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10000 results for "Recombinant Proteins" - showing 3100-3150
ELISA
(Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).)
HIV-1 p24, Monoclonal Antibody (Cat# AAA40477)
Full Name
HIV-1 p24 Antibody [7F4]
Reactivity
Virus
Applications
Western Blot
Purity
HIV-1 p24 antibody is supplied as protein A purified IgG1.
Pricing
ELISA
(ELISA: MERS Spike S2 Antibody (1ug/ml) specifically recognizes MERS Spike and Spike S2 recombinant protein, but not interacted MERS Spike S1 and RBD recombinant protein in ELISA.)
MERS-CoV Spike S2 Subunit, Monoclonal Antibody (Cat# AAA48360)
Full Name
MERS-CoV Spike S2 Subunit antibody
Reactivity
MERS-CoV
Applications
Western Blot
Purity
By protein-A affinity chromatography
Pricing
Application Data
(The OmniKine Human CXCL-16 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CXCL-16 proteins within the range of 12-3000 pg/ml.)
CXCL-16, ELISA Kit (Cat# AAA315603)
Full Name
CXCL-16 (Human) OmniKine ELISA Kit
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human
Pricing
SDS-PAGE
(SDS-PAGE)
Vascular Endothelial Growth Factor C (VEGFC), Recombinant Protein (Cat# AAA152049)
Full Name
Eukaryotic Vascular Endothelial Growth Factor C (VEGFC)
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
(Anti-HIF1 beta Picoband antibody, AAA46024-2.jpgAll lanes: Anti HIF1 beta (AAA46024) at 0.5ug/mlLane 1: Hela Whole Cell Lysate at 40ugLane 2: 293T Whole Cell Lysate at 40ugLane 3: Jurkat Whole Cell Lysate at 40ugLane 4: U87 Whole Cell Lysate at 40ugLane5: Colo320 Whole Cell Lysate at 40ugPredicted bind size: 87KDObserved bind size: 87KD)
Aryl hydrocarbon receptor nuclear translocator, Polyclonal Antibody (Cat# AAA46024)
Full Name
Anti-HIF1 beta Antibody
Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
SDS-PAGE
(SDS-PAGE)
Phospholipase A2 Group VII (LpPLA2), Recombinant Protein (Cat# AAA152257)
Full Name
Recombinant Phospholipase A2 Group VII (LpPLA2)
Gene Names
Pla2g7; PAF-AH; R75400
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded NCI-H226 (left) and HEK 293 transfected with PD-L1(right). Cell was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody for 15 min at room temperature. Secondary: Goat Anti-Rabbit IgG (H&L))
PD-L1, Monoclonal Antibody (Cat# AAA291002)
Full Name
Recombinant PD-L1 antibody [AC37]
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Pricing
SDS-PAGE
(SDS-PAGE)
Caspase 2 (CASP2), Recombinant Protein (Cat# AAA152166)
Full Name
Recombinant Caspase 2 (CASP2)
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant MEF2C protein using MEF2C antibody)
MEF2C, Monoclonal Antibody (Cat# AAA107191)
Full Name
MEF2C antibody
Gene Names
MEF2C; DEL5q14.3; C5DELq14.3
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
MEF2C antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with ATGA0480 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
AKT1/3, Monoclonal Antibody (Cat# AAA48945)
Full Name
AKT1/3 antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
ALDH2, Monoclonal Antibody (Cat# AAA48263)
Full Name
ALDH2 antibody
Gene Names
ALDH2; ALDM; ALDHI; ALDH-E2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
Amino Acid Info
Chemokine C-X3-C-Motif Receptor 1 (CX3CR1), Recombinant Protein (Cat# AAA153453)
Full Name
Recombinant Chemokine C-X3-C-Motif Receptor 1 (CX3CR1)
Gene Names
CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
Amino Acid Info
Eukaryotic Parathyroid Hormone (PTH), Protein (Cat# AAA153186)
Full Name
Eukaryotic Parathyroid Hormone (PTH)
Gene Names
PTH; PTH1
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohistochemistry)
(TCP1 beta was detected in paraffin-embedded sections of human testis tissues using rabbit anti- TCP1 beta Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
TCP1 beta, Polyclonal Antibody (Cat# AAA46640)
Full Name
Anti-TCP1 beta Antibody
Gene Names
CCT2; CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
ELISA
(Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein ELISA DataImmobilized Anti-CD28 Antibody, hFc Tag at 2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA.)
CD28, Recombinant Protein (Cat# AAA76181)
Full Name
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein Recombinant
Gene Names
CD28; Tp44
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(N-Acetyltransferase 2 (NAT2)) is an enzyme that plays an important role in metabolism and detoxification of many compounds including drugs and environmental carcinogens through chemical modification of the amine group with an acetyl group. Cytochrome P4502E1 (CYP2E1) and NAT2 are related to chronic obstructive pulmonary disease and CYP2E1 can bind to NAT2. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat NAT2 and recombinant mouse CYP2E1. Briefly, NAT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CYP2E1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NAT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat NAT2 and recombinant mouse CYP2E1 was shown in Figure 1, the EC50 for this effect is 0.096 ug/mL.)
N-Acetyltransferase 2 (NAT2), Active Protein (Cat# AAA161924)
Full Name
Active N-Acetyltransferase 2 (NAT2)
Gene Names
Nat2; Nat2a
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of CD5 using anti-CD5 antibody (AAA125903).CD5 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD5 Antibody (AAA125903) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD5, Monoclonal Antibody (Cat# AAA125903)
Full Name
Anti-CD5 Antibody (monoclonal, 11B4)
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein SPR DataBiotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).)
TRAIL R1/DR4/TNFRSF10A, Recombinant Protein (Cat# AAA76276)
Full Name
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein Recombinant
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(Cluster Of Differentiation 200 (CD200), also known as OX-2 or MOX1/MOX2, is a membrane glycoprotein belonging to the immunoglobulin superfamily. This protein is widely expressed in various cell types, including neurons, endothelial cells, certain immune cells (such as B cells, dendritic cells, T cell subsets), and various tumor cells. CD200 is a multifaceted protein with important roles in immune regulation, neuronal function, and disease pathogenesis. Besides, Cluster Of Differentiation 8a (CD8a) has been identified as an interactor of CD200, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CD200 and recombinant human CD8a. Briefly, CD200 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CD8a-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD200 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat CD200 and recombinant human CD8a was shown in Figure 1, the EC50 for this effect is 0.09 ug/mL.)
Cluster Of Differentiation 200 (CD200), Active Protein (Cat# AAA161800)
Full Name
Active Cluster Of Differentiation 200 (CD200)
Gene Names
Cd200; Mox2; Cspmo2; MRCOX2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal (HLA-DQA1/2866R) followed by goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
HLA-DQ, Monoclonal Antibody (Cat# AAA214758)
Full Name
HLA-DQ (MHC II)
Gene Names
HLA-DQA1; DQA1; DQ-A1; CELIAC1; HLA-DQA
Reactivity
Human, Pig. Others not known.
Applications
Immunohistochemistry, Flow Cytometry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using C1QA Mouse Monoclonal Antibody (C1QA/2954). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
C1QA/Complement C1q A-Chain, Monoclonal Antibody (Cat# AAA215222)
Full Name
C1QA/Complement C1q A-Chain
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman ALT AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Alanine Aminotransferase (ALT), Monoclonal Antibody (Cat# AAA151506)
Full Name
Monoclonal Antibody to Alanine Aminotransferase (ALT)
Gene Names
GPT; AAT1; ALT1; GPT1
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Cytokeratin 18 using anti-Cytokeratin 18 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human A549 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hours at RT. The membrane was incubated with mouse anti-Cytokeratin 18 antigen affinity purified monoclonal antibody at 0.5 μg/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cytokeratin 18 at approximately 48KD. The expected band size for Cytokeratin 18 is at 48KD.)
Cytokeratin 18, Monoclonal Antibody (Cat# AAA125501)
Full Name
Anti-Cytokeratin 18 Antibody (Monoclonal, 7I6)
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot Analysis of K562 and PC3 cell lysate using SERBP1 Mouse Monoclonal Antibody (SERBP1/3509).)
SERBP1, Monoclonal Antibody (Cat# AAA215398)
Full Name
SERBP1/PAI-RBP1
Gene Names
SERBP1; CGI-55; CHD3IP; HABP4L; PAIRBP1; PAI-RBP1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using PMEL/gp100 Mouse Monoclonal Antibody (PMEL/2037).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
gp100/Melanosome/PMEL17/SILV, Monoclonal Antibody (Cat# AAA214682)
Full Name
gp100/Melanosome/PMEL17/SILV (Melanoma Marker)
Gene Names
PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Confocal Immunofluorescence of MCF-7 Cells EpCAM Mouse Monoclonal Antibody (EGP40/1798) labeled with CF488 (Green); Reddot is used to label the nuclei.)
Ep-CAM/CD326, Monoclonal Antibody (Cat# AAA214435)
Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-STIL/SIL antibody (AAA19302).Overlay histogram showing SiHa cells stained with AAA19302 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STIL/SIL Antibody (AAA19302, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STIL/SIL, Polyclonal Antibody (Cat# AAA19302)
Full Name
Anti-STIL/SIL Antibody
Gene Names
STIL; SIL; MCPH7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
Bioactivity
(Caspase-2 (CASP2) is a 3032 kDa member of the peptidase C14A/IL1 beta-converting family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Based on the length of its prodomain, caspase-2 has been considered to be an initiator caspase. Human procaspase-2 is a 4851 kDa, 452 amino acid (aa) protein. It is known to exist as a disulfide-linked homodimer via covalent linkage at Cys436. But this dimeric state may not be sufficient for (auto) activation. Actual activation may occur following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome. The activity assay of recombinant human CASP2 was measured by its ability to cleave the fluorogenic peptide substrate Ac-VDVAD-AFC. The reaction was performed in 25 mM HEPES, 0.1% (w/v) CHAPS, 10 mM dithiothreitol (DTT), pH 7.5 (Assay Buffer). The CASP2 was diluted to 3 ug/ml by assay buffer and incubated at room temperature for 15min. The reaction was initiated by adding 50 ul 3 ug/ml CASP2 to 50 ul of 200 uM substrate and then read at excitation and emission wavelengths of 400 nm and 505 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human CASP2 is 2100 pmol/min/ug.)
Caspase 2 (CASP2), Active Protein (Cat# AAA161718)
Full Name
Active Caspase 2 (CASP2)
Gene Names
CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme H's functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type. Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1-18), a propeptide (residues 19-20) and a mature chain (residues 21-246). The purified recombinant human Granzyme H consists of residues 19 to 246 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 uL of various concentrations of GZMH (diluted by Assay Buffer) to 50 uL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMH, replaced with 50 uL assay buffer. Incubated at 25 degree C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme H is >300 pmol/min/ug.)
Granzyme H (GZMH), Active Protein (Cat# AAA161935)
Full Name
Active Granzyme H (GZMH)
Gene Names
GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(The cytochrome P450 enzyme CYP2E1 catalyzes the oxidative metabolism of many solvents and other small organic molecules. CYP2E1 is expressed in adult and fetal human liver in addition to extrahepatic tissues such as lung and placenta. Treatment of primary cultures of human hepatocytes with ethanol induces CYP2E1 protein, and this is consistent with the finding that hepatic CYP2E1 protein and mRNA levels are increased in individuals with alcoholism. Although only a few drugs (e.g., acetaminophen have been identified as substrates for CYP2E1, many low molecular weight procarcinogens are activated by this cytochrome P450 (P450). Chlorzoxazone 6-hydroxylation, N-nitrosodimethylamine N-demethylation and p-nitrophenol hydroxylation can be used to measure the catalytic activity of CYP2E1. Thus, the recombinant mouse CYP2E1 activity was measured by its ability to hydroxylate p-nitrophenol to p-nitrocatechol. The reaction was performed in 50 mM potassium phosphate, pH 7.4 (Assay Buffer), initiated by addition 20 uL of 500 ug/ml CYP2E1 to 10 uL of 5 mM substrate p-nitrophenol and 30 ul of 26 mM NADPH in a total volume of 500 ul. Incubated at 37 degree C for 30min, then read at a wavelength of 535 nm after acidification of the reaction mixture with trichloroacetic acid followed by neutralization using 2 M NaOH.)
Cytochrome P450 2E1 (CYP2E1), Active Protein (Cat# AAA161819)
Full Name
Active Cytochrome P450 2E1 (CYP2E1)
Gene Names
Cyp2e1; Cyp2e
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-CD39/Entpd1 antibody (AAA127167).Overlay histogram showing ANA-1 cells stained with AAA127167 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD39/Entpd1 Antibody (AAA127167, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
CD39/Entpd1, Polyclonal Antibody (Cat# AAA127167)
Full Name
Anti-CD39/Entpd1 Antibody Picoband
Gene Names
Entpd1; Cd39; AA408691; NTPDase-1; 2610206B08Rik
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CXCR2 antibody (AAA126029).Overlay histogram showing THP-1 cells stained with AAA126029 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR2 Antibody (AAA126029, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CXCR2, Polyclonal Antibody (Cat# AAA126029)
Full Name
Anti-CXCR2 Antibody Picoband
Gene Names
CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of ITGAV using anti-ITGAV antibody (AAA126896).ITGAV was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-ITGAV Antibody (AAA126896) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
Integrin alpha V/ITGAV, Monoclonal Antibody (Cat# AAA126896)
Full Name
Anti-Integrin alpha V/ITGAV Antibody Picoband (monoclonal, 8B10H2)
Gene Names
ITGAV; CD51; MSK8; VNRA; VTNR
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of Lamin A+C/LMNA using anti-Lamin A+C/LMNA antibody (AAA126868).Lamin A+C/LMNA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Lamin A+C/LMNA Antibody (AAA126868) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
Lamin A+C/LMNA, Monoclonal Antibody (Cat# AAA126868)
Full Name
Anti-Lamin A+C/LMNA Antibody Picoband (monoclonal, 5F3C12)
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Standard Curve (Sample)
EGF, CLIA Kit (Cat# AAA318566)
Full Name
EGF (Murine) LumiAb ELISA Kit
Gene Names
Egf; AI790464
Reactivity
Mouse
Pricing
Standard Curve (Sample)
beta-NGF, ELISA Kit (Cat# AAA79353)
Full Name
Murine beta-NGF Pre-Coated ELISA Kit
Gene Names
Ngf; Ngfb
Reactivity
Murine
Pricing
Amino Acid Info
Histone H4 (H4), Recombinant Protein (Cat# AAA153328)
Full Name
Recombinant Histone H4 (H4)
Gene Names
Hist1h4m; Gm11275
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
C ReProtein (CRP), Recombinant Protein (Cat# AAA150676)
Full Name
Recombinant C Reactive Protein (CRP)
Reactivity
Equus caballus; Equine (Horse)
Applications
Western Blot
Purity
> 90%
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Insulin Like Growth Factor Binding Protein 7 (IGFBP7), Recombinant Protein (Cat# AAA150691)
Full Name
Recombinant Insulin Like Growth Factor Binding Protein 7 (IGFBP7)
Gene Names
IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
Applications
Western Blot
Purity
>80%
Pricing
Rotavirus VP4, Recombinant Protein (Cat# AAA24011)
Full Name
Rotavirus VP4 protein (His tag)
Purity
>90% pure by SDS-PAGE
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using ANGPTL3 Antibody at dilution of 1:100 (40x lens).)
ANGPTL3, Polyclonal Antibody (Cat# AAA37249)
Full Name
ANGPTL3 Polyclonal Antibody
Gene Names
ANGPTL3; ANL3; ANG-5; FHBL2; ANGPT5
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
Application Data
(The OmniKine Human M-CSF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human M-CSF proteins within the range of 16-1000 pg/ml.)
M-CSF, ELISA Kit (Cat# AAA315739)
Full Name
M-CSF (Human) OmniKine ELISA Kit
Gene Names
CSF1; MCSF; CSF-1
Reactivity
Human
Pricing
Amino Acid Info
Interleukin 23 Receptor (IL23R), Recombinant Protein (Cat# AAA153618)
Full Name
Recombinant Interleukin 23 Receptor (IL23R)
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Colony Stimulating Factor 3, Granulocyte (GCSF), Recombinant Protein (Cat# AAA150651)
Full Name
Recombinant Colony Stimulating Factor 3, Granulocyte (GCSF)
Applications
Western Blot
Purity
>95%
Pricing
Application Data
(The OmniKine Human PDGF-AB ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PDGF-AB proteins within the range of 16-1000 pg/ml.)
PDGF-AB, ELISA Kit (Cat# AAA315824)
Full Name
PDGF-AB (Human) OmniKine ELISA Kit
Gene Names
PDGFA; PDGF1; PDGF-A
Reactivity
Human
Pricing
Application Data
(The OmniKine Human ST2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human ST2 proteins within the range of 32-2000 pg/ml.)
ST2, ELISA Kit (Cat# AAA315830)
Full Name
ST2 (Human) OmniKine ELISA Kit
Gene Names
IL1RL1; T1; ST2; DER4; ST2L; ST2V; FIT-1; IL33R
Reactivity
Human
Pricing
Amino Acid Info
Tissue Plasminogen Activator (tPA), Recombinant Protein (Cat# AAA153352)
Full Name
Recombinant Tissue Plasminogen Activator (tPA)
Gene Names
PLAT; TPA; T-PA
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PRKAR1B protein (Right) or empty vector (Left) detected with PRKAR1B antibody)
PRKAR1B, Monoclonal Antibody (Cat# AAA107361)
Full Name
PRKAR1B antibody
Gene Names
PRKAR1B; PRKAR1
Applications
Western Blot, Immunofluorescence
Purity
PRKAR1B antibody was purified by affinity chromatography.
Pricing
Application Data
(Western blot detection of CDC2/CDK1 in MCF7&Hela cell lysates using CDC2/CDK1 antibody (1:1000 diluted). Predicted band size: 34KDa Observed band size: 34KDa.)
CDC2/CDK1, Monoclonal Antibody (Cat# AAA111301)
Full Name
CDC2/CDK1 Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
Affinity purified
Pricing
Bioactivity
(HTRF assay for AMPK Complex (A1+B1+G1) activity 1 uM STK S1 substrate was incubated with different concentrations of AMPK Complex (A1+B1+G1) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 50 uM AMP and 100 uM ATP for 1 hr. The 10 ul detection reagents containing anti-STK antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
AMPK Complex (A1+B1+G1), Active Protein (Cat# AAA60345)
Full Name
Recombinant AMPK Complex (A1+B1+G1) protein
Purity
>=90%
Pricing