Search results for "Recombinant Proteins"

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WB (Western Blot) (Western Blot analysis of CTNS expression in PC-12)

Cystinosin, Monoclonal Antibody (Cat# AAA25365)

Full Name

Cystinosin (CTNS) (HRP)

Gene Names

CTNS; PQLC4; SLC66A4; CTNS-LSB

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged SMAD2 is approximately 0.1ng/ml as a capture antibody.)

SMAD2, Monoclonal Antibody (Cat# AAA26029)

Full Name

SMAD2 (SMAD Family Member 2, JV18, JV18-1, MADH2, MADR2, MGC22139, MGC34440, hMAD-2, hSMAD2) (APC)

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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IHC (Immunohistochemistry) (Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

GYG1, Monoclonal Antibody (Cat# AAA24085)

Full Name

GYG1 (Glycogenin 1, Glycogenin-1, GN-1, GN1, GYG)

Gene Names

GYG1; GYG; GSD15

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

BAF60a, Polyclonal Antibody (Cat# AAA28224)

Full Name

BAF60a Polyclonal Antibody

Gene Names

Smarcd1; Baf60a; D15Kz1; AA407987

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

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Application Data (Detection limit for recombinant GST tagged STIP1 is 0.03 ng/ml as a capture antibody.)

STIP1, Monoclonal Antibody (Cat# AAA26414)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)

ACBD3, Monoclonal Antibody (Cat# AAA25593)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)

LDB3, Monoclonal Antibody (Cat# AAA25143)

Full Name

LDB3 (LIM Domain-binding Protein 3, Protein Cypher, Z-band Alternatively Spliced PDZ-motif Protein, KIAA0613, ZASP) (FITC)

Gene Names

LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CHCHD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CHCHD2, Polyclonal Antibody (Cat# AAA28333)

Full Name

[KO Validated] CHCHD2 Rabbit pAb

Gene Names

CHCHD2; C7orf17

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SH-SY5Y cells using anti-STRIP1 antibody (AAA19927).Overlay histogram showing SH-SY5Y cells stained with AAA19927 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STRIP1 Antibody (AAA19927, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of STRIP1 using anti-STRIP1 antibody (AAA19927).STRIP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STRIP1 Antibody (AAA19927) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat C6 whole cell lysates,Lane 7: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRIP1 antigen affinity purified polyclonal antibody (#AAA19927) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STRIP1 at approximately 100 kDa. The expected band size for STRIP1 is at 96 kDa.)

STRIP1, Polyclonal Antibody (Cat# AAA19927)

Full Name

Anti-STRIP1 Antibody Picoband

Gene Names

STRIP1; FAM40A; FAR11A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-PKP2 antibody (AAA19770).Overlay histogram showing U87 cells stained with AAA19770 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKP2 Antibody (AAA19770, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PKP2 using anti-PKP2 antibody (AAA19770) and anti-Beta Tubulin antibody (M01857-3).PKP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PKP2 Antibody (AAA19770) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat heart tissue lysates,Lane 3: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKP2 antigen affinity purified polyclonal antibody (#AAA19770) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKP2 at approximately 92 kDa. The expected band size for PKP2 is at 97 kDa.)

PKP2, Polyclonal Antibody (Cat# AAA19770)

Full Name

Anti-PKP2 Antibody Picoband

Gene Names

PKP2; ARVD9

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-NANP antibody (AAA19931).Overlay histogram showing HepG2 cells stained with AAA19931 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NANP Antibody (AAA19931, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of NANP using anti-NANP antibody (AAA19931).NANP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NANP Antibody (AAA19931) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human A431 whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANP antigen affinity purified polyclonal antibody (#AAA19931) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (30 kDa.)

NANP, Polyclonal Antibody (Cat# AAA19931)

Full Name

Anti-NANP Antibody Picoband

Gene Names

NANP; HDHD4; C20orf147; dJ694B14.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U87 cells using anti-SUMF2 antibody (AAA19913).Overlay histogram showing U87 cells stained with AAA19913 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUMF2 Antibody (AAA19913, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of SUMF2 using anti-SUMF2 antibody (AAA19913).SUMF2 was detected in a paraffin-embedded section of human squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUMF2 Antibody (AAA19913) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUMF2 Antibody (AAA19913) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUMF2 Antibody (AAA19913) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUMF2 Antibody (AAA19913) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUMF2 Antibody (AAA19913) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUMF2 antigen affinity purified polyclonal antibody (#AAA19913) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUMF2 at approximately 36 kDa. The expected band size for SUMF2 is at 34 kDa.)

SUMF2, Polyclonal Antibody (Cat# AAA19913)

Full Name

Anti-SUMF2 Antibody Picoband

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

Cdk2, Monoclonal Antibody (Cat# AAA19658)

Full Name

Anti-Cdk2 Antibody Picoband (monoclonal, 6D5B5)

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 8. IF analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

PLD3, Polyclonal Antibody (Cat# AAA19440)

Full Name

Anti-PLD3 Antibody Picoband

Gene Names

PLD3; AD19; HUK4; HU-K4; SCA46

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ECSIT antibody (AAA19593).Overlay histogram showing HepG2 cells stained with AAA19593 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECSIT Antibody (AAA19593, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ECSIT, Polyclonal Antibody (Cat# AAA19593)

Full Name

Anti-ECSIT Antibody Picoband

Gene Names

ECSIT; SITPEC

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

CCL5, ELISA Kit (Cat# AAA17858)

Full Name

Rat CCL5 ELISA Kit

Gene Names

Ccl5; Scya5; Rantes

Reactivity

Rat

Applications

Sandwich ELISA

Pricing

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WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

EHD3, Monoclonal Antibody (Cat# AAA24062)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])

ROD1, Monoclonal Antibody (Cat# AAA25522)

Full Name

ROD1 (PTBP3, Polypyrimidine Tract-binding Protein 3, Regulator of Differentiation 1) (HRP)

Gene Names

PTBP3; ROD1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)

DGCR8, Polyclonal Antibody (Cat# AAA28373)

Full Name

DGCR8 Rabbit pAb

Gene Names

DGCR8; Gy1; pasha; DGCRK6; C22orf12

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

ARID3A, Monoclonal Antibody (Cat# AAA24729)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (Biotin)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat stomach using Slc39a11 Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

Slc39a11, Polyclonal Antibody (Cat# AAA28387)

Full Name

Slc39a11 Rabbit pAb

Gene Names

Slc39a11; 1810074D23Rik

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CDK6, Polyclonal Antibody (Cat# AAA28330)

Full Name

CDK6 Rabbit pAb

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

HCLS1, Monoclonal Antibody (Cat# AAA25122)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (FITC)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (Figure 7. IHC analysis of ERAB using anti-ERAB antibody (AAA11668).ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERAB Antibody (AAA11668) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ERAB, Polyclonal Antibody (Cat# AAA11668)

Full Name

Anti-ERAB Antibody

Gene Names

HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

AMACR/p504S, Monoclonal Antibody (Cat# AAA23906)

Full Name

AMACR/p504S (Prostate Cancer Marker)

Gene Names

AMACR; RM; RACE; CBAS4; P504S; AMACRD

Reactivity

Human. Others not known.

Applications

Western Blot, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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DDX1, Monoclonal Antibody (Cat# AAA19378)

Full Name

Anti-DDX1 Antibody (monoclonal, 3I10)

Gene Names

DDX1; DBP-RB; UKVH5d

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-IFT74 antibody (AAA19914).Overlay histogram showing JK cells stained with AAA19914 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IFT74 Antibody (AAA19914, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of IFT74 using anti-IFT74 antibody (AAA19914).IFT74 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT74 Antibody (AAA19914) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT74 Antibody (AAA19914) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT74 Antibody (AAA19914) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT74 Antibody (AAA19914) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IFT74 Antibody (AAA19914) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT74 antigen affinity purified polyclonal antibody (#AAA19914) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFT74 at approximately 69 kDa. The expected band size for IFT74 is at 69 kDa.)

IFT74, Polyclonal Antibody (Cat# AAA19914)

Full Name

Anti-IFT74 Antibody Picoband

Gene Names

IFT74; CMG1; CCDC2; CMG-1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HL-60 cells using anti-SSBP1 antibody (AAA19836).Overlay histogram showing HL-60 cells stained with AAA19836 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSBP1 Antibody (AAA19836, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SSBP1 using anti-SSBP1 antibody (AAA19836).SSBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human A549 whole cell lysates,Lane 6: human A375 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (#AAA19836) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.)

SSBP1, Polyclonal Antibody (Cat# AAA19836)

Full Name

Anti-SSBP1 Antibody Picoband

Gene Names

SSBP1; SSBP; mtSSB; Mt-SSB; SOSS-B1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HeLa cells using anti-SLU7 antibody (AAA19583).Overlay histogram showing HeLa cells stained with AAA19583 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLU7 Antibody (AAA19583, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SLU7, Polyclonal Antibody (Cat# AAA19583)

Full Name

Anti-SLU7 Antibody Picoband

Gene Names

SLU7; 9G8; hSlu7

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 8. IF analysis of CLASP1 using anti-CLASP1 antibody (AAA19528).CLASP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-CLASP1 Antibody (AAA19528) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

CLASP1, Polyclonal Antibody (Cat# AAA19528)

Full Name

Anti-CLASP1 Antibody Picoband

Gene Names

CLASP1; MAST1

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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MDH2, Polyclonal Antibody (Cat# AAA19527)

Full Name

Anti-MDH2 Antibody Picoband

Gene Names

MDH2; MDH; MOR1; M-MDH; MGC:3559

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using RPS7 antibody at dilution of 1:100 (40x lens).)

RPS7, Polyclonal Antibody (Cat# AAA28112)

Full Name

RPS7 Polyclonal Antibody

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western Blot analysis of CTNS expression in PC-12)

Cystinosin, Monoclonal Antibody (Cat# AAA24774)

Full Name

Cystinosin (CTNS) (Biotin)

Gene Names

CTNS; PQLC4; SLC66A4; CTNS-LSB

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA25821)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (PE)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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PRPF3, Polyclonal Antibody (Cat# AAA28150)

Full Name

PRPF3 Polyclonal Antibody

Gene Names

PRPF3; PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody at dilution of 1:100 (40x lens).)

HSPH1, Polyclonal Antibody (Cat# AAA28100)

Full Name

HSPH1 Polyclonal Antibody

Gene Names

HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)

DDX56, Monoclonal Antibody (Cat# AAA25666)

Full Name

DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (PE)

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IP (Immunoprecipitation) (LAMP1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-LAMP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LAMP1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 113 kDa)

LAMP1, Monoclonal Antibody (Cat# AAA27745)

Full Name

Recombinant Anti-LAMP1 Antibody, Rabbit Monoclonal

Gene Names

LAMP1; LAMPA; CD107a; LGP120

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein A

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of NSF using anti-NSF antibody (AAA19136).NSF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSF Antibody (AAA19136) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

NSF, Polyclonal Antibody (Cat# AAA19136)

Full Name

Anti-NSF Picoband antibody

Gene Names

NSF; SKD2; SEC18

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-RPS25 antibody (AAA19920).Overlay histogram showing HL-60 cells stained with AAA19920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS25 Antibody (AAA19920, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RPS25 using anti-RPS25 antibody (AAA19920).RPS25 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS25 Antibody (AAA19920) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS25 antigen affinity purified polyclonal antibody (#AAA19920) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS25 at approximately 17 kDa. The expected band size for RPS25 is at 14 kDa.)

RPS25, Polyclonal Antibody (Cat# AAA19920)

Full Name

Anti-RPS25 Antibody Picoband

Gene Names

RPS25; S25

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

CD115, ELISA Kit (Cat# AAA17901)

Full Name

Human CD115 ELISA Kit

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human

Applications

Sandwich ELISA

Pricing

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WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in PC-12.)

LRRN1, Monoclonal Antibody (Cat# AAA26284)

Full Name

LRRN1 (Leucine Rich Repeat Neuronal 1, FIGLER3, KIAA1497, NLRR-1, LRCH4) (Biotin)

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

RPS23, Monoclonal Antibody (Cat# AAA24639)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) APC

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)

RAB7B, Monoclonal Antibody (Cat# AAA25811)

Full Name

RAB7B (Ras-related Protein Rab-7b, RAB7) (PE)

Gene Names

RAB7B; RAB7

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

SAV1, Monoclonal Antibody (Cat# AAA25535)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (HRP)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody at dilution of 1:100 (40x lens).)

HADH, Polyclonal Antibody (Cat# AAA10684)

Full Name

HADH Polyclonal Antibody

Gene Names

HADH; HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

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WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

EHD3, Monoclonal Antibody (Cat# AAA24493)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) APC

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of OPA1 expression in transfected 293T cell line by OPA1 monoclonal antibody (M06), clone 8A32.Lane 1: OPA1 transfected lysate (Predicted MW: 111.6 KDa).Lane 2: Non-transfected lysate.)

OPA1, Monoclonal Antibody (Cat# AAA26020)

Full Name

OPA1 (Pptic Atrophy 1 (Autosomal Dominant), FLJ12460, KIAA0567, MGM1, NPG, NTG, LargeG) (AP)

Gene Names

OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14

Applications

Western Blot

Purity

Purified

Pricing

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Application Data (Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)

DNAJB6, Monoclonal Antibody (Cat# AAA24488)

Full Name

DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) APC

Gene Names

DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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SDS-PAGE (SDS-PAGE Analysis Purified ARF1 Mouse Monoclonal Antibody (ARF1/2117). Confirmation of Integrity and Purity of the Antibody.)

ARF1, Monoclonal Antibody (Cat# AAA23921)

Full Name

ARF1 (Golgi Apparatus Marker)

Gene Names

ARF1; PVNH8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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10000 results for "Recombinant Proteins" - showing 1400-1450

Cystinosin, Monoclonal Antibody (Cat# AAA25365)

SMAD2, Monoclonal Antibody (Cat# AAA26029)

GYG1, Monoclonal Antibody (Cat# AAA24085)

BAF60a, Polyclonal Antibody (Cat# AAA28224)

STIP1, Monoclonal Antibody (Cat# AAA26414)

ACBD3, Monoclonal Antibody (Cat# AAA25593)

LDB3, Monoclonal Antibody (Cat# AAA25143)

CHCHD2, Polyclonal Antibody (Cat# AAA28333)

STRIP1, Polyclonal Antibody (Cat# AAA19927)

PKP2, Polyclonal Antibody (Cat# AAA19770)

NANP, Polyclonal Antibody (Cat# AAA19931)

SUMF2, Polyclonal Antibody (Cat# AAA19913)

Cdk2, Monoclonal Antibody (Cat# AAA19658)

PLD3, Polyclonal Antibody (Cat# AAA19440)

ECSIT, Polyclonal Antibody (Cat# AAA19593)

CCL5, ELISA Kit (Cat# AAA17858)

EHD3, Monoclonal Antibody (Cat# AAA24062)

ROD1, Monoclonal Antibody (Cat# AAA25522)

DGCR8, Polyclonal Antibody (Cat# AAA28373)

ARID3A, Monoclonal Antibody (Cat# AAA24729)

Slc39a11, Polyclonal Antibody (Cat# AAA28387)

CDK6, Polyclonal Antibody (Cat# AAA28330)

HCLS1, Monoclonal Antibody (Cat# AAA25122)

ERAB, Polyclonal Antibody (Cat# AAA11668)

AMACR/p504S, Monoclonal Antibody (Cat# AAA23906)

DDX1, Monoclonal Antibody (Cat# AAA19378)

IFT74, Polyclonal Antibody (Cat# AAA19914)

SSBP1, Polyclonal Antibody (Cat# AAA19836)

SLU7, Polyclonal Antibody (Cat# AAA19583)

CLASP1, Polyclonal Antibody (Cat# AAA19528)

MDH2, Polyclonal Antibody (Cat# AAA19527)

RPS7, Polyclonal Antibody (Cat# AAA28112)

Cystinosin, Monoclonal Antibody (Cat# AAA24774)

RPS23, Monoclonal Antibody (Cat# AAA25821)

PRPF3, Polyclonal Antibody (Cat# AAA28150)

HSPH1, Polyclonal Antibody (Cat# AAA28100)

DDX56, Monoclonal Antibody (Cat# AAA25666)

LAMP1, Monoclonal Antibody (Cat# AAA27745)

NSF, Polyclonal Antibody (Cat# AAA19136)

RPS25, Polyclonal Antibody (Cat# AAA19920)

CD115, ELISA Kit (Cat# AAA17901)

LRRN1, Monoclonal Antibody (Cat# AAA26284)

RPS23, Monoclonal Antibody (Cat# AAA24639)

RAB7B, Monoclonal Antibody (Cat# AAA25811)

SAV1, Monoclonal Antibody (Cat# AAA25535)

HADH, Polyclonal Antibody (Cat# AAA10684)

EHD3, Monoclonal Antibody (Cat# AAA24493)

OPA1, Monoclonal Antibody (Cat# AAA26020)

DNAJB6, Monoclonal Antibody (Cat# AAA24488)

ARF1, Monoclonal Antibody (Cat# AAA23921)

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