IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.)
Rabbit anti-Human CDH1/E Cadherin Polyclonal Antibody | anti-CDH1 antibody
CDH1/E Cadherin Rabbit anti-Human Polyclonal Antibody
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry)
(CDH1/E Cadherin Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(CDH1/E Cadherin Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(CDH1/E Cadherin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(CDH1/E Cadherin Antibody-Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (20ug))
WB (Western Blot)
(CDH1/E Cadherin Antibody-Immunoprecipitating CDH1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CDH1 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CDH1 Antibody (6ug) + HEK293 whole cell lysate (500ug) Lane 3: HEK293 whole cell lysate (20ug))
WB (Western Blot)
(CDH1/E Cadherin Antibody-Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa)
WB (Western Blot)
(CDH1/E Cadherin Antibody-Western Blot Positive WB detected in: 293 whole cell lysate All Lanes: CDH1 antibody at 2.9ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 98, 91 KDa Observed band size: 125 KDa)
ICC (Immunocytochemistry)
(CDH1/E Cadherin Antibody-Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
ICC (Immunocytochemistry)
(CDH1/E Cadherin Antibody-Immunofluorescence staining of HepG2 cells diluted at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NCBI and Uniprot Product Information
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Product Notes
The CDH1 cdh1 (Catalog #AAA21361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDH1/E Cadherin Rabbit anti-Human Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDH1/E Cadherin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the CDH1 cdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDH1/E Cadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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