Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SIRT1 recombinant protein
Recombinant SIRT1 (193-741) protein
Purity
Recombinant SIRT1 (193-741) protein that includes amino acids 193-741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa.The purity ofThe protein is > 80% b
Synonyms
SIRT1; N/A; Recombinant SIRT1 (193-741) protein; Sirtuin 1; SIRT-1; SIR2-Like Protein; SIR2L1; SIR2alpha; SIRT1 recombinant protein
Host
E Coli
Purity/Purification
Recombinant SIRT1 (193-741) protein that includes amino acids 193-741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa.The purity ofThe protein is > 80% b
Form/Format
Recombinant SIRT1 (193-741) protein is supplied in 25mM Tris pH8.0, 150mM NaCl, 10% glycerol, and 0.5mM TCEP.
Protein Species
Human
Tag
GST-Tag
Notes
Recombinant SIRT1 protein is useful for histone deacetylase assays and the study of DNA Repair.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SDS-PAGE
(Recombinant SIRT1 (193-741)12.5% SDS-PAGE with Coomassie Blue staining.MW: 101.5 kDaPurity: >80%)
SDS-PAGE
(Recombinant SIRT1 (193-741)12.5% SDS-PAGE with Coomassie Blue staining.MW: 101.5 kDaPurity: >80%)
Related Product Information for SIRT1 recombinant protein
Short Description: Recombinant SIRT1 (193-741) protein includes amino acids 193-741 of human SIRT1 protein (accession number NM_012238.4); it was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa. The purity of the protein is > 60% by SDS-PAGE. It is useful for histone deacetylase assays and the study of DNA Repair.
Background: SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD+-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
Background: SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD+-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
Product Categories/Family for SIRT1 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 101.5kDa.
Similar Products
Product Notes
The SIRT1 (Catalog #AAA59738) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SIRT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.