antibody (Cat# AAA19312)

PCYOX1, Polyclonal Antibody (Cat# AAA19954)

EIF3B, Polyclonal Antibody (Cat# AAA19811)

MAFF, Polyclonal Antibody (Cat# AAA19588)

LRRN1, Monoclonal Antibody (Cat# AAA26550)

NUP62, Monoclonal Antibody (Cat# AAA25184)

MPP1, Monoclonal Antibody (Cat# AAA26345)

DAAM1, Monoclonal Antibody (Cat# AAA25661)

LRRC59, Polyclonal Antibody (Cat# AAA19935)

Symplekin/SYMPK, Polyclonal Antibody (Cat# AAA19851)

SA2/STAG2, Polyclonal Antibody (Cat# AAA19795)

CXCL15, ELISA Kit (Cat# AAA27879)

TAPBP, Polyclonal Antibody (Cat# AAA28097)

RASSF8, Monoclonal Antibody (Cat# AAA25814)

RASSF8, Monoclonal Antibody (Cat# AAA24632)

CFI, Recombinant Protein (Cat# AAA11715)

CAMK2G, Polyclonal Antibody (Cat# AAA28433)

GGA3, Polyclonal Antibody (Cat# AAA19494)

SDF2L1, Polyclonal Antibody (Cat# AAA19635)

SART1, Polyclonal Antibody (Cat# AAA19547)

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